W3D improves LPS-induced acute lung injury in mice by regulating TLR4/MD2-NF-κB p65 signaling pathway / 中国药理学通报

Aim To investigate the protective effect of new structure compound 4-(5'-dimethylamino)-naphthalenesulfonyl-2 (3H)-benzoxazolone (W3D) on lipopolysaccharide (LPS) induced acute lung injury (ALI) and the underlying mechanism. Methods ICR mice were randomly divided into control group, LPS model group, chlorzoxazone (12.5 mg · kg

Rapid characterization of chemical constituents and rat metabolites of Fufang Gancao Tablets by UHPLC-LTQ-Orbitrap mass spectrometer / 中国中药杂志

Ultra-high-performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPLC-HR-MSn ) method was used to analyze the constituents of Fufang Gancao Tablets and its main metabolites in rat plasma. Rat plasma was collected both before and after oral administration of Fufang Gancao Tablets. After solid phase extraction, ACQUITY UHPLC BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) was used with 0.1% formic acid-acetonitrile solution as the mobile phase for gradient elution. The chemical components in Fufang Gancao Tablets and their prototypes and metabolites in plasma samples were analyzed by LTQ-Orbitrap equipped with an ESI ion source in a positive ion mode. Based on the accurate mass measurements, the retention time and mass fragmentation patterns, a total of 55 compounds were tentatively identified from Fufang Gancao Tablets, including 42 flavonoids, 9 triterpenes and 4 alkaloids. Furthermore, metabolites in rat plasma after oral administration of Fufang Gancao Tablets were also analyzed. A total of 26 compounds were identified, including 20 prototypes and 6 metabolites mainly through metabolic pathways of hydroxylation, glucuronide conjugation, and sulfate conjugation, etc. Our results showed that the ultra-high-performance liquid chromatography coupled with linear ion trap quadrupole Orbitrap high resolution mass spectrometry (UHPLC-LTQ-Orbitrap) could comprehensively elucidate the chemical constituents of Fufang Gancao Tablets and their migrating components in rat plasma, providing scientific basis for further studying the metabolism process and pharmacodynamic substance of Fufang Gancao Tablets.

Cellphone electromagnetic radiation damages the testicular ultrastructure of male rats / 中华男科学杂志

<p><b>Objective</b>To investigate the influence of cellphone electromagnetic radiation (CER) on the testicular ultrastructure and the apoptosis of spermatogenic cells in male rats.atability, feasibility, applicability, and controllability in the construction of experimental animal models, we compared the major anatomic features of the penis of 20 adult beagle dogs with those of 10 adult men. Using microsurgical techniques, we performed cross-transplantation of the penis in the 20 (10 pairs) beagle dogs and observed the survival rate of the transplanted penises by FK506+MMF+MP immune induction. We compared the relevant indexes with those of the 10 cases of microsurgical replantation of the amputated penis.</p><p><b>METHODS</b>Thirty adult male SD rats were equally randomized into a 2 h CER, a 4 h CER, and a normal control group, the former two groups exposed to 30 days of 900 MHz CER for 2 and 4 hours a day, respectively, while the latter left untreated. Then the changes in the ultrastructure of the testis tissue were observed under the transmission electron microscope and the apoptosis of the spermatogenic cells was determined by TUNEL.</p><p><b>RESULTS</b>Compared with the normal controls, the rats of the 2 h CER group showed swollen basement membrane of seminiferous tubules, separated tight junction of Sertoli cells, increased cell intervals, apparent vacuoles and medullization in some mitochondria, and increased apoptosis of spermatogenic cells, mainly the apoptosis of primary spermatocytes (P<0.05 ). In comparison with the 2 h CER group, the animals of the 4 h CER group exhibited swollen basement membrane of seminiferous tubules, more separated tight junction of Sertoli cells, wider cell intervals, incomplete membrane of spermatogonial cells, fragments of cytoplasm, nuclear pyknosis and notch, slight dilation of perinuclear space, abnormalities of intracellular mitochondria with vacuoles, fuzzy structure, and fusion or disappearance of some cristae, and increased damage of mitochondria and apoptosis of spermatogenic cells, including the apoptosis of spermatogonial cells, primary spermatocytes, and secondary spermatocytes (P<0.05 ).</p><p><b>CONCLUSIONS</b>CER can damage the testicular ultrastructure and increase the apoptosis of spermatogenic cells of the male rat in a time-dependent manner, and the apoptosis of spermatogenic cells may be associated with the damage to mitochondria.</p>

In vitro effect of total flavones of Fructus Chorspondiatis on expression of collagen type I and type III mRNA and protein of cultured rat cardiac fibroblasts / 药学学报

This study aims to investigate the effect of total flavones of Fructus Chorspondiatis (TFFC) on the mRNA and protein expression of collagen type I and III of rat cardiac fibroblasts (CFs) induced by angiotensin II (Ang II), and explore its anti-myocardial fibrosis molecular mechanism. Neonatal rat CFs were prepared from Sprague-Dawley rats (1-3 d after birth). The expression of collagen type I and III mRNA and protein were measured by RT-PCR and Western blotting, respectively. The study showed that stimulation of neonatal rat CFs with 100 nmol.L-1 of Ang II for 72 h resulted in a significant increase of the expression of collagen type I and III mRNA and protein. The changes on the expression level were blocked by TFFC. The results demonstrated that TFFC can inhibit myocardial fibrosis induced by Ang II in rats, which is probably associated with the collagen type I and III mRNA and protein levels up-regulated by Ang II, and TFFC was shown to decrease the expression levels of collagen type I and III mRNA and protein.

Magnetic resonance imaging assessment of the lateral pterygoid muscle in Class III malocclusion subjects / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the relationship between Class III malocclusion and pathological changes in temporomandibular joint (TMJ) structures using magnetic resenonce imaging (MRI).</p><p><b>METHODS</b>Twenty-four Class III malocclusion adult patients and 10 normal control cases were included in the study. The characteristics of lateral pertygoid muscle (LPM) in the sample group and the control group were assessed.</p><p><b>RESULTS</b>More pathological changes of LPM were found in Class III malocclusion adult patients (36 TMJ). The changes included hypertrophy, atrophy and contracture. And there was no relation between the pathological changes of LPM and the symptom of temporomandibular disorders (TMD).</p><p><b>CONCLUSIONS</b>The frequency of pathological changes of LPM was greater in patients with Class III malocclusion than in the control group.</p>

A preliminary study on the occlusal contact changes during retention in adolescent patients / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the changes of the occlusal contact characteristics in adolescent patients during 12 months after active orthodontic treatment.</p><p><b>METHODS</b>Twenty adolescent patients with Hawley retainers after active orthodontic treatment were divided into none occlusal interference group and occlusal interference group. The occlusion of the patients was examined with T-Scan II system directly after the appliance removal (T1) and after an average retention period of 12 months (T2). The changes of occlusal contact characteristics were observed.</p><p><b>RESULTS</b>The disclusion time during protrusion, left and right lateral movements reduced significantly. The average disclusion time decreased [from (1.07 ± 0.87), (0.91 ± 0.47), (0.76 ± 0.43) s to (0.43 ± 0.25), (0.67 ± 0.41), (0.50 ± 0.27) s] significantly (P < 0.05). The occlusal interference disappeared in 4 patients and 1 patient with occlusal interference showed masticatory muscle symptom. The dynamic occlusion [from (1.25 ± 1.11), (0.84 ± 0.15), (0.52 ± 0.49) s to (0.35 ± 0.15), (0.36 ± 0.15), (0.33 ± 0.11) s] improved significantly (P < 0.05) in none occlusal interference group after retention and no statistical differences were found in the occlusal interference group (P > 0.05).</p><p><b>CONCLUSIONS</b>The overall dynamic occlusion improved after retention in patients with retainers. The presence of occlusal interference affected the self-improvement process and increased the chance of the disorders of stomatognathic system, such as mandibular abnormal movements. Therefore, functional occlusion evaluation and final detailing were needed before appliance removal.</p>

Ultrasonographic evaluation of tongue movement during swallowing in severe skeletal Class III malocclusion in adult patients / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the tongue movement during swallowing in skeletal Class III adult patients using M-mode ultrasonography combined with cushion scanning technique.</p><p><b>METHODS</b>Tongue movement during swallowing in 20 skeletal Class III adult patients (11 females, 9 males, mean age 20.5 years) and 11 healthy adults (8 females, 3 males, mean age 23.5 years) was examined with M-mode ultrasonography combined with cushion scanning technique. The results were compared.</p><p><b>RESULTS</b>Compared with mature swallowed, the skeletal Class III adult patients had longer phase 2 [(0.24 +/- 0.07) s], phase 3 [(1.17 +/- 0.21) s], longer total duration [(1.98 +/- 0.24) s], faster speed in phase 1 [(3.20 +/- 1.30) mm/s], and smaller range in phase 5 [(0.38 +/- 0.28) mm].</p><p><b>CONCLUSIONS</b>The skeletal Class III adult patients had distinctly different tongue movements during swallowing.</p>

Stability of the miniscrew implant during healing period / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the stability of mini-screw implant during different healing periods in the unloaded conditions.</p><p><b>METHODS</b>Sixty titanium mini-screws were used in this study. A total of 10 New Zealand white rabbits were randomly divided into 5 groups according to different healing periods. The stability was evaluated biomechanically at different time points. One-way ANOVA and correlation analyses were used.</p><p><b>RESULTS</b>(1) After 4 weeks and 8 weeks of healing, maximal pull-out loads were (179.68 +/- 24.65) N and (212.06 +/- 42.51) N, respectively. The maximal torques were (0.47 +/- 0.13) N.m and (0.61 +/- 0.16) N.m, respectively. (2) Significant difference in maximal torque was found between 4 week and 8 week healing groups. (3) Both reverse torque and pull-out load significantly correlated with healing time.</p><p><b>CONCLUSIONS</b>Fourth-week is a critical time point in the progress of osseointegration. Within 8 weeks of healing process, the stability of implant was significantly correlated with healing time.</p>