Construction of a prognostic risk prediction model in liver cancer for macrophage related genes based on single-cell RNA-seq and bulk RNA-seq data / 实用肿瘤学杂志

Objective The aim of this study was to identify macrophage related genes(MRGs)in liver cancer and construct a prognostic risk prediction model for liver cancer.Methods The liver cancer scRNA-seq data from the GEO database were down-loaded to identify genes specifically expressed in macrophages as MRGs.The GO and KEGG functional enrichment analyses on MRGs were conducted.In the TCGA-LIHC dataset of the TCGA database,multiple random sampling single factor Cox regression for single-factor Cox regression,LASSO regression,and multivariate Cox regression analyses were employed to identify MRGs for liver cancer prognosis prediction,and a liver cancer prognostic prediction model was constructed.Results The results obtained 8 major cell types,including those containing macrophages through clustering using scRNA-seq data from the GEO database.The proportion of macrophages in the immune microenvironment of liver cancer was significantly higher than that of normal tissues(P=0.016),and genes such as SPP1,RNASE1,and MMP9 were highly expressed.Multiple metabolic pathways,including purine metabolism,citric acid cycle,and drug metabolism cytochrome P450 were activated in liver cancer-associated macrophages.This study identified 777 MRGs from liver cancer scRNA-seq(LogFC＞0.25,P＜0.05),which mainly involved in functions such as actin binding and regula-tion of immune receptor activity.Seven MRGs,including ATP1B3,ATP6V0B,HBEGF,KLF2,NR1H3,RAB10,and SPP1 were select-ed from the 169 stable prognostic genes(P＜0.05)for the construction of the prognosis model.The AUC values for the 1,3,and 5-year survival outcomes of the model in the TCGA liver cancer cohort were 0.791,0.791,and 0.751,respectively.In the validation ICGC cohort,they were 0.614,0.682,and 0.688,respectively,demonstrating good predictive performance.In liver cancer patients with high prognosis risk scores,the expression of macrophages M0,neutrophils,and regulatory T cells was higher(P＜0.05),and im-munosuppression and immune activation coexisted.Conclusion Liver cancer MRGs can serve as potential biomarkers for predicting the prognosis of liver cancer patients.These liver cancer MRGs are mainly associated with actin binding,immune receptor activity,and infiltration of various immune cells.

Factors influencing HIV testing and counselling services among men who have sex with men in Western China: a cross-sectional study based on Andersen's Behavioral Model

BACKGROUND@#Men who have sex with men (MSM), as a marginal population, has been largely ignored by health service projects. We assessed the utilization of HIV testing and counselling services and its influencing factors based on Andersen's Behavioral Model, so as to provide a theoretical basis for future infectious disease prevention and control strategies and health services policy formulation for these population.@*METHOD@#This was a cross-sectional study. A sample survey was conducted in Western China, and an anonymous self-administered questionnaire survey was conducted among MSM. Based on Andersen's Behavioral Model, the questionnaire divided the influencing factors into predisposing factor, enabling factor and need factor. Multivariate logistic regression analysis was used to explore the factors influencing the utilization of HIV testing and counselling.@*RESULTS@#There were 3184 valid questionnaires. In the survey of HIV health services, 82.85% MSM had HIV testing and 64.98% MSM had HIV counselling, respectively. Among the predisposing factor, age 25 years old and over was a facilitator of HIV testing and counselling, and ethnicity was a factor associated with HIV testing. Among the enabling factor, MSM living in urban were more likely to have access to testing and counselling services, and income was also linked to HIV testing. Among the need factor, a high level of HIV knowledge could promote testing and counselling, and a history of sexually transmitted diseases (STD) was a facilitator of testing.@*CONCLUSIONS@#HIV testing is widespread in Western China and higher than counselling service. MSM with high-risk characteristics should be identified as a priority in the future public health services.

Exploration on postgraduate education and talent training for Master of Applied Statistics in medical colleges / 中华医学教育探索杂志

In medical field, the demand of high-level applied statistical talents is becoming more and more urgent, and the number of medical colleges to train graduate students of Master of Applied Statistics (MAS) is increasing gradually. Taking Chongqing Medical University as an example, this article introduces the objective, orientation and training mode of MAS education, analyzes the main characteristics of MAS professional education and talent training, discusses the problems mainly on training system and teaching base, combined with the interview results of MAS graduate students studying in CMU, and puts forward target suggestions including expanding the enrollment scale, optimizing the "two-tutorial system", advancing the training system and developing new teaching bases, to provide reference and experience for the teaching and training of MAS graduate students in medical colleges.

Factors influencing protective behavior in the post-COVID-19 period in China: a cross-sectional study

BACKGROUND@#The study aimed to explore the factors influencing protective behavior and its association with factors during the post-COVID-19 period in China based on the risk perception emotion model and the protective action decision model (PADM).@*METHODS@#A total of 2830 valid questionnaires were collected as data for empirical analysis via network sampling in China. Structural equation modeling (SEM) was performed to explore the relationships between the latent variables.@*RESULTS@#SEM indicated that social emotion significantly positively affected protective behavior and intention. Protective behavioral intention had significant direct effects on protective behavior, and the direct effects were also the largest. Government trust did not have a significant effect on protective behavior but did have a significant indirect effect. Moreover, it was found that government trust had the greatest direct effect on social emotion. In addition, we found that excessive risk perception level may directly reduce people's intention and frequency of engaging in protective behavior, which was not conducive to positive, protective behavior.@*CONCLUSION@#In the post-COVID-19 period, theoretical framework constructed in this study can be used to evaluate people's protective behavior. The government should strengthen its information-sharing and interaction with the public, enhance people's trust in the government, create a positive social mood, appropriately regulate people's risk perception, and, finally, maintain a positive attitude and intent of protection.

Seroepidemiology of Epstein Barr virus in men who have sex with men in Chongqing area / 中国感染与化疗杂志

Objective To investigate the seroepidemiology of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV) in adult men who have sex with men (MSM) in Chongqing area. Methods Nonprobability sampling method was used to test EB-CA-IgG, EB-NA-IgG and EB-VCA-IgM in the sera of 1082 MSMs from the clinical trials of HIV/AIDS treatments in Chongqing area from 2012 to 2015, and 1059 healthy individuals by means of enzyme-linked immunosorbent assay. The results were analyzed by Chi-square test. The difference was considered statistically significant when P<0.05. Results The 1082 MSM included 130 HIV positive and 952 HIV negative subjects. The prevalence of prior EBV infection was 92.6% in total MSM population, 88.5% in HIV-positive MSM, and 93.2% in HIV-negative MSM. The prevalence in total MSM and HIV negative MSM was significantly higher than that in control group (89.9%). Prior EBV infection was not?found?in?0.5%?of?the?total?MSM,?0.8%?of?HIV?positive?MSM?and?0.4%?of?HIV?negative?MSM,?all?significantly?lower?than?that?of control group (5.0%) (P<0.05).?Finally,?the?rate?of?EBV?reactivation?in?HIV?positive?MSM?(10.0%)?was?significantly?higher?than?that in control group (3.8%) and in HIV negative MSM group(4.1%) (P<0.005). Conclusions EBV infection is highly prevalent in MSM, higher than that in the general population. The rate of EBV reactivation in HIV negative MSM is similar to that in general population. The rate of seroepidemiology-based EBV reactivation is significantly higher in HIV positive MSM, which may be associated with the immunocompromised status post HIV infection.

Efficacy of human immunoglobulin in treating elderly head and facial herpes zoster and effect on peripheral blood TNF-α / 重庆医学

Objective To observe the effect of human immunoglobulin on elderly head and facial post herpetic neuralgia (PHN) and peripheral blood TNF-α.Methods One hundred and twenty-two inpatients with PHN aged ≥65 years old were selected and divided into the observation group (52 cases) and control group (70 cases) by the systematic sampling method.The control group was given the early conventional combined therapy,while on this basis the observation group was intravenously dripped by human immunoglobulin.The incidence rate of PHN and pain visual analog scale(VAS) score at 1,2,3 months after recovery discharge from hospital were recorded in the two groups.Other 20 healthy elderly people were selected as the healthy control group.The TNF-α level was determined in the two patients groups before and after treatment and in the healthy control group.Results The PHN occurrence rate at 1,2,3 months after discharge in the observation group was significantly lower than that in the control group(P＜0.05);the VAS score after treatment and at 1,2,3 months after discharge in the observation group was significantly lower than that in the control group (P＜0.05);serum TNF-α level after treatment in the observation group was significantly lower than that in the control group (P＜0.01),moreover which was close to the level in the healthy control group(P＞0.5).Conclusion Human immunoglobulin can reduce the PHN occurrence in the old people with head and facial herpes zoster and reduce the peripheral blood TNF-α level.

The effect of the allocation of health resources on doctor-patient relationship in Chongqing / 重庆医学

Objective To analysis the basic condition of the health resources in Chongqing ,and to explore the effect of the al‐location of health resources on doctor‐patient relationship .Methods Health statistics yearbook of Chongqing city was collected ,the fairness of the allocation of health resources in chongqing carried on the analysis and evaluation ,using the DEA model to analyze and evaluation the efficiency of the allocation of health resources in chongqing ,self‐designed questionnaire was used;hospitals ,doc‐tors and patients were investigated ,the descriptive and statistical analysis carried on .Results The distribution of health resources in Chongqing was inequitable on a regional .This inequitable distribution had been evaluated to the effect that ,as per the DEA mod‐el ,7 prefectures were effective with another 5 slightly effective and 26 as DEA ineffective .In the 8 hospitals that had been investiga‐ted ,the occurrence of medical disputes was undergoing a surge ,which indicates the tense relation between doctors and patients .Con‐clusion The total amount of health resources in Chongqing is fairly insufficient ,the high quality health resources are scarce .Unbal‐anced allocation of health resources and allocation inefficiency is the main reason for nervous doctor‐patient relationship and contra‐diction focus .

Reform and practice on teaching of medical statistics course based on web computing platform / 中华医学教育探索杂志

Improving medical students' calculation ability in statistics has become the focus and difficulty of medical statistics course teaching, and its application relies heavily on statistical calculation software. In order to explore a new teaching approach which combined the advantages of traditional method and web-based calculation, we intended to build a web computing platform applying the Browser/Server (B/S) mode based on the campus network, and to revise the current syllabus of medical statistics, as well as to create a virtual web-lab containing a typical case library. Practice has proved that the new mode effec-tively improved the practice capability of students and changed the traditional teacher-centered teaching approach.

Demand situation and master degree of the medical correlation personnel on medical statistics methods / 中华医学教育探索杂志

Objective The aim of this study was to assess the current situation and master degree on medical statistics among the medical correlation personnel, in order to provide useful information for transformation of education towards the medical correlation personnel . Methods Using cluster random sampling method to select 358 the medical correlation personnel who were recruited from health institutions in Chongqing to respond to the questionnaires. Questionnaire including respondent basic information, statis-tical knowledge and statistical knowledge needs. The data of the survey were analyzed by chi-square test, descriptive statistics analysis. Results 94.7% of the surveyed used statistical knowledge in the process of work or study. The main concentration of medical statistical knowledge utilization rate, the proportion of receive professional education or training courses of medical statistics and the willingness to participate in specialized training of medical statistics is 20-30 age group, No Title group, Master and over group andStudents group. As for ten categories of common statistical methods, thirty percent of people can use them skillfully, but the majority of the more advanced statistical analysis methods cannot be used or have never heard of. The greatest demand for learning ten kinds of common statistical methods is calculating cor-rectly through statistical software. Conclusion The vast majority of medical personnel need to use medical statistics in the actual work and study, but they have a lower mastery of statistical methods. Therefore, we should strengthen the medical statistics teaching and training for medical personnel.

Publication impact of the Chinese Journal of Hepatology from 2006 to 2014 / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To analyze and evaluate the publication impact of the "Chinese Journal of Hepatology" from 2006 to 2014 by assessing academic standards, disciplinary status, influence, communication range, reader usage and reader awareness.</p><p><b>METHODS</b>The WanFang database was searched and 108 bibliometric indicator statistics were retrieved for the "Chinese Journal of Hepatology", including each issue's papers amount, articles types, diseases, funded projects, references, authors, units, geographical distribution, and publication cycle.</p><p><b>RESULTS</b>From 2006 to 2014, the "Journal of Hepatology" published a total of 2 536 articles, with these articles being cited 7 763 times. The values for impact factor, total cites, references to periodicals, citation rate, immediacy index, citation half-life, and funded papers were found to be better than the average level of other domestic journals.</p><p><b>CONCLUSION</b>In recent years, the "Chinese Journal of Hepatology" has gained the attention of both readers and authors, and has improved its academic impact.</p>

Fairness analysis of human resource allocation in medical health industry of Chongqing from 1997 to 2012 / 重庆医学

Objective To analyze the equity of the allocation of the health human resources in Chongqing from 1997 to 2012 , and to provide the basic information for regional health planning .Methods The statistical description ,Gini Coefficient and Theil in-dex are used to analyze the previous allocation and trends of the health human resources and the equity in Chongqing during the 16 years based on the distribution of demography and geography .Results The number of the health human resources grew rapidly from 1997 to 2012 .The Gini coefficients of health professionals and medical practitioners (and assistants) are under 0 .3 based on the distribution of demography ,while registered nurses′are fluctuation in 0 .4 .However ,from the distribution of geography ,the Gi-ni coefficient of health professionals ,medical practitioners (and assistants) and registered nurses are above 0 .5 .The Gini coefficient of the one hour economic circle is higher than two wings areas .The trends about the totle Theil index are consistent with the Gini coefficient .Based on the distribution of demography ,the Theil index between regions is higher than the one within the region .On the contrary ,the Theil index between regions is below the one within the region by the geographic distribution .Conclusion Com-pared to the national ,less total health human resources are reserved in Chongqing .Distribution by demographics about health human resources are more equitably than the one by geographic distribution .The variances between the economic circles contribute more to the total Theil index than those within the economic circles .The government′s leading role should be strengthen ,and enhancing the regional health planning .

Design and research of medical statistics question database management system / 中华医学教育探索杂志

This thesis mainly expounds the design ideas and the realization methods of medical statistics question database management system.Specifically speaking,the function modules,data structure and business processes of the system are designed based on the analysis of questions,test papers and the needs of system functions.We choose Visual Basic 6.0 to develop the system interface,establish the question database with Access 2003,and make use of Word 2003 to output documents.This system can achieve the separation of teaching and testing effectively which can make examinations more normal and scientific.

Reforms for the curriculum system of preventive medicine / 中华医学教育探索杂志

Against the deficiencies of the current preventive medicine education curriculum,training programs and curriculum were reformed according to the training objectives of the modem preventive medicine, while new ideas of preventive medical personnel training were explored in order to cultivate practical professionals with a certain ability to innovate and to adapt the development of modern public health and preventive medicine preferably.

Exploration on Setting up an Evaluation Norm System by Students for College Textbooks / 中华医学教育探索杂志

A comprehensive evaluation on textbooks depends not only on experts' opinions but also on the first-hand feedback information from users.This research,based on the Evaluation Norm System issued by Education Department and the real situation of users(students),has set up an evaluation norm system by students for college textbooks.Through the test of this system,it is thought that it can reflect a basic picture of textbooks and is easy to be operated.

Survey on cognition of occupational blood exposure among nursing staff in Chongqing / 第三军医大学学报

Objective To understand the cognition status about preventing from blood pathogens in Chongqing nursing staff of occupational exposure to blood and to provide the evidence necessary to the education of occupational prevention.Methods Totally 1 270 nurses in 20 hospitals in Chongqing were investigated by self-designed Questionnaires.The contents included understanding,acquisition approaches,the knowledge that should be known etc..Results The knowledge of occupational exposure was inadequate.The proper answers averaged 54.59% in questionaire and there was significant difference in the cognition about occupational blood exposure among those of different education background,professional title,nursing age and types of employment(P

The Analysis of the Factors Affecting the Employment of Grade 2006 Undergraduates in a Medical University / 中华医学教育探索杂志

Goal To research the major factors affecting medical undergraduates' employment.Method In combination with quantity and quality,we reasonably analyzed the factors affecting the employment of Grade 2006 undergraduates in a Medical University in Chongqing.Result The major factors consist of the inadequate knowledge、the increased condition admission、hidden rule and the unreasonable objects.Conclusion Medical universities should try their best to adapt to the development of the society and medical students should try to become the useful talents.

New idea about the teaching of medical statistics for medical postgraduate students / 中华医学教育探索杂志

Objective To find out a new teaching pattern of Medical Statistics for medical postgraduate students.Methods To manifest the longitudinal thought of the medical research process from the teaching idea,the teaching content arrangement and the teaching method utilization.Results To discuss the superiority of the new pattern which provides the content frame and the teaching method based on the integrating theory and the practice.Conclusion The teaching reformation of Medical Statistics must be advantageous in improving the learner’s power of the medical scientific research,including selection of the scientific research topic,application of project,the quality control of the project,analysis of the material and inference of the conclusion,paper writing,self-appraisement and achievement evaluation.