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Strengthening the supervision over the use of large-scale medical equipment is an effective means to improve the efficiency of equipment use and the quality of medical services, and it is an important part of promoting the construction of the Healthy China and the development of health undertakings. Through four stages of preliminary demand investigation, intelligent collection of data, intelligent analysis and evaluation, and continuous improvement, a large-scale medical equipment intelligent management platform was built in our hospital. Real-time data collection, interconnection, analysis and evaluation were achieved, which could help the use and supervision, improve efficiency and effectiveness, and optimize the evaluation system.
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Objective To study the tropism of umbilical cord mesenchymal stem cells (UCMSCs) to glioma stem cells and their mechanism.Methods The isolated humanized UCMSCs were cultured and identified.The glioma stem cells were induced to remain at the resting, proliferation or differentiation states by soft or hard agar combined with stemness maintenance factors (epidermal growth factor [EGF] and basic fibroblast growth factor [bFGF]): soft agar+EGF+bFGF group, hard agar+EGF+bFGF group, and hard agar group;clone spheres of the stem cells were cultured for 7 d, and then, the growth curve of clone spheres diameters was drawn;flow cytometry was used to detect the cell cycle of the stem cells 72 h after culture;CD133, Ki67 and GFAP expressions in the clone spheres of stem cells in the three groups were observed by immunofluorescent staining;the tropism aptitude of UCMSCs to glioma stem cells at different states was detected by Transwell dual chamber culture system;the protein expression levels of stem cell factor (SCF), stromal-derived factor (SDF)-1 and vascular endothelial growth factor (VEGF) in glioma stem cells were monitored by enzyme immunoassay (ELISA);Western blotting was used to measure the protein expression levels of c-Kit, C-X-C chemokine receptor type 4 (CXCR-4) and VEGF receptor in UCMSCs.Results The diameters of clone spheres in the hard agar+EGF+bFGF group and hard agar group were significantly larger than those in the soft agar+EGF+bFGF group on the second and 7th d of culture (P＜0.05);cell cycle analysis indicated that glioma stem cells in the soft agar+EGF+bFGF group, hard agar+EGF+bFGF group, and hard agar group were successfully remained at the resting, proliferation or differentiation states;high CD133 expression ([95.79±5.31] ％) was noted in the cells of soft agar+EGF+bFGF group, high Ki67 and GFAP expressions ([89.39±7.45 and 63.49±16.54] ％) were noted in the cells of hard agar+EGF+bFGF group, and high GFAP expression ([97.36±3.48] ％) was noted in the cells of hard agar group;Transwell assay indicated that the migration percentage of cells from the hard agar+EGF+bFGF group was significantly higher than that in the soft agar+EGF+bFGF group, hard agar group and two control groups (P＜0.05).higher protein expression levels of SCF, SDF-1 and VEGF in the hard agar+EGF+bFGF group were noted as compared with those in the soft agar+EGF+bFGF group and hard agar group (P＜0.05).UCMSCs were induced to express significantly higher levels of c-Kit, CXCR4 and VEGF receptor in the hard agar+EGF+bFGF group as compared with those in the soft agar+EGF+bFGF group and hard agar group (P＜0.05).Conclusion SCF/c-Kit, SDF-1/CXCR4 and VEGF/VEGFR axis expression defects might be the potential mechanism of the migration defect of UCMSCs to glioma stem cells at the resting state.
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Objective To explore the gene transfer characteristics and different cell tropism of the recombinant adenovirus serotype 5 (rAd5) driven by murine cytomegalovirus (mCMV) immediate-early promoter in the hippocampus of adult mouse,and provide a basis for adenovirus-mediated transgene research in the hippocampus of mouse in the future.Methods Forty-two male healthy adult C57BL/6 mice were injected with the titre and volume matched rAd5 vectors which contained enhanced green fluorescent protein (EGFP) sequences as a reporter gene.Then animals (n=6 for each point) were killed 1,3,7,14,30,45,60 days after injection.The coronal cryosections of brains were processed,and EGFP expression was observed with fluorescence microscopy.Double-label immunofluorescence method was used to observe the EGFP expression in different types of cells to identify the cell tropism in the hippocampus.Results The expression of EGFP carried by rAd5 vectors was detected on the first day after the transfection,with peak expression between days 3 and 45,while EGFP activity decreased sharply 60 days after transfection.EGFP highly expressed in the dentate subgranular zone (SGZ) of the hippocampus but rarely in the granule cell layer (GCL) of the dentate gyrus.Moreover,no EGFP was detected in the pyramidal cell layers of CA1 to CA3 area.The counterstaining showed that rAd5 mainly transduced neuronal precursor cells in the SGZ of the hippocampus and rarely transduced neurons in the GCL.Conclusion The rAd5-mediated gene transfer is rapid and efficient and can keep for a long time in suitable titre; moreover,rAd5 has a highly specific tropism with the neuronal precursor cells in the SGZ of the hippocampus in mice.
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Current pathological diagnosis of glioma grades is difficult because of the existence of heterogeneity. The development of proteomics can be a good tool for high-flux screening protein marks related to glioma. The combination of multiple proteins may enhance detecting specificity and sensitivity for ghoma diagno-sis. And the proteomics may offer chances for glioma grading in molecular level,objectively evaluating biological characteristics of different tumor types ,judging prognosis,and studying new therapy drugs.
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Objective To locally inject human umbilical cord blood (HUCB) mesenchymal stem cells (MSCs) to rat traumatic brain injury (TBI) model to investigate expression of neural markers and neurological functional improvement. Methods HUCB-MSCs were labeled by bis-benzimide for over 24 hours and stereotactically transplanted into the brain of the rats. All rats were divided into four groups, ie, sham injury group, TBI group, control (TBI + PBS) group and treatment (TBI + MSCs) group, Im-munohistochemical methods and immanofluorescence staining were used to observe the survival, migration and differentiation of the transplanted cells. The neurological functional improvement was evaluated by u-sing the neurological severity score (NSS). Results There existed a large number of MSCs survived in local region of the brain that received transplants, when some MSCs differentiated into neurons or astro-cytes and expressed the neurocyte markers including NSE and GFAP around the grafted site. Treatment group had significantly improved scores compared with sham injury group, TBI group and control group. Conclusions HUCB-MSCs transplantation can potentially improve neurological functional after TBI and may be a good alternative to bone marrow cells for stem cell transplantation or cell therapy.
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Objective To judge injury severity of severe traumatic brain injury (sTBI) by using surface enhanced laser desorption-ionization (SELDI) protein chip technique. Methods Serum sam-ples from sTBI patients were used to detect expression of differential proteins by protein chip CM10 and SELDI to analyze the correlation between expression peak intensity and GCS. Results We obtained 101 protein peaks, with statistical difference upon expression of 27 protein peaks, when negative correla-tion was found between two peaks ( m/z 4 972 and m/z 5 322 ) and GCS score and positive correlation be-tween six peaks (m/z 3 941, m/z 4 295, m/z 8 714, m/z 8 792, m/z 14 020 and m/z 28 148) and GCS score. Conclusion SELDI protein chip technique may become a new and objective detection method in judging injury severity of sTBI.
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Objective To study the changes of proteome expression in brain tissues from rats with severe traumatic brain injury(sTBI). Methods Total protein of brain tissues were obtained at days 3,7 and 14 for two-dimensional gel electrophoresis to screen and identify differential protein spots.Results We screened 17 differential protein spots that were involved in cellular metabolism,stress and inflammatory reaction. Conclusion Some differential proteins involved in sTBI can be found by twodimensional gel electrophoresis.
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<p><b>OBJECTIVE</b>The fibrogenicity of fur dust was studied in rat lung tissues.</p><p><b>METHODS</b>Intratracheal instillation of fur dust, morphologic examination of lungs and analysis of collagen content were performed in Wistar rats.</p><p><b>RESULTS</b>Morphologic examination revealed that the earliest changes consisted of alveolar edema, increased numbers of intraalveolar macrophages, and marked thickening of interalveolar septa with mixed cellular infiltrate. After sixth months, there was moderate thickening of the alveolar walls and the peribronchioli. After 12 months, interstitial positive fibrosis of the alveolar wall and the peribronchioli were weakly seen. In the carding dust group (silica content 17.6%), interstitial nodules were observed composed of fibroblasts, reticular fibers, and collagen fibers. Electron microscopic examination also showed that alveolar walls became thickened and collagen fiber bundles were seen around bronchioles and small vessels in the carding groups after 12 months. At all stages of analysis, the collagen content in lungs of the fur dust groups was significantly higher than that of the control group.</p><p><b>CONCLUSIONS</b>Our study suggested that fur dust might induce weak interstitial fibrosis in the lung.</p>
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Objective: The fibrogenicity of fur dust was studied in rat lung tissues. Methods: Intratracheal instillation of fur dust, morphologic examination of lungs and analysis of collagen content were performed in Wistar rats. Results: Morphologic examination revealed that the earliest changes consisted of alveolar edema, increased numbers of intraalveolar macrophages, and marked thickening of interalveolar septa with mixed cellular infiltrate. After sixth months, there was moderate thickening of the alveolar walls and the peribronchioli. After 12 months, interstitial positive fibrosis of the alveolar wall and the peribronchioli were weakly seen. In the carding dust group (silica content 17.6%), interstitial nodules were observed composed of fibroblasts, reticular fibers, and collagen fibers. Electron microscopic examination also showed that alveolar walls became thickened and collagen fiber bundles were seen around bronchioles and small vessels in the carding groups after 12 months. At all stages of analysis, the collagen content in lungs of the fur dust groups was significantly higher than that of the control group. Conclusions: Our study suggested that fur dust might induce weak interstitial fibrosis in the lung.
Sujet(s)
Poussière , CollagèneRÉSUMÉ
<p><b>OBJECTIVE</b>To study the respiratory system injury in fur processing environment.</p><p><b>METHODS</b>Environmental fungal survey was conducted in the fur processing procedures. Investigation of respiratory symptoms and chest X-ray examination were also carried out in 138 fur processing workers and 40 control workers. At the same time, the serum antibodies to fungi were analyzed by ELISA.</p><p><b>RESULTS</b>Fungal number(629-3,681 cfu/m3) in fur processing procedures was much higher than those in the control environment. Cladosporium and Alternaria were the leading strains of fungi in fur processing procedures. The rates of respiratory symptoms(cough, sputum, chest tightness, dyspnea, and fever) in fur processing workers were higher than those in the control workers. The rates of the symptoms in female workers were 37.9%, 28.4%, 10.5%, 22.1%, 4.2%, respectively. Abnormalities of chest X ray were found in 7 workers. The serum antibodies to Cladosporium and Alternaria(A450 nm 0.631, 0.724, respectively) in fur workers were significantly higher than those in the control workers(P < 0.05). The positive rates of the antibodies to Cladosporium and Alternaria(44.2%, 42.8%) were significantly higher than those in the control workers(P < 0.01).</p><p><b>CONCLUSION</b>Cladosporium and Alternaria may be the pathogens of occupational respiratory diseases in fur processing workers.</p>