RÉSUMÉ
【Objective】 To investigate the differential diagnosis of 1 anti-C, e alloantibodies combined with anti-e, Jkb mimicking alloantibodies by absorption-elution test and titer integral method. 【Methods】 ABO, Rh and Kidd blood group antigens were identified by tube method. Two sets of panel cells were used for antibody screening and antibody specificity identification by saline method, polyamine method and microcolumn gel method.The antibody was further confirmed by multiple absorption-elution tests and titer integral method. RHCE and JK gene were sequenced by multiple PCR. 【Results】 Serological gene sequencing analysis showed that the ABO blood group of the patient was A type with Rh subtype ccDEE and was positive for direct antiglobulin test (DAT). Multiple absorption-elution tests and titer integral method demonstrated that the serum of the patient contained anti-C, e alloantibodies along with anti-e, Jkb mimicking autoantibodies and there were anti-e, Jkb mimicking autoantibodies on red blood cells(RBCs). According to gene sequencing analysis, there was G>C at exon 676 of the RHCE gene, and the remaining exons were not mutated, suggesting that the RHCE phenotype was ccEE. The 838 G/A heterozygote of exon 9 in JK gene, Jk blood group phenotype was Jk (a+ b+ ). Cross matched type A ccDEE and Jk(a+ b-) RBCs were transfused, and no adverse reactions occurred. 【Conclusion】 Serology combined with molecular biology to identify the phenotype of the patient′s RBCs, absorption-elution test and titer integral method to identify the antibody of the patient′s serum can detect the alloantibody type, thus providing strategies for targeted blood transfusion.
RÉSUMÉ
Establishment of an appraisal system in line with the Party building work of the new era, is imperative for implementation of the Party building responsibility system and furthering Party building quality. In September 2019, the authors customized a questionnaire and surveyed 629 in-job Party members of 10 tertiary public hospitals. By means of literature review, questionnaire surveys and expert interviews, they studied the current Party building appraisal system and existing problems. In addition, they probed into the significance of applying target management theory in the appraisal of Party building work at public hospitals, and applied such a theory in setting up a Party building appraisal system in line with the requirements of the Party in the new era, in such aspects as appraisal goal setting, management of the appraisal process, improvement of the appraisal system, and feedback of appraisal results.
RÉSUMÉ
Based on the study of theory research and teaching practice, Nanjing University of Aeronautics & Astronautics has tentatively put forward the concept of “teaching force”. At the same time, Nanjing University of Aeronautics&Astronautics has integrated various resources and systemat-ically constructed the teaching force cultivation mechanism focusing on “fanning out from point and line to area, two stage financing, internal and external drive”, which has effectively promoted the all-round development of young teachers in the teaching, personal, professional and organizational aspects.