The Effects of Estrogen on Detrusor Contraction and the Expression of Muscarinic Receptors in Ovariectomized Rats / 대한비뇨기과학회지

PURPOSE: The purpose of this study was to evaluate the effects of estrogen on detrusor contraction and the expression of muscarinic receptors in ovariectomized rats. MATERIALS AND METHODS: 24 Sprague-Dawley female virgin rats(12 weeks old) were separated into three groups of 8 rats each. Group I served as a control group, group II was the ovariectomized only rats(Ovx group) and Group III was given estradiol benzoate(0.8mg/kg/day) subcutaneously for 7 consecutive days, beginning 1 week after ovariectomy(Ovx+E group). At the end of the experimental period, each rat was sacrificed and the urinary bladder was removed for contractile studies. The expressions of M2 and M3 receptors in the bladder epithelium and the muscle layer were investigated by performing immunofluorescent staining. RESULTS: The Ovx group showed a significantly decreased bladder contractile function on the KCl and carbachol-induced contractile tests, whereas the Ovx+E group showed increased contractility(p<0.05). The Ovx+E group showed an increase of smooth muscle compared to the other groups. Ovariectomy induced a significant increase in the M3 receptors density in the bladder body, as compared to the control group(p<0.05) but there was no significant difference between the Ovx group and the Ovx+E group. CONCLUSIONS: Bladder dysfunction of menopausal women is thought not to be related with the changes of muscarinic receptors. Our results suggest that the detrusor contractility of menopausal women might be improved after estrogen replacement therapy.

The Influences of Bladder Outlet Obstruction on Improvement of Storage Symptoms in Patients Who Underwent Transurethral Resection of Prostate / 대한비뇨기과학회지

PURPOSE: To investigate the influences of preoperative bladder outlet obstruction(BOO) to be adjusted by age and prostate volume on improvement of storage symptoms in patients who underwent transurethral resection of prostate(TURP) in a long term follow up. MATERIALS AND METHODS: We performed retrospective study of 75 patients with benign prostatic hyperplasia(BPH) who had undergone TURP and preoperative urodynamics from July 2004 to December 2006. 18 patients of them were lost follow up in this period, so we analyzed 57 patients in this study. The degrees of BOO were measured by the Abrams-Griffiths (AG) number calculated according to the preoperative urodynamic data. International Prostate Symptom Score(IPSS) questionnaire were used to evaluate improvement of symptoms. Especially IPSS questions number 2, 4 and 7 were used to evaluate storage symptoms. Partial correlation analysis was done between the AG numbers and the degree of storage symptoms improvement, adjusted by the age and the prostate volume. RESULTS: There were significant decreases of the IPSS(p=0.029), storage symptom score(p=0.024) and frequency score(p=0.017) in BOO group compared to the non-BOO group. The changes of IPSS(gamma=-0.338, p=0.012), storage symptoms score(gamma=-0.292, p=0.031) and frequency symptom score (gamma=-0.305, p=0.023) were significantly correlated with AG number. CONCLUSIONS: These results suggested that the preoperative AG number derived from urodynamics was an important factor in predicting the postoperative results. It would be expected that the patients with high AG numbers might be more improved storage symptom after TURP.