Hepatoprotective Activity of Calycopteris floribunda on Clopidogrel and Atorvastatin Induced Hepatic Damage in Rodents

Preclinical testing hepatoprotective potential of hydroalcoholic leaf extract of Calycopteris floribunda against liver damage caused by atorvastatin and clopidogrel. Atorvastatin and clopidogrel were delivered intraperitoneally for 14 days at 80mg/kg and 20mg/kg respectively, while leaf extract was given orally for the same period at 200mg/kg and 400mg/kg. To evaluate the impact of the leaf extract on the atorvastatin and clopidogrel-induced liver damage, many biochemical markers, Serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), total protein, and serum alkaline phosphatase were measured. Blood samples from mice given hydroalcoholic Calycopteris floribunda leaf extract showed a noteworthy decrease in serum indicators, suggesting that the leaf extract had an impact on the hepatocytes' ability to operate normally again. On the other hand, the mice receiving atorvastatin and clopidogrel treatment showed increased levels, which suggested serious liver injury. Silymarin (100 mg/kg) was given as a benchmark. The current investigation found that Calycopteris floribunda hydroalcoholic extract significantly protected the liver against atorvastatin and clopidogrel-induced hepatic damage.

AN OBSERVATIONAL STUDY OF DENGUE INDUCED HEPATIC DYSFUNCTION INA TERTIARY CARE HOSPITAL OF GKGH BHUJ

INTRODUCTION: Dengue infection is a major health problem worldwide including our country.Globally the incidence of Dengue has grown dramatically in the recent years. Every year during themonsoon months and later, many parts of the country witness outbreak of dengue infection. 2020was no exception and we experienced an outbreak of this vector borne disease in Bhuj. An analysisof these patients revealed that in addition to the classical features of fever, body ache, rash andthrombocytopenia and bleeding tendency, there were other features such as liver dysfunctionincluding a preferential rise of SGOT, hepatomegaly, splenomegaly, ascites, gallbladder wall edemaand pericholecystic fluid collection.OBJECTIVE: To study clinical, biochemical and radiological changes in the liver of patients withDengue fever.METHOD:observational and cross-sectional study was conducted on a 50 suspected cases of Dengue feveradmitted and diagnosed at GKGH hospital Bhuj in October 2020. Detailed history, clinicalexamination, biochemical parameters, radiological investigation for liver function was done in allpatients. All patients were treated as per NVBDCP guidelines.RESULTS: all patients in our study had hepatic dysfunction in the form of elevated SGOT abovenormal limits.CONCLUSIONS: Our study shows that there are certain features of Dengue that are not known tobe usually associated with it. The presence of raised liver enzymes in all patients (SGOT > SGPT),ascites, hepatomegaly, splenomegaly, and gallbladder edema and pericholecystic fluid collection.

Safety & Efficacy of a Fixed Dose Combination of Paracetamol (125 mg), Phenylephrine (2.5 mg) and Chlorpheniramine Maleate (1mg) [Flucold Drops] in the Treatment of Common Cold and Flu Syndrome in Children : Postmarketing Surveillance Study

Background :The common cold and flu syndrome primarily affects the upper respiratory tract, along with a low fever and some systemic symptoms such as sore throat, cough, nasal decongestion, headache, and so on. Several clinical studies have shown that combining analgesics, antihistaminics, and decongestants provides better symptom relief in the common cold. The current post-marketing surveillance study was designed to look into the safety and efficacy of commercially available Flucold Drops in the Indian population. Methodology :A current prospective, single arm, multicenter, post-marketing clinical study included 224 subjects, 220 of whom completed the study. All patients were given Flucold Drops for three days and then monitored for the next six days. During the study, the incidence of adverse events (AE) and serious adverse events (SAE) was assessed. The efficacy of the Flucold Drops was evaluated using VAS score changes from the beginning to the end of the treatment. The product’s safety was also evaluated using blood biomarkers such as haemoglobin, platelet count, SGOT, SGPT, and creatinine level. Results : Results show the reduction in symptomatic score of common cold and flu syndrome observed after 2rd follow-up visit (0.202+0.325 to 0.139+0.231). During the study, no intervention-related adverse events were observed. Furthermore, no Serious Adverse Events (SAE) were observed in the study or follow-up period. The study found no changes in the levels of blood biomarkers (haemoglobin, platelets, SGOT, SGPT, and creatinine). Conclusions : Flucold Drops are safe and effective in the treatment of common cold and flu syndrome in Children and infants.

A Study to Detect Liver Enzyme Dysfunction among Patients on First Line Anti-Tubercular Drugs from RNTCP during the Course of Anti-TB Treatment.

Globally, an estimated 10.0 million people developed Tuberculosis in 2017. Side effects and toxicity of the first line anti-tubercular drugs were hepatotoxicity, skin rash, and joint pain. If hepatotoxicity develops on reintroduction of treatment with the same regimen, then treatment should be started with hepato-safe regimen. Majority of the reports have used an elevated Alanine Transaminase (ALT) or Aspartate Transaminase (AST) of 3 times upper limit of normal range (ULN) with symptoms attributable to liver injury or 5 times ULN of ALT or AST without symptoms to define hepatotoxicity. Due to paucity of studies regarding adaptive response, this study was conducted to find out the proportion of anti-tubercular drug (ATD) induced hepatitis during Anti-TB treatment and frequency of adaptive changes in Liver Enzymes during the course of anti TB treatment.METHODS116 patients who were diagnosed to have Pulmonary (PTB)/ Extrapulmonary (EPTB) tuberculosis at Outpatient & In-Patients of Department of Chest Medicine, Burdwan Medical College & Hospital, for eleven months after fulfilling the inclusion and exclusion criteria were included in the study. Treatment was given as per guidelines of Revised National TB Control Program.RESULTSIn 83.3% patients only SGPT level was elevated, while in 71% SGOT was only elevated. Both SGPT and SGOT were elevated in 66.7% of cases. Only 1.8% cases were observed with elevated SGPT and SGOT on six occasions. Only 16.7% had no elevation in SGPT and 28.9% had no elevation in SGOT. Most of the patients had asymptomatic elevation of liver enzymes and didn’t need any treatment interruption. 4.38% patients developed drug induced hepatitis and needed treatment interruption for about two weeks and all were reintroduced with the same regimen successfully.CONCLUSIONSDrug induced liver function abnormality is a common occurrence during the course of anti-TB treatment. Most patients show tolerance to anti-TB drugs and get adjusted after transient rise in liver enzymes. Concomitant use of hepatotoxic agent should be avoided as far as possible.

Study of viral markers, clinical and biochemical profile of viral hepatitis in patients of alcoholic liver disease

Background: There is a significant worldwide burden of Alcoholic Liver Disease (ALD). Both alcohol abuse and infection with hepatitis viruses can lead to liver disease. Alcohol and hepatitis viruses have synergistic effects in the development of liver disease. Thus, early detection of virus hepatitis and targeted interventions can improve prognosis in ALD.Methods: This cross-sectional study was conducted among 180 patients coming to Baroda medical college and SSG hospital, Vadodara having alcoholic liver disease were studied and evaluated for markes of viral hepatitis and its clinical and biochemical profile in alcoholic liver disease.Results: our study we had taken 180 patients of alcoholic liver disease out of which male were 92% and female were 8%. Prevalence of viral hepatitis was 27.7% in ALD patients. Out of which hepatitis E was 13% followed by hepatitis A 11%, hepatitis B 4.44% and least was Hepatitis C 0.5%. In clinical profile fever was significantly higher in patients of viral hepatitis with ALD than patients without viral hepatitis. Bilirubin was not significant differ in both groups of patients but SGOT and SGPT had higher values in patients of viral hepatitis with ALD and thus ratio of SGOT/SGPT was also affected due to higher value of SGOT and SGPT.Conclusions: Alcohol consumption and hepatitis virus infection have a synergic hepatotoxic effect, and the coexistence of these factors increases the risk of advanced liver disease. Patients starting treatment for chronic viral hepatitis infection should be specifically advised to stop or reduce alcohol consumption because of its potential impact on treatment efficacy and adherence and may benefit from additional support during antiviral therapy specially in chronic hepatitis

Comparative Analysis of Indices of the Liver Function Tests in Patients with and without Non- Alcoholic Fatty Liver Disease at a Teaching Hospital in Hapur

Background: Non-Alcoholic fatty liver disease (NAFLD) has become a worldwide health concern with increase in the global incidence of obesity and it is now considered the hepatic component of the metabolic syndrome. Aims and Objective: The study’s aim was to compare the indices of the Liver Function tests in compensated chronic liver disease patients with and without NAFLD. Materials and Methods: A total of 100 consecutive patients with compensated chronic liver disease were recruited into the study. A structured questionnaire was administered to obtain relevant socio-demographic data. NAFLD was diagnosed based on clinical, biochemical, ultrasonographic and in a few histological features. The Adult Treatment Panel III criteria were used to identify patients with the metabolic syndrome. Results: In our study, 100 participants were recruited into study. 40 out of 60 patients (67%) showed grade-1 fatty liver findings and 20 out of 60 patients (33%) showed grade-2 fatty liver. Approximately 19% had fatty liver finding. The mean (SD) age of persons with NAFLD was 45.12 (±8.07) years compared  to 47.49(±11.79) years for persons without NAFLD. The difference was not statistically significant (p=0.2). Body mass index (BMI), central obesity (waist circumference), fasting  blood sugar, blood pressure, total cholesterol and triglycerides were significantly higher in  the NAFLD group (p= <0.05) respectively.  Conclusion: Indices of the deranged Liver functions were more prevalent in persons with NAFLD. It is recommended that patients with NAFLD be screened for metabolic syndrome and appropriate therapy instituted to decrease the risk of both hepatic and cardiovascular complications.

Relationship Between The Ejection Fraction In Echocardiography With Liver Function In Heart Failure Patients

Background: Heart failure (HF) is a complex clinical syndrome, which is based on the inability of the heart to pump blood throughout the body adequately, due to structural and functional disorders of the heart. The most common cause of left ventricular myocardial dysfunction. Heart failure is described by the measurement of left ventricular (LV) ejection fraction (LV). Heart failure with decreased ejection fraction (HFrEF) is characterized by LVEF ≤40%; preserved EF (HFpEF) is characterized by LVEF ≥50% assessed by dopplerococardiography. In heart failure, the heart cannot supply oxygen adequately, so it can cause damage to other organ systems, such as the liver. It is important to identify from the outset of liver biochemical abnormalities in patients with heart failure, because the examination is useful in assessing the severity and duration of heart failure. Aim:To determine the relationship between ejection fraction on echocardiography and liver function in heart failure patients. Method: This cross-sectional study was conducted at the H. Adam Malik General Hospital in Medan in June 2017 until the research sample was fullled. Heart failure patients performed echocardiography and blood tests: SGOT, SGPT, Bilirubin, ALP, GGT, Albumin. Data analysis (Chisquare / sher exact test) using SPSS. Result: Subjects were male 36 patients (69.2%), and women 16 patients (30.8%), with an average age of 52.08 ± 10.52 years. The frequency of increasing total bilirubin and GGT is greater than for other liver functions. Liver function characteristics of SGOT, SGPT, total bilirubin, ALP, and GGThad a higher mean of ejection fraction <= 40% compared to ejection fraction> 40%. With the analysis of the Fisher Exact test p value <0.05 for the relationship of Ejection Fraction to total bilirubin (p = 0.001) and GGT(p = 0.016). Conclusion:There is a relationship between ejection fraction and liver function and what is meaningful is total bilirubin with GGT.

Clinical and biochemical profile of malaria: retrospective observational study from a tertiary centre

Background: Malaria is one of the common causes of acute febrile illness in tropical countries. Malaria presents with varied manifestations. This retrospective study carried to know the clinical profile and laboratory abnormalities seen in malaria patients.Methods: The data was collected retrospectively from 1st January to 31st December 2017. Inclusion criteria: all fever cases above 15 years of age of both the sexes diagnosed as malaria by peripheral smear examination and malaria card test. Exclusion criteria: combined malaria with other fevers such as dengue, chikangunya. Fever cases negative for malaria tests. Malaria cases with history of chronic kidney disease, chronic liver disease such as cirrhosis of liver, chronic viral hepatitis, liver abscess, and chronic illness such as rheumatoid arthritis, diabetes, and hypertension. The data regarding the clinical presentation of patients and laboratory values such as hemoglobin, total leukocytecount, platelet count, total bilirubin, SGOT, SGPT, albumin values collected and analyzed with tables and percentage.Results: A total of 57 malaria cases were analyzed, 71.9 % males, 28.1% were females. The commonest age group was between 15- 30 years (61.4%). 29 patients (50.9%) had P. vivax, 20 patients (35.1%) P. falciparum and 8 patients (14%) mixed infection. The most common clinical presentation was fever with chills (100%) followed by vomiting (68.4%), splenomegaly (56.1%), headache (45.6%), pain abdomen (43.9%).19 cases (33.3%) had hemoglobin less than 10gm/dl; 42 cases (73.6%) had thrombocytopenia; 46 cases (80.7%) had urea ≥30mg/dl; 14 cases (24.6%) had creatinine ≥1.4; 26 cases (45.6%) had total bilirubin >1.2mg/dl ; 17 cases (29.8%) had SGOT >45 IU; 33 cases (57.9%) had SGPT > 45 IU and 32 cases (56.1%) had albumin level ≤3.5gm/dl.Conclusions: In the study malaria due to P. vivax was more common than P. falciparum, malaria affected young adults, males more than females. Reduced hemoglobin and platelet count, deranged liver and renal function and reduced serum albumin seen commonly in malaria.

Synergistic protective effect of picrorhiza with honey in acetaminophen induced hepatic injury.

Rhizome of picrorhiza along with honey prevents hepatic damage and cure the acetaminophen (paracetamol) induced hepatotoxicity by modulating the activity of hepatic enzymes. Here, we studied the in vivo effects of Picrorhiza kurroa and honey on acetaminophen induced hepatotoxicity Balb/c mice model. Hepatic histopathological observations of acetaminophen fed (day-6) group showed more congestion, hemorrhage, necrosis, distorted hepatic architecture and nuclear inclusion. Such damages were recompensed to normal by picrorhiza or honey alone or both in combinations. We observed increased activity of SGPT and SGOT in injured liver tissues, and that too was compensated to normal with picrorhiza or honey alone or both in combinations. We observed 1.27 and 1.23-fold enhanced activity of SGPT in serum and liver lysate, respectively while SGOT showed 1.66 and 1.11 fold enhanced activity. These two enzymes are signature enzymes of liver damage. Thus, our results support that honey may be used with drug picrorhiza due to its synergistic role to enhance hepatoprotective and hepatoregenerative ability along with allopathic drugs to mitigate the hepatotoxic effects.

Combination of Loperamide and Niacin modify DENA induced altered Liver enzymes .

Hepatocellular Carcinoma (HCC) is among the most lethal cancers which makes it the third most frequent cause of cancer related deaths. Diethylnitrosamine (DENA) is a potent initiator and hepatocarcinogen in rats. DENA induced Hepatocellular damage clearly demonstrates by the elevated levels of liver enzymes serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase (ALP), and α-feto protein (AFP). This work is an attempt to test the hypothesis that Loperamide (5mg/kg) and Niacin in combination restores the DENA (160mg/kg) induced altered enzymes after single i.p administration in Wistar rats. The ability to alter the enzymes was measured by comparing biochemical serum markers and AFP. The results have confirmed the significant elevation of these parameters in DENA control group compared to normal control and the therapeutic groups. Therapeutic group significantly reveals that Loperamide and Niacin restores the altered hepatic enzymes towards the Normal. Key messages: Our data reveals and confirms that this remarkable combination possess the potential for the treatment of hepatocellular carcinomas in rats exposed to DENA. Administration of Loperamide + Niacin relatively improved the biochemical parameters to values approximating those of the normal controls

Effect of Kasni seed preparations on serum glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels in newly diagnosed patients of type 2 diabetes mellitus.

Background: Kasni (Cichorium intybus L.) reported to play an important role in the effective management of serum liver enzymes SGPT & SGOT in various animal models and this study is extension to newly diagnosed patients of type 2 diabetes mellitus. Methods: Newly diagnosed 90 patients of Type2 DM, age 35-65years, of either sex were divided into 3 groups. In group I only Metformin sustained release once a day and in group II/III 6 grams crude seed powder or 50 ml decoction of crude seed powder was given twice a day for 90 days in combination with Metformin sustained release orally once in a day. Serum liver enzyme levels of SGPT & SGOT were measured at zero, 30th, 60th and 90th day. Results: All the three groups showed a significant reduction in SGPT & SGOT across the four time periods. Post hoc Tukey HSD test shown that there was a significant difference between group I & II (p=0.011) and group I & III (p=0.000) for SGPT and group I & II (p=0.012) and group I & III (p=0.000) for SGOT. Conclusions: The add on therapy with Kasni seed preparations is more effective for the management of altered SGPT and SGOT levels in Type2 diabetes mellitus patients than only oral hypoglycaemic agent in decreasing SGPT & SGOT of selected patients. Among Kasni seed preparation treated groups, decoction was found more effective than crude seed powder.

Variation of CD4+ T-Lymphocyte Counts and Transaminases in HIV and HIV/HBV Co-infected Patients on Therapy at Nylon Hospital Douala, Cameroon.

Objective: This study was aimed at determining the prevalence of Hepatitis B and associated risk factors such as CD4+ counts variation and liver enzymes among HIV co– infected patients and those with HIV mono-infections only. Design and Methods: Three hundred and fourteen (314) HIV patients took part in this cross sectional case control study. Socio-demographic information and history of exposure to risk factors such as scarification, blood transfusion, and unprotected sexual intercourse and alcohol consumption, were obtained through a well-structured questionnaire. Serological tests were done to determine the presence of Hepatitis B (HB) surface Antigen, liver enzymes’ activities were estimated and CD4+ cell counts evaluated using standard laboratory methods. Results: Out of the 314 HIV patients, 20 (6.4%) tested positive for hepatitis B surface antigen (HBsAg) while 294 (93.6%) were negative. Most HIV patients co–infected with HBV were in the age group 31 to 45 years. There was no significant variation when co-infection and mono-infection groups were compared based on age and sex (p=0.7405 and p=0.3361). More males, 7 (2.23%) against 2 (0.64%) females (P=0.02) co–infected with HBsAg had a CD4+ cell counts in the range 201-350cells/µL. No significant difference of liver transaminases (SGPT and SGOT) levels between mono and co-infection groups (P>0.05) was observed. No association of HBsAg with observed risk factors among HIV patients was noted. Conclusion: The study concluded that the prevalence of hepatitis B among HIV patients was 6.4% with majority of the patients having CD4+ cell counts within 201-350. The liver function parameters (transaminases) were not affected with HIV/HBV co-infection.

Hepatotoxicidad aguda de la decocción de la planta Mimosa púdica en ratas Sprague Dawley / Acute Hepatotoxicity of Mimosa pudica Plant Decoction in Sprague Dawley Rats

Introducción: la enfermedad hepática tóxica es un problema desconcertante debido al amplio número de sustancias capaces de inducir este tipo de reacciones. Datos epidemiológicos y estadísticos demuestran que la Mimosa púdica conocida como moriviví, sensitiva, adormidera es una de las plantas (objeto de abuso) más empleadas como adulterante en los pitillos de marihuana en pacientes drogadictos. Objetivos: evaluar el comportamiento de los parámetros bioquímicos y describir los hallazgos anatopatológicos en el hígado de ratas por efecto de la planta Mimosa púdica. Métodos: se realizó un estudio preclínico de hepatotoxicidad aguda con la planta Mimosa púdica en animales de experimentación. Se seleccionaron 25 ratas Sprague Dawley, cinco por cada grupo (control positivo, control y tres grupos experimentales) de experimentación. Se administró una dosis de 500, 1000 y 2000 miligramos por kilogramo de peso corporal de la decocción de la planta a los grupos experimentales respectivamente; tetracloruro de carbono a dosis de 3 mililitros por kilogramo de peso corporal al control positivo y agua estéril al control. Resultados: aumento de los niveles de transaminasas (TGP y TGO) en los grupos experimentales con respecto al grupo control. Las alteraciones anatomopatológicos observadas fueron: necrosis focal periportal, congestión de venas centrolobulillares y degeneración acidófila del hígado. Conclusiones: el consumo de la decocción de la planta Mimosa púdica afecta el hígado, lo que provoca alteraciones bioquímicas y anatomopatológicas, de probable importancia en pacientes drogadictos, aunque se requieren más estudios.

Introduction: toxic liver disease is a concerning problem due to the large number of substances that cause such reactions. Epidemiological and statistical data show that Mimosa (Moriviví), known as sensitive and opium poppy is one of the most used plants by drug-addict patients, as an adulterant in marihuana cigarettes drug. Objectives: to evaluate the behavior of biochemical parameters and describe pathological findings in the liver of rats as a result of Mimosa pudica plant. Methods: a preclinical study of acute hepatotoxicity with Mimosa pudica plant in experimental animals was performed. Twenty five Sprague Dawley rats, five per group (positive control, group control and three experimental) were selected for the study. A dose of 500, 1000 and 2000 mg administered per kilogram of body weight of the decoction of the plant to the experimental groups respectively; carbon tetrachloride at doses of 3 milliliters per kilogram of body weight to the positive control and sterile water to control. Results: the results showed an increase of levels of transaminases (SGOT and SGPT) in the experimental groups compared to the control group. The pathological changes observed were focal periportal necrosis, congestion and centrilobular veins acidophilic degeneration of the liver. Conclusions: the consumption of the decoction of the plant Mimosa pudica affects the liver, causing biochemical and pathological alteration of likely importance in drug patients, although further studies are required.

Effect of Dimethoate and Chlorpyrifos in Hepatic and Renal Function of People Belonging to Risk Groups in Iraklia Serres (N. Greece).

Aims: There is little evidence concerning the effects of organophosphates in the liver of healthy individuals, and the existing researches come to contradictive results. In this study, we evaluated the influence of organophosphates (Dimethoate, Chlorpyrifos) in liver and renal function of healthy exposed workers, not experiencing symptoms of serious intoxication Study Design: Measure serum activity of the liver function monitoring enzymes SGPT, SGOT, γ-GT and ALP and serum concentration of the renal function indicative biomarkers urea and creatinine. Place and Duration of Study: Sample were collected in Health Care Greece of Iraklia Serres and analyzed in Department of Medical Laboratory Studies Alexander Technological Educational Institute of Thessaloniki. Methodology: Blood samples were collected from 112 individuals, randomly selected from villagers of N. Greece. 42 of them were organophosphates (OP) applicators aged less than 50 years old (mean age 37 years old) and 42 were OP applicators older than 50 years old (mean age 58 years old); while 28 individuals (13 of them were less than 50 years old and 15 older than 50 years) were not OP applicators and used as control groups. Results: A remarkable and statistically significant increase (P < 0.05) in the main liverfunction monitoring enzymes (SGOT, SGPT, γ-GT) was observed in exposed people compared to the control group. Increase in ALP values compared to not exposed individuals was not observed. Concerning the kidneys, data analysis shows that there is not any significant effect on their operation by the use of OP. Conclusion: The age of OP applicators and the time past between the application and the measure of blood serum seems to play an important role in the values of hepatic enzymes. While the renal indicators seemed not so much affected, as organophosphates are rapidly metabolized in human organism.

Antidiabetic and antihyperlipidaemic activity of ethanol extract of Melastoma malabathricum Linn. leaf in alloxan induced diabetic rats

Objective: To evaluate the antidiabetic and antihyperlipidaemic effect of ethanol extract ofMelastoma malabathricum (M. malabathricum) Linn. leaf in alloxan induced diabetic rats. Methods: Diabetes was induced in albino rats by administration of alloxan monohydrate (150 mg/kg i.p). the ethanol extracts of M. malabathricum at a dose of 150 and 300 mg/kg of body weight were administrated at a single dose per day to diabetes induced rats for a period of 14 d. The effect of ethanol extract of M. malabathricum leaf extract on blood glucose, plasma insulin, creatinine, glycosylated haemoglobin, urea serum lipid profile [total cholesterol, triglycerides, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and phospholipid, serum protein, albumin, globulin, serum enzymes (serum glutamate pyruvate transaminases), serum glutamate oxaloacetate transaminases, and alkaline phosphatase] were measured in the diabetic rats.Results:In the acute toxicity study, ethanol extract of M. malabathricum leaf was non-toxic at 2 000 mg/kg in rats. The increased body weight, decreased blood glucose, glycosylated haemoglobin and other biochemical parameters level were observed in diabetic rats treated with both doses of ethanol extract of M. malabathricum leaf compared to diabetic control rats. In diabetic rats, ethanol extract of M. malabathricum leaf administration, altered lipid profiles were reversed to near normal than diabetic control rats.Conclusions:Ethanol extract of M. malabathricum leaf possesses significant antidiabetic and antihyperlipidaemic activity in diabetic rats.

Antidiabetic and antihyperlipidaemic activity of ethanol extract of Melastoma malabathricum Linn. leaf in alloxan induced diabetic rats

<p><b>OBJECTIVE</b>To evaluate the antidiabetic and antihyperlipidaemic effect of ethanol extract of Melastoma malabathricum (M. malabathricum) Linn. leaf in alloxan induced diabetic rats.</p><p><b>METHODS</b>Diabetes was induced in albino rats by administration of alloxan monohydrate (150 mg/kg i.p). the ethanol extracts of M. malabathricum at a dose of 150 and 300 mg/kg of body weight were administrated at a single dose per day to diabetes induced rats for a period of 14 d. The effect of ethanol extract of M. malabathricum leaf extract on blood glucose, plasma insulin, creatinine, glycosylated haemoglobin, urea serum lipid profile [total cholesterol, triglycerides, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, high density lipoprotein-cholesterol and phospholipid, serum protein, albumin, globulin, serum enzymes (serum glutamate pyruvate transaminases), serum glutamate oxaloacetate transaminases, and alkaline phosphatase] were measured in the diabetic rats.</p><p><b>RESULTS</b>In the acute toxicity study, ethanol extract of M. malabathricum leaf was non-toxic at 2 000 mg/kg in rats. The increased body weight, decreased blood glucose, glycosylated haemoglobin and other biochemical parameters level were observed in diabetic rats treated with both doses of ethanol extract of M. malabathricum leaf compared to diabetic control rats. In diabetic rats, ethanol extract of M. malabathricum leaf administration, altered lipid profiles were reversed to near normal than diabetic control rats.</p><p><b>CONCLUSIONS</b>Ethanol extract of M. malabathricum leaf possesses significant antidiabetic and antihyperlipidaemic activity in diabetic rats.</p>

Effect of Melia azedarach and Dodonaea viscosa aqueous leaf extracts on fertility in male albino rats.

Medicinal plants play a key role in human life as they are helpful in curing several diseases. They not only support health by the pharmacological nature but also utilizable as contraceptive options. The present study reveals that the medicinal plants Melia azedarach and Dodonaea viscosa leaf extracts showing antifertility activity. The decreased sperm count and reproductive organ weights including the necrotic changes in the seminiferous tubules of testis suggesting the antifertility activity of the plants. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and other serological studies were also carried out to know whether side-effects of the extracts.

Protective effect of -tocopherol against hematotoxicity, hepatotoxicity and nephrotoxicity induced by nickel sulfate in male albino rats.

Among the chemical hazards, heavy metal like nickel (Ni) is considered to be a serious one. It induces severe liver and kidney damage by altering several marker enzymes and ascorbate-cholesterol metabolism. The objective of the study was to investigate the possible protective role of α-tocopherol on NiSO4 (Ni II) exposed alteration of hematological parameters, markers of liver and kidney functions, hepatic and renal antioxidant defense system in male albino rats. We have studied the effects of α-tocopherol supplementation on nickel sulfate induced alteration of body weight, hematology, liver and kidney toxicity markers (SGOT, SGPT, total protein, urea, creatinine) and hepatic and renal antioxidant defense system of male albino rats. Nickel toxicity results in decreased body weight gain and relative liver and kidney weight. Nickel treatment also resulted in alteration of hematological parameters along with increased liver and kidney toxicity markers. Nickel sulfate administration significantly increased the level of lipid peroxides and decreased antioxidant enzyme activities in hepatic and renal tissue. Simultaneous treatment with á-tocopherol exhibited a possible protective role on the toxic effect of nickel on body and organ weights, hematological parameters, SGPT activity and improved tissue antioxidant defense system. α-tocopherol, may partially prevent nickel induced alteration of hematological and biochemical parameters as well as have amelioratic effects on nickel induced alteration of antioxidant status of liver and kidney.

Hepatoprotective activity of Adina cordifolia against ethanol induce hepatotoxicity in rats.

The acetone (AEAC) and aqueous extracts (AQEAC) of Adina cordifolia, belonging to the family Rubiaceae, were studied for hepatoprotective activity against Wister rats with liver damage induced by ethanol. It was found that AEAC and AQEAC, at a dose of 500 mg/kg body weight exhibited hepatoprotective effect by lowering the Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), alkaline phosphate and total bilirubin to a significant extent and also significantly increased the levels of total protein. The hepatoprotec-tive activity was also supported by histopathological studies of liver tissue. Since results of biochemical studies of blood samples of ethanol treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by ethanol and blood samples from the animals treated with AEAC and AQEAC showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells against ethanol induced hepatocellular injury. The effects of AEAC and AQEAC were comparable with standard drug silymarin.

Hepatoprotective activity of methanolic extract of rhyncosia beddomei baker leaves against carbon tetrachloride induced hepatotoxicity.

Rhyncosia beddomei Baker commonly known as Adavi-kandi, Vendiaku in Telugu belongs to the family Fabaceae.In the present study, the methanolic extract of Rhyncosia beddomei leaves was evaluated for its hepatoprotective effect against CCl4 induced hepatic injury in rats. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, ALP, triglycerides, bilirubin, total proteins and liver weight were tested in both treated and untreated groups. CCl4 (1ml/kg) enhanced the SGPT, SGOT, ALP, triglycerides, liver weight and reduced total proteins significantly. Treatment with methanolic extract of Rhyncosia beddomei leaves (200mg/kg and 400mg/kg) has brought back the altered levels of altered levels of biochemical markers significantly to the near normal levels in the dose dependant manner. Histopathological studies supported the hepatoprotective activity of Rhyncosia beddomei Baker.