RÉSUMÉ
Objective:To construct a comprehensive evaluation index system for integrated health management service capacity of the close-knit medical consortium,and to provide an effective evaluation tool for promoting the construction of a close-knit medical consortium in China.Methods:Based on service chain theory,the initial index pool is established from four dimensions of service guarantee,service guidance,service provision and service evaluation through policy analysis,literature reading and field investigation.Delphi method is used to improve the evaluation indicators through two rounds of expert consultation,and the analytic hierarchy process is used to determine the weight of each indicator.Results:The constructed index system consists of 4 first-class indicators,11 second-class indicators and 50 third-class indicators.The weights of service guarantee,service guidance,service provision and service evaluation are 0.133,0.301,0.401 and 0.165 respectively.Conclusions:Based on the service chain theory,this study applies Delphi expert consultation method and the analytic hierarchy process and invites experts in relevant fields to carry out consultation,aiming at constructing a comprehensive evaluation index system for the integrated health management service capacity of the close-knit medical consortium with strong operability and scientific rationality,and providing an effective evaluation tool for promoting the construction of the close-knit medical consortium in China.
RÉSUMÉ
Objective To investigate the effects of regular and irregular mandatory treadmill running (TR) on learning and memory abilities,stress response,survival and maturation of newborn neurons in dentate area of C57 mice. Methods Forty-eight male C57BL/6 mice were randomly divided into regular running group (n=16,definite time and quantity), irregular running group (n=16,definite time and different quantities) and sedentary group (n=16).Injection of bromodeoxyuridine (BrdU,50 mg/kg) was given intraperitoneally to mice from the 1st d to the 7th d of exercise,and then,these mice were subjected to a 4-wk TR training course or sedentary exercise.Double irnmunofluorescence labeling was employed to observe the effect of TR on survival and maturation of newborn neurons. Mice were trained on the Morris water maze to test the abilities of learning and memory. Corticosterone (CORT).level in the venous blood was measured with a radioimmunoassay kit. Results As compared with that in mice of the irregular running group and sedentary group,significant decrease of latency was noted in mice of the regular running group on the 1st, 3rd, 4th and 5th d of place navigation test (P＜0.05). As compared with that in mice of the sedentary group, significant decrease of latency was noted in mice of the irregular running group and regular running group on the 2nd d of place navigation test (P＜0.05),while no significant difference of that was noted between mice of the irregular running group and regular running group (P＞0.05).The platform was removed for a 60-s probe test 4 h after the last trial to test the recall ability, and the times of staying in the target zone in each group were regular running group＞irregular running group＞control group, and significant difference was noted between each 2 groups (P＜0.05). The numbers of double immunofluorescence labeled cells in the dentate gyrus were counted as regular running group＞irregular running group＞control group, and significant difference was noted between each 2 groups (P＜0.05). The serum CORT level was measured as regular running group＜irregular running group＜control group, and significant difference was noted between each 2 groups (P＜0.05). Conclusion Mandatory treadmill running could improve the learning and memory abilities,which may be related to the increment of survival and maturation of neural precursor cells,and decreased level of serum CORT; and the effect of regular mandatory treadmill running is better than that of irregular mandatory treadmill running.