Insights and compliance challenges for nutraceutical regulations in Indian market

Nutraceuticals, comprising supplements, herbal medicines, vitamins, and minerals, claim therapeutic benefits in addressing disease causes. Coined in 1989, the term denotes compounds promoting health. Projections suggest India's nutraceutical business will reach USD 18 billion by 2025, necessitating robust oversight. Food Safety and Standards Authority of India, established under Food Safety and Standards Act of 2006, governs approvals. This study aimed to collate data on approved, refused, and withdrawn nutraceutical products and ingredients in India from 2020 to 2023. During this period, 58 out of 110 applications, constituting 52.7%, gained approval. Notably, 20-40% of applications related to nutraceuticals faced refusal or withdrawal within the preceding four years. Factors contributing to rejections and withdrawals include the sorting of S-adenosyl-L-methionine (SAMe) as a pharmaceutical compound, non-compliance with recommended dietary intake, potential drug-like properties, misleading labeling, and insufficient evidence of product efficacy. Withdrawals often result from issues like adulteration, substandard ingredients, and noncompliance with Good Manufacturing Practices (GMP) regulations. To surmount these challenges, FSSAI must establish rigorous regulations and guidelines for nutraceutical promotion, including public notifications for any modifications, with a concurrent expectation for industries to adhere to these guidelines in promoting public health. The collaboration of applicants/nutraceutical industry, and FSSAI would foster stable expansion, as evident in the CAGR for nutraceutical products and ingredients in India.

Effects of different fortified feeding methods on the nutrient metabolism and growth rate of very low birth weight preterm infants / 中国儿童保健杂志

【Objective】 To analyze the effects of different fortified feeding methods on nutritional metabolism and growth rate of preterm very low birth weight infants (VLBWI), in order to provide new clues for improving the prognosis of the preterm infants. 【Methods】 A total of 115 cases of premature VLBWI admitted to Department of Neonatology, The First Affiliated Hospital of Kunming Medical University from January 2019 to December 2020 were included in this study, and were divided into fortified breastfeeding group (HFM group), mixed feeding group, and premature formula feeding group (PF group) based on their feeding methods. The effects of different feeding methods on the nutritional metabolism and growth rate of premature VLBWI were analyzed. 【Results】 1) The hospitalization time of infants in the HFM group was shorter than that in PF group and mixed feeding group (t=7.185, 6.924, P<0.05). 2) The proportion of necrotizing enterocolitis (NEC) in the HFM group during hospitalization was lower than that in the PF group (P<0.05); the proportions of late onset septicemia(LOS) and extra uterine growth restriction(EUGR) in the HFM group during hospitalization were lower than those in the PF group (χ2=5.030, 4.147, P<0.05); the proportion of LOS was lower than that of the mixed feeding group(χ2=6.589, P<0.05). 3) During hospitalization, the proportions of abdominal distension, bloody stools and increased eosinophils in the HFM group were lower than those in the PF group (P<0.05), which in mixed feeding group was lower than those in PF group (Fisher exact test, P<0.05). 4) At discharge, the weight and length growth rate of the HFM group were higher than those of the mixed feeding group (t=3.722, 0.425, P<0.001) and the PF group (t =6.015, 0.496, P< 0.001). 【Conclusion】 Fortified breastfeeding can more effectively increase the growth rate of VLBWI in premature infants, improve nutritional metabolism, reduce complications and adverse feeding reactions related to premature infants, and is safer and more effective.

Effect of Hormones on Rooting of Apical Cuttings for Propagation of Guava Cv. Lucknow-49

An attempt was made on the propagation of Guava cv. Lucknow-49 during the period of 2022-23 from apical cuttings under mist chamber conditions was undertaken. The apical cuttings were collected from shoot tips measuring about 10 to15 cm treated with different hormones of concentrations viz., IAA @ 2000, 3000 and 4000 ppm; NAA @ 2000, 3000 and 4000 ppm along with IBA @ 3000, 4000 and 5000 ppm and these were compared with control, the treated apical cuttings were planted in 40 cavity protrays and kept till 30 days for root induction under mist conditions. The different growth hormonal applications and its varied concentrations excerted a significant effect on root development in cuttings. Apical cuttings treated with IBA @ 5000 ppm was found better for rooting percentage (69.90 %), survival percentage (67.50 %), time taken to root (23.75 days), number of roots per cutting (15.15, 16.25, 17.53 and 24.64) and root length (7.33, 9.78, 13.85 and 18.33 cm) respectively at 30, 60, 90 and 120 DAP, fresh root weight (3.93 and 10.15 g), dry root weight (1.13 and 2.18 g) at 60 and 120 DAP, respectively. The rooting hormone IBA performed better in formation of rooting in apical cuttings when compared to IAA, NAA and untreated control. It can be revealed from the current study that propagation of guava through apical cuttings when treated with IBA @ 5000 ppm was proved as a better propagation method for rooting of cuttings.

The Trends and Growth Analysis in the Area, Production and Productivity of Turmeric in Surguja District of Chhattisgarh, India

Turmeric is an important spice crop grown in Chhattisgarh. The present study is mainly based on time series data. The secondary data on area, production and productivity of Turmeric in Chhattisgarh, were collected for the period 2001-02 to 2020-21from various publications. To analyze the trends and growth rate in the area, production and productivity of turmeric in Surguja district of Chhattisgarh state. The performance of turmeric was examined by estimating the growth rates and instability index of area, production and productivity of turmeric. Growth analysis for area, production and productivity. Focus on the Surguja district. It was positive and non-significant in the area of compound and linear growth rate, but it was positive and significant in the case of production. In terms of productivity, both compound and linear growth rate showed negatively significance. In the case of Chhattisgarh, the linear and compound growth rates in the area and production of turmeric were observed as positive and non-significant. While the growth rate in productivity in Chhattisgarh was found negatively and significant over the period. The major of constraints faced by the selected area, unavailability of improved and high yielding varieties, package and practices and Weather fluctuation. Ensure to availability of Improved and high yielding varieties for good production, productivity and good quality and Weather based information should provide for turmeric grower region.

Crecimiento diamétrico de Guarea guidonia (L.) estimado con modelos no lineales. bosque seco tropical en el norte de Tolima. Colombia. / Estimate diametric growth of Guarea guidonia (L.), with non-linear matematics models. natural dray forest in Tolima north. Colombia.

Introducción: Con el cómputo de modelos matemáticos es posible estimar el tiempo que toma una especie en conseguir un crecimiento máximo o asintótico. En este cálculo, el diámetro de un árbol, a la altura del pecho (D.A.P.), es una variable cómoda de medir durante largo tiempo. La diferencia entre cierto periodo de tiempo determinará incrementos que luego serán utilizados para el cálculo del crecimiento. El proceso se enriquece cuando se miden varios árboles de diferentes clases diamétricas. Objetivo. Calcular el crecimiento asintótico de Guarea guidonia (L.), con base en el Incremento Corriente Anual (ICA) de los D.A.P medidos en doce árboles, durante catorce años. Materiales y métodos. Los árboles se encuentran en una parcela permanente de investigación dentro de la reserva "Vallecita" en el municipio de Alvarado, al norte del departamento de Tolima, Colombia. Los modelos matemáticos no lineales utilizados fueron los de Von Bertalanffy, Gompertz y Logístico. Los perfiles obtenidos con las ecuaciones fueron comparados por medio del estadístico R2 de regresiones lineales. Resultados: Se obtuvo que G. guidonia presenta un ICA máximo de 0.58 cm/año y el promedio general fue de 0.51 cm/año. Por los modelos de Von Bertalanffy y Gompertz, se estima que, desde un diámetro base de 10 cm, G. guidonia tardaría 89 años en llegar a un diámetro de 50 cm., y que, con el modelo Logístico, alcanzaría el mismo diámetro en 83 años. Al comparar los tres perfiles del crecimiento diamétrico acumulado de la especie se encontró que entre ellos no se presentan diferencias estadísticas significativas. En general, se tiene que, comparado con otras especies como Anacardium excelsum (Kunt), la G. guidonia presenta un crecimiento diamétrico lento. Conclusión. G. guidonia es una especie que requiere de planes de manejo que propendan por la conservación y el óptimo desarrollo de esta especie.

Introduction: Through the calculation of mathematical models, it is possible to estimate the time it takes for a species to reach maximum or asymptotic growth. In this calculation, the diameter of a tree, at breast height (DBH), is a convenient variable to measure over a long period of time. The difference between a given period of time will determine the increments that will later be used to calculate growth. The process is enriched when several trees of different diameter classes are measured. Objective. To calculate the asymptotic growth of Guarea guidonia (L.), from the Annual Running Annual Growth (ACA) of the DBH, measured in twelve trees, during fourteen years. Materials and methods. The trees are located in a permanent research plot within the "Vallecita" reserve in the municipality of Alvarado, in the north of the department of Tolima, Colombia. The nonlinear mathematical models used were those of Von Bertalanffy, Gompertz and Logistic. The profiles obtained with the equations were compared using the R2 statistic of linear regressions. Results: G. guidonia had a maximum ACA of 0.58 cm/year and the average was 0.51 cm/year. Using the Von Bertalanffy and Gompertz models, it is estimated that, from a base diameter of 10 cm, G. guidonia would take 89 years to reach a diameter of 50 cm, and that, with the Logistic model, it would reach the same diameter in 83 years. Comparing the three growth profiles, it is estimated that, from a base diameter of 10 cm, G. guidonia would take 89 years to reach a diameter of 50 cm. and that, with the logistic model, it would reach the same diameter in 83 years. When comparing the three cumulative growth profiles of the diameter of the species, it was observed that there were no significant statistics. In general, compared to other species such as Anacardium excelsum (Kunth), G. guidonia has a slow diametric growth. Conclusion. G. guidonia is a species that requires management plans for the conservation and optimal development of this species.

Trend Analysis of Area, Production and Productivity of Tomato in Uttar Pradesh, India

India has access to several natural resources because of its diverse Agro-climatic conditions and a wide-ranging and large raw material base suitable for vegetable cultivation. This study was purely based on secondary data as collected from reports from Horticulture at a Glance, Agriculture at a glance and the Indiastat site. The trends of area, production, and productivity were estimated from the period 2001–2002 to 2021–2022, under tomato cultivation. The Study period was further divided into three subperiods: period I (2001–2011), period II (2011–2022), and the overall period (2001–2022). The compound growth rates of area, production, and productivity computed at the national level was 2.93 percent, 4.89 percent, and 1.90 percent, respectively and in Uttar Pradesh it was found 3.81, 13.22 and 9.11 percent. This study showed that the percentage share of Uttar Pradesh under Tomato cultivation was higher than in the whole of India.

Studies on Dynamics of Growth Pattern in Chickpea and Their Correlations to Seed Yield under Different Sowing Dates in North– West India

The experiment was conducted with ten genotypes of chickpea (Cicer arietinum L.) viz ICCV 88503, ICCV 92944, HC- 1, HC-3, HC-5, H12-64, H13-01, H13-02, H14-01 and H14-04 for three dates of sowing i.e 15th October, 15thNovember and 15thDecember at the field in randomized block design during Rabi season of 2017-18 and 2018-19 at Pulses Section, Chaudhary Charan Singh Haryana Agricultural University, Hisar to observe the effect of sowing dates on vegetative and reproductive growth rate of chickpea genotypes at different intervals i.e 30, 60, 90, and 120 DAS with their correlations to seed yield. The dry weight of leaves, stem, pods, plant height, crop growth rate, relative growth rate and pollen viability were minimum in 15thDecember sowing and maximum in 15thOctober sowing at all intervals. Among genotypes, maximum dry matter and growth rate were observed in H12-64 and H13-01 while minimum were found in H14-04. Seed yield exhibited significant positive correlation with all traits in 15th October and 15th November sowing while non-significant was on 15th December sowing.

Evaluation of the Possibility of Establishing Certain Organic Cultivation Packages as Nutrient Sources for Chamomile (Matricaria chamomilla L.) Cultivation at Temperate Region of Uttarakhand, India

An utmost attempt was made to investigate the response of Chamomile (Matricaria chamomilla L.) to certain types and doses of organic manures at under open condition at temperate regions of Uttarakhand during the year 2019-2020. The study comprised of varying levels of three different organic manure sources namely, FYM, compost and vermicompost, along with a check treatment replicating thrice having plot having dimensions 1 m× 1 m with a spacing of 20 cm × 20 cm. The experiment was laid out in a randomized complete block design (RCBD). Forty-five (45) days old chamomile seedlings (attained about 10-15 cm in height) were transplanted during October, 2019 under open field condition. The soils of the experimental site were tested favourable with respect to some of the basic physical and chemical properties. The results revealed that maximum plant height (48.26 cm), number of branches per plant (35.40) and plant spread (33.09 cm) were significantly higher under T4 (FYM @ 25 t/ha) application at 180 days after transplanting. Application of T10 (VC @ 4 t/ha) produced early flowering (119.73 days). At bud initiation stage the highest total fresh (33.12 q/ha) and dry (7.59 q/ha) biomass yield were found in T4 (FYM @ 25 t/ha). At flowering stage maximum value of total fresh (60.02 q/ha) and dry (12.70 q/ha) biomass yield were found in T10 (VC @ 4 t/ha). The results, further revealed that T10 provided the fastest crop growth rate (0.86 g/m2/day). The highest number of flowers per plant (139.73), fresh flower yield (67.35 q/ha), dry flower yield (13.95 q/ha) and maximum cost: benefit (1:3.56) were obtained in plots which received FYM @ 25 t/ha. All the organic input treatments performed better over control and the treatment FYM @ 25 t/ha shall be recommended for profitable organic cultivation of chamomile.

Spatio-temporal variation in the growth of coral fragments of opportunity in the Eastern Tropical Pacific: implications for coral reef restoration

Introduction: Coral-reef communities are considered one of the most biodiverse, but also most threatened, marine ecosystems, and the accelerating loss of habitat over the past decades warrants active intervention. Objective: The present study demonstrates the successful implementation of a low-impact restoration technique in three Central Mexican Pacific degraded coral communities, using a protocol based on natural fragmentation (''fragments of opportunity") of the branching coral Pocillopora spp., considered the most abundant and primary carbonate-producing coral species of the Eastern Tropical Pacific. Methods: The restoration program was implemented in two offshore and one inshore coraline areas. The relationships between seawater temperature and coral survival, growth, and attachment rate were assessed over one year, with 183 fragments monitored each month. Results: The mean coral growth rate was 3.3 ± 0.1 mm mo-1, with annual growth rates in length and width of 39.9 ± 14.2 and 36.5 ± 19.5 mm yr-1, respectively. Self-attachment efficiency was 78 % and the survival rate was high (84 %). The growth rate differed significantly among reefs. Conclusions: Upon monitoring directly fragmented corals over a year, growth rates were deemed high enough to merit active restoration in the region. However, our data show that structural and abiotic differences and seasonal variability must be considered overall in successful long-term coral community restoration initiatives in the eastern Pacific region.

Introducción: Las comunidades de arrecifes de coral se consideran uno de los ecosistemas marinos con mayor biodiversidad, pero también los más amenazados, y la pérdida acelerada de hábitat en las últimas décadas justifica la implementación de una intervención activa. Objetivo: El presente estudio demuestra la implementación exitosa de una técnica de restauración de bajo impacto basada en la fragmentación natural (''fragmentos de oportunidad") del coral ramificado Pocillopora spp., la cual es la especie coralina más abundante y principal productora de carbonato del Pacífico Oriental Tropical. Métodos: El programa de restauración se implementó en dos sitios lejos de la costa y un sitio cercano a la costa, con comunidades coralinas degradadas. Las relaciones entre la temperatura del agua de mar y la supervivencia, el crecimiento y la tasa de adhesión de los corales se evaluaron durante un año con 183 fragmentos monitoreados cada mes. Resultados: La tasa media de crecimiento coralino fue de 3.3 ± 0.1 mm mo-1, con tasas de crecimiento anual en largo y ancho de 39.9 ± 14.2 y 36.5 ± 19.5 mm año-1, respectivamente. La eficiencia de la auto-adherencia fue del 78 % y la tasa de supervivencia fue alta (84 %). La tasa de crecimiento difirió significativamente entre los arrecifes. Conclusiones: Al monitorear directamente los corales fragmentados durante un año, las tasas de crecimiento se consideraron lo suficientemente altas como para merecer una restauración activa en la región. Sin embargo, nuestros datos muestran que las diferencias estructurales y abióticas y la variabilidad estacional deben considerarse en general en las iniciativas exitosas de restauración de comunidades de coral a largo plazo en la región del Pacífico oriental.

Nursery-reared coral outplanting success in an upwelling-influenced area in Costa Rica

Introduction: Environmental and intrinsic factors such as seawater temperature, salinity, nutrient concentration, upwelling, species, and life history can influence coral outplant survival and growth, and in consequence, the effectiveness of restoration. Thus, it is key to understand how these factors can shape coral outplant performance to ensure the long-term success of a restoration program. Objective: To establish the survival and growth rate of outplanted coral nursery-reared colonies of branching Pocillopora spp. and massive corals Pavona gigantea, Pavona clavus, and Porites lobata in Bahía Culebra, North Pacific of Costa Rica, and to determine whether the site of origin of the coral fragment and the presence of seasonal upwelling affected the growth of Pocillopora outplants. Methods: From September 2020 to September 2021, we monitored the survival, health, and growth of 30 Pocillopora spp. colonies from six donor sites, and 31 fragments of massive species (P. gigantea [n = 18], P. clavus [n = 8], P. lobata [n = 5]) that were outplanted to a degraded reef. We recorded in situ seawater temperature, salinity, and nutrient concentration. Results: By the end of the year, 100 % of the Pocillopora spp. outplants survived. Survival was 71.4 % for P. clavus, 47.5 % for P. gigantea, and 20 % for P. lobata. Coral tissue loss and predation marks were observed at least once in 71 % of massive outplants. Pocillopora spp. colonies increased their initial area in 333.1 %, at a rate of 9.98 ± 1.69 cm yr-1. The site of origin of the outplant influenced growth rate, but not the presence of seasonal upwelling. Massive species fragments grew at a rate of 1.35 ± 0.24 cm yr-1 for P. clavus, 1.48 ± 0.21 cm yr-1 for P. gigantea, and 0.61 cm yr-1 for P. lobata, with no differences among them. Conclusions: Environmental conditions at site level allow for high survival and growth of Pocillopora spp. outplants, previously considered as sensitive to stressors, and point towards acclimation to seasonal upwelling. Although survival of massive species was lower, developing a multi-species approach is key to enhance restoration success.

Introducción: Factores ambientales e intrínsecos como la temperatura del agua, salinidad, concentración de nutrientes, afloramiento, especies e historia de vida pueden influir en la supervivencia y crecimiento de los trasplantes de coral y, en consecuencia, en la eficacia de la restauración. Por ello, entender cómo estos factores pueden moldear el desempeño de los trasplantes es clave para asegurar el éxito de un programa de restauración a largo plazo. Objetivo: Establecer la supervivencia y tasa de crecimiento de los trasplantes de colonias de coral previamente cultivadas en viveros del coral ramificado Pocillopora spp. y las especies de crecimiento masivo Pavona gigantea, Pavona clavus y Porites lobata en Bahía Culebra, Pacífico Norte de Costa Rica, y determinar si el sitio donante de origen del fragmento de coral y la presencia de afloramiento estacional afectó el crecimiento de los trasplantes de Pocillopora. Métodos: De setiembre 2020 a setiembre 2021, se monitoreó la supervivencia, salud y crecimiento de 30 colonias de Pocillopora spp. de seis sitios donantes diferentes y 31 fragmentos de especies masivas (P. gigantea [n = 18], P. clavus [n = 8], P. lobata [n = 5]) que se trasplantaron a un arrecife degradado. Se registró la temperatura del agua, la salinidad y concentración de nutrientes in situ. Resultados: Al final del año, el 100 % de los trasplantes de Pocillopora spp. sobrevivieron. La supervivencia fue de 71.4 % para P. clavus, 47.5 % para P. gigantea y 20 % para P. lobata. Se observaron pérdidas de tejido y marcas de depredación al menos una vez en un 71 % de los trasplantes masivos. Las colonias de Pocillopora spp. aumentaron su área inicial en un 333.1 %, a una tasa de 9.98 ± 1.69 cm año-1. El sitio de origen del trasplante tuvo efecto sobre la tasa de crecimiento, pero no la presencia de afloramiento estacional. Los fragmentos de especies masivas crecieron a tasas de 1.35 ± 0.24 cm año-1 (P. clavus), 1.48 ± cm año-1 (P. gigantea) y 0.61 cm año (P. lobata), sin diferencias entre ellas. Conclusiones: Las condiciones del sitio permiten una alta supervivencia y crecimiento de trasplantes de Pocillopora spp., que previamente habían sido considerados sensibles a factores estresantes, e indican aclimatación a las condiciones locales de afloramiento estacional. Pese a que la supervivencia de las especies masivas fue menor, es esencial desarrollar un enfoque multi-especie para aumentar el éxito de la restauración.

Effect of BBM gene on callus growth and ginsenoside content in Panax quinquefolius / 中国中药杂志

Baby Boom(BBM) gene is a key regulatory factor in embryonic development and regeneration, cell proliferation, callus growth, and differentiation promotion. Since the genetic transformation system of Panax quinquefolius is unstable with low efficiency and long period, this study attempted to transfer BBM gene of Zea mays to P. quinquefolius callus by gene gunship to investigate its effect on the callus growth and ginsenoside content, laying a foundation for establishing efficient genetic transformation system of P. quinquefolius. Four transgenic callus of P. quinquefolius with different transformation events were obtained by screening for glufosinate ammonium resistance and molecular identification by PCR. The growth state and growth rate of wild-type and transgenic callus were compared in the same growth period. The content of ginsenoside in transgenic callus was determined by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS). The results showed that transgenic callus growth rate was significantly higher than that of wild-type callus. In addition, the content of ginsenoside Rb_1, Rg_1, Ro, and Re was significantly higher than that in wild-type callus. The paper preliminarily proved the function of BBM gene in promoting growth rate and increasing ginsenoside content, which provided a scientific basis to establish a stable and efficient genetic transformation system for Panax plants in the future.

A preoperative prediction model for breast benign and malignant phyllodes tumors / 肿瘤

Objective:To establish a predictive model for preoperative diagnosis of benign and malignant phyllodes tumor of the breast(PTB). Methods:The clinicopathological data of 69 patients with benign PTB and 41 patients with malignant PTB(24 borderline and 17 malignant)who underwent multiple(≥2)preoperative ultrasound follow-ups in the Cancer Hospital of Harbin Medical University from January 2011 to December 2018 were retrospectively analyzed.The preoperative prediction models of benign and malignant PTB were constructed by using the influencing factors determined by multivariate logistic regression analysis.The receiver operating characteristic(ROC)curve was used to evaluate the efficiency of the prediction model.In addition,the clinicopathological data of 22 patients of benign PTB and 19 patients of malignant PTB(12 borderline and 7 malignant)admitted to the hospital from January 2019 to April 2022 were selected for external verification. Results:Logistic regression analysis showed that growth rate of tumor>2 mm/month and ultrasound BI-RADS category≥4b were independent predictors for the diagnosis of malignant PTB(OR:4.476,95％CI:1.673～11.975;OR:9.448,95％CI:3.149～28.345;P<0.01).The logistic regression equation:Logit(P)=-1.868+1.499×growth rate of tumor+2.246×ultrasound BI-RADS category.The AUC for the training cohort was 0.795(95％CI:0.699～0.890),the best cut-off value was 0.421,the corresponding sensitivity was 0.732,the specificity was 0.826,and the Jorden index was 0.558,P<0.001.The AUC for the the validation cohort was 0.772(95％CI:0.624～0.919),with the sensitivity of 0.526 and the specificity of 0.773,positive predictive value was 0.667 and negative predictive value was 0.654,P = 0.003.The AUC of the training cohort and the validation cohort were both>0.75,indicating that the model has certain predictive ability. Conclusion:The predictive model constructed by clinicopathological parameters can be used for preoperative diagnosis of benign PTB and malignant PTB,and provide a certain reference value for clinicians to select the appropriate surgical resection scope.

Development trend of physical examination volume and revenue in health-checkup institutions in China from 2010 to 2019 / 中华健康管理学杂志

Objective:To investigate the development trend of physical examination volume and revenue in health-checkup institutions in China from 2010 to 2019.Methods:In this longitudinal study, the annual income, annual physical examination volume and other indicators reflecting institutional size were collected with questionnaire from 374 health-checkup institutions in the year of 2010, 2015 and 2019. The geometric mean method is used to calculate the compound annual growth rate (CAGR) of the annual physical examination volume, annual income, mean per capita cost from 2010 to 2015 and 2015 to 2019.Results:The annual physical examination volume of health-checkup institutions increased from 1.81 (1.00, 3.20) ×10 4 person times in 2010 to 5.08(3.50, 7.28)×10 4 person times in 2019; the CAGR of the physical examination volume from 2010 to 2015 was 14.04%(8.14%, 23.78%), and it was 9.49%(3.39%, 19.07%) from 2015 to 2019. The annual revenue increased from 768.73 (350.00, 1 623.75) ×10 4 yuan in 2010 to 3 500.00 (1 997.73, 6 818.54) ×10 4 yuan in 2019; the CAGR of annual revenue from 2010 to 2015 was 25.75% (15.17%, 35.09%), and it was 15.67% (8.78%, 26.11%) from 2015 to 2019. The mean per capita cost increased from 434.26 (278.82, 666.66) yuan in 2010 to 755.80 (506.90, 1 005.42) yuan in 2019; the CAGR of the mean per capita cost was 9.82% (1.71%, 17.10%) from 2010 to 2015, and it was 5.37% (0.95%, 10.46%) from 2015 to 2019. Conclusion:From 2010 to 2019, health-checkup institutions in China developed rapidly, and the CAGR of the annual physical examination volume, annual revenue, mean per capita cost are high.

Effect of iron-binding polypeptides and non-starch polysaccharides supplementation on growth performance and fecal scores of weaning pigs / Efecto de la suplementación con polipéptidos ligantes de hierro y polisacáridos no amiláceos sobre el crecimiento y diarrea en cerdos al destete / Efeito da suplementação com polipeptídeos de ligação de ferro e polissacarídeos não amiláceos no crescimento e diarreia em porcos ao desmame

Abstract Background: Weaning is the most stressful event in pig's life, resulting in postweaning diarrhea and growth retardation. The supplementation of Advanced Digestion Enhancing Protein Plus Technology (ADEPPTTM), which contains iron-binding polypeptides and non-starch polysaccharides, to nursery diets may reduce the occurrence of diarrhea and enhance growth performance of weaning pigs. Objective: To evaluate the effect of ADEPPTTM supplementation on growth performance and fecal score in weaning pigs. Methods: At weaning, a total of 54 weaning pigs (initial body weight: 7.42 ± 0.52 kg) were assigned to 3 treatments in 3 replicates with 6 pigs per pen based on sex, breed, and body weight for a 28-d feeding trial. The pigs were fed corn- soybean meal-based diets containing 0.0, 0.5, and 1.0% of the ADEPPTTM product in 2 phases (d 0-14 and d 15-28 postweaning, respectively). In the first week (d 3-5 postweaning) of the trial, a pig was removed from each pen when diarrhea was observed, housed in a separate pen within treatment, and then treated for 3 days with a 100-ml solution of electrolytes and ADEPPTTM by drenching. Growth performance and fecal score (1=normal to 4=watery diarrhea) were measured. Results: In the feeding trial, there were no significant differences in body weight, average daily gain, and average daily feed intake throughout the overall period. However, a quadratic trend was observed in gain to feed ratio (p=0.09) for d 0-7 postweaning and overall period with increasing ADEPPTTM supplementation levels in which the greatest value was observed in the 0.5% ADEPPTTM level. The fecal score tended to decrease linearly with increasing ADEPPTTM levels during d 0-7 (p=0.11) and 0-14 (p=0.12) postweaning. There was no significant difference on fecal score of diarrheic pigs and average daily gain tended to increase linearly in d 21-28 postweaning (p=0.08) with increasing ADEPPTTM levels. Conclusion: These results indicate that 0.5% ADEPPTTM supplementation has a potential to enhance growth performance of weaning pigs and might be effective to prevent and control postweaning diarrhea.

Resumen Antecedentes: El destete es el evento más estresante en la vida de los cerdos, resultando en diarrea posdestete y retraso en el crecimiento. La suplementación de dietas de destete con el producto Advanced Digestion Enhancing Protein Plus Technology (ADEPPTTM), el cual contiene polipéptidos ligantes de hierro y polisacáridos no amiláceos, puede reducir la aparición de diarrea y mejorar el crecimiento de los lechones destetados. Objetivo: Evaluar el efecto de la suplementación con ADEPPTTM sobre el crecimiento y la puntuación fecal en lechones destetados. Métodos: Al destete, se asignó un total de 54 cerdos (peso corporal inicial: 7,42 ± 0,52 kg) a 3 tratamientos, en 3 repeticiones, con 6 cerdos por corral, según sexo, raza y peso corporal para una prueba de alimentación de 28 días. Los cerdos se alimentaron con dietas a base de harina de maíz y soja que contenían 0,0, 0,5 y 1,0% de ADEPPTTM en 2 fases (d 0-14 y d 15-28 post-destete, respectivamente). En la primera semana del ensayo (d 3-5 después del destete) se extrajo de cada corral un cerdo con diarrea, se colocó en corral separado y luego se trató con una solución de 100 ml de electrolitos y ADEPPTTM, vía oral, durante 3 días. Se evaluaron el rendimiento del crecimiento y la puntuación fecal (1=normal a 4=diarrea acuosa). Resultados: En la prueba de alimentación no hubo diferencia significativa en el peso corporal, ganancia diaria o ingesta diaria de alimento. Sin embargo, se observó una tendencia cuadrática en la relación ganancia de peso: consumo de alimento (p=0,09) para los días 0-7 post-destete y el total del periodo con niveles crecientes de suplementación con ADEPPTTM, observándose el mayor valor en el nivel 0,5% de ADEPPTTM. La puntuación fecal tendió a disminuir linealmente con la suplementación incremental de ADEPPTTM durante los días 0-7 (p=0,11) y 0-14 (p=0,12) post- destete. No hubo diferencias significativas en la puntuación fecal de los cerdos con diarrea, mientras que la ganancia diaria tendió a aumentar linealmente en los días 21-28 post-destete (p=0,08) al aumentar los niveles de ADEPPTTM. Conclusión: Estos resultados indican que la suplementación con ADEPPTTM al 0,5% podría mejorar el rendimiento de crecimiento de los lechones destetados y ser eficaz para reducir la incidencia de diarrea post-destete.

Resumo Antecedentes: O desmame é o evento mais estressante na vida dos suínos, resultando em diarreia pós-desmame e retardo de crescimento. Suplementar as dietas de desmame com Advanced Digestion Enhancing Protein Plus Technology (ADEPPTTM), que contém polipeptídeos ligantes de ferro e polissacarídeos não amiláceos, pode reduzir a ocorrência de diarreia e melhorar o crescimento de leitões desmamados. Objetivo: Avaliar o efeito da suplementação de ADEPPTTM sobre o crescimento e o índice fecal de leitões desmamados. Métodos: Ao desmame, um total de 54 porcos (peso corporal inicial: 7,42 ± 0,52 kg) foram alocados em 3 tratamentos, em 3 repetições, com 6 porcos por curral, de acordo com sexo, raça e peso corporal para um teste de 28 dias alimentando. Os porcos foram alimentados com dietas à base de milho e farelo de soja contendo 0,0, 0,5 e 1,0% de ADEPPTTM em 2 fases (d 0-14 e d 15-28 pós-desmame, respectivamente). Na primeira semana do experimento (3-5 dias após o desmame), um porco com diarreia foi retirado de cada baia, colocado em baia separada e então tratado com uma solução de 100 ml de eletrólitos e ADEPPTTM, por via oral, por 3 dias. O desempenho do crescimento e a pontuação fecal (1=normal a 4=diarreia aquosa) foram avaliados. Resultados: No teste de alimentação não houve diferença significativa no peso corporal, ganho diário ou ingestão alimentar diária. No entanto, foi observada uma tendência quadrática no ganho de peso: relação do consumo de ração (p=0,09) para os dias 0-7 pós-desmame e o período total com níveis crescentes de suplementação com ADEPPTTM, com o maior valor sendo observado no nível de Suplementação de 0,5% com ADEPPTTM. O escore fecal tendeu a diminuir linearmente com a suplementação incremental de ADEPPTTM durante os dias 0-7 (p=0,11) e 0-14 (p=0,12) pós-desmame. Não houve diferenças significativas no escore fecal dos porcos com diarreia, enquanto o ganho diário tendeu a aumentar linearmente nos dias 21-28 pós-desmame (p=0,08) com níveis crescentes de suplementação com ADEPPTTM. Conclusão: Esses resultados indicam que a suplementação com 0,5% de ADEPPTTM pode melhorar o desempenho de crescimento de leitões desmamados e ser eficaz na redução da incidência de diarreia pós-desmame.

Effect of Canopy Gaps on Growth and Water Use Efficiency of Seedling of Vulnerable Species, Hopea sangal Korth

Aims: Hopea sangal Korth. is listed as vulnerable species and recently its remnant habitat was rediscovered in the degraded forest near the springs area in East Java. In the forest, the regeneration of the seedling and saplings understory is affected by the heterogeneity in environmental factors especially various light levels due to the canopy gaps. H. sangal is considered a shade-tolerant species, hence the establishment of the seedling in its natural habitat occurs under the closed canopy. This study aimed to understand the Water Use Efficiency (WUE) and Relative Growth Rate (RGR) of Hopea sangal Korth seedling grown in two different levels of tree canopy shades. Study Design: This research was using a completely randomized design, with 9 replications. Place and Duration of Study: This study was conducted in the Purwodadi Botanic Garden, East Java, between March � July. Methodology: We used seedling of H. sangal collected from the field in Pasuruan which were planted in plastic pots and acclimatized to obtain seedling of a similar size. The microclimate was measured weekly using solarimetri and sling psychrometer at 08.00; 10:00; 12:00; 14:00 and 16:00. RGR was measured by harvesting the seedling and whole plant WUE was measured using the gravimetric method. Results: We observed the light level during the periodic opening of the canopy gap was significantly different at a specific time (8-12 am), with the highest light intensity at 10.00 of 333.57 祄ol photon m-2s-1. The canopy shades differences did not affect the WUE of seedling (P = 0.333), meanwhile, there was a significant difference in the seedling RGR (P = 0.025). The seedling that were grown under a higher gap and received higher light intensity periodically during a day had higher RGR than those under a closed canopy. The WUE has a positive relationship with the RGR of seedling (R2 = 0.5; P < 0.05). Conclusions: This study suggested that the H. sangal is one of the shade-tolerant species capable of responding to gap-opening sunlight. The study also showed RGR of the seedling positively correlated with the WUE of plants, indicating that the seedling could use the water supply efficiently to grow rapidly.

Covid-19 Hazard Index: A Spatiotemporal Risk Forecast Tool

Background: In each geographic region, risk of new cases of COVID19 are driven by internal factors such as agent, host, and environment characteristics, as well as external factors, such as population mo-bility and cross border transmission of disease. COVID19 control measures are best implemented when local governments and health teams are well aware of these internal and external risks. These risks are dynamic in nature and hence need to be reviewed at regular intervals. The study conducted to develop a composite spatiotemporal Hazard Index comprising of three factors – presence of susceptible popula-tion, population density and presence of active cases with corresponding growth rates, to rank areas within an administrative boundary by their fortnightly risk of active COVID19 cases. Methods: Using Principal Component Analysis, the weights of each of these factors were determined and applied to transformed values of factors in the districts of Gujarat state for months of January to July 2021. Hazard Index thus obtained was used to rank the districts. Results: Spearman correlation between the Hazard Index and number of active cases 15 days later was moderate and significant (p<0.01) throughout the study period. Conclusion: Hazard Index can predict Districts at highest risk of active cases in the given time period. These districts with high Hazard Index would require different control measures, depending on the fac-tor that resulted in higher index value.

Effect of different protein based feed on the growth of mahseer / Efeito de alimentação baseada em proteínas diferentes no crescimento de mahseer

For the proper growth of fish, it is necessary to feed the fish with a proper and balanced diet. A study was conducted to find out the effect of different protein-based diets on fingerlings of Tor putitora (mahseer). A feed with dietary protein levels of 35%, 40%, 45%, and 50% were prepared. The effect of different protein-based feed on weight gain, standard growth rate (SGR), food conversion ratio (FCR), percent weight gain, food conversion efficiency (FCE), and protein efficiency ratio (PER) was studied. An increase was observed in the growth rate with an increase in protein concentration up to 45%. The fingerlings fed a 45% protein diet shown the highest growth, followed by 50%, 40%, and 35%. The SGR value was greatest for 45% protein diet (8.56) followed by 50% and 40%, while the least values were observed for 35% protein feed (1.57). The same trend was observed for FCE. The highest PER values was observed in fishes fed 45% protein-based feed (0.65) followed by 50% (0.56), 40% (0.38) and35% (0.17). The food conversion ratio was lowest for 45% protein diet (3.41), while the greatest for 35% protein feed (16.85). It was concluded that a 45% protein-based diet was the best feed formulation for higher production of Tor putitora. However, research on the same percentage of protein diet is recommended for yearlings.

Para o bom crescimento dos peixes, é necessário alimentá-los com uma alimentação adequada e balanceada. Um estudo foi realizado para descobrir o efeito de diferentes dietas à base de proteínas em alevinos de Tor putitora (mahseer). Foi preparado um alimento com níveis de proteína dietética de 35%, 40%, 45% e 50%. O efeito de diferentes alimentos à base de proteína no ganho de peso, taxa de crescimento padrão (SGR), taxa de conversão alimentar (FCR), ganho de peso percentual, eficiência de conversão alimentar (FCE) e taxa de eficiência proteica (PER) foi estudado. Foi observado um aumento na taxa de crescimento com um aumento na concentração de proteína de até 45%. Os alevinos alimentados com dieta de 45% de proteína apresentaram o maior crescimento, seguidos de 50%, 40% e 35%. O valor de SGR foi maior para dieta com 45% de proteína (8,56), seguido de 50% e 40%, enquanto os menores valores foram observados para ração com 35% de proteína (1,57). A mesma tendência foi observada para FCE. Os maiores valores de PER foram observados em peixes alimentados com 45% de ração à base de proteína (0,65), seguido por 50% (0,56), 40% (0,38) e 35% (0,17). A taxa de conversão alimentar foi menor para a dieta com 45% de proteína (3,41), enquanto a maior para a dieta com 35% de proteína (16,85). Concluiu-se que a dieta à base de proteína de 45% foi a melhor formulação alimentar para maior produção de Tor putitora. No entanto, a pesquisa sobre a mesma porcentagem de dieta proteica é recomendada para animais de um ano.

Effect of different protein based feed on the growth of mahseer

Abstract For the proper growth of fish, it is necessary to feed the fish with a proper and balanced diet. A study was conducted to find out the effect of different protein-based diets on fingerlings of Tor putitora (mahseer). A feed with dietary protein levels of 35%, 40%, 45%, and 50% were prepared. The effect of different protein-based feed on weight gain, standard growth rate (SGR), food conversion ratio (FCR), percent weight gain, food conversion efficiency (FCE), and protein efficiency ratio (PER) was studied. An increase was observed in the growth rate with an increase in protein concentration up to 45%. The fingerlings fed a 45% protein diet shown the highest growth, followed by 50%, 40%, and 35%. The SGR value was greatest for 45% protein diet (8.56) followed by 50% and 40%, while the least values were observed for 35% protein feed (1.57). The same trend was observed for FCE. The highest PER values was observed in fishes fed 45% protein-based feed (0.65) followed by 50% (0.56), 40% (0.38) and35% (0.17). The food conversion ratio was lowest for 45% protein diet (3.41), while the greatest for 35% protein feed (16.85). It was concluded that a 45% protein-based diet was the best feed formulation for higher production of Tor putitora. However, research on the same percentage of protein diet is recommended for yearlings.

Resumo Para o bom crescimento dos peixes, é necessário alimentá-los com uma alimentação adequada e balanceada. Um estudo foi realizado para descobrir o efeito de diferentes dietas à base de proteínas em alevinos de Tor putitora (mahseer). Foi preparado um alimento com níveis de proteína dietética de 35%, 40%, 45% e 50%. O efeito de diferentes alimentos à base de proteína no ganho de peso, taxa de crescimento padrão (SGR), taxa de conversão alimentar (FCR), ganho de peso percentual, eficiência de conversão alimentar (FCE) e taxa de eficiência proteica (PER) foi estudado. Foi observado um aumento na taxa de crescimento com um aumento na concentração de proteína de até 45%. Os alevinos alimentados com dieta de 45% de proteína apresentaram o maior crescimento, seguidos de 50%, 40% e 35%. O valor de SGR foi maior para dieta com 45% de proteína (8,56), seguido de 50% e 40%, enquanto os menores valores foram observados para ração com 35% de proteína (1,57). A mesma tendência foi observada para FCE. Os maiores valores de PER foram observados em peixes alimentados com 45% de ração à base de proteína (0,65), seguido por 50% (0,56), 40% (0,38) e 35% (0,17). A taxa de conversão alimentar foi menor para a dieta com 45% de proteína (3,41), enquanto a maior para a dieta com 35% de proteína (16,85). Concluiu-se que a dieta à base de proteína de 45% foi a melhor formulação alimentar para maior produção de Tor putitora. No entanto, a pesquisa sobre a mesma porcentagem de dieta proteica é recomendada para animais de um ano.

Effect of different protein based feed on the growth of mahseer / Efeito de alimentação baseada em proteínas diferentes no crescimento de mahseer

For the proper growth of fish, it is necessary to feed the fish with a proper and balanced diet. A study was conducted to find out the effect of different protein-based diets on fingerlings of Tor putitora (mahseer). A feed with dietary protein levels of 35%, 40%, 45%, and 50% were prepared. The effect of different protein-based feed on weight gain, standard growth rate (SGR), food conversion ratio (FCR), percent weight gain, food conversion efficiency (FCE), and protein efficiency ratio (PER) was studied. An increase was observed in the growth rate with an increase in protein concentration up to 45%. The fingerlings fed a 45% protein diet shown the highest growth, followed by 50%, 40%, and 35%. The SGR value was greatest for 45% protein diet (8.56) followed by 50% and 40%, while the least values were observed for 35% protein feed (1.57). The same trend was observed for FCE. The highest PER values was observed in fishes fed 45% protein-based feed (0.65) followed by 50% (0.56), 40% (0.38) and35% (0.17). The food conversion ratio was lowest for 45% protein diet (3.41), while the greatest for 35% protein feed (16.85). It was concluded that a 45% protein-based diet was the best feed formulation for higher production of Tor putitora. However, research on the same percentage of protein diet is recommended for yearlings.

Para o bom crescimento dos peixes, é necessário alimentá-los com uma alimentação adequada e balanceada. Um estudo foi realizado para descobrir o efeito de diferentes dietas à base de proteínas em alevinos de Tor putitora (mahseer). Foi preparado um alimento com níveis de proteína dietética de 35%, 40%, 45% e 50%. O efeito de diferentes alimentos à base de proteína no ganho de peso, taxa de crescimento padrão (SGR), taxa de conversão alimentar (FCR), ganho de peso percentual, eficiência de conversão alimentar (FCE) e taxa de eficiência proteica (PER) foi estudado. Foi observado um aumento na taxa de crescimento com um aumento na concentração de proteína de até 45%. Os alevinos alimentados com dieta de 45% de proteína apresentaram o maior crescimento, seguidos de 50%, 40% e 35%. O valor de SGR foi maior para dieta com 45% de proteína (8,56), seguido de 50% e 40%, enquanto os menores valores foram observados para ração com 35% de proteína (1,57). A mesma tendência foi observada para FCE. Os maiores valores de PER foram observados em peixes alimentados com 45% de ração à base de proteína (0,65), seguido por 50% (0,56), 40% (0,38) e 35% (0,17). A taxa de conversão alimentar foi menor para a dieta com 45% de proteína (3,41), enquanto a maior para a dieta com 35% de proteína (16,85). Concluiu-se que a dieta à base de proteína de 45% foi a melhor formulação alimentar para maior produção de Tor putitora. No entanto, a pesquisa sobre a mesma porcentagem de dieta proteica é recomendada para animais de um ano.

COVID-19 epidemic curve in Brazil: a sum of multiple epidemics, whose inequality and population density in the states are correlated with growth rate and daily acceleration. An ecological study

ABSTRACT Background: The epidemic curve has been obtained based on the 7-day moving average of the events. Although it facilitates the visualization of discrete variables, it does not allow the calculation of the absolute variation rate. Recently, we demonstrated that the polynomial interpolation method can be used to accurately calculate the daily acceleration of cases and deaths due to COVID-19. This study aimed to measure the diversity of epidemic curves and understand the importance of socioeconomic variables in the acceleration, peak cases, and deaths due to COVID-19 in Brazilian states. Methods: Epidemiological data for COVID-19 from federative units in Brazil were obtained from the Ministry of Health's website from February 25 to July 11, 2020. Socioeconomic data were obtained from the Instituto Brasileiro de Geografia e Estatística (https://www.ibge.gov.br/). Using the polynomial interpolation methods, daily cases, deaths and acceleration were calculated. Moreover, the correlation coefficient between the epidemic curve data and socioeconomic data was determined. Results: The combination of daily data and case acceleration determined that Brazilian states were in different stages of the epidemic. Maximum case acceleration, peak of cases, maximum death acceleration, and peak of deaths were associated with the Gini index of the gross domestic product of Brazilian states and population density but did not correlate with the per capita gross domestic product of Brazilian states. Conclusions: Brazilian states showed heterogeneous data curves. Population density and socioeconomic inequality were correlated with a more rapid exponential growth in new cases and deaths.