Résumé
Objective To investigate the possible protective effect of adenoviral vector expressing interleukin-1β (IL-1β) small hairpin RNA (shRNA) on spinal cord injury (SCI) and its mechanism in rats.Methods Forty-eight adult male Sprague-Dawley rats were randomly assigned to 4 groups including the Sham, the Vehicle,the Ad-GFP and the Ad-shIL-1β groups.SCI was induced by epidural compression.Motor function of hind limbs was evaluated by Basso-Beattie-Bresnahan (BBB) score, the expressions of green fluorescence in injured spinal cord tissue were observed by fluorescence microscope.Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence were also performed.Results The expressions of green fluorescence in injured spinal cord tissue were observed in the Ad-GFP and Ad-shIL-1β groups one day after SCI.Significant functional improvement was observed in the Ad-shIL-1β group (8.17 ± 1.17, 10.17 ± 0.98 and 11.33 ± 0.82, respectively) compared to the Vehicle (4.00 ± 0.89, 5.67 ± 1.03 and 6.17 ± 1.17, respectively) and Ad-GFP (3.83 ± 0.98, 5.33 ± 1.21 and 5.67 ± 1.03, respectively) groups at 7, 14 and 21 days after SCI (P ＜ 0.05).Rats in the Ad-shIL-1β group had less neuronal loss 21 days after SCI.In addition, IL-1β downregulation significantly decreased IL-1β, tumor necrosis factor-or (TNF-α) and IL-6 levels (138.83 ± 7.96,143.38 ± 10.20 and 120.43 ± 9.79 in Ad-shIL-1β group;169.33 ± 11.45, 172.33 ± 8.26 and 163.00 ± 9.57 in Vehicle group;172.83 ± 10.85,167.48 ± 8.19 and 159.48 ± 10.98 in Ad-GFP group, respectively) one day after SCI (P ＜ 0.05).Conclusion This study demonstrated that the IL-1β downregulation may have potential therapeutic benefits for improving the outcomes after SCI.
Résumé
Objective To investigate health education on the quality of life and prevention of peritonitis in peritoneal dialysis patients. Methods Total of 218 cases of peritoneal dialysis patients were divided into two groups accorded to duration, observation group (117 cases) and control group (101 cases), the observation group plus health education treat-ment measures above contral group, used the SF-36 scale evaluation of the quality of life before and after intervention was observed peritonitis and other complications, analyzed peritonitis incentives. Results The SF-36 scale body pain, physical function, physical health, mental health, general health, energy, emotional function, social function scores of the upward trend in quality of life after intervention in observation group were significantly improved (P <0.05),that in the control group were not significant improvement (P>0.05), the observation group intervention for 6 months and 12 months each dimension scores were significantly higher(P<0.05). Within 12 months of follow-up peritonitis rates in two group were 6.84% and 18.81% , the overall incidence of peritonitis observation group were significantly lower than the control group (P<0.05), the incidence increasesd with time. Observation group of patients with peritonitis main incen-tives were digestive diseases, unqualified aseptic tehnique poor personal hygiene, catheter exit infection, other parts of the proportion of infections were significantly lower than the control group (P <0.05). Observation group of other peri-toneal dialysis complication rate was significantly lower than the control group (P<0.05). Conclusion Health education effectively improve the knowledge of the peritoneal dialysis patient's quality of life and prevent complications and re-duce the incidence of peritonitis.