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1.
Zhongguo Zhong Yao Za Zhi ; (24): 2475-2479, 2015.
Article de Anglais | WPRIM | ID: wpr-284789

RÉSUMÉ

There are two types of Chinese medicine, i.e., "Shihu" and "Tiepi shihu", using the stems of species of Dendrobium (Orchidaceae) as original materials. In order to understand species of Dendrobium using in Chinese medicine, taxonomic investigation and revision were performed. Our results indicated that about sixty species of sect. Dendrobium, sect. Formosae, and Sect. Stachyobium, are used as raw materials of "Shihu". Seven species of Dendrobium moniliforme complex and four species in Dendrobium officinale complex were recognized.


Sujet(s)
Dendrobium , Classification , Médicaments issus de plantes chinoises , Classification
2.
Zhongguo Zhong Yao Za Zhi ; (24): 3663-3667, 2014.
Article de Chinois | WPRIM | ID: wpr-311012

RÉSUMÉ

Molecular identification of Chinese traditional medicine has come from laboratory research into application, but there are some misunderstandings and problems emerging after rapid development. In this paper, we discuss the usage principle, hot field and technology innovation in molecular identification of Chinese traditional medicine. And molecular identification of traditional Chinese medicine has scientific and objective basis, follows the certain systematic research background, and adopts practical principles to establish case by case multi-class identification system. In order to achieve rapid, on-site, high throughput, low cost of traditional Chinese medicine identification purpose, molecular identification technology is further developing for meet the actual needs and the laboratory results further transformation in the service of traditional Chinese medicine industry.


Sujet(s)
Chine , Médicaments issus de plantes chinoises , Chimie , Normes de référence , Médecine traditionnelle chinoise , Plantes médicinales , Chimie , Classification , Génétique , Contrôle de qualité
3.
Article de Chinois | WPRIM | ID: wpr-332714

RÉSUMÉ

This study was purpose to evaluate a new method and instrument for detecting platelet aggregation function, establish the reference intervals for PL-11 platelet analyzer, and evaluate its clinical application. The evaluation was based on the guidelines of Clinical and Laboratory Standards Institute (CLSI or NCCLS) and Clinical Laboratory Improvement Amendment 88. Intravenous blood samples anticoagulated with sodium citrate were detected by PL-11 platelet analyzer. The reference intervals were defined after statistic analysis. The clinical diagnostic significance of the PL-11 platelet analyzer was evaluated by testing the change rate of platelet maximum aggregation rate (MAR) of acute cerebral infarction (ACI) patients in the department of Neurology who took clopidogrel 7 d before and after. The result showed that all the parameters meet the standard of CLIA'88. The platelet MAR of 247 healthy volunteers which was induced by PLR-06, PLR-07, PLR-09 and PLR-10, was detected by the PL-11 platelet analyzer, respectively. The MAR is 58.8 ± 10.1 (%), 61.2 ± 11.8 (%), 51 ± 10.2 (%), 53.1 ± 9.2 (%), respectively. The MAR of ACI patients is significantly lower than that after taking clopidogrel. It is concluded that the PL-11 platelet analyzer is an ideal platelet function detector for early warning and diagnosis of thromboembolic disease, which is worthy to be extended and applied.


Sujet(s)
Adolescent , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Agrégation plaquettaire , Tests fonctionnels plaquettaires , Méthodes
4.
Chinese Journal of Virology ; (6): 126-131, 2013.
Article de Chinois | WPRIM | ID: wpr-339964

RÉSUMÉ

Gene therapy offers the promise of curing the HIV-infected patients. Specific, potent, and sustained short hairpin RNA (shRNA)-mediated gene silencing is crucial for the successful application of RNA interference technology to therapeutic interventions. To reduce the probability of viral escape mutants, in this study, we constructed lentiviral vector containing vpr and tat shRNA, respectively, furthermore the bispecific lentiviral vector harboring vpr and tat shRNA expression cassettes from U6 promotor and H1 promotor was cotransfected with recombinant plasmid expressing the vpr and tat gene. The result showed that the bispecific lentiviral vector plvx-vpr-tatshRNA could inhibit the vpr and tat effectively,with ratios of 89.20% and 62.00% respectively. When cotransfected with pNL4-3 in 293T cell, plvx-vpr-tatshRNA showed higher efficacy in down regulating the HIV NL4-3 packaging production than the plvx-vprshRNA or plvx-tatshRNA individually. MT4 cell clones transduced with recombinant lentiviral vectors were screened and challenged with HIV NL4-3. P24 ELISA test showed that MT4 transduced with the combinational lentiviral vector could inhibit virus replication efficiently.


Sujet(s)
Humains , Lignée cellulaire , Régulation négative , Thérapie génétique , Vecteurs génétiques , Génétique , Métabolisme , Infections à VIH , Thérapeutique , Virologie , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Génétique , Métabolisme , Lentivirus , Génétique , Métabolisme , Interférence par ARN , Petit ARN interférent , Génétique , Métabolisme , Utilisations thérapeutiques , Produits du gène tat du virus de l'immunodéficience humaine , Génétique , Métabolisme , Produits du gène vpr du virus de l'immunodéficience humaine , Génétique , Métabolisme
5.
Article de Chinois | WPRIM | ID: wpr-229853

RÉSUMÉ

<p><b>OBJECTIVE</b>To develop a new denaturing high performance liquid chromatograph (DHPLC)-based method to screen patients with EXT gene mutation and to study the gene mutation in three families with multiple exostoses.</p><p><b>METHODS</b>All the exons of EXT gene, including the intro-exon boundaries, were amplified by PCR. Linkage analysis and DHPLC screening were carried out to identify the mutations. DNA sequencing was used to confirm the mutations.</p><p><b>RESULTS</b>Two known splice site mutations, IVS2+1 G to A and IVS7+1 G to T, and two SNPs have been detected in EXT2 or EXT1 gene.</p><p><b>CONCLUSION</b>The transversions of IVS2+1 G to A and IVS7+1 G to T in EXT2 gene are suggested to be the disease-causing mutations and the DHPLC is a high throughout, sensitive, simple, quick, economical method to screen gene mutation in hereditary multiple exostosis.</p>


Sujet(s)
Adulte , Femelle , Humains , Mâle , Chromatographie en phase liquide à haute performance , Méthodes , Analyse de mutations d'ADN , Électrophorèse sur gel de polyacrylamide , Exons , Génétique , Maladie des exostoses multiples , Génétique , Mutation , N-acetylglucosaminyltransferase , Génétique
6.
Zhonghua nankexue ; Zhonghua nankexue;(12): 681-688, 2006.
Article de Chinois | WPRIM | ID: wpr-343547

RÉSUMÉ

<p><b>OBJECTIVE</b>To analyze the polymerase gamma(Polg) gene polymorphisms in Chinese idiopathic infertile males and evaluate the correlation of the polymorphisms with male infertility.</p><p><b>METHODS</b>We conducted a study of Polg CAG repeats in the sperm or blood DNA of 55 asthenospermia patients, 57 oligospermia patients, 34 azoospermia patients, and 104 controls with PCR and GeneScan. Phenotypic data were available in all the subjects, including semen parameters, and clinical profiles.</p><p><b>RESULTS</b>The frequency of 10/not 10 CAG genotype in asthenospermia patients was higher than in the other groups, but with no significance.</p><p><b>CONCLUSION</b>Our findings have shown for the first time that there exits an ethnic difference between Chinese and European males in the number of CAG repeats of Polg gene, and that 10/not 10 CAG genotype may affect sperm motility.</p>


Sujet(s)
Adulte , Humains , Mâle , Études cas-témoins , Chine , Épidémiologie , DNA Polymerase gamma , DNA-directed DNA polymerase , Génétique , Fréquence d'allèle , Génotype , Infertilité masculine , Épidémiologie , Ethnologie , Génétique , Réaction de polymérisation en chaîne , Répétitions de trinucléotides
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