The study in immunology after skin transplantation / 中华烧伤杂志

Skin grafting has been one of the most important approaches for covering burn wounds, however long-term survival of allogeneic or xenogeneic skin graft is currently not successful. How to induce immune tolerance for life-time survival of allogeneic or xenogeneic skin graft is still remote objective to be solved. However, clinicians and scientists in China have worked very hard and made great contribution to this field during the past 50 years, no matter how difficult it is. They are the respected pioneers in the understanding of immunological change in "Chinese Method" skin grafting, its local immune tolerance, immunology of pre-treatment of skin graft, etc. Herein, the most outstanding and impressive progresses in immunological responses after skin grafting in the past 50 years in China have been reviewed and presented for memory, for future and for extending a salute.

Experimental study on the antibacterial effects of zirconium phosphate gauze loaded with silver on the common bacteria on rat burn wound / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the antibacterial effects of zirconium phosphate gauze loaded with silver on rat burn wounds seeded with commonly seen bacteria.</p><p><b>METHODS</b>Wistar rats were employed in the study and were scalded and infected. The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of zirconium phosphate loaded with silver were determined by double dilution in tubes. The effect on wound healing and the subeschar bacterial count of the rat burn wounds were observed after the wounds had been covered by gauze loaded with zirconium phosphate and silver, and also with the gauze which has been rinsed for 20 times.</p><p><b>RESULTS</b>The MIC of silver loaded zirconium phosphate on Staphylococcus aureus, Pseudomonas aeruginosa and E. coli were 8, 8 and 16 mg/L, respectively, while the MBC were 16, 8 and 32 mg/L, respectively. The subeschar bacterial count in the burn wounds with the gauze with silver loaded zirconium phosphate was ten times lower than that in those which were treated with gauze with SD-Ag and 100 times lower than that with ordinary gauze. But there was no difference in the bacterial count between the wounds which were treated with fresh gauze with silver loaded zirconium phosphate and that with the gauze which has been rinsed for 20 times (P > 0.05). Furthermore, wound healing seemed to be better with the gauze with silver loaded zirconium phosphate when compared with those by the other two kinds of gauze.</p><p><b>CONCLUSION</b>The silver loaded zirconium phosphate was found to be bacteriocidal against bacteria commonly seen in the burn wounds.</p>

Effect of ligustrazine on the changes of collagen and expression of transforming growth factor - ?1 in airway wall of asthmatic rats / 实用儿科临床杂志

Objective To observe the effect of ligustrazine on the changes of collagen, transforming growth factor- ?1(TGF- ?1) content in airway wall of asthmatic rats. Methods Forty SD rats were randomly divided mto normal group,model group, desametha-sone group, low - dose and high - dose of ligustrazine group, 8 rats in each group. The changes of collagen and TGF - ?1 content in the atrway wall were measured by the computerized image analysis system. Results The contents of collagen type Ⅲ and TGF -?1 in air-way wall in model group were significantly higher than controls group(P ail

An experimental study on the role of indirect antigen presentation pathway on the immunogenetic of epidermal cells / 中华烧伤杂志

<p><b>OBJECTIVE</b>To explore the role of indirect antigen presentation pathway on the immunogenecity of epidermal cells.</p><p><b>METHODS</b>Human epidermal cells (HEC), allogeneic human peripheral blood lymphocytes (PBL) and mononuclear cells (PBM, including monocytes) were isolated and cultured in vitro. HECs were transfected by human-originated CTLA4Ig-adenovirus vector. The CTLA4Ig expression was observed. Allogeneic PBLs or PBMs were added to the transfected and non-transfected HECs with simple cultured PBLs and PBMs as the control. The proliferation of PBL and PBM was determined by (3)H-TdR incooperation.</p><p><b>RESULTS</b>HECs could be successfully transfected by CTLA4Ig-adenovirus vector and expressed corresponding proteins. The non-transfected HECs could stimulate slight proliferation of allogeneic PBLs (P < 0.05) and stimulate remarkable proliferation of PBMs (including monocytes) (P < 0.05). The proliferation reaction of PBLs and PBMs decreased significantly (P < 0.05) after being stimulated by HEC which was modulated by CTLA4Ig genes.</p><p><b>CONCLUSION</b>Indirect antigen presentation pathway might play important roles in the HEC immunogenicity which could be evidently inhibited by CTLA4Ig.</p>