¿Cuándo el patólogo debe solicitar inmunomarcación con B-Catenina?: una revisión de la literatura a propósito de dos casos / When should the pathologist request immunostaining with B-catenin?: a review of the literature on two cases

For more than 20 years, immunohistochemistry has represented an auxiliary test of great relevance to support pathological work, however, it should be noted that the pillar of diagnosis continues and will continue to be the classic morphological description based on hematoxylin eosin and the trained eye of the specialist. In neoplastic pathologies, whether benign or malignant, it is becoming increasingly necessary to incorporate new tissue biomarkers that help objectify or confirm the diagnosis of each patient, in order to provide better treatment or a more precise diagnosis about the biological nature of their illness. In this line, there has been intense research in relation to the participation of the Wnt/ß-catenin pathway in the development of various types of tumors, including colon adenocarcinoma, some pancreatic neoplasms and even some tumors of mesenchymal origin, as will be seen. in this work. In this context and based on two clinical cases of special interest, we have prepared a brief review of the literature considering the biological aspects of ß-catenin, tumors where there is currently a true relative consensus that its immunolabeling offers a real contribution to the confirmation of the entity and finally a limited exposition regarding the future of this biomarker in the pathology discipline.

Desde hace más de 20 años la inmunohistoquímica ha representado una prueba auxiliar de gran relevancia para apoyar el trabajo anatomopatológico, no obstante, cabe señalar que, aún el pilar del diagnóstico sigue y seguirá siendo la descripción morfológica clásica basada en hematoxilina eosina y el ojo entrenado del especialista. En las patologías neoplásicas, ya sea benignas, como malignas, se hace cada vez más necesario la incorporación de nuevos biomarcadores tisulares que ayuden a objetivar o confirmar el diagnóstico de cada paciente, con objeto de entregar un mejor tratamiento o un diagnóstico más preciso de la naturaleza biológica de su enfermedad. En esta línea, ha habido intensa investigación en relación con la participación de la vía Wnt/ß-catenina en el desarrollo de varios tipos de cáncer, entre ellos el adenocarcinoma de colon, algunas neoplasias pancreáticas e incluso algunos tumores de origen mesenquimal como se verá en este trabajo. En este contexto y partir de dos casos clínicos de especial interés, hemos preparado una breve revisión de la literatura considerando los aspectos biológicos de la ß-catenina, los tumores donde en la actualidad existe verdadero consenso de que su inmunomarcación ofrece un aporte real a la confirmación de la entidad y finalmente una exposición acotada respecto al futuro de este biomarcador en la disciplina de la anatomía patológica.

DNA replication and sister chromatid cohesion 1 promotes breast carcinoma progression by modulating the Wnt/β-catenin signaling and p53 protein

The objective of this study is to assess the prognostic and functional role of DSCC1 in breast carcinoma, aswell as the potential mechanism. Based upon the TCGA data, the expression pattern and prognostic value ofDSCC1 in breast carcinoma was evaluated. The mRNA and protein levels of molecules were determined usingqRT-PCR and Western blot. In vitro functional role of DSCC1 in tumor cells was determined using cellcounting kit 8, clone formation, and Transwell assays. Gene set enrichment analysis (GSEA) was conducted todetermine DSCC1 related gene sets, which are further confirmed by Western blot. The results showed thatDSCC1 is overexpressed in breast carcinoma tissues and its high expression was linked to shorter overallsurvival. Overexpression of DSCC1 facilitated the proliferation, invasion and migration of breast carcinomacells, while knockdown of DSCC1 showed opposite outcomes. GSEA showed that high DSCC1 expressionhad a positive correlation with p53, and Wnt signaling-related molecules. Western blot showed that silencingDSCC1 increased the levels of p53 and p-b-catenin, whereas decreased p-GSK-3b and cyclin D1 expression.These observations illustrate that DSCC1 emerges a well value on the diagnosis and prognosis of breastcarcinoma, and facilitates the progression of breast carcinoma partly by activating Wnt/b-catenin signaling andinhibiting p53.

miR-425-5p suppresses tumorigenesis and DDP resistance in human-prostate cancer by targeting GSK3 β and inactivating the Wnt/β-catenin signaling pathway

In previous studies, we found interferon-a (IFN-a) could reduce protein levels of p11, 5-hydroxytryptamine receptor 1b(5-HT1b) and 5-hydroxytryptamine receptor 4 (5-HT4), but does not influence their messenger RNA levels in SH-sy5ycells. Thus, we investigated the post-transcriptional modulation of these molecules by IFN-a. SH-sy5y cells were treatedwith IFN-a, NH4Cl or MG132 alone or in combination, and then the protein levels of p11, 5-HT1b and 5-HT4 wereanalyzed by western blots. The regulatory effects of p11 on 5-HT1b and 5-HT4 were also determined in p11 knock-downcells. NH4Cl but not MG132 could reverse the protein level of p11 in IFN-a-treated SH-sy5y cells. MG132 could recoverthe protein levels of 5-HT1b and 5-HT4 in p11 knock-down cells. The down-regulation effects of IFN-a on p11, 5-HT1band 5-HT4 were associated with the lysosome and ubiquitin–proteasome-mediated pathways. p11 was identified as a potentregulator to modulate the ubiquitination of 5-HT1b and 5-HT4. Therefore, it could be potential target therapies in IFN-ainduced depression.

Study of E-Cadherin / 中国矫形外科杂志

E-Cadherin is one of the Ca2+-dependent cell adhesion molecule family.It plays an important role in maintaining the normal morphology and structural integrity of epithelial cells.This review aims to clarify the structure,function and regulation etc.of E-Cadherin.