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Objective Scutellaria baicalensis stems and leaves glucuronic hydrolase(sbsl GUS)was used to enzymatically hydrolyze scutellarin in Erigeron breviscapus(Vant.)Hand.Mazz.to prepare scutellarein,and the high-purity scutellarein was obtained through separation and purification.Methods Orthogonal experiments were used to optimize the process parameters for the extraction of Erigeron breviscapus(Vant.)Hand.Mazz..Using the rate of enzymatic hydrolysis conversion of scutellarin as the index,the amount of enzyme,pH,temperature,time and antioxidant were investigated,and the preparation process parameters of scutellarein were optimized.Ethanol extraction,activated carbon decolorization,and fractional crystallization were used to purify the crude extract.Results The extraction process was determined to be:segments of Erigeron breviscapus were decocted twice with 10 times water for 1 hour each time.The preparation process of scutellarein was as follows:the amount of sbsl GUS extract and Erigeron breviscapus decoction was 1∶10 based on crude drugs,0.5%sodium metabisulfite was added,pH value was about 6.0,the temperature was about 45℃,and the time was 20 hours.The crude extract of scutellarein with the content more than 60%was obtained.The crude extract was purified by fractional crystallization,refluxed with 80%ethanol,decolorized with activated carbon,concentrated and crystallized,and the scutellarein extract with content more than 85%was obtained.Conclusion sbsl GUS enzymatic hydrolysis technology,which was used to prepare scutellarein,is simple and feasible.This study provides a new way for the manufacture of scutellarein.
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ObjectiveTo analyze the characteristics of kidney Yang deficiency syndrome in different stages and time evolution of chronic kidney disease (CKD) to explore the evolution patterns of kidney Yang deficiency syndrome in CKD. MethodThe evidence information of 256 patients with CKD was collected from October 2020 to September 2022 according to relevant standards, and the "Kidney Yang Deficiency Syndrome Evaluation Scale for Chronic Kidney Disease" was developed. With SPSS Statistics 20.0, SPSS Modeler 18.0, Gephi 0.9.2, and R 4.2.1, the syndrome information of CKD patients at various stages and the syndrome changes after one year were statistically analyzed using complex network analysis, association rule analysis, probability transition matrix analysis, and chi-square test, and the kidney Yang deficiency syndrome of patients at various stages was comprehensively evaluated. ResultIn the CKD population, the proportion of females with kidney Yang deficiency syndrome was higher than that of males (P<0.01), and the proportion of people over 65 years old was higher than in people under 65 years old. The proportion of people with kidney Yang deficiency syndrome increased with the progression of kidney disease, and the proportion of Ⅳ-Ⅴ CKD patients with kidney Yang deficiency syndrome was higher than that of Ⅰ-Ⅱ CKD patients (P<0.01). From Ⅰ CKD to Ⅴ CKD, the frequency of dull tongue continued to increase, and the frequency of enlarged tongue and tooth-marked tongue continued to increase after Ⅲ CKD. The frequency of thick coating and greasy coating ranked in the top 3 of frequency distribution in Ⅴ CKD. After Ⅲ CKD, the top 3 tongue characteristics were weak pulse, deep pulse, and thready pulse, all of which were characteristics of kidney Yang deficiency syndrome. Complex network analysis of the tongue and pulse showed that the core tongue and pulse characteristics of patients with end-stage CKD were tooth-marked tongue with white coating and deep and thready pulse. The results of symptom frequency analysis and complex network analysis showed that aversion to cold and preference for warmth, weakness of the knees, and cold extremities were the top 3 symptoms in Ⅰ-Ⅲ CKD patients with kidney Yang deficiency syndrome, and in Ⅳ-Ⅴ CKD, the manifestations of the syndrome of Yang deficiency and water diffusion, such as drowsiness and fatigue, edema, and frequent urination at night became characteristic symptoms. The scores of edema, pale complexion, soreness and weakness of the waist and knees, loose stools, and mental depression symptoms, as well as the total score of kidney Yang deficiency syndrome gradually increased with disease progression, with statistical differences between different stages of CKD (P<0.05, P<0.01). The frequency analysis of disease-related syndrome elements showed that the frequencies of Yang deficiency syndrome, phlegm-dampness syndrome, blood stasis syndrome, and turbidity-toxin syndrome gradually increased with disease progression, and there were statistically significant differences in the distribution between different stages of CKD (P<0.05, P<0.01). The results of complex network analysis showed that Yang deficiency syndrome was the core syndrome element throughout all stages of CKD and was the main syndrome element type of CKD, while phlegm-dampness syndrome, blood stasis syndrome, and turbidity-toxin syndrome were gradually revealed in the middle and late stages of CKD. In the CKD population with kidney-Yang deficiency syndrome, the distribution of phlegm-dampness syndrome, blood stasis syndrome, and turbidity-toxin syndrome as concurrent syndromes in different CKD stages had statistically significant differences (P<0.05, P<0.01). The association rule analysis showed that as the disease progressed, associations between the concurrent syndromes, such as phlegm-dampness syndrome, blood stasis syndrome, turbidity-toxin syndrome, and fluid retention syndrome, and kidney-Yang deficiency syndrome were gradually enhanced. The comparison of the changes in CKD with kidney Yang deficiency syndrome within one year showed that the disease location was centered on the kidney and transmitted between the spleen, stomach, heart, and liver. There is a 23.81% probability of kidney-Yang deficiency syndrome transforming into Qi deficiency syndromes (Qi deficiency in the spleen and kidney, Qi deficiency in the liver, and Qi deficiency in the heart), 23.79% into Yin deficiency syndromes (Yin deficiency in the liver and kidney, Qi and Yin deficiency, and Yin deficiency in the liver and stomach), and 9.52% into dampness syndromes (phlegm-dampness internal obstruction and wind-dampness obstruction). In contrast, 20% of spleen and kidney Qi deficiency syndrome transformed into kidney Yang deficiency syndrome, and 33.33% of Qi deficiency and blood stasis syndrome transformed into kidney Yang deficiency syndrome. ConclusionAs Ⅰ CKD progresses to Ⅴ CKD, the severity of kidney Yang deficiency syndrome gradually increases, and the syndrome characteristics of kidney Yang deficiency become pronounced. Furthermore, the pathogenic factors, such as phlegm-dampness, blood stasis, and turbidity-toxin, gradually increase. With the change of time, kidney Yang deficiency syndrome in CKD tends to evolve into syndromes related to Qi deficiency, Yin deficiency, and dampness. The discovery of these rules provides a theoretical basis and reference guidance for the treatment of CKD based on syndrome differentiation.
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Objective Through factor analysis of the quantified syndrome information of 558 cases of kidney yang deficiency syndrome,the constructing feature of kidney yang deficiency syndrome was revealed,which provides clinical data support for the objectification,standardization and normalization of kidney Yang deficiency syndrome.Methods Firstly,the frequency analysis of symptoms,tongue and pulse signs of 558 patients with kidney Yang deficiency syndrome was carried out,and then the main syndrome information of the patients with kidney Yang deficiency syndrome was quantified.Finally,the common factors and their representative variables of kidney Yang deficiency syndrome were screened out through factor analysis,and the constructing feature of kidney Yang deficiency syndrome was analyzed combined with TCM syndrome knowledge.Results Eight common factors with eigenvalues greater than 1 were extracted by principal component analysis,and the cumulative contribution rate was 60.483%.After the factor rotation,the representative variables with the absolute value of load coefficient greater than 0.45 in each common factor were selected.The representative variables of F1 are afraid of cold and fond of warmth(0.947)and intolerance to cold(0.932).The representative variables of F2 are waist pain(0.754),waist and knee weakness(0.720)and cold in waist and knees(0.466).The representative variables of F3 are depression(0.749),insomnia(0.711)and diarrhoea(0.470).The representative variables of F4 are thin fur(0.819)and white fur(0.768).The representative variable of F5 are tinnitus and deafness(0.687),frequent nocturnal urination(0.591)and decreased libido(0.587).The representative variables of F6 are pulse sinking(0.766)and pulse weakness(0.736).The representative variables of F7 is thready pulse(0.942).The representative variable of F8 is pale tongue(0.961).External syndrome of disease location involved in these common factors are waist,bone,brain,ear,anterior Yin,posterior Yin and reproductive function.The disease nature involved in these common factors is deficiency and cold.Conclusion The basic constituent units of kidney Yang deficiency syndrome include disease location syndrome elements and disease nature syndrome elements.The disease location is kidney,and the abnormal changes of kidney location are mainly external symptoms of waist,bone,brain,ear,anterior Yin,posterior Yin and reproductive function.Its disease nature is deficiency and cold.Yang deficiency leads to external cold.Yang Qi deficiency can not warm the body surface resulting in the appearance of external cold syndrome.
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OBJECTIVE@#To depict the cell landscape and molecular biological characteristics of human intrauterine adhesion (IUA) so as to better understand its immune microenvironment and provide new inspirations for clinical treatment.@*METHODS@#Four patients with IUA who underwent hysteroscopic treatment at Dongguan Maternal and Child Health Care Hospital from February 2022 to April 2022 were selected as the study subjects. Hysteroscopy was used to collect the tissues of IUA, which were graded based on the patient's medical history, menstrual history and status of IUA. Library construction, sequencing, single cell data comparison and gene expression matrix construction were carried out in strict accordance with the single cell RNA sequencing process. Thereafter, the UMAP dimension reduction analysis of cell population and genetic analysis were carried out based on the cell types.@*RESULTS@#A total of 27 511 cell transcripts were obtained from four moderately graded IUA tissue samples and assigned to six cell lineages including T cells, mononuclear phagocytes, epithelial cells, fibroblasts, endothelial cells and erythrocytes. Compared with normal uterine tissue cells, the four samples showed different cell distribution, and the proportions of mononuclear phagocytes and T cells in sample IUA0202204 were significantly increased, suggesting a strong cellular immune response.@*CONCLUSION@#The cell diversity and heterogeneity of moderate IUA tissues have been described. Each cell subgroup has unique molecular characteristics, which may provide new clues for further study of the pathogenesis of IUA and heterogeneity among the patients.
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Grossesse , Femelle , Enfant , Humains , Cellules endothéliales , Maladies de l'utérus/complications , Hystéroscopie/méthodes , Adhérences tissulaires/étiologie , Analyse de séquence d'ARNRésumé
ObjectiveTo study the correlations of the characteristics of kidney Yang deficiency syndrome in patients with chronic kidney disease (CKD) with clinical indicators and to explore the risk factors of kidney Yang deficiency in CKD. MethodThe differentiation of traditional Chinese medicine (TCM) syndrome classified the 225 CKD patients who met the inclusion criteria into two groups: one group of kidney Yang deficiency syndrome (99 patients) and one group of non-kidney Yang deficiency syndrome (126 patients). The symptoms, tongue manifestation, pulse manifestation, and accompanied symptoms of the kidney Yang deficiency syndrome group were recorded. The syndrome characteristics were summarized by factor analysis and clustering analysis. The levels of hemoglobin, red blood cell count, urinary protein, urinary glucose, creatinine, urea nitrogen and glomerular filtration rate were compared between the kidney Yang deficiency syndrome group, the non-kidney Yang deficiency syndrome group and the normal control group by ANOVA and non-parametric test. The binary logistic regression model was employed to analyze the correlations of lifestyle, body mass index (BMI) with syndrome. ResultThe high-frequency symptoms of CKD patients with kidney Yang deficiency syndrome were waist pain, fear of cold, favor of warm, lethargy, fear of cold at waist and knees, etc. The patients mainly presented deep pulse, thready pulse, or weak pulse, and the tongue with white coating, greasy coating, or thin coating. A total of 13 common factors were obtained, which can be classified into 5 categories. The patients with kidney Yang deficiency syndrome mainly had symptoms in limbs (especially lower limbs), chest, bladder, fleshy exterior, and stomach, with the main manifestations of deficiency-cold, Qi deficiency, fluid retention, and blood stasis. The clustering analysis classified the patients into 11 categories, which reflected that kidney Yang deficiency syndrome mainly presented the symptoms of Qi deficiency, blood stasis, and fluid retention, with fleshy exterior, limbs, spleen, stomach, ears, mind, and bladder involved. The results of clustering analysis and factor analysis were consistent, both of which indicated that the patients were weak with deficiency-cold, accompanied by fluid retention and blood stasis. Frequency analysis also showed that common symptoms mainly included Qi deficiency, fluid retention, cold-dampness, and blood stasis. Compared with the non-kidney Yang deficiency group, the kidney Yang deficiency group showed a large proportion of patients in stage 3-5 CKD, elevated urea nitrogen (P<0.05), decreased glomerular filtration rate, hemoglobin, and red blood cell count (P<0.05), and increased qualitative grade of urine protein. In addition, the results of regression analysis showed that female, little or no exercise, and diet preference were the risk factors for kidney Yang deficiency syndrome in CKD (P<0.05). ConclusionThe disease location and manifestations have correspondence in the CKD patients with kidney Yang deficiency syndrome. The TCM symptoms are correlated with clinical indicators. Hemoglobin, red blood cell count, glomerular filtration rate, urea nitrogen, and urine protein can reflect the connotation of kidney Yang deficiency syndrome in CKD to a certain extent. Additionally, related risk factors in life can affect the occurrence of kidney Yang deficiency syndrome in CKD.
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ObjectiveTo study the correlations of the characteristics of kidney Yang deficiency syndrome in patients with chronic kidney disease (CKD) with clinical indicators and to explore the risk factors of kidney Yang deficiency in CKD. MethodThe differentiation of traditional Chinese medicine (TCM) syndrome classified the 225 CKD patients who met the inclusion criteria into two groups: one group of kidney Yang deficiency syndrome (99 patients) and one group of non-kidney Yang deficiency syndrome (126 patients). The symptoms, tongue manifestation, pulse manifestation, and accompanied symptoms of the kidney Yang deficiency syndrome group were recorded. The syndrome characteristics were summarized by factor analysis and clustering analysis. The levels of hemoglobin, red blood cell count, urinary protein, urinary glucose, creatinine, urea nitrogen and glomerular filtration rate were compared between the kidney Yang deficiency syndrome group, the non-kidney Yang deficiency syndrome group and the normal control group by ANOVA and non-parametric test. The binary logistic regression model was employed to analyze the correlations of lifestyle, body mass index (BMI) with syndrome. ResultThe high-frequency symptoms of CKD patients with kidney Yang deficiency syndrome were waist pain, fear of cold, favor of warm, lethargy, fear of cold at waist and knees, etc. The patients mainly presented deep pulse, thready pulse, or weak pulse, and the tongue with white coating, greasy coating, or thin coating. A total of 13 common factors were obtained, which can be classified into 5 categories. The patients with kidney Yang deficiency syndrome mainly had symptoms in limbs (especially lower limbs), chest, bladder, fleshy exterior, and stomach, with the main manifestations of deficiency-cold, Qi deficiency, fluid retention, and blood stasis. The clustering analysis classified the patients into 11 categories, which reflected that kidney Yang deficiency syndrome mainly presented the symptoms of Qi deficiency, blood stasis, and fluid retention, with fleshy exterior, limbs, spleen, stomach, ears, mind, and bladder involved. The results of clustering analysis and factor analysis were consistent, both of which indicated that the patients were weak with deficiency-cold, accompanied by fluid retention and blood stasis. Frequency analysis also showed that common symptoms mainly included Qi deficiency, fluid retention, cold-dampness, and blood stasis. Compared with the non-kidney Yang deficiency group, the kidney Yang deficiency group showed a large proportion of patients in stage 3-5 CKD, elevated urea nitrogen (P<0.05), decreased glomerular filtration rate, hemoglobin, and red blood cell count (P<0.05), and increased qualitative grade of urine protein. In addition, the results of regression analysis showed that female, little or no exercise, and diet preference were the risk factors for kidney Yang deficiency syndrome in CKD (P<0.05). ConclusionThe disease location and manifestations have correspondence in the CKD patients with kidney Yang deficiency syndrome. The TCM symptoms are correlated with clinical indicators. Hemoglobin, red blood cell count, glomerular filtration rate, urea nitrogen, and urine protein can reflect the connotation of kidney Yang deficiency syndrome in CKD to a certain extent. Additionally, related risk factors in life can affect the occurrence of kidney Yang deficiency syndrome in CKD.
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Objective:To examine the infection of the enterovirus and human herpes virus in children with suspected encephalitis.Methods:A total number of 365 suspected encephalitis cases were included in this study from August 2017 to December 2019 in Hunan Children′s Hospital. The clinical samples, the cerebrospinal fluid (CSF), serum, sputum, stool and urine were collected and preserved at-80 ℃condition. The enterovirus (EV) and human herpesvirus (HHV) were examined by a one-step nested reverse transcription PCR(RT-PCR) and a real-time fluorescent quantitative PCR (qPCR), respectively. The positive rate of the two viruses in clinical specimens of children with suspected encephalitis was examined. Among all cases, 132 cases were diagnosed with EV encephalitis or HHV encephalitis.Results:the EV encephalitis were identified in 20.5% (75/365) children with suspected viral encephalitis; whereas HHV encephalitis infection was identified as 15.6% (57/365). Among the 75 cases of EV encephalitis, echo 6 was the main sub-type of these diseases 52.0% (39/75) and others were EV71 (30.7%, 23/75), echo11 (6.7%, 5/75), Coxsackie virus A group 6(CA6, 4.0%, 3/75), echo30 (1.3%, 1/75), echo9 (1.3%, 1/75), echo4 (1.3%, 1/75),Coxsackie virus B group 1(CB1, 1.3%, 1/75))and poliovirus(1.3%, 1/75).Human herpes virus type 6 (HHV6) was the most common pathogen in 57 cases of HHV encephalitis, accounting for 35.1% (20/57).The other pathogens were Cytomegalovirus (CMV, 31.6%, 18/57), Epstein-Barr virus (8.8%, 7/57), Herpes simplex virus 1 (HSV1, 10.5%, 6/57), HSV2 (8.8%, 5/57), and Varicella zoster virus (VZV, 1.8%, 1/57) .The virus in CSF detected significantly earlier than that in serum after onset. Virus could be detected in CSF 2-7 days after onset,but 7-26 days in serum. Conclusions:This study uses nested PCR and qPCR to detect pathogens in clinical specimens of children. This not only expands our understanding of the clinical examination and diagnosis of viral encephalitis in children, but also promotes the method of this study to benefit more children.
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Objective:To compare anti-mullerian hormone (AMH) , sex hormone and inhibitor B (Inhibin B, INH-B) levels in children with different karyotypes, ages, and gender disorders of sex developmemt (DSD).Methods:A total of 101 patients with suspected gonadal dysplasia in children who underwent serological examination at the Children′s Hospital of Hunan Province from January 2019 to June 2019 were finally diagnosed by pathological biopsy. With reference to previous studies of the same type, the 101 patients included in this study were divided into 4 levels (<1 year old, 1-2 years old, 2-4 years old, >4 years old), and the social gender was divided into two levels: male and female. At the same time, 89 cases of normal gonadal development children without endocrine abnormality were selected as control. Serum levels of AMH, INH-B, luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), prolactin (PRL) and testosterone (T) were measured by chemiluminescence method.Results:Among the 101 cases, 62 were male and 39 were female; aged 23 days to 12 years, with a median age of 3.3 years; karyotype: 52 cases were 46, XX; 21 cases were 46, XY; 12 cases were 45, X; 7 cases were 46X, del (Xq); 5 cases were 46X, i (Xq); 2 cases were 45X, inv9; 2 cases were 45X / 46XX. There were 65 cases of partial gonadal dysplasia, 25 cases of disappearing testicular syndrome, and 11 cases of mixed gonadal dysplasia. One patient had a family history of infertility. Among the causes of children′s consultation, the most common were abnormal appearance of the external genitalia (54 cases, 53.47%), followed by small penile development and / or scrotal emptiness (25 cases, 24.75%). Other reasons included primary amenorrhea, double lateral groin mass, hypertension, clitoral hypertrophy, and labia minora adhesions. The levels of serum AMH, INH-B, and T in the gonadal dysplasia group were significantly higher than those in the normal gonadal development group, while the levels of LH, FSH, E2, and PRL were significantly lower than those in the normal gonadal development group ( P<0.05). The INH-B level of children with gonadal dysplasia in different age groups was statistically significant ( P<0.05), in which the INH-B level was the highest in <1-year-old children with gonadal dysplasia, and the lowest in 2-4-year-old children with gonadal dysplasia; the LH, FSH, E2, PRL, T levels of 46, XX and other karyotypes were statistically significant ( P<0.05); Compared with other age groups, the levels of LH, FSH, E2, and PRL were relatively higher in >4 year-old children with gonadal dysplasia, while the level of T was relatively lower; There were significant differences in E2, PRL and T levels in children with gonadal dysplasia in different age groups of 46, XY karyotype ( P<0.05). Compared with other age groups, E2, PRL and T levels of children with gonadal dysplasia >4 year-old old were relatively higher and T levels were relatively lower. The levels of AMH, LH, FSH, E2 and PRL in boys with glandular dysplasia were lower than those in girls ( P<0.05), while the levels of INH-B and T were higher in boys than those in girls ( P<0.05). Conclusions:The levels of anti-mullerian hormones, inhibin B, and sex hormones in children with gonadal dysplasia are different from the normal population, and may be related to the age, chromosome karyotype, and gender distribution of the child, but there are some confounding factors (such as etiology, treatment Scheme), so more samples are needed to verify it.
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Objective To understand the level and correlation of biochemical joint indicators in stroke high risk population in Yi-chun City ,and the differences in biochemical joint indicators between community and rural stroke high risk populations .Methods Three hundreds and fifty-four high risk people during stroke screening in 2015 and 2016 included 115 community high risk cases , 239 rural cases ,226 males and 128 females ,aged 40 to 86 years old .Thirty-one cases of non-stroke high risk included 15 cases of male and 16 cases of female ,aged 28 to 85 years old .Seven biochemical joint indicators were detected and statistically analyzed ,in-cluding glucose (GLU) ,glycated hemoglobin(HbA1c) ,total cholesterol(TC) ,triacylglycerin (TG) ,high-density lipoprotein choles-terol(HDL-C) ,low-density lipoprotein cholesterol (LDL-C) and homocysteine (Hcy) .Results In the comparison between stroke high risk population and non-high risk populations ,CH ,TG ,LDL-C and Hcy had statistically significant difference (P0 .05) .In the comparison between community and rural stroke high risk populations ,CH ,TG ,LDL-C and GLU had statistically significant difference (P0 .05) .Conclusion The increase of CH ,TG ,LDL-C and Hcy is the risk factors of stroke , while CH ,TG ,LDL-C and Hcy are the interventional risk factors and play an important role in its early diagnosis ,treatment and prognosis .The key to prevent stroke is the healthy diet ,good living habits ,moderate exercises ,and regular monitoring of CH ,TG , LDL-C ,Hcy and GLU ,forewarning ,prevention and treatment ,which can avoid the occurrence or less occurrence of stroke ,thus re-duces the its incidence rate ,greatly decrease the morbidity ,recurrence rate ,and reduces the familial and social burden of manpower , material resources and financial resources .
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Objective To analyze the relationship between intrahepatic expression of HBsAg and HBcAg and the serum HBsAg and the load of HBV DNA in low-level HBsAg.To provide the basis for the diagnosis of low-level HBsAg.Methods A total of 63 patients with HBsAg lower than 1 400 IU/mL and higher than 0.05 IU/mL were enrolled in the study.All the 63 patients with low level HBsAg were collected for liver biopsy from January 2013 to December 2015.Serum HBsAg was detected by Roche cobas e601 automatic electrochemiluminescence immunoas-say system and supporting reagents.HBV DNA was detected using ROCHE automatic AmpliPrep/cobas TaqMan 48 system detector and supporting reagents.Results Among the 63 patients,the HBsAg in liver tissue was negative in 5 cases(7.94%),+in 47 cases(74.60%),++in 8 cases(12.70%),+++in 2 cases(3.17%),and++++was found in 1 case.The expression of HBcAg were negative in 30 cases(47.62%),+in 28 cases(44.44%),++in 4 cases(6.35%),+++in 1 case(1.59%),and++++in 0 cases.There was a significant positive correlation between HBsAg expression and serum HBsAg level by spearman and kendall correlation analysis(spearman analysis:r = 0.261,P=0.039;kendall analysis:r=0.217,P=0.036).HBcAg expression was significantly correlated with serum HBV DNA load with spearman and kendall correlation analysis(spearman analysis:r=0.305,P=0.015;kendall analysis:r=0.259,P=0.017).Conclusions The expression of HBsAg in liver tissue of in the patients with low level of HBsAg is positively correlated with the level of serum HBsAg,but not with the load of HBV DNA. There is significant positive correlation between HBcAg expression and serum HBV DNA load.
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Objective To explore the incidence and characteristics of thyroid disease in early pregnancy and to provide a scientific basis for eugenics .Methods The chemiluminescence immunoassay was used to detect the concentrations of serum thyrotropin (TSH) ,free thyroxine (FT4) ,triiodothyronine (FT3) and thyroid peroxidase antibody (TPOAb) in 776 early pregnant women (<12+6 weeks ,experiment group) and 100 non‐pregnant child‐bearing women (control group) .The test results were analyzed accord‐ing to the different diagnostic criteria of early pregnancy and non‐pregnant childbearing women .Results The serum concentrations of TSH ,FT4 and TPO‐Ab had statistical differences between the experimental group and the control group (P<0 .05) .The inci‐dence rate of thyroid diseases in early pregnant women was 35 .05% ,which was significantly higher than 15 .00% in non‐pregnant childbearing women ,and the difference was statistically significant (P<0 .05) .The thyroid diseases in pregnant women of the ex‐perimental group were mainly hypothyroidism (9 .28% ) and sub‐clinical hypothyroidism (22 .94% ) ,compared with the control group ,the difference was statistically significant(P<0 .05) .Conclusion Early pregnancy has high incidence rate of thyroid disease , which can lead to birth defects .Therefore ,screening early gestation thyroid diseases should be paid attention to provide theoretical basis for eugenics .
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Objective To explore the clinical value of combined detection of procalcitonin (PCT ) ,C reaction protein (CRP) and white blood cells (WBC) count in diagnosis of neonatal infectious pneumonia .Methods A total of 137 cases of neonatal infectious pneumonia ,including bacterial infection group of 72 cases and non‐bacterial infection group of 65 cases ,were selected .Another 63 healthy neonatus were enrolled as control group .Levels of PCT ,CRP and WBC were measured .Results Levels and positive rates of PCT ,CRP and WBC in bacterial infection group were significantly higher than the other two groups (P<0 .05) .The sensitivity and specificity of PCT were higher than CRP and WBC .The sensitivity of combined detection were further increased .Conclusion Diagnostic sensitivity and specificity of PCT could be higher than CRP and WBC .Combined detection of PCT ,CRP and WBC might be with important clinical value for the differential diagnosis of neonatal infectious pneumonia and observation of curative effect .
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<p><b>OBJECTIVE</b>To prospectively observe the long-term antiviral efficacy and safety of telbivudine (LDT) administered as a monotherapy and as a combination therapy with adefovir dipivoxil (ADV) in patients diagnosed with chronic hepatitis B (CHB) and positivity for hepatitis B e antigen (HBeAg).</p><p><b>METHODS</b>A total of 140 patients with HBeAg-positive CHB were randomly divided into treatment groups for LDT monotherapy (n = 75; 600 mg orally, once daily) and LDT+ADV combination therapy (n = 65; LDT 600 mg plus ADV 10 mg orally, once daily). The shortest treatment course was 96 weeks and the longest was 240 weeks. At treatment weeks 12, 24, 48?, 96, 144, 192, and 240 patients were tested for hepatitis B virus (HBV) DNA, HBeAg seroconversion and ALT normalization time; in addition, the incidence and type of adverse drug reactions were recorded. Data were statistically analyzed to determine the significance of differences observed between groups.</p><p><b>RESULTS</b>The rate of patients experiencing more than or equal to 2 log HBV DNA reduction was higher in the LDT + ADV group (92.3%(60/65) vs. LDT: 86.7%(65/75), X2 = 1.58). The HBV DNA negative rates of the LDT and LDT + ADV groups were 62.7% and 61.5% (X2 = 0.01) at week 24, 76.0% and 81.5% (X2 = 0.63) at week 48, 80.0% and 89.2% (X2 = 2.2) at week 96, 78.3% and 93.3% (X2 = 3.24) at week 144, 83.7% and 91.7% (X2 = 0.47) at week 192, and 93.3% and 88.9% at week 240 (comparison between two groups for each point P more than 0.05); both groups showed higher early and rapid sustained HBV DNA negative rates. For the HBeAg seroconversion, the rates of the LDT and LDT + ADV groups were 17.3% and 23.1% (X2 = 0.71) at week 24, 29.3% and 30.8% (X2 = 0.03) at week 48, 42.7% and 40.0% (X2 = 0.10) at week 96, 55.0% and 43.3% (X2 = 1.08) at week 144, 55.8% and 66.7% (X2 = 0.45) at week 192, and 63.3% and 66.7% at week 240; however, pairwise comparison showed no statistically significant differences between the groups (P more than 0.05). Similarly, there was no significant difference between the two groups in incidence of resistance at week 48 (4.0% and 1.5%), week 96 (5.3% and 3.1%), week 144 (10.0% and 3.3%, X2 = 1.23), week 192 (11.6% and 8.3%), and week 240 (13.3% and 11.1%) (all P more than 0.05). Three patients experienced muscle soreness (LDT, n = 2; LDT + ADV, n = 1) and two patients experienced increased creatine phosphokinase (LDT, n = 1; LDT + ADV, n = 1); all side effects resolved spontaneously or with symptom-appropriate treatment.</p><p><b>CONCLUSION</b>The long-term efficacy of LDT as a monotherapy or as a combination therapy with ADV was similar and the two different treatment approaches were associated with similar rates of resistance. The long-term safety was good for both treatment approaches.</p>
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Adolescent , Adulte , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Adénine , Utilisations thérapeutiques , Antiviraux , Utilisations thérapeutiques , Association de médicaments , Antigènes e du virus de l'hépatite virale B , Sang , Hépatite B chronique , Sang , Traitement médicamenteux , Phosphonates , Utilisations thérapeutiques , Études rétrospectives , Thymidine , Utilisations thérapeutiquesRésumé
<p><b>OBJECTIVE</b>To determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.</p><p><b>METHOD</b>By HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.</p><p><b>RESULT</b>Carboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).</p><p><b>CONCLUSION</b>This method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.</p>
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Asteraceae , Chimie , Atractyloside , Chromatographie en phase liquide à haute performance , Méthodes , Médicaments issus de plantes chinoises , Fruit , ChimieRésumé
A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
Sujets)
Chromatographie en phase liquide à haute performance , Méthodes , Evodia , Chimie , Alcaloïdes indoliques , Limonines , Extraits de plantes , QuinazolinesRésumé
<p><b>OBJECTIVE</b>To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.</p><p><b>METHOD</b>HPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.</p><p><b>RESULT</b>Baifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.</p><p><b>CONCLUSION</b>This method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.</p>
Sujets)
Aconitum , Chimie , Alcaloïdes , Chromatographie en phase liquide à haute performance , Méthodes , Médicaments issus de plantes chinoises , Normes de référence , Médecine traditionnelle chinoise , Normes de référence , Contrôle de qualitéRésumé
AIM:To prepare Wurenchun Dispersible Tablet(Fructus schisandrae Chinensis)and investigate the dissolution of it.METHODS:The optimal formulation of Wurenchun Dispersible Tablets was established by the single factor experiments.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was determined by HPLC.RESULTS:Wurenchun Dispersible Tablets prepared by the mixture of 50 g extraction and 125 g CaHPO_4 plus excipent(MCC∶CMS-Na 3∶1)could disintegrate and disperse well within 3 min in(20?1)℃ water.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was higher than 80% in 15 min.CONCLUSION:Wurenchun Dispersible Tablets were stable,dissolved fast and completely.The preparation could promote the dissolution of total lignans.