Mechanism of driver protein family member C1 in triple negative breast cancer / 国际生物医学工程杂志

Objective:To explore the function and role of kinesin family member C1 (KIFC1) in triple negative breast cancer (TNBC).Methods:The mRNA level of KIFC1 in TNBC tissues and normal tissues adjacent to cancer was analyzed by bioinformatics methods, and the relationship between its expression and the survival rate of thepatients was analyzed. The clinicopathological characteristics of 96 TNBC patients were retrospectively analyzed. Immunohistochemical method was used to detect the expression of KIFC1 in tumor tissues and normal tissues adjacent to cancer, and to analyze the expression of KIFC1 in TNBC patients and its relationship with clinicopathological characteristics.Results:The results of bioinformatics analysis showed that KIFC1 is highly expressed in TNBC tissue and is correlated with the patient's disease-free survival ( P<0.05). In TNBC tissue, the positive high expression rate of KIF23 is 58.3%, while it is mainly low or no expressionin adjacent tissues. The high expression of KIF23 is related to the tumor grade of TNBC patients ( P<0.05). The results of in vitro cell experiments show that knocking down KIFC1 can significantly reduce the colony forming ability and proliferation ability of TNBC cells. The results of in vivo experiments in mice showed that knocking down KIFC1 can significantly reduce tumor volume. Conclusions:KIFC1 can be used as a prognostic factor of TNBC. Low expression of KIFC1 can inhibit the proliferation of TNBC cells in vivo and in vitro. KIFC1 is expected to be a prognostic marker and therapeutic target for TNBC.

Expression of kinesin family member C1 in hepatocellular carcinoma and its correlation with prognosis and clinicopathological features / 国际生物医学工程杂志

Objective To study the expression of kinesin family member C1 ( KIFC1 ) in hepatocellular carcinoma (HCC) and analyze its correlation with the clinicopathological features and prognosis of patients. Methods The expression levels of KIFC1 protein in the HCC tissues from 82 patients were determined by immunohistochemical staining. The correlation between KIFC1 protein and clinicopathological characteristics (including age, gender, tumor nodules, tumor grade, tumor volume, lymph node metastasis, and alpha-fetoprotein expression) was analyzed. The Kaplan-Meier analysis was used to analyze the effect of KIFC1 expression level on overall survival and progression-free survival in patients with HCC. The expression level of KIFC1 mRNA in liver cancer tissue was analyzed by GPEIA database. The correlation between KIFC1 expression and prognosis was analyzed by KM-plotter. Results KIFC1 protein is significantly overexpressed in liver cancer tissues, and its expression level is significantly correlated with tumor nodule number (P=0.023) and tumor size (P=0.011). Patients with high expression of KIFC1 had poor overall disease and disease-free survival (all P<0.05). KIFC1 mRNA is significantly overexpressed in liver cancer tissues and correlated with disease-free survival and overall survival. Conclusions The expression of KIFC1 protein is highly expressed in liver cancer tissues, and its expression level is related to the clinicopathological characteristics of liver cancer. Bioinformatics analysis results show that KIFC1 is related to the poor prognosis of patients, suggesting that KIFC1 is expected to be a potential predictor and therapeutic target for liver cancer prognosis.