Correlation of muscle-derived microparticles and lactadherin proteins with hypercoagulability in patients with perihip fractures / 中华骨科杂志

Objective:To investigate the changes of skeletal muscle-derived microparticles (SKMPs) and their scaving proteins lactadherin in peripheral blood of patients with perihip fractures and their effects on blood coagulation. It provides a theoretical basis for clinical evaluation of hypercoagulability in patients with perioperative hip fractures.Methods:A total of 44 patients (72.87±13.9 years old) with perihip fractures from October 2020 to January 2021 were included in the fracture group, and 40 healthy people were included in the group without fracture. Activated clotting time (ACT) in fracture group and control group was measured by coagulation and platelet function analyzer. The level of SKMPs in peripheral venous blood was determined by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of MPs scaving proteins lactadherin in peripheral blood of fracture group and control group.Results:A total of 44 patients with perihip fractures were included. There were 33 (75%) femoral neck fractures and 11 (25%) intertrochanteric fractures. SKMPs concentration in peripheral blood of perihip fracture group 175.73/μl; postoperative fracture group 124.28/μl; Normal control group 90.69/μl. ACT was (377.5±76.2) s in the perihip fracture group, (498.1±97.1) s in the post-fracture group, and (505.7±44.0) s in the control group. Correlation analysis showed that there was a linear relationship between SKMPs count and ACT value in fracture patients ( r=-0.52, P<0.001). There was no significant correlation between SKMPs concentration and ACT value in peripheral venous blood of the post-fracture group ( P>0.05); Lactadherin protein concentration in perihip fracture group: 2 779.61 pg/ml; Fracture postoperative group 1 985.38 pg/ml; Normal control group 1 651.19 pg/ml. There is a positive correlation between Lactadherin concentration and SKMPs concentration with a correlation coefficient of 0.70. Conclusion:SKMPs plays a role in the hypercoagulability of patients with perihip fractures. Decompensation of lactadherin protein may be one of the causes of perioperative hypercoagulation in patients with hip fractures.

Gallbladder metastasis from renal clear cell carcinoma: a case report / 中华泌尿外科杂志

Renal clear cell carcinoma metastasis to the gallbladder is rare. This paper reported a case of gallbladder metastasis of renal clear cell carcinoma detected after two years of left radical nephrectomy. The patient underwent laparoscopic cholecystectomy and followed by postoperative targeted therapy of sunitinib. No new metastasis was found after 5 months of follow-up.

IgG4-related kidney disease presenting as a cystic renal mass: a case report and literature review / 中华泌尿外科杂志

Objective:To investigate the clinical characteristics, differential diagnosis and treatment of IgG4-related kidney disease (IgG4-RKD) presenting as a cystic renal mass.Methods:A 42-year-old male patient was found having a tumor in the left kidney in an annual physical examination. Subsequent CTU scan revealed a round shadow in the lower pole of the left kidney, with slightly lower, uneven density and obscure boundaries, which was slightly enhanced on contrast-enhanced CT imaging, suggesting a cystic mass (Bosniak category Ⅲ). Contrast-enhanced MRI showed a long T1, long T2 signal lesion in the lower pole of the left kidney, which was not obviously enhanced in the cortical phase but exhibited continuous and separated enhancement in parenchymal and delayed phases, suggesting a cystic renal cell carcinoma. A preoperative diagnosis of a left renal mass was made, for which the patient underwent a laparoscopic partial nephrectomy.Results:The resected cystic mass was grey white with obscured boundaries. Microscopically, the mass showed lymphoplasmacytic hyperplasia with lymphoid follicles, cystic formation, fibrosis hyperplasia and vascular obstruction. Immunohistochemistry revealed that the number of IgG4 (+ ) plasma cells was more than 50/HPF, and over 40% IgG (+ ) plasma cells were IgG4 (+ ). By virtue of the radiologic and pathologic findings, the patient was diagnosed with IgG4-RKD. At 2 weeks after operation, serum IgG level was 0.71g/L (reference: 0.03-2.01 g/L). Chest and abdominal CT scans performed 3 months after operation showed no evidence of other organ involvement and serum IgG4 level was within the normal range. Therefore no adjuvant therapy was prescribed.Conclusions:It is usually difficult to differentiate IgG4-RKD presenting as a cystic renal mass by radiography alone. The presence of other organ involvement, serum IgG4 test and renal biopsy can help the diagnosis and so as to avoid unnecessary surgery.

Analysis of therapeutic effect of laparoscopic tumor resection combined with aspiration in the treatment of central renal angiomyolipoma / 中华泌尿外科杂志

Objective To explore the clinical efficacy of laparoscopic tumor resection combined with aspiration for central renal angiomyolipoma(RAML).Methods Retrospective analysis of clinical data of 11 patients with central RAML between November 2015 and August 2018,including 8 females and 3 males,aged 35-58 years,mean (45.3 ± 4.8) years;4 cases on the left side,7 cases on the right side,including 4 endophytic tumors,7 exophytic tumors;body mass index (BMI) 22-27 kg/m2,mean (24.9 ±1.8) kg/m2.The maximum diameter of these tumors were 4.2-8.9 cm,with an average of (6.7 ± 2.4)cm.The preoperative serum creatinine was 53-85 μmol/L,with an average of (64.6 ± 16.4) μmol/L,and the glomerular filtration rate (GFR) of the affected side was 42-55 ml/min,with an average of (45.5 ±3.6) ml/min.Lateral kidney function is normal.8 patients underwent retroperitoneal laparoscopic surgery and 3 cases underwent laparoscopic surgery.Based on the preoperative imaging,tumor tissue was aspirated as much as possible by suction device if the tumor got maximal fat component and had not abundant blood supply.Clamp the renal artery in case of bleeding affected the surgery vision.On the contrary,if the tumor had abundant blood supply,the renal artery was clamped first,then removed the section of tumor with scissor and the remaining basal tumor was aspirated totally by suction device.The operation time,warm ischemia time,intraoperative blood loss,postoperative hospital stay,postoperative complications and surgical results were analyzed.Results All the 11 cases were successfully completed without conversion to open surgery,only one case got injury of renal collection system.The operation time was 95-126 min,mean (110.5 ± 12.8) min,and the blood loss was 120-200 ml,mean (155.6 ± 38.4) ml.The warm ischemia time was 22-31 min,mean (27.6 ± 3.3) min,and the postoperative hospital stay was 5-7 days.RAMLs were diagnosed by the postoperative pathological examination.The renal function was normal and no recurrence occurred in these patients at a mean follow-up of 13.9 months.All the 11 cases were successfully completed.Postoperative pathological diagnosis was RAML.There was no complication in the 11 cases.Preoperative serum creatinine and serum creatinine in the first month after surgery [(64.6 ± 16.4)μmol/L vs.(66.7 ± 15.5)tμmol/L,P =0.38] and preoperative renal GFR and GFR in the first month after surgery [(45.5 ± 3.6)ml/min vs.(43.2 ± 3.3) ml/min,P =0.067],the difference was not statistically significant.No recurrence was observed in the 11 cases with an average follow-up of 13.9 months.Conclusions Our initial experience suggests that laparoscopic tumor resection combined with aspiration is a safe,effective and minimally invasive method for the treatment of central RAML.

Conduction of a prostate cancer whole-cell vaccine modified by mice HSP70 and GM-CSF / 中国现代医生

Objective To construct a whole-cell vaccine modified by mice HSP70 and GM-CSF in prostate cancer. Methods Sequence fragments carrying mice Hspa1a coupled with C-myc or Csf2 coupled with V5 tag were constructed by chemical synthesis and PCR amplification , then both fragments were cloned into the vector pMD-18T. After se quencing, fragments described above were cloned into pLenti6.3_MCS lentiviral vector. The lentiviral expression vectors were named as pLenti6.3_MCS_Hspa1a_cMyc and pLenti6.3_MCS_Csf2_V5. These vectors were transfected or co-transfected into mice prostate cancer cell line RM-1 and proteins were detected by Western blotting. Following irradia-tion of 10 Gy, cells lost proliferative ability and were frozen and then stored. Results Recombinant lentiviral vectors pLenti6.3_MCS_Hspa1a_cMyc and pLenti6.3_MCS_Csf2_V5 were successfully constructed and GM-CSF, HSP70 or both expression in the transfected RM-1 cells were verified by Western blotting. Conlusion The RM-1 whole-cell vaccines modified by HSP70 and GM-CSF have been successfully constructed, which facilitate further investigation of the action mechanisms of whole-cell antigen and immune enhancement.

The expression and clinical significance of CD47 and calreticulin in infiltrating ductal breast carcinomas / 中国基层医药

Objective To evaluate the expression of CD47 and calreticulin and their relationships with clini-copathologic features,prognosis in infiltrating ductal breast carcinomas.Methods The expression of CD47 and calret-iculin was evaluated in 128 cases with infiltrating ductal breast carcinomas by means of immunohistochemistry,and the relationship between CD47 and other clinicopathologic factors as well as prognosis were analyzed.Results The expression level of CD47 in cancer nest was 81/128,which was lower than 106/128 in its adjacent normal epithelium (χ2 =12.400,P<0.05),but calreticulin expression was 50/128 and was higher in cancer nest(χ2 =21.510,P<0.05 ).In addition,the expression level of calreticulin had certain difference in different molecular subtype (χ2 =21.510,P<0.05),and an increased expression in Basal-like and Her-(2 +)/ER(-)subtypes.The expression level of CD47 was associated with tumor sizes,histological tumor grade and lymph node involvement(χ2 =11.400, 4.732,5.432,all P<0.05),and the expression level of calreticulin was associated with histological tumor grade and distant metastasis(χ2 =10.810,6.770,all P<0.05).The 5 -year survival rate of patients performed following-up was 68.37%.Univariate analysis indicated the survival rate in 5 years of high level CD47,calreticulin expression was higher than that of low level CD47,calreticulin expression(χ2 =5.231,13.069,all P<0.05).Conclusion The expression of CD47,calreticulin is associated with index of dysprognosis and survival rate in 5 years,so combined CD47 and calreticulin detect may be of prognostic value in patients with breast caner.

Influence on bladder spasm and life quality of comprehensive nursing in-tervention on transurethral resection of the prostate patients postoperative / 中国现代医生

Objective To explore the influence on bladder spasm and life quality of comprehensive nursing intervention on transurethral resection of the prostate patients postoperative. Methods Selected 80 cases from January 2010 to Jan-uary 2014 in our hospital underwent transurethral resection of the prostate, according to the different nursing methods the patients were randomly divided into intervention group and routine group, 40 cases in each group, all the patients underwent TURP operation, while the control group received routine nursing in perioperation period, intervention group focused on the comprehensive nursing implementation before and after the operation, bladder spasm, intervention on life quality after operation, QOL scoring, SAS, SDS scores preoperative and postoperative were compared between two groups. Results The bladder spasm rate of intervention group patients reached 10.0%, was significantly lower than that of the control group (30.0%), the difference was statistically significant (χ2=4.673, P<0.05). After the intervention, two groups of patients with QOL were significantly higher than those before intervention, and the intervention group was significantly higher than that of the control group, the difference was statistically significant(P<0.05). After the interven-tion, two groups of patients with SAS score, SDS score were significantly lower than those before intervention, and the intervention group decreased more significantly than the control group, the difference was statistically significant (P<0.05). Conclusion Transurethral resection of the prostate in patients with the implementation of comprehensive nursing intervention measures, can reduce the incidence of postoperative bladder spasm, improve the BPH life quality of pa-tients, alleviate the negative psychological, conducive to the resumption of the disease.

Proliferative and invasive effects of inhibitors of kinase 4(P15(ink4b) and P16(ink4a)/CDKN2) gene activation on RKO human colorectal cancer cells / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To explore the proliferation and invasive effects of inhibitors of kinase 4(INK4)(P15(ink4b) and P16(ink4a)/CDKN2) gene protein activation on RKO human colorectal cell in vivo and in vitro.</p><p><b>METHODS</b>RKO human colorectal cell line was exposed to the specific DNA methyltransferase inhibitor 5-Aza-CdR and INK4(P15(ink4b) and P16(ink4a)/CDKN2) protein expression was detected by Western blotting. Soft agar cloning experiment and Transwell chamber assay were used to detect the proliferative and invasive ability in vitro. Tumorigenicity in nude mice was analyzed in vivo.</p><p><b>RESULTS</b>INK4(P15(ink4b) and P16(ink4a)/CDKN2) protein expression of RKO human colorectal cells after exposure to 1×10(-7), 5×10(-7) and 1×10(-6) mol/L 5-Aza-CdR concentrations(A, B, C groups) were 1.13, 1.38, 1.92 folds and 1.11, 1.45, 2.14 folds compared to positive control group respectively. Soft agar cloning experiment showed the number of cell colony significantly decreased from 36.8±5.1(positive control group) to 32.4±7.2, 21.3±5.4 and 19.5±6.4 (3 experiment groups, all P<0.05) respectively. Transwell chamber assay showed that migrated cell number in positive control group(67.4±7.2) was significantly higher than those of 3 experimental groups(35.3±4.6, 29.5±7.3 and 25.3±6.2, respectively). The tumor volume of metastasis model in nude mice was inhibited in experimental groups, but not significantly lower compared to control group (P>0.05). There were significant differences of tumor weight and inhibition rate between control group and 3 experimental groups in nude mice respectively(all P<0.01).</p><p><b>CONCLUSION</b>INK4(P15(ink4b) and P16(ink4a)/CDKN2) protein activation can inhibit tumor proliferation, migration and suppress the tumor formation ability.</p>

Influence of finasteride on the expression of hypoxia-inducible factor 1 α and vascular endothelial growth factor in the tissue of prostate cancer / 中国基层医药

Objective To study the influence of finasteride on the expression of hypoxia-inducible factor 1 α(HIF-1 α) and vascular endothelial growth factor (VEGF) with prostate cancer (Pca).Methods The patients diagnosed of Pca were selected,and which treated with finasteride for up to six months were finasteride group and untreated with finasteride were control group.The expression of HIF-1 α,VEGF and microvessel density(MVD) was examined.Results The positive expression of HIF-1α,VEGF and MVD in the finasteride group were 55.0％,57.5％,(56.9 ± 13.8),respectively,and were lower than those of the control group [87.5 ％,97.5 ％,(64.2 ± 13.3)] (x2 =10.313,18.122,all P ＜ 0.01 ; t =2.391,P ＜ 0.05).In the pathological grade,advanced grade of the finasteride group was in 15 cases(37.5％),and 10 cases(25.0％) was in the control group,and the difference was statistically significant between the two groups(x2 =1.455,P ＞ 0.05).Conclusion Long-term treatment with finasteride can reduce expression of HIF-1α and VEGF with Pca,thus inhibit angiogenesis and tumor growth and metastasis.

Study on Grey model of electroencephalogram and features of driving fatigue / 生物医学工程学杂志

Grey system theory was applied in analysis of Electroencephalogram (EEG) to extract features of driving fatigue in this study. Model GM(1,1) was built for EEG collected during simulative driving experiments. At the same time, the data of steering wheel movements and subjective fatigue level were analyzed as reference. The results of experiments reveal that the co-deviation of Model GM(1,1) parameter a and b, cov(a,b), coincides with the standard deviation of steering wheel movements. This indicates that Grey system theory is effective for EEG analysis and the parameters of GM(1,1) can well reflect the change of driving fatigue.

Detrended fluctuation analysis of physiological parameters during sleep / 生物医学工程学杂志

Detrended fluctuation analysis (DFA) is fit for studies on the long-range exponential correlations of non-stationary time serial. In this paper, for elucidating the characteristics of different sleep stages, DFA is adopted to analyze the physiological data collected during sleep. The parameters such as electroencephalogram (EEG), R-R interval sequence and stroke volume (SV) are analyzed, and the scaling exponent a is calculated. The experimental results reveal that the values of a differ much in different sleep stages,that the rules of EEG and SV are alike, that alpha increases with the deepening of sleep, but in inverse for R-R interval sequence that alpha decreases with the deepening of sleep. These indicate that the method of DFA is practical in the analysis of physiological parameters.

Nonlinear analysis of multi-channel EEG and its application to mental workload detection / 生物医学工程学杂志

Mental workload research is important to people's health and work efficiency, Psychophysiological measures such as electroencephalography (EEG), ECG and respiration measures can be used to predict mental workload level. A Multi-channel phase-space reconstruction method is proposed in this paper which rearranges signal serials by the correlation coefficients and select time delay by signal determinism. The study of determinism and correlation dimension on simulative data exhibits a good performance. The result of EEG series shows a clearly consistency to workload level variety. The method is useful for multi-channel signals nonlinear analysis and mental workload detection.

Quasi-Newton iteration algorithm for ICA and its application in VEP feature extraction / 生物医学工程学杂志

Some noises still exist in the single-trial averaged visual evoked potentials (VEP), so further extraction of the above results is of significance. Independent component analysis (ICA)can separate the sources from their mixtures and make the output statistically as independent as possible; it can remove noises effectively. In this paper, the principle, experiment analyses and results of ICA based on quasi-Newton iteration rule for VEP feature extraction are introduced, It is compared with the fixed-point FastICA algorithm. The experiment results show that the provided algorithm may reinforce signals effectively and extract distinct P300 from the single-trial averaged VEP. It is of good applicability.

Resistant Genes of Beta-lactam Antibiotics from Clinical Isolates of Pseudomonas aeruginosa / 中华医院感染学杂志

OBJECTIVE To investigate ?-lactamase coding genes and oprD2 gene in multidrug-resistant Pseudomonas aeruginosa isolated.METHODS Polymerase chain reaction(PCR) was used to detect various ?-lactamase coding genes including TEM,SHV,OXA,PER,GES,IMP,VIM,plasmid type AmpC ?-lactamase DHA,MIR and oprD2 in 20 strains of P.aeruginosa.RESULTS The detection rate of ?-lactamase coding genes plasmid type AmpC,VIM,TEM,SHV,OXA and IMP were 15%,5%,15%,10%,10% and 5%,respectively,the loss rate of oprD2 was 25%,but PER and GES genes were negative.CONCLUSIONS The study indicated that the P.aeruginosa is carrying genes of DHA,TEM,SHV,OXA,VIM,IMP and being lost oprD2 gene,the latter is the essential resistance mechanism of P.aeruginosa to beta-lactam antibiotics in local area.

Analyzing sleep EEG using correlation dimension and approximate entropy / 生物医学工程学杂志

Correct sleep scoring is the base of sleep studying; nonlinear features of EEG can represent different sleep stages. In this paper, correlation dimension (D2) and approximate entropy (ApEn) of sleep EEG have been calculated. The statistical results reveal that: D2 does not come to be saturated when the embedding dimension increases, but the relative value of D2 can effectively distinguish different sleep stages. ApEn has the advantage of calculating simply, steady result and representing preferably different sleep stages. ApEn and the relative value of D2 reveal, from different point of view, the same rule about EEG (brain) complexity changing, that is, both complexity and its fluctuation are maximal in the subject's awake hour, are decreasing with the deepening of sleep, but the complexity in REM is about the level between S1 and S2.

Expressed prostatic secretion leukocyte count in the diagnosis of chronic prostatitis / 中华泌尿外科杂志

Objective To investigate the value of expressed prostatic secretion (EPS) leukocyte count in the diagnosis of chronic prostatitis. Methods EPS was obtained from 75 out patients with chronic prostatitis,pH and WBC per high power microscopic field being studied.Transabdominal prostatic ultrasonography was performed. Results Mean pH of the EPS was 6.9?0.4.pH of the EPS and the frequency of ultrasound abnomalities were significanly correlated to the leukocyte count in EPS.There was no correlation between age of symptom duration and the pH of EPS, frequency of ultrasound abnormalities or white blood cell counts in EPS. Conclusions In chronic prostatitis,the leukocyte count in EPS was positively correlated with the ultrasound abnomalities and the pH elevation of EPS.There was no correlation between age or symptom duration and pH of the EPS,frequency of ultrasound abnormalities or leukocyte count in the EPS.

The protective effects of OLA-PENa on experimental liver injury in rats / 中国药学杂志

To investigate the protective effects of OLA-PENa on experimental hepatic injury in rats and compare it with OLA in the dose-response relationships. METHODS: Biochemical indexes and histopathological examination of hepatic injury in rats caused by toxicant chemicals ［D-galactosamine (D-Galn) and CCl4］ were determined. RESULTS: OLA-PENa obviously inhibited the rising of serum alanine transaminase (ALT) caused by D-Galn and CCl4 and dramatically decreased liver fat storage as well in a dose-dependent manner. Histopathological examination showed that OLA-PENa can evidently alleviate the condition of the degeneration of hepatic cells and that of necrosis. CONCLUSION: The protective effects of OLA-PENa on experimental liver injury in rats, all the mentioned effects, are more powerful than those of OLA with the same dosage.

Hepatoid adenocarcinoma of the stomach: report of 3 cases and review of the literature in recent ten years / 中国癌症杂志

Purpose:To analyze the clinical manifestations, so the diagnosis of hepatoid adenocarcinoma of the stomach (HAS) may be made and treatment instituted as early as possible. Methods:A total of 65 cases of HAS reported in the literature in recent ten years reviewed.Results:①Clinical symptoms and signs: The incidence of HAS in male was higher than that in female (6.2:1).Upper abdominal discomfort and physical signs of mass bclow the sternum were most commonly seen.②Diagnosis: The serum content of AFP were discombfort high level with a positive rate of 66.7%(22/33). The positive rate of AFP expression with immunohistochemical method was 94.5%(52/55).The percentage of liver metastasis detected by US B or CT was 20.5%(9/44) preoperation, and mostly seen at the gastric sinus was ahout 63.3%(19/30). The final diagnosis of HAS depends on gastrocopy and pathology. ③Treatment and Prognosis: The possibility of radical operation(D 2 ) is only 17.9%(5/28). The death ratio within one year was 56.3%(18/32).Conclusions: HAS is primarily a gastric carcinoma. The serum AFP levels are help fulin the diagnosis and differential diagnosis of HAS. The diagnostic evidence of HAS is dependant on its specific forms. The prognosis is often poor because of frequent liver metastasis.

Trans-Lymphatic Infusion of Immunochemical Drugs in the Treatment of Lymphatic Metastases / 介入放射学杂志

Purpose:To investigate the effectiveness of intralymphatic infusion of anti- cancer agents and cytokines in the treatmrnt of malignancy.Materials anti methods:23 patients suffering from advanced metastatic cancers anti 2 primary lymphomas,unresponsble to the standard therapies or intra-arterial chemotherapy,were treated with lymphatic injections of an- ticancer ddrugs or combiation with biochemotherapy.Results:Follow-up study about one month after the therapy,comparing with findings on lymthatic radiographies anti computed tomographic scans,revealed decrease of lymphnodes in size in 23 cases.Conclusion:This therapeutic ap- proach proved to be an effective and safe method for the palliative treatment of advanced lym- phatic metastases and lymphomas.The procedure was feasible without serious compllications.