Influence of different interventional opportunity on advanced aged patients with acute coronary syn‐drome non‐ST elevation type／ / 心血管康复医学杂志

To study influence of different interventional opportunity on advanced aged patients with acute coronary syndrome non‐ST elevation type (ACS‐NSTE).Methods : The180 advanced aged ACS‐NSTE patients from our hospital , were randomly and equally divided into group A , B and C , and they received interventional surgery within 1d ,2～3d and 3d later after hospitalization respectively .Serum levels of cystatin C (CysC ) , lipoprotein a [Lp (a)] , ApoA , hsCRP and vascular endothelial function indexes before and 7d after intervention , cardiac func‐tion indexes before and two months after intervention , and incidence of major adverse cardiovascular events (MACE) within two years after intervention were observed and compared among three groups .Results : Compared with group A and C on 7d after intervention , there were significant reductions in levels of CysC [ (0. 97 ± 0.13) mg／L vs .(1.08 ± 0.15) mg／L vs.(0.90 ± 0.11) mg／L] , Lp (a) [ (175.74 ± 18.18) mg／L vs.(197.89 ± 20. 15) mg／L vs .(163. 98 ± 16.54) mg／L] , hsCRP [ (1. 44 ± 0.20) mg／L vs.(2.34 ± 0.25) mg／L vs.(0.79 ± 0.09) mg／L] , plasma von Willebrand factor [vWF , (158. 79 ± 16.28)% vs.(174.68 ± 18. 28)% vs .(142.87 ± 14. 31)%] and endothelin‐1 [ET‐1 , (93. 28 ± 9.34) ng／L vs.(99.10 ± 9.94) ng／L vs .(86.43 ± 8.71) ng／L] , and significant rise in levels of ApoA [ (0.91 ± 0. 16) mg／L vs.(0. 82 ± 0.14) mg／L vs.(1.03 ± 0.17) mg／L] and NO [ (59. 92 ± 5. 93) mol／L vs.(55. 33 ± 5.48) mol／L vs.(64. 29 ± 6. 42) mol／L] in group B , P＜0. 01 all.After two months , com‐pared with group A and C , there were significant reductions in LVEDd [ (47.87 ± 4.72) mm vs.(50.58 ± 5.11) mm vs.(44. 68 ± 4. 50) mm] and LVESd [ (37.87 ± 3.82) mm vs .(40.57 ± 4. 19) mm vs.(34.98 ± 3. 52) mm] , and significant rise in LVEF [ (52. 87 ± 5.38)% vs.(49.04 ± 5. 02)% vs.(55. 89 ± 5.62)%] in group B , P＜0. 01 all.On two years after intervention , incidence rate of MACE in group B was significantly lower than those of group A and C (3.33% vs.15. 00% vs .31.67%, P＜0.05 or ＜0.01).Conclusion : Interventional therapy on 2～3d after hospitalization can significantly reduce inflammatory factor level , improve vascular endothelial function and cardiac function , and reduce incidence rate of MACE in ACS‐NSTE patients .

Research advances in biodegradable intervertebral fusion cage for spine / 局解手术学杂志

Objective At present, the mainstream surgical mode and the gold standard for the treatment of spine related diseases are still the intervertebral fusion with the intervertebral implantation of the intervertebral fusion device.The intervertebral fusion device routinely used in clinical practice cannot degrade in vivo after implantation, resulting in lifelong foreign body.High elastic modulus also leads to osteoporosis in adjacent vertebral bodies, which leads to implant sinking, stress shielding and'pseudomorphism'of fusion.The ideal biodegradable synthetic intervertebral fusion cage can be progressively degraded and eventually replaced by new bone, which has the advantages of elastic modulus close to cortical bone, good biocompatibility, X-ray permeability and good initial mechanical strength.In this paper, animal analysis of biodegradable intervertebral fusion cage, clinical effect analysis, current shortcomings and future trends were reviewed.

Detection of an NA gene molecular marker in H7N9 subtype avian influenza viruses by pyrosequencing / 病毒学报

This study aimed to establish a method for the detection and identification of H7N9 avian influenza viruses based on the NA gene by pyrosequencing. According to the published NA gene sequences of the avian influenza A (H7N9) virus, a 15-nt deletion was found in the NA gene of H7N9 avian influenza viruses. The 15-nt deletion of the NA gene was targeted as the molecular marker for the rapid detection and identification of H7N9 avian influenza viruses by pyrosequencing. Three H7N9 avian influenza virus isolates underwent pyrosequencing using the same assay, and were proven to have the same 15-nt deletion. Pyrosequencing technology based on the NA gene molecular marker can be used to identify H7N9 avian influenza viruses.

Effect of ursolic acid on retinal vascular form in oxygen-induced mouse retinopathy / 中华实用儿科临床杂志

Objective To investigate the effect of ursolic acid (UA) on retinal vascular form in oxygen-induced mouse retinopathy.Methods The 60 clean 7-day C57BL/6J mice were divided into 6 groups randomly:blank control group,model control group (PBS),positive control group (triamcinolone),UA intervention group (low dose,meta dose,high dose),with 10 mice in each group(right eye as experimental subject,10 eyes each group).The blank control group mice were raised in air,with other groups of mice in (750 ± 20) mL/L O2 high-oxygen environment for 5 consecutive days.The model control group mice and breastfeeding mice were put back in air enviroment(210 mL/L O2) on the 12th day after birth to induce the generation of retinal neovascufarization.The drug treatment was applied to the corresponding groups immediately when models were successful:3 μL sterile PBS was injected in model control group,3 μL 1.5,3.0 and 6.0 μg UA in UA intervention group,3 μL triamcinolone(1 mL ∶ 40 mg) in positive control group.All mice were killed after overdose of anesthesia on the 17th day,and their eyeballs were made into retinal tissue sections,HE dying,counting the neovasculanzation endothelial nuclei number which broke the retinal internal limiting membrane.The morphologic changes of retinal vessels were estimated by observing the vascular pattern with the technology of stretched preparation of retina.Results From the observation of tissue pathology slice in blank control group,the structure of internal limiting membrane was even,and the vascular endothelial cells lined up evenly,while some vascular endothelial cells broke the internal limiting membrane occasionally.In model control group,a lot of vascular endothelial cells broke the internal limiting membrane and new vessel lumen was formed.The result of low UA intervention group was basically similar to that of model control group.The result of high-dose UA intervention group was close to that of positive control group,though some vascular endothelial cells broke the internal limiting membrane,and internal limiting membrane structure was even,and new vessel lumen was not formed.The endothelial nuclei number of newly-generated internal limiting membrane vessel in model control group was obviously higher than that of blank control group,while the high-dose UA intervention group was obviously lower than that of model control group,and the high-dose UA intervention group was lower than that of the low-dose UA intervention group obviously,and all the differences were statistically significant(P ＜ 0.05).Retinal flatmounts showed that in the blank control group retinal vascular appeared with uniformly radiated distribution from optic disc in all directions,with bigger pipe diameter,proper branch and clear peripheral retinal vascular structure.However,lots of retinal neovascularization was seen in model control group,with slender retinal diameter,line rigidity,structure disorder,and uneven distribution,and there was large non-perfused areas in the centeral area.Through comparison with different doses UA intervention group,retinal vascular distribution and pattern in the high-dose UA intervention group were close to the positive group.The distribution of retinal neovascularization was much better than the model control group and the low-dose UA intervention group,with no obvious non-perfused areas.Conclusions UA can inhibit the formation of neovascularization in oxygen-induced mice retinal ischemia model.It has a positively correlated relationship with UA dose.

Coupled external fixator and skin flap transposition for treatment of exposed and nonunion bone / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To discuss the effect of coupled external fixator and skin flap transposition on exposed and nonunion bones.</p><p><b>METHODS</b>The data of 12 cases of infected nonunion and exposed bone following open fracture treated in our hospital during the period of March 1998 to June 2008 were analysed. There were 10 male patients, 2 female patients, whose age were between 19-52 years and averaged 28 years. There were 10 tibial fractures and 2 femoral fractures. The course of diseases lasted for 12-39 months with the mean period of 19 months. All the cases were treated by the coupled external fixator and skin flap transposition.</p><p><b>RESULTS</b>Primary healing were achieved in 10 cases and delayed healing in 2 cases in whom the tibia was exposed due to soft tissue defect and hence local flap transposition was performed. All the 12 cases had bony union within 6-12 months after operation with the average time of 8 months. They were followed up for 1-3 years and all fractures healed up with good function and no infection recurrence.</p><p><b>CONCLUSION</b>The coupled external fixator and skin flap transposition therapy have shown optimal effects on treating infected, exposed and nonunion bones.</p>

Clinical observation on scalp point injection to improve the cerebral microcirculation for children of early cerebral palsy / 中国针灸

<p><b>OBJECTIVE</b>To explore an effective therapy for children of early cerebral palsy.</p><p><b>METHODS</b>One hundred and twenty cases were randomly divided into an acupoint injection group and a medication group, 60 cases in each group. The acupoint injection group was treated with scalp point injection of 0.5-1 mL brain protein hydrolysate into each point, Baihui (GV 20), Fengchi (GB 20) and motor area etc. were selected; the medication group was treated with 10 mL brain protein hydrolysate by intravenous drip. The therapeutic effects in the two groups were observed, and the changes of cerebral blood flow were compared before and after treatment.</p><p><b>RESULTS</b>The total effective rate of 91.4% in the acupoint injection group was superior to that of 73.7% in the medication group (P<0.05), the acupoint injection group could significantly improve the systolic peak velocity (Vs), end diastolic velocity (Ved) and mean velocity (Vm) of middle cerebral artery (MCA) and anterior cerebral artery (ACA), and decrease the vascular resistance index (RI) (all P<0.05), and the improvement degree was superior to the medication group (all P<0.05).</p><p><b>CONCLUSION</b>Scalp point injection therapy can significantly improve the cerebral microcirculation of patients, and has a good therapeutic effect for early cerebral palsy.</p>

Dendritic cells loaded with U251 tumor cell antigen enhance the cytotoxic effect of cytokine-induced killer cells against U251 cell line / 中华神经医学杂志

Objective To study the cytotoxic effect of cytokine-induced killer cells (CIKs) cocultured with U251 tumor cell antigen-loaded mature dendritic cells (DCs) against U251 cell line. Methods The DCs and CIKs were derived from the cord blood mononuclear cells (CBMCs) of the same donor. The DCs were challenged with U251 tumor cell antigen, and cocultured with the CIKs to induce the cell complex Ag-DC-CIK. The mature DCs were identified by morphological and phenotypic analyses. MTT assay was performed to detect the cytotoxic effects ofCBMCs, CIKs, antigen-loaded CIKs (Ag-CIK) or the cell complex Ag-DC-CIK in U25 ! cells. Results The mature DCs derived from the CBMCs highly expressed the costimulatory molecules CD86 (82.66%) and CD40 (69.40%), and moderately expressed CD83 (57.49%) and CD80 (51.14%). The cytotoxic activity of the cell complex Ag-DC-CIK against U251 cells (58.8%) was significantly higher than those of CBMCs (29.71%), CIKs (39.89%), and Ag-CIK (49.92%). Statistical analysis indicated significant difference in the cytotoxic activity between any two of the groups (P<0.05). Conclusion The DCs loaded with the tumor cell antigen can enhance the cytotoxic effect of the CIKs against the target tumor cells, which sheds light on a new approach of immunotherapy for intracranial tumors.

Development of bovine blood lead component analysis standard reference materials / 中华预防医学杂志

<p><b>OBJECTIVE</b>To develop the first grade standard analytical reference material of bovine blood lead.</p><p><b>METHODS</b>According to standards and technology specification of primary standard reference material (JJG1006-1994), ISO 17511, and volume-effect relationship, a bovine blood lead model was developed by adding acetate lead in the feed in dose of 2-5 mg x kg(-1) x d(-1). Cow blood was collected when blood lead concentration went up to low, medium and high concentration range (90-100, 190-200, 280-300 microg/L). Blood sample was kept in tube and frozen after irradiation. The samples were tested for homogeneity and stability. ID-MS method was used to measure the lead concentration through comparison with two standard lead samples from the USA NIST SRM 955b.</p><p><b>RESULTS</b>Samples of the three lead concentrations showed uniformity by single factor analysis of variance (F = 0.61, 1.64, 0.28, respectively, P > 0.05) . After 14 months monitoring, the RSD was 0.85%, 1.05% and 0.49% (t = 0.787, 1.132, 0.854, respectively, P > 0.05). The characteristic and indefinite values were 102.4 +/- 5.5; 181.2 +/- 4.0; 304.5 +/- 3.9, respectively (unit: microg/L). The reproduction of the two USA NIST SRM 955b samples further showed our methods and procedures were correct. Our sample was stabile for four days at room temperature.</p><p><b>CONCLUSION</b>The standard reference material of bovine in our research had reached the national standard material requirements.</p>

An animal model of venous congested flap for long-term microcirculation study / 中华整形外科杂志

<p><b>OBJECTIVE</b>To set up a venous congested flap model to study the mechanism of necrosis through long-term microcirculation observation.</p><p><b>METHODS</b>A specially deviced chamber was assembled to one side of the ears in an adult white rabbit, about 7 approximately 10 days after the operation the congested flap model was made and the microcirculatory status of the flap was dynamically observed under a vivo-microscope for a long time.</p><p><b>RESULTS</b>The venous crisis phenomenon of flap was well studied and the microcirculation of the flap was observed carefully, finally the variational rule of the congestion flap microcirculation was made clear.</p><p><b>CONCLUSIONS</b>The model could well simulate the venous crisis flap in clinic, and the microcirculation could also be observed for a long time.</p>

Role of erythrocyte on brain edema development after traumatic intracerebral hemorrhage in rats / 中华创伤杂志

Objective To study the effects of erythrecyte on brain edema after traumatic intrace- rebral hemorrhage(TICH)and explore the mechanisms of erythrocyte in brain edema development follow- ing TICH.Methods Firstly,the brain injury model of SD rat was established by applying a free-fall- ing device,then whole blood(WB),lysed RBC(LRBC)or parked RBC(PRBC)were infused with ste- reotactic guidance into injured cortex to produce the model of TICH.All rats were killed at 1,3,and 5 days after injury.The brain water content was measured,immunohistochomistry(SABC)was applied to test HO-1 and TNF-?expressions.Results 1.In WB group,PRBC group and TBI group,the brain water content was the highest on the third day.The brain water content of LRBC group was markedly higher on the first day than on the third and fifth days.Comparison among the four groups showed the wa- ter content was the highest on the 1st day in LRBC group,and on the 3rd day in WB and PRBC groups; there was no significant difference among the four groups on 5th day.2.The positive expression of HO-1 and TNF-?coincided with the change of the water content in groups of WB,PRBC and LRBC.Conclu- sions In rat model of TICH,RBC plays an important role in delayed brain edema formation(3 days after injury),but has no influence at early stage(1 day after injury).The mechanisms of delayed brain edema involves RBC breakdown and inflammation reaction.