Clinical efficacy of decitabine combined with half dose CAG regimen in treatment of patients with relapsed/refractory acute myeloid leukemia / 白血病·淋巴瘤

Objective To observe the clinical efficacy of decitabine combined with half dose CAG regimen in patients with relapsed/refractory acute myeloid leukemia (AML).Methods Clinical data of 8 patients with relapsed/refractory AML who took the therapeutic regimen with decitabine combined with half dose CAG regimen in the Third People's Hospital of Datong from January 2015 to January 2017 were collected.The clinical efficacy and adverse effects were observed.Results After one course treatment by decitabine combined with half dose CAG regimen,3 patients achieved complete remission,and 2 patients achieved partial remission.The major adverse reactions were myelosuppression and infection,8 patients appeared grade Ⅲ-Ⅳ myelosuppression,5 patients appeared infection,2 patients appeared grade Ⅰ abnormality of liver and no treatment-related death occurred.Conclusion Decitabine combined with half dose CAG regimen is effective for relapsed/refractory AML,and this regimen is worthy of further study.

Study on the effect of psychological intervention combined with preoperative chemotherapy with gefitinib on elderly patients with non- small cell lung cancer after localized resection of lung cancer / 中国生化药物杂志

Objective To investigate the clinical efficacy of psychological intervention and gefitinib in the treatment of elderly patients with non-small cell lung cancer with limited pulmonary resection. Methods The control group were all treated with limited pulmonary resection. Preoperative chemotherapy was given to gefitinib. The study group was given psychological intervention during the preoperative chemotherapy on the basis of the control group. Results The resection rate in the study group was 93.55％, while in the control group was 74.19％, the difference in the two groups was statistically significant(P<0.05). The transfer rate was 3.23％ and the recurrence rate was 3.23％ in the control group, which was significantly lower than those in the control group the transfer rate of 19.35％, the recurrence rate of 16.13％. 1 years survival rate in the study group 80.65％ was significantly higher than that in the control group 64.52％, the difference was statistically significant(P<0.05). Conclusion On the basis of routine preoperative application of gefitinib combined with local excision treatment, the elderly patients with non-small cell lung cancer with psychological intervention measures can make them achieve more ideal effect and prognosis.

Analysis of common gene mutations and cytogenetics in core binding factor related acute myeloid leukemia / 白血病·淋巴瘤

Objective To assess the prevalence of c-Kit and FLT3 gene mutations in core binding factor related acute myeloid leukemia (CBF-AML) and analyze the karyotype characteristics of the CBF-AML patients. Methods Mutations of c-Kit, FLT3-ITD and FLT3-TKD were detected by genomic DNA PCR and sequencing, and the karyotype changes were analyzed in 48 newly diagnosed CBF-AML patients. Results c-Kit aberrations were detected in 13(27.1 %) out of 48 patients, including 5 cases with exon 8 mutation and 8 cases with exon 17 mutation. c-Kit was more prominent in t(8;21) AML patients than in inv(16) AML patients [(33.3 %(9/27) vs 19.0 %(4/21), P<0.05]. Only 1 case (2.1 %) had FLT3-ITD mutation (FLT3-ITD+) and 3 cases (6.3 %) had FLT3-TKD mutation (FLT3-TKD +). Prevalence of RUNX1-RUNX1T1 with additional chromosome abnormality was as high as 25.9 %(7/27), in which sex chromosome elimination was the most common one, while prevalence of CBFβ-MYH11 with additional chromosome abnormality was low. Conclusion c-Kit gene mutations and RUNX1-RUNX1T1 additional chromosome abnormalities are common in patients with CBF-AML and would be helpful for individualized treatment studies.

Expression and significance of FasL and FAP-1 in acute myeloid leukemia / 白血病·淋巴瘤

Objective To explore the expression of the mRNA level of Fas (CD95) ligand/FasL and Fas-associated phosphatase-1/FAP-1 in acute myeloid leukemia. Methods The expression of FasL and FAP-1 were detected in 54 patients with AML and 10 normal subjects by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). β-actin used as internal reference. The changes of FasL was observed after induction chemotherapy in 16 AML patients. The expression of Fas was detected in 54 patients with AML by flow cytometry. Results The mRNA levels of FasL and in 54 patients were remarkably higher(P 0.05). The mRNA levels of FAP-1 in 54 patients was remarkly higher than that of the normal control. 8/29 cases in Fas-positive group were positive for FAP-1 mRNA expression. 19/25 cases in the Fas-refractory group didn't express FAP-1 mRNA. Conclusion The expression of FasL was high in AML. The rates of complete remission were high in FasL positive cases. The FasL level declined in patients with good response to therapy. The expression of FAP-1was partly expressed in AML. The expression of FAP-1 was less in Fas positive group.

Multicenter controlled randomized clinical trial of akatinol memantine for treatment of Aizheimer's disease / 中华神经科杂志

Objective To evaluate the clinical efficacy and safety of akatinol memantine in the treatment of Alzheimer's disease (AD).Methods Two hundred and forty-one patients with AD were randomly assigned to receive 10 mg of donepezil daily or 20 mg of memantine daily for 24 weeks.The primary efficacy variables were the Clinician' s Interview-Based Impression of Change Plus (CIBIC-Plus),the Alzheimer Disease Assessment Scale-cognition (ADAS-cog) and the Activities of Daily Living (ADL).The secondary efficacy variables were the Neuropsychiatric Inventory (NPI) and the Mini-Mental Status Examination (MMSE).Results Two hundred and seven patients completed the study and were evaluated at week 24.Both memantine and donepezil had significant efficacies at the end point, according to the ADAS-cog, the ADL, the NPI and the MMSE.Patients receiving memantine had a similar outcome as those receiving donepezil, according to the results of all the variables changes (CIBIC-Plus: memantine 3.4±0.8vs donepezil 3.5±0.8; ADAS-cog: memantine-4.7±5.8 vs donepezil-4.6±6.5; ADL: memantine -2.4±6.7 vs donepezil-2.2±5.3 ; NP1: memantine-5.8±9.0 vs donepezil-3.1±8.5 ; MMSE:memantine 1.7±3.1 vs donepezil 1.8±2.8, all P >0.05).The adverse events were as following: donepezil group 41.88% and memanintine group 30.58%.Conclusion The memantine as a new drug for AD, has the similar efficacy as donepezil, and it is safe.

The relationship of MRI features and clinical characteristics of subcortical ischemic vascular disease with cognitive impairment / 中华老年医学杂志

Objective To investigate the relationship of lacunar infarction (LI) and white matter lesion with cognitive impairment in patients with subcortical ischemic vascular disease (SIVD) Methods Fifty-three patients were diagnosed as SIVD according to the criteria of Erkinjuntti. The symptoms and signs were recorded by an interview and examination, and neuropsychological assessment and magnetic resonance imaging (MRI) were performed. A semi-automated MRI quantitative method was used to measure the volume of white matter hyperintensity (WMH) and the number of LI was counted. Correlation and the partial correlation analysis were performed to examine the relationship of general cognitive function with the volume of WMH and the number of LI. Results The pseudobulbar paralysis symptom and the upper motoneuron injury sign were the most common in these patients (18.9% and 37.7%). Correlation analysis showed that there was positive correlation between the volume of WMH and age (r = 0. 518, P < 0. 05), and there was negative correlcction between mini-metal state examination (MMSE) scures and the volume of WMH (r=-0.514, P<0. 05), After controlling confounding factors, only age was positively correlated with the volume of WMH (r=0. 400, P=0. 004). There were negative correlation between the number of LI, the volume of WMH and MMSE scores(r=-0. 456,-0. 514,-0. 385,-0. 382;all P<0. 05), and the years of education was positively correlated with MMSE scores (r= 0. 518, P< 0. 001). Conclusions Age may not be the main risk factor for cognitive impairment in patients with SIVD. The volume of WMH and the number of LI are independent risk factors for cognitive impairment in patients with SIVD. Patients with severer SIVD or more LI show poorer performance on cognitive function.

A primary study on the indexes change of coagulation and fibrinolysis in APL with ATRA and As2O3 / 肿瘤研究与临床

Objective To explore the indexes change of coagulation and fibrinolysis in APL with ATRA and As2O3.Methods Treatment group 18 eases were undeaaked ATRA 20-30 mg/d until relieved completely.0.1%arsenic trioxide 10 ml with 5%glucose 500 ml for 28 days.Normal control 10 cases were undertaked ATRA 45～60 mg/d until relieved completely.Results The CR rate of treatment group was higher than control group(P＜0.05),the patients of control group showed different reactions such as thirsty mouth,rationale acid syndrome.2 cases reacted strongly and the treatment had to be interrupted,the patients of treatment group had little reaction,but liver function were badly hurt and liver-protection treatment was needed.Fbg,D-dimer recovery time is 7 days,21 days in treatment group,14 days,21 days in control group.Conclusion CR rate is high and has little side effect,and indexes change of coagulation and fibrinolysis were recovered quickly,it has great significance to take D-dimer as early diagnose and observe the indexes change during the treatment.

Level of Fasting Plasma Glucose, True Insulin, C-peptide and Amyloid Beta-protein in Patients with Alzheimer Disease / 中国康复理论与实践

@#Objective To investigate the level of insulin and amyloid beta-protein (Aβ) in patients with Alzheimer disease(AD). Methods The levels of fasting plasma glucose, true insulin, C-peptide, Aβ40 and Aβ42 in 70 healthy age-matched adults and 55 patients with AD were measured and analyzed. Results No differences were observed for the levels of fasting plasma glucose between AD groups and normal group (P>0.05). The level of the true insulin and C-peptide of the moderate/severe AD group were significantly higher than that of the normal control group(P<0.05), as well aw that of the Aβ40 (P<0.05). The level of Aβ42 of the mild AD group waw significantly higher than that of the normal control group(P<0.01). Conclusion Plasma insulin and Aβ are abnormal in AD, and they are related to severity of dementia.

Value of 99Tcm-HL91 hypoxia imaging in identifying ischemic and hypoxic cells in law perfusion cerebral area / 中国组织工程研究

BACKGROUND: In the ischemic cerebral area there are viable tissues of low blood perfusion. Theses tissues can be rescued as long as blood supply is restored timely. As an imaging agent,99Tcm-HL91 can be used to demonstrate the viable cerebral tissues in hypoxic and ischemic area.OBJECTIVE: To investigate the value of 99Tcm-HL91 imaging in assessing ischemic stroke,in order to provide evidences for early intervention.DESIGN: An observational and controlled trial based on patients and healthy voluinteers.SETTING: A nuclear medicine department in a university and a neurological department and a nuclear medicine department in a provincial hospital.PARTICIPANTS: From March 2000 to September 2001,eighteen inpatients and outpatients suffering from ischemic cerebral diseases and 4 volunteers were enrolled in the study.METHODS: In the 18 patients,11 were clinically diagnosed as cerebral infarction,5 TIA,2 vertebrobasilar insufficiency. All the subjects underwent 99Tcm-HL91 cerebral hypoxia tomographic imaging. The labeled 99Tcm-HL91(555-1 110 MBq) was intravenously injected into the human body and imaging was conducted after 20 -30 minutes,17 patients underwent CT or MRI examination at the same time. Eleven patients underwent 99Tcm-ECD tomopraphic imaging on the next day. The results of the 3 examinations were compared with each other. The uptake in the inside and around-infarct areas was determined by direct visual observation on the images. Positive change was given by presence of increased uptake and negative by absence. The uptake change that could not be clearly defined as positive or negative was considered critical level. But the uptake on both hemispheres was compared with ROI technology. The change over 25% between hemispheres was set as positive and the other was negative. So there was no critical group in this study.MAIN OUTCOME MEASURES: The results of cerebral hypoxia imaging,CT,MRI and cerebral perfusion imaging.RESULTS: Among the 18 patients,5 were positive for hypoxia imaging(4infarction and 1 vertebrobasilar insufficiency). In the 99Tcm-ECD perfusion,6out of 11 patients manifested of regional decreased blood perfusion. Nine patients manifested of abnormal CT or MRI results. No abnormalities were detected in the 4 volunteers.CONCLUSION: Although 99Tcm-HL91 imaging for diagnosis of cerebral ischemic disease is affected by many factors,it can identify whether the ischemic tissue is hypoxic or necrotic when the perfusion imaging demonstrate low flow. So it helps in guiding rehabilitation intervention and foretelling prognosis.

Study on the modification surface material of heparinized polyurethane / 生物医学工程学杂志

In this study cationic and heparinized polyurethanes (PUs) were synthesized by a two-step solution polymerized method. Cationic and heparinized PUs were investigated by infrared spectroscopy, electron spectroscopy for chemical analysis (ESCA) and turbidity method. At the same time, the PUs proved of good biocompatibility through the laboratory tests, including blood coagulation time (CT), activated partial thromb plastin time (APTT) and fibroblast culture. These materials have good biocompatible function.

Applications of chitosan and its derivatives in enzyme immobilization / 中国海洋药物

The recent applications of chitosan and its derivatives in enzyme immobilization are reviewed in this paper. It is introduced that the immobilized enzyme on different kinds of chitosan and its derivitaves have been prepared by various immobilization methods. Their applications are also discussed. The chitosan and its derivatives are admirable carriers for enzyme immobilization with many advantages, such as various and simple immobilized methods? good biocompatibility and abundant resources.