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1.
Yao Xue Xue Bao ; (12): 198-201, 2024.
Article de Chinois | WPRIM | ID: wpr-1005430

RÉSUMÉ

To establish and optimize a method for the detection of recombinant human midkine (rhMK) activity and verify its methodology, cell counting kit-8 (cck-8) method was used to measure the proliferation activity of rat knee chondrocytes. The specificity, accuracy, precision, linearity and robustness of the method were also verified in this study. The established method was proven to have good specificity because the buffer of rhMK and recombinant human interleukin-1 receptor antagonist have no obvious active effect; the recoveries of the samples with relative activities of 50%, 75%, 100%, 125%, 150% were in the range of 80.0% to 124.0% by statistical analysis, the relative standard deviations (RSD) of relative potency were all within 20%, the linear correlation coefficient, R2 ≥ 0.98, suggesting that the accuracy, precision and linearity of the method were good; the robustness correlation coefficient, R2 ≥ 0.92 and the ratio of maximum to minimum of sigmoidal dose-response were no less than 1.5, indicating that robustness of the methods was good. In conclusion, a bioactivity measurement method for rhMK was established and fully validated in this study and it provides a reliable method for the bioactivity analysis of rhMK routine samples during the development. This study was approved by the Animal Care and Use Committee of Shanghai Model Organisms Center, Inc. (approval number: 2019-0008-06).

2.
Article de Chinois | WPRIM | ID: wpr-1021295

RÉSUMÉ

BACKGROUND:Bioactive glass bone repair material has bone-bonding ability,bone induction ability and bone conduction characteristics.However,the performance of bioactive glass does not meet the requirements of clinical application,and the addition of boron is expected to improve the performance of bioactive glass. OBJECTIVE:To study the effect of different contents of B2O3 replacing SiO2 on the mechanical properties and bioactivity of bioactive glass. METHODS:Based on bioactive glass containing phosphorus nitrogen and oxygen(composition:SiO2-CaO-ZnO-Na2O-Si3N4-P2O5),B2O3 was used to partially replace the SiO2.The basic glass containing B2O3 with a mass fraction of 0%(group A),5%(group B),10%(group C),and 15%(group D)was fired using the high-temperature melting method(the total mass fraction of SiO2 and B2O3 in the basic broken glass was 41%).Porous bioactive glass scaffolds were fabricated by the organic foam impregnation method.Uniaxial compression and three-point bending method of universal mechanical testing machine were used to test mechanical properties.Four groups of scaffolds were immersed in simulated body fluids to detect the degradation performance of scaffolds.Scanning electron microscopy was used to observe the morphological changes of scaffolds before and after soaking.X-ray diffraction was used to analyze the phase composition of scaffolds before and after soaking. RESULTS AND CONCLUSION:(1)With the increase of the mass fraction of B2O3,the compressive strength and bending strength of the porous bioactive glass scaffold increased,and there was a significant difference between the compressive strength and bending strength of the four groups(P≤0.05).(2)After soaking in simulated body fluids,the porous bioactive glass scaffolds degraded gradually with the extension of time.At the same soaking time point,the degradation rate of the scaffolds was accelerated with the increase of the mass fraction of B2O3,and the compressive strength and bending strength of the scaffolds in the four groups were significantly different(P≤0.05).(3)Scanning electron microscopy after soaking in simulated body fluids showed that a large number of granular materials were deposited on the surface of group A and group B after soaking for 1 day.After 3 days,the granular materials on the surface fused with each other to form film-like deposits.After 7 days,the films on the surface fused with each other to form pieces,basically covering the entire surface of the specimen.After soaking for 1 day,film-like material deposition was formed on the surface of group C,and after 3 days,the films on the surface were fused into pieces,basically covering the whole surface of the specimen.After soaking for 1 day in group D,flake material covering the whole surface of the specimen could be seen.(4)X-ray diffraction analysis after 1 day of immersion in simulated body fluids showed that the deposits on the surface of the four groups of scaffolds were crystallized hydroxyapatite.(5)B2O3 replacement of SiO2 can enhance the mechanical properties,degradation properties and in vitro mineralization activity of porous bioactive glass scaffolds.

3.
Yao Xue Xue Bao ; (12): 543-553, 2024.
Article de Chinois | WPRIM | ID: wpr-1016618

RÉSUMÉ

Currently, clinically used drugs for the treatment of gout inflammation, such as colchicine, nonsteroidal anti-inflammatory drugs, and glucocorticoids, can only relieve the pain of joint inflammation and have severe hepatorenal toxicity and multiple organ adverse reactions. The NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome is a key complex that induces the onset of gout inflammation and has become a crucial target in the development of anti-gout drugs. This article reviews the research progress of anti-gout small molecules targeting the NLRP3 inflammasome and their bioactivity evaluation methods in the past five years, in order to provide information for the development of specific drugs for the treatment of gout inflammation.

4.
São José dos Campos; s.n; 2024. 143 p. ilus, tab.
Thèse de Portugais | LILACS, BBO | ID: biblio-1570225

RÉSUMÉ

O estudo avaliou o efeito da adição de partículas bioativas ao gel clareador (peróxido de hidrogênio 35%, técnica de consultório), quanto ao clareamento e sobre a estrutura dental, além da resistência à desmineralização. O objetivo da primeira parte do estudo foi avaliar géis, com diferentes partículas bioativas e concentrações, para o potencial clareador e às alterações da estrutura dental. O objetivo da segunda parte foi avaliar géis, com diferentes partículas bioativas e concentrações, perante a suscetibilidade do esmalte ao protocolo cariogênico e ao desgaste dental erosivo. Espécimes de incisivos bovinos foram avaliados na primeira parte por análise da cor, microdureza Knoop e rugosidade. Os grupos foram controle negativo (CN), controle comercial (CCWhiteness HP), controle experimental (CE-gel sem partícula), controle positivo (CPsolução de peróxido), e os grupos experimentais onde as partículas bioativas S-PRG e 45S5 foram utilizadas nas concentrações de 5, 10, 15 e 20 %. Na segunda parte foram avaliados por microradiografia para a alteração da densidade mineral e profundidade da lesão perante o protocolo cariogênico e pela perfilometria para o desgaste dental erosivo. Os grupos foram CN, CC, CE, controle fluoreto (CF), e os grupos experimentais onde as partículas bioativas S-PRG e 45S5 foram utilizados nas concentrações de 15 e 20 % apenas. Os dados foram analisados estatisticamente para verificar a diferença entre os grupos (α=5%). Para a mudança de cor (∆E*00) foi observada diferença apenas para o fator partícula, S-PRG-3,37(0,48)a e 45S5-3,67(0,76)b. Todos os grupos experimentais foram estatisticamente iguais o CP e diferentes do CN. Para a microdureza (KNH%) foi observada diferença para a interação dos fatores, sendo o S-PRG 20% o grupo com maior aumento da microdureza. Todos os grupos experimentais foram diferentes e maiores que CP, CC e CE. Não foram observadas diferenças de rugosidade entre os grupos. Para a alteração da densidade mineral foi observada diferença para a interação dos fatores e tempo. Os grupos S-PRG 15% e 20% foram os grupos que apresentaram a menor alteração da densidade mineral em todos os tempos testados. Aos 14 e 21 dias o SPRG 20% foi diferente do CN e semelhante ao CF. Para a profundidade da lesão foram observadas diferenças estatísticas para o fator partícula e tempo, S-PRG206,8(59,2)a e 45S5 230,0(63,7)b, 7 dias-148,9(19,7)a, 14 dias-217,7(22,5)b e 21 dias288,7(30,7)c. Aos 14 e 21 dias o S-PRG 20% foi diferente do CN e semelhante ao CF. Para o desgaste foi observada diferença apenas para o tempo, 5 dias-1,2(0,2)a e 10 dias-2,3(0,3)b. Conclui-se que: a adição de partículas bioativas não reduziu a efetividade clareadora, contribuiu para o aumento da microdureza e não alterou a rugosidade. Além disso, o grupo S-PRG com 20% teve suscetibilidade à desmineralização por cárie semelhante ao grupo controle fluoreto e nenhum grupo alterou a suscetibilidade ao desgaste dental erosivo (AU)


The study evaluated the consequences of bioparticle addition on tooth bleaching gel (hydrogen peroxide 35%, in-office technique), for its bleaching effect and changes on tooth structure, also for resistance against demineralization. The aim of the first part of the study was to evaluate bleaching gels containing different bioactive particles and concentrations over the bleaching potential and possible dental structural changes. The objective of the second part was to evaluate bleaching gels containing different bioactive particles and concentrations to assess enamel susceptibility to cariogenic and erosive tooth wear protocols after bleaching treatments. Bovine incisors specimens were used in the first part for analysis of color, Knoop microhardness and roughness. The groups were negative control (NC), commercial control (CCWhiteness HP), experimental control (EC-gel without particles), positive control (PCperoxide solution), and experimental groups were the bioactive particles S-PRG and 45S5 used in concentrations of 5, 10, 15, and 20 %. In the second part, specimens were evaluated using advanced transverse microradiography to assess the change in mineral density and lesion depth in response to the cariogenic protocol, and profilometry to measure wear in response to the erosive tooth wear protocol. The groups were NC, CC, EC, fluoride control (FC), and experimental groups were the bioactive particles S-PRG and 45S5 used only in concentrations of 15 and 20 %. The data were statistically analyzed to verify the differences between the groups (α=5%). For the color change (∆E*00), a difference was observed only for particle, S-PRG3,37(0,48)a and 45S5-3,67(0,76)b. All experimental groups were statistically similar to PC and different from NC. For the microhardness (KNH%), difference was observed for factors interaction, S-PRG 20% was the group with higher hardness increase. All experimental groups were different and higher than PC, CC, and EC. No roughness differences were observed between groups. For the mineral density alteration differences were observed for factors interaction and time. S-PRG 15% and 20% presented the lower mineral density change for all measurements times. On 14 and 21 days S-PRG 20% was different from NC and similar to FC. For lesion depth differences were observed for factor particle and time, S-PRG-206,8(59,2)a and 45S5- 230,0(63,7)b, 7 days-148,9(19,7)a, 14 days-217,7(22,5)b and 21 days-288,7(30,7)c. On 14 and 21 days S-PRG 20% was different from NC and similar to FC. For wear the only difference was for time, 5 days-1,2(0,2)a and 10 days-2,3(0,3)b. It was concluded that the addition of bioactive particles did not reduce the bleaching effectiveness, it contributed to microhardness increase and did not change roughness. Furthermore, the S-PRG group with 20% susceptibility to cariogenic demineralization was similar to the fluoride control group, and no group showed a change in susceptibility to erosive tooth wear (AU)


Sujet(s)
Blanchiment dentaire , Déminéralisation , Couleur , Usure dentaire
5.
Braz. dent. j ; Braz. dent. j;34(1): 29-38, Jan.-Feb. 2023. tab, graf
Article de Anglais | LILACS-Express | LILACS, BBO | ID: biblio-1420580

RÉSUMÉ

Abstract The aim of this study was to evaluate the physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic material, Bio-C Repair (Angelus), in comparison with White MTA (Angelus) and Biodentine (Septodont). The physicochemical properties of setting time, radiopacity, pH, solubility, dimensional and volumetric changes were evaluated. Biocompatibility and bioactivity were assessed in Saos-2 osteoblast cell cultures by the MTT assay 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), Alizarin Red (ARS), and cell migration tests. Statistical analysis was performed by ANOVA, Tukey or Bonferroni tests (α = 0.05). Bio-C Repair had the longest setting time (p < 0.05), but radiopacity and solubility were accordance with the ISO 6876/2012 standards, besides linear expansion. Bio-C Repair and MTA had similar volumetric change (p > 0.05); lower than Biodentine (p < 0.05). All the materials evaluated had an alkaline pH. Bio-C Repair was cytocompatible and promoted mineralized nodule deposition in 21 days and cell migration in 3 days. In conclusion, Bio-C Repair had adequate radiopacity above 3mm Al, solubility less than 3%, dimensional expansion, and low volumetric change. In addition, Bio-C Repair promoted an alkaline pH and presented bioactivity and biocompatibility similar to MTA and Biodentine, showing potential for use as a repair material.


Resumo O objetivo deste estudo foi avaliar as propriedades físico-químicas, citotoxicidade e bioatividade de um novo material biocerâmico pronto para uso, Bio-C Repair (Angelus), em comparação com MTA Branco (Angelus) e Biodentine (Septodont). Foram avaliadas as propriedades físico-químicas de tempo de presa, radiopacidade, pH, solubilidade, alterações dimensionais e volumétricas. A biocompatibilidade, bioatividade e capacidade de reparo foram avaliadas em culturas de células de osteoblastos Saos-2 pelo ensaio MTT brometo de 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazólio), vermelho neutro (NR), vermelho de alizarina ( ARS) e testes de migração celular. A análise estatística foi realizada pelos testes ANOVA, Tukey ou Bonferroni (α = 0,05). Bio-C Repair apresentou o maior tempo de presa (p < 0,05), mas radiopacidade e solubilidade de acordo com as normas ISO 6876/2012, além de expansão linear. Bio-C Repair e MTA tiveram variação volumétrica semelhante (p > 0,05), menor que Biodentine (p < 0,05). Todos os materiais avaliados apresentaram pH alcalino. Bio-C Repair foi citocompatível, além de promover deposição de nódulos mineralizados em 21 dias e migração celular em 3 dias. Em conclusão, o Bio-C Repair apresentou radiopacidade adequada acima de 3mmAl, solubilidade menor que 3%, expansão dimensional e baixa perda volumétrica.. Além disso, o Bio-C Repair promoveu um pH alcalino e apresentou bioatividade e biocompatibilidade semelhantes ao MTA e Biodentine, mostrando potencial para uso como material reparador

6.
Article de Anglais | WPRIM | ID: wpr-971670

RÉSUMÉ

The fungus Xylaria sp. KYJ-15 was isolated from Illigera celebica. Based on the one strain many compounds (OSMAC) strategy, the strain was fermented on potato and rice solid media, respectively. As a result, two novel steroids, xylarsteroids A (1) and B (2), which are the first examples of C28-steroid with an unusual β- and γ-lactone ring, respectively, along with two new dihydroisocoumarin glycosides, xylarglycosides A (3) and B (4), were identified. Their structures were elucidated by spectroscopic methods, X-ray diffraction and electronic circular dichroism (ECD) experiments. All isolated compounds were evaluated for cytotoxicity, DPPH radical scavenging activity, acetylcholinesterase inhibitory and antimicrobial effect. Compound 1 exhibited potent AChE inhibitory activity with an IC50 value of 2.61 ± 0.05 μmol·L-1. The β-lactone ring unit of 1 is critical for its AChE inhibitory activity. The finding was further confirmed through exploring the interaction of 1 with AChE by molecular docking. In addition, both compounds 1 and 2 exhibited obvious antibacterial activity against Bacillus subtilis with a minimum inhibitory concentration (MIC) of 2 μg·mL-1. Compounds 3 and 4 exhibited antibacterial activities against Staphylococcus aureus with MICs of 4 and 2 μg·mL-1, respectively, which also exhibited DPPH radical scavenging activity comparable to the positive control with IC50 values of 9.2 ± 0.03 and 13.3 ± 0.01 μmol·L-1, respectively.


Sujet(s)
Humains , Acetylcholinesterase , Simulation de docking moléculaire , Antibactériens , Hétérosides , Lactones , Douleur
7.
Yao Xue Xue Bao ; (12): 773-778, 2023.
Article de Chinois | WPRIM | ID: wpr-965638

RÉSUMÉ

In this study, we established a novel bioassay to determine the activity of polyethylene glycolated recombinant human growth hormone (PEG-rhGH) using Nb2-11 cells. We performed experimental condition optimization and methodological verification, and then detected the relative potency of PEG-rhGH products using this method. We demonstrated that the bioactivity of PEG-rhGH in promoting Nb2-11 cell proliferation displays a dose-response relationship, which conformed to the four-parameter model. Using PEG-rhGH reference as a control, we analyzed the relative potency of six batches of PEG-rhGH products, as well as linearity, regression and parallelism of the obtained curves. The relative potency of six batches of PEG-rhGH products was 95% to 105%. These results implied that the new bioassay established may be employed in quality control of PEG-rhGH products.

8.
Chinese Herbal Medicines ; (4): 383-390, 2023.
Article de Anglais | WPRIM | ID: wpr-982509

RÉSUMÉ

Edible bird's nest (EBN) is a kind of natural invigorant with a long history of consumption in Asia, especially in China. EBN is formed by mixing the saliva of swiftlets (Aerodramus) with feathers and other components during the breeding season. Proteins are the most important nutrient in EBN. By studying proteins in EBN, we can not only elucidate their components at the molecular level, but also study their bioactivities. Therefore, it is of great significance to study the proteins in EBN. Previous research on the proteins in EBN was preliminary and cursory, and no one has summarized and analyzed the proteins in EBN and correlated the bioactivities of these proteins with the biological functions of EBN. This article focused on the proteins in EBN, listed the proteins identified in different proteomic studies, and introduced the sources, structures and bioactivities of the most frequently identified proteins, including acidic mammalian chitinase, lysyl oxidase homolog 3, mucin-5AC, ovoinhibitor, nucleobindin-2, calcium-binding protein (MW: 4.5 × 104) and glucose-regulated protein (MW: 7.8 × 104). The properties of these proteins are closely related to the bioactivities of EBN. Therefore, this article can provide inspiration for further research on the efficacy of EBN.

9.
Article de Chinois | WPRIM | ID: wpr-1019755

RÉSUMÉ

Systematic research on the quality evaluation methods of Chinese medicinal materials is an intrinsic requirement,which is beneficial to the clinical application of traditional Chinese medicine and the sound development of traditional Chinese medicinal industry.The intrinsic quality evaluation methods of Chinese medicinal materials have developed from chemical fingerprint to quality marker of Chinese medicinal materials,and it represents that the evaluation mode has developed from chemical component as research index to the combination of chemical component and pharmacodynamic components,chemical component and biological activity.The extrinsic character evaluation method of Chinese medicinal materials from"Quality Evaluation Through Morphological Identification"to the application of intelligent sensory technology make up for the accumulation of objective data.The trend of quality evaluation of the intrinsic quality and extrinsic traits of Chinese herbal medicines conform to the innovation strategy called integrated quality control of TCMs.This paper reviews the development and evolution of the research on the intrinsic quality and extrinsic traits of Chinese herbal medicines,systematically expounds the research status and development trend of"intrinsic and extrinsic combination",and facilitate the establishment of quality evaluation system of Chinese herbal medicines with standardization and informatization characteristics in accordance with the theory of traditional Chinese medicine.

10.
Chinese Journal of Biologicals ; (12): 800-804, 2023.
Article de Chinois | WPRIM | ID: wpr-996487

RÉSUMÉ

@#Objective To extract the total protein of K326 tobacco leaves with high expression of Nicotiana alata defensin 1(NaD1) gene and analyze its bioactivity.Methods Total proteins were extracted from Nicotiana alata flowers,wild type(WT) and K326 tobacco leaves(transgenic) with high expression of NaD1 gene,and determined for the concentrations by Bardford method,while for the antibacterial activity against fungi by filter paper method,and for the inhibition activity on cancer cells(HeLa cells) by CCK-8.Results The total protein concentrations of Nicotiana alata flowers,WT and transgenic K326 tobacco leaves were 11.25,10.33 and 10.14 mg/mL,respectively.The antibacterial activity of total protein from transgenic K326 tobacco leaves against Candida albicans was(85.68±3.08)%,which was 1.33 and 1.14 times that of total protein from WT K326 tobacco leaves and Nicotiana alata flowers,respectively(F=15 339,P <0.05);The antibacterial activity against Fusarium oxysporum was(148.48±2.47)%,which was 1.09 and 1.08 times that of total protein from WT K326tobacco leaves and Nicotiana alata flowers,respectively(F=4.927,P <0.05).The IC_(50) value of transgenic K326 tobacco leaf protein on HeLa cells was the smallest(6.11 mg/mL),and the inhibitory activity was 1.56 and 1.21 times that of total protein of WT K326 tobacco leaves and Nicotiana alata flowers,respectively(F=89.748,P <0.05).Conclusion The total protein of K326 tobacco with high expression of NaD1 gene has good antibacterial and anticancer bioactivities,which provides an experimental basis for producing antibacterial and anticancer biological agents with tobacco as bioreactor.

11.
J. res. dent ; 10(4): 12-17, out.-dez.2022.
Article de Anglais | LILACS-Express | LILACS | ID: biblio-1411529

RÉSUMÉ

Introduction: Bioceramic sealers have been gaining prominence in endodontics, meaning a great advance for endodontic therapy, mainly due to their biocompatibility and bioactivity. Objectives: research and discuss the literature about the bioactivity and biocompatibility of bioceramic sealants. Materials and methods: An integrative review was performed. The systematic plan consisted of four steps. In the first stage, a bibliographic survey was carried out in the Capes Periódicos Portal database. The search criteria were articles published between 2017 and 2022, found in the "advanced search" mode, using cross-references with the following keywords: bioceramics sealers AND endodontology AND root canals AND endodontic sealer AND bioactivity AND biocompatibility. Only documents found and published in full were evaluated. In the second stage, the titles and abstracts of the articles were read. In the third stage, a selection was made of those containing 3 to 5 keywords and a wording equal to or close to the proposed keywords. The fourth step consisted of reading the texts in full, followed by checking for duplicity and building a table with the collected information. Results: In view of the research carried out, 23 articles were obtained in the first stage. After reading the titles and abstracts, 21 articles were obtained. After selecting those containing 3 to 5 keywords, 10 articles met the criteria. One article was deleted due to duplicates. 9 articles met the exclusion and inclusion criteria and were selected to be part of the integrative review. Discussion: The selected studies in this literature review showed that bioceramic endodontic sealants perform well in endodontic therapy. Conclusion: To advance in its clinical application, more in vivo and in vitro studies with precise methods are needed to obtain more reliable data about its properties.

12.
Acta biol. colomb ; 27(3): 403-414, Sep.-Dec. 2022. tab, graf
Article de Anglais | LILACS-Express | LILACS | ID: biblio-1573582

RÉSUMÉ

ABSTRACT In the present study, the phytochemical composition, antioxidant and anti-hemolytic activities of root and aerial part Rubia tinctorum hydromethanolic extracts were investigated. Phytochemical screening reveals the presence of phenol, alkaloids, coumarin, flavonoids, and tannins in both extracts. LC-ESI-MS/MS analysis showed the presence of 15 and 17 components in the root and the aerial part extracts, respectively. The quantitative analysis indicates the richness of root extracts in phenolic compounds (118.38 mg GAE/g) and flavonoids (45.29±0.04 mg GAE/g), the aerial part extract has the highest levels in tannins (134.1±0.1 mg GAE/g). Besides, aerial part extracts revealed the highest antioxidant activity for DPPH (83.23 %) and FRAP (1.51±0.22), while the root extract exhibited the highest potential for TAC and antihemolytic activity (61.09 %). Rubia tinctorum contains different active compounds to prevent diseases related to oxidative stress.


RESUMEN En el presente estudio se investigó la composición fitoquímica y las actividades antioxidante, y antihemolítica de los extractos hidrometanólicos de la raíz y la parte aérea de R. tinctorum. El cribado fitoquímico revela la presencia de fenol, alcaloides, cumarina, flavonoides y taninos en ambos extractos. El análisis LC-ESI-MS/MS mostró la presencia de 15 y 17 componentes en los extractos de la raíz y la parte aérea, respectivamente. El análisis cuantitativo indica la riqueza de los extractos de la raíz en compuestos fenólicos (118, 38 mg GAE/g) y flavonoides (45, 29±0, 04 mg GAE/g), el extracto de la parte aérea tiene los niveles más altos en taninos (134, 1±0, 1 mg GAE/g). Además, los extractos de la parte aérea revelaron la mayor actividad antioxidante para DPPH (83, 23 %) y FRAP (1, 51 ±0, 22), mientras que el extracto de la raíz exhibió el mayor potencial para TAC y actividad antihemolítica (61,09 %). Rubia tinctorum contiene diferentes compuestos activos para prevenir enfermedades relacionadas con el estrés oxidativo.

13.
Bol. latinoam. Caribe plantas med. aromát ; 21(3): 343-351, mayo 2022. tab, ilus
Article de Anglais | LILACS | ID: biblio-1396913

RÉSUMÉ

Leaf and fruit decoctions of Schinus areira L. from northwest Argentina were investigated here. Phenolic compounds and organic acids were analyzed by HPLC. Antioxidant capacity and α-glucosidase inhibition were determined by using in vitro tests. The general toxicity was assessed against Artemia salina nauplii. Hyperoside and 3 O-caffeoylquinic acid in leaf decoctions; gallic acid and catechin in fruit decoction were the major phenolic compounds. Malic and citric acids were the main organic acid quantified in the leaf and fruit decoctions, respectively. Fruit decoction had a relatively important content of shikimic acid, precursor of Tamiflu. Leaf decoction presents a greater richness in bioactive compounds with antiradical activity against DPPH●, O2●-and ●NO radicals. S. areira leaves and fruits had α-glucosidase inhibitory activity comparable to hyperoside and acarbose. Fruit decoction was not eco-toxic; leaf decoction showed significant eco-toxic activity and could be chosen for the search of other bioactive compounds with pharmacological activity.


Se investigaron decocciones de hojas y frutos de Schinus areira L. del noroeste de Argentina. Compuestos fenólicos y ácidos orgánicos se analizaron mediante HPLC. Capacidad antioxidante e inhibición de α-glucosidasa se determinaron in vitro. Se evaluó toxicidad general con Artemia salina. Los principales compuestos fenólicos fueron hiperósido y ácido 3 O-cafeoilquínico en hojas y ácido gálico y catequina en frutos. Los principales ácidos orgánicos cuantificados fueron málico en hojas y cítrico en frutos. Ácido shikímico, precursor del Tamiflu está presente en decocción de frutos con un contenido relativamente importante. La de hojas presenta una mayor riqueza en compuestos bioactivos con actividad antirradicalaria frente a DPPH●, O2●-y ●NO. Las hojas y frutos de S. areira tenían una actividad inhibidora de la α-glucosidasa comparable a la de hiperósido y acarbosa. La decocción de frutas no fue eco-tóxica, pero sí la de hojas que podría ser fuente de compuestos bioactivos con actividad farmacológica.


Sujet(s)
Extraits de plantes/composition chimique , Anacardiaceae/composition chimique , Antioxydants/composition chimique , Extraits de plantes/toxicité , Chromatographie en phase liquide à haute performance/méthodes , Feuilles de plante/composition chimique , Acides Organiques/analyse , Composés Phénoliques , Inhibiteurs des glycoside hydrolases , Fruit/composition chimique
14.
European J Med Plants ; 2022 Apr; 33(4): 25-35
Article | IMSEAR | ID: sea-219479

RÉSUMÉ

Aims: Ocimum gratissimum is an aromatic and medicinal plant, well known for its medicinal values such as antifungal properties. This study aimed at evaluating the effect of T. diversifolia powder and compost use as biofertilizer on the growth parameters, essential oil (EO), total phenolic and flavonoid content and anticandida activity of O. gratissimum. Study Design: O. gratissimum plants were cultivated for 8 months in an experimental farm designed as a split plot into 4 blocks. Each block was amended (main factor) either with T. diversifolia compost (150 g/plant), powder (40 g/plant) or the synthetic fertilizer NPK (10 g/plant), respectively, followed by sprayed (second factor) with same fertilizer at 20 g/L, 20 g/L and 2 g/L or water every two weeks after transplantation. The control block received no amendment and was sprayed with water or the previous fertilizer. Place and Duration of Study: This work was carried out within August 2019 to October 2020 in Yaoundé-Cameroon. Methodology: Plant growth parameters (fresh and dry leaves weight, florescent weight and plant height) were evaluated at four and eight months after transplantation. The harvested fresh leaves were hydrodistillated for EO and the hot aqueous extract. Both extracts were used for the evaluation of the anticandida activity while the latter was submitted to total flavonoids and phenolic analyses. Results: At 4 and 8 months after transplantation, the synthetic fertilizer and T. diversifolia compost significantly increased plant growth parameters as compared to other treatments. The plants treated with T. diversifolia biofertilizer compost showed the highest total phenolic (53.16 µg GAE/µL), flavonoid (36.32 µg// GAE/µL) content, and EO yield (0.666%). The EO from O. gratissimum treated with T. diversifolia compost showed the best inhibitory activity on C. albicans NR-29451. Conclusion: This study showed that T. diversifolia compost was a promising organic fertilizer in optimizing the growth, secondary metabolites and anticandida activity of O. gratissimum.

15.
Arch. latinoam. nutr ; Arch. latinoam. nutr;72(1): 11-22, mar. 2022. tab, graf
Article de Espagnol | LILACS, LIVECS | ID: biblio-1368344

RÉSUMÉ

El arándano (Vaccinium corymbosum L.) posee un alto contenido de compuestos fenólicos los cuales han sido estudiados principalmente por su actividad antioxidante, antiobesogénica, antiinflamatoria, entre otras. Objetivo. Evaluar el efecto de la digestión gastrointestinal in vitro sobre la bioaccesibilidad de compuestos fenólicos y actividad antioxidante de una formulación nutracéutica de arándano (cápsula), comparado con arándano fresco y polvo. Materiales y métodos. Se obtuvieron extractos metanólicos de muestras de arándano fresco y liofilizado y se determinó su contenido de fenoles, flavonoides y antocianinas totales, así como también actividad antioxidante. Se llevó a cabo un ensayo de simulación de digestión gastrointestinal para evaluar la bioaccesibilidad de los compuestos fenólicos presentes en las muestras. Resultados. Los resultados mostraron que la digestión gástrica de arándano en polvo y en cápsula promovió una mayor bioaccesibilidad de fenoles (42% y 40%), flavonoides (52% y 33%) y antocianinas (45% y 40%) comparado con digestos de arándano fresco. Posterior a la digestión intestinal, la bioaccesibilidad de fenoles (63%) y flavonoides (67%) fue mayor en la cápsula de arándano comparada con su contraparte arándano en polvo. Las condiciones de digestión intestinal afectaron negativamente la bioaccesibilidad de las antocianinas independientemente del tipo de muestra evaluada. Conclusión. Las condiciones de digestión gástrica promueven una mayor estabilidad de los compuestos fenólicos en arándano en polvo y en cápsula lo que pudiera ser relevante para el mantenimiento de un ambiente antioxidante a este nivel. Las condiciones de digestión intestinal afectaron de manera particular a los compuestos fenólicos de arándano fresco y polvo, pero no a la cápsula, lo que puede sugerir que el encapsulamiento protegió de las condiciones alcalinas a los fenoles presentes. Se sugieren estudios posteriores sobre absorción in vitro de los componentes remanentes en intestino y sus posibles efectos sobre biomarcadores de estrés oxidativo en modelos in vivo(AU)


Blueberry (Vaccinium corymbosum L.) has a high content of phenolic compounds which have been studied mainly for their antioxidant, antiobesogenic, anti-inflammatory activity, among others. Objetive. The objective of the present study was to evaluate the effect of in vitro gastrointestinal digestion on the bioaccessibility of phenolic compounds and antioxidant activity of a nutraceutical formulation of blueberry (capsule), compared to fresh and powder blueberry. Materials and methods. Methanolic extracts of fresh and lyophilized blueberry were obtained and determined its total phenols, flavonoids, anthocyanins content, as well as antioxidant activity. A gastrointestinal digestion simulation test also was carried out to assess the bioaccessibility of the phenolic compounds found in samples. Results. The results showed that gastric digestion of powder and capsule blueberry promoted greater bioaccessibility of phenols (42% and 40%), flavonoids (52% and 33%) and anthocyanins (45% and 40%), compared to fresh blueberry digests. After intestinal digestion, the bioaccessibility of phenols (63%) and flavonoids (67%) was higher in the blueberry capsule compared to its powdered blueberry counterpart. The intestinal digestion conditions negatively affected the bioaccessibility of anthocyanins regardless of the type of sample evaluated. Conclusion. Gastric digestion conditions promote greater stability of phenolic compounds in powdered and capsule blueberries, which could be relevant for the maintenance of an antioxidant environment at this level. The intestinal digestion conditions particularly affected the phenolic compounds of fresh and lyophilized blueberry, but not the capsule, which may suggest that encapsulation protected the phenols present from alkaline conditions. Further studies on in vitro absorption of the remaining components in the intestine and their possible effects on oxidative stress biomarkers in in vivo models are suggested(AU)


Sujet(s)
Tanins , Flavonoïdes , Myrtillier , Composés Phénoliques , Absorption gastro-intestinale , Techniques in vitro , Maladie chronique , Digestion , Lyophilisation
16.
Chinese Herbal Medicines ; (4): 90-96, 2022.
Article de Chinois | WPRIM | ID: wpr-953614

RÉSUMÉ

Objective: To investigate the immunomodulatory activity of polysaccharides from the roots of Brassica rapa. Methods: The crude polysaccharide from roots of B. rapa (BRP) was extracted and purified to further investigate the active fraction of BRT for inducing macrophage phagocytosis. Results: Effects on RAW264.7 cells demonstrated that BRP behaved better phagocytic capacity and had potent immunomodulatory activity, including increasing production of nitric oxide (NO), tumor necrosis factor α (TNFα) and upregulating mRNA levels of inducible NO synthase (iNOS) and TNFα. Furthermore, modulation of macrophage by BRP was indicated to be mediated via the activation of Akt and nuclear factor-kappa B (NF-κB). Conclusion: The beneficial effects of BRP could be used as an immunotherapeutic adjuvant in treatment of inflammatory diseases.

17.
Article de Chinois | WPRIM | ID: wpr-1014803

RÉSUMÉ

Isopentenyl flavonoids are a class of characteristic components in Sophora flavescens Ait. (S. flavescens). They have biological activities such as anti-tumor, anti-bacteria, anti-inflammol/ Lation and anti-oxidation. In this paper, the structural types, toxicology and pharmacological effects of isopentenyl flavonoids from S. flavescens were briefly reviewed. Furthermore, the worth of further study on pharmacokinetics, pharmacodynamics, toxicology, action targets, molecular mechanisms and structure-function relationships of isopentenyl flavonoids were proposed. The deep exploration on functional characterastics of isopentenyl flavonoids of S. flavescens and their application on development of innovative drugs are of great significance to further improve the added value of isopentenyl flavonoids and expand their application fields.

18.
Braz. dent. sci ; 25(3): 1-11, 2022. tab, ilus, graf
Article de Anglais | LILACS, BBO | ID: biblio-1373062

RÉSUMÉ

Objective: This study aimed to evaluate the radiopacity, compressive strength, setting time and bioactivity of Biodentine after modification with Ytterbium Tri-Fluoride (YbF3) in three different concentrations. Material and Methods: Radiopacity was determined using the equivalence in millimeters of aluminum (mm Al) from digital radiographs. Compressive strength was evaluated using a universal testing machine. The initial and final setting times were evaluated using Gillmore needle. The bioactive potential was evaluated using the environmental scanning electron microscope (ESEM) connected with Energy Dispersive X-ray analysis (EDX) and X-ray Diffractometer (XRD) at three different time intervals. pH was measured using a pH-meter. Data were analyzed using one-way ANOVA followed by Tukey's post hoc tests (P≤ 0.05). Results: Radiopacity of Biodentine with 2.5%, 5% and 7.5% YbF3 was significantly higher than unmodified Biodentine (P ≤ 0.05). Unmodified Biodentine showed the highest mean compressive strength values compared to all other groups (p≤ 0.05). The addition of YbF3 to Biodentine has extended the final setting time except for the 2.5% YbF3 group that showed no significant difference compared to the control. All groups showed an alkaline pH at 28 days, ESEM coupled with EDX analysis showed evidence of dense globules of calcium phosphate on the surface indicating enhancement of bioactivity. Conclusion: 2.5% YbF3 can be a promising radiopacifying agent to Biodentine with improvement in radiopacity, bioactive potential and maintaining the setting time and compressive strength at acceptable level as indicated by the ISO standards (AU)


Objetivo: O estudo buscou avaliar a radiopacidade, resistência à compressão, tempo de presa e bioatividade da Biodentina modificada por Trifluoreto de itérbio (YbF3). Materiais e Métodos: YbF3 foi incorporado ao pó de Biodentina e dividido em 4 grupos (X0, X1, X2 e X3) de acordo com a concentração de YbF3; 0%, 2.5%, 5% e 7.5% do peso, respectivamente. Os espécimes foram preparados para cada experimento com um total de 40 espécimes para cada teste (n=10 por grupo). A radiopacidade foi determinada através do uso equivalente em milímetros de alumínio. A resistência a compressão foi avaliada através do uso de uma máquina de teste universal. O tempo inicial e final foi avaliado através de uma agulha de Gillmore. O potencial bioativo foi avaliado através do Microscópio Eletrônico de Varredura (ESEM), conectado com a análise de espectroscopia de raios X por energia dispersiva (EDX) e difração de raio X (DRX). O pH foi mensurado através do uso de um pHmetro. Os dados foram analisados pelo ANOVA seguido do Test Tukey. Resultados: A radiopacidade nos grupos X1, X2 e X3 foi significantemente maior do que no grupo X0. O grupo X0 mostrou a maior resistência à compressão comparado com os outros grupos. A adição de YbF3 a Biodentina excedeu o tempo final exceto pelo grupo X1, que não apresentou diferença estatística significante quando comparado com o grupo X0. Todos os grupos apresentaram um pH alcalino com 28 dias. ESEM com análise EDX mostrou evidência de densos glóbulos de fosfato de cálcio na superfície, indicando uma melhora na bioatividade. Conclusão: 2.5% YbF3 é um promissor radiopacificador para Biodentina, que aumentou a radiopacidade e potencial bioativo enquanto manteve o tempo definido e a resistência à compressão em níveis aceitáveis como indica a norma ISO.(AU)


Sujet(s)
Ytterbium , Produits de contraste , Matériaux dentaires , Odontologie
19.
Biota Neotrop. (Online, Ed. ingl.) ; 22(spe): e20221356, 2022. graf
Article de Anglais | LILACS-Express | LILACS | ID: biblio-1394012

RÉSUMÉ

Abstract In Brazil, research with natural products had a strong impulse when FAPESP supported the creation of the Laboratory of Chemistry of Natural Products of the Institute of Chemistry of USP (1966). In 1999, FAPESP launched the Research Program in the Characterization, Conservation, Restoration and Sustainable Use of Biodiversity (BIOTA-FAPESP), which intensified the sustainable exploitation of biodiversity, and which evolved to form the Biota Network for Bioprospection and Bioassays (BIOprospecTA), which integrates groups from all over the country, optimizing the use of the skills already installed for the bioprospecting of microorganisms, plants, invertebrates, vertebrates and marine organisms. Of the 104 projects related to plant sciences, 35 carried out bioprospection of Brazilian flora, belonging to the areas of Chemistry, Botany, Genetics, Plant Physiology, Plant Morphology, Plant (Chemo)taxonomy, Ecosystem Ecology, Plant Genetics. Physical Sciences, Forest Resources, Forestry Engineering, Agronomy, leading to thousands of publications, engagement of hundreds of students and a deeper understanding of natural products in different biological models through macromolecules analysis aided by computational and spectrometric strategies, in addition to pharmacological evaluations. The development of omics approaches led to a more comprehensive view of the chemical profile of an organism, and enabled integrated and concomitant studies of several samples, and faster annotation of known molecules, through the use of hyphenated and chemometric techniques, and molecular networking. This also helped to overcome the lack of information on the safety and efficacy of herbal preparations, in projects dealing with the standardization of herbal products, according to international standards. The BIOTA-FAPESP program has also focused on environmental aspects, in accordance with the principles of Green Chemistry and has had positive effects on international collaboration, on the number and impact of scientific publications and on partnership with companies, a crucial step to add value and expand the production chain of bioproducts. Also, the compilation, systematization and sharing of data were contemplated with the creation of the NUBBEDB database, of free access, and that integrates with international databases (ACD/labs, American Chemical Society - ACS), helping researchers and companies in the development from different areas of science, technology, strengthening the bioeconomy and subsidizing public policies.


Resumo No Brasil, as pesquisas com produtos naturais tiveram um forte impulso quando a FAPESP apoiou a criação do Laboratório de Química de Produtos Naturais do Instituto de Química da USP (1966). Em 1999, a FAPESP lançou o Programa de Pesquisa em Caracterização, Conservação, Restauração e Uso Sustentável da Biodiversidade (BIOTA-FAPESP), que intensificou a exploração sustentável da biodiversidade, e que evoluiu para formar a Rede Biota de Bioprospecção e Bioensaios (BIOprospecTA), que integra grupos de todo o país, otimizando o aproveitamento das competências já instaladas para a bioprospecção de microrganismos, plantas, invertebrados, vertebrados e organismos marinhos. Dos 104 projetos relacionados às ciências vegetais, 35 realizaram a bioprospecção da flora brasileira, em diversas áreas como Química, Botânica, Fisiologia e Morfologia Vegetal, (Quimio)taxonomia Vegetal, Ecologia de Ecossistemas, Genética Vegetal, Recursos Florestais, Engenharia Florestal, dentre outros, levando a milhares de publicações, ao engajamento de centenas de estudantes e ao entendimento mais profundo dos produtos naturais em diferentes modelos biológicos por meio da análise de micromoléculas auxiliada por estratégias computacionais e espectrométricas, além de avaliações farmacológicas. O desenvolvimento de abordagens ômicas ampliou a visão sobre perfil químico dos organismos, possibilitou o estudo integrado e concomitante de várias amostras, e a anotação mais rápida de moléculas conhecidas, por meio do uso de técnicas hifenadas, quimiométricas e redes moleculares. Isso também contribuiu para superar a falta de informação sobre a segurança e eficácia dos fitopreparados, em projetos que tratam da padronização de produtos fitoterápicos, de acordo com normas internacionais. O programa BIOTA-FAPESP também tem focado em aspectos ambientais, de acordo com os princípios da Química Verde e teve reflexos positivos na colaboração internacional, no número e no impacto das publicações científicas e na parceria com empresas, etapa crucial para agregar valor e expandir a cadeia produtiva de bioprodutos. Ainda, a compilação, sistematização e compartilhamento de dados foram contemplados com a criação da base de dados NUBBEDB, de livre acesso, e que se integra com bases internacionais (ACD/labs, American Chemical Society - ACS), auxiliando pesquisadores e empresas no desenvolvimento de diferentes áreas da ciência, tecnologia, fortalecendo a bioeconomia e subsidiando políticas públicas.

20.
Braz. J. Pharm. Sci. (Online) ; 58: e19660, 2022. tab, graf
Article de Anglais | LILACS | ID: biblio-1394027

RÉSUMÉ

Abstract In an attempt to increase molecular stability and provide controlled release, vascular endothelial growth factor (VEGF) was encapsulated into polycaprolactone (PCL) nanoparticles. Both VEGF-free and VEGF-loaded PCL nanoparticles were formulated by w/o/w double emulsion of the dichloromethane-water system in the presence of polyvinyl alcohol (PVA) and rat serum albumin. To achieve the optimal formulation concerning particle size and monodispersity, studies were carried out with different formulation parameters, including PVA concentration, homogenization time and rate. Scanning electron microscopy and dynamic light scattering analysis showed respectively that particles had a spherical shape with a smooth surface and particle size varying between 58.68-751.9 nm. All of the formulations were negatively charged according to zeta potential analysis. In vitro release study was performed in pH 7.4 phosphate-buffered saline at 37°C and released VEGF amount was measured by enzyme-linked immunosorbent assay (ELISA) method. At the end of the 35th day, 10% of total encapsulated VEGF was released with a sustained-release profile, which fitted the Korsmeyer-Peppas kinetic model. The bioactivation of the nanoparticles was evaluated using XTT and ELISA methods. As a result, the released VEGF was biologically active and also VEGF loaded PCL nanoparticles enhanced proliferation of the human umbilical vein endothelial cells in cell culture.


Sujet(s)
Facteur de croissance endothéliale vasculaire de type A , Nanoparticules/classification , Techniques in vitro/méthodes , Test ELISA/méthodes , Microscopie électronique à balayage/méthodes , Techniques de culture cellulaire/méthodes , Cellules endothéliales de la veine ombilicale humaine
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