RÉSUMÉ
BACKGROUND Plant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation. The present study focuses on the multiple shoot induction and proliferation of Ficus carica var. Black Jack. Factors that influence the growth of in vitro multiple shoots on the apical buds, which include growth media and PGRs, were investigated in this study. Different concentrations of cytokinins like 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (Kin) were used on woody plant medium (WPM) for the optimization of media for multiple shoot induction and proliferation. RESULTS Apical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots. The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 mM BAP. Proliferated multiple shoots were efficiently rooted using WPM + 20 mM BAP + 8 mM indole-3-acetic acid (IAA). This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack. CONCLUSIONS In the present study the observations indicate that WPM supplemented with 20 mM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack, and this technique can be potentially applied for commercialization of the plant
Sujet(s)
Ficus/embryologie , Organogenèse , Facteur de croissance végétal , Écorce/embryologieRÉSUMÉ
The active ingredients of Ficus hirta and Hypericum perforatum were collected from Traditional Chinese Medicine Database and Analysis Platform(TCMSP) and related papers. The potential targets of these two medicinal herbs were searched from HERB database, and those associated with microvascular angina were screened out from GeneCards, Online Mendelian Inheritance in Man(OMIM), Therapeutic Target Database(TTD), and HERB. Cytoscape was used to construct a protein-protein interaction(PPI) network of the common targets shared by the two herbs and microvascular angina based on the data of String platform. Metascape was employed to identify the involved biological processes and pathways enriched with the common targets. Cytoscape was used to draw the "active ingredient-target-pathway" network. AutoDock Vina was used to dock the core ingredients with the key targets. A total of 19 potential active ingredients and 71 potential targets were identified to be associated with microvascular angina. Bioinformatics analysis showed that phosphatidylinositol-3-kinase/protein kinase B(PI3 K-AKT), interleukin-17(IL17), hypoxia-inducible factor 1(HIF-1) and other signaling pathways were related to the treatment of microvascular angina by F. hirta and H. perforatum. Molecular docking results showed that β-sitosterol, luteolin and other ingredients had strong affinity with multiple targets including mitogen-associated protein kinase 1(MAPK1), epidermal growth factor receptor(EGFR) and so on. These findings indicated that F. hirta and H. perforatum may regulate PI3 K-AKT, IL17, HIF-1 and other signaling pathways by acting on multiple targets to alleviate oxidative stress, inhibit inflammatory response, regulate angiogenesis, and improve vascular endothelium and other functions. This study provides reference for in vitro and in vivo studies of the treatment of microvascular angina.
Sujet(s)
Humains , Médicaments issus de plantes chinoises/pharmacologie , Ficus , Hypericum , Médecine traditionnelle chinoise , Angor microvasculaire , Simulation de docking moléculaire , Pharmacologie des réseauxRÉSUMÉ
The application of plant growth regulators can promote better development of the seedlings, essential for the success of fruit growing. The current study aims to evaluate the use of plant growth regulators in fig tree seedlings 'Roxo de Valinhos'. The experiment was carried out in a suspended nursery covered with plastic, using rooted cuttings of the cultivar Roxo de Valinhos. Four applications were made with a commercial product, known as Stimulate®, at doses of 0, 50, 100, 150, and 200 mL L-1. Using a randomized block experimental design, the study was subdivided into plots (doses X days of the last application [after 0, 7, 14 and 21]). The following traits were evaluated: stem and root length; stem diameter; root volume; leaf number; leaf area; stem, leaf and root dry mass; specific leaf area, leaf weight ratio; leaf area ratio; and chlorophyll total. Results indicated that the product promoted greater seedlings development at a dose range from 100 to 150 mL L-1. Different dose levels did not vary according to the days after product application. Furthermore, using such technique enabled seedlings of higher quality and vigour.
A aplicação de reguladores vegetais pode configurar uma técnica viável para promoção do melhor desenvolvimento das mudas, imprescindível para o sucesso da fruticultura. Deste modo, objetivou-se avaliar o uso de reguladores vegetais na produção de mudas da figueira 'Roxo de Valinhos'. O experimento foi realizado em viveiro suspenso com cobertura plástica, utilizando-se estacas enraizadas da cultivar Roxo de Valinhos. Foram realizadas quatro aplicações com o produto comercial Stimulate® nas concentrações de 0, 50, 100, 150, e 200 mL L-1. Utilizou-se o delineamento experimental em blocos casualizados com parcelas subdivididas [concentrações X épocas de avaliação (após 0, 7, 14 e 21 dias da última aplicação)]. Avaliou-se: comprimento do ramo e da maior raiz; diâmetro do ramo; volume de raízes; número de folhas e área foliar; massa seca de folhas, ramos e raízes; área específica foliar, razão de peso foliar e razão da área foliar; e teores de clorofila total. O uso do produto, nas concentrações de 100 a 150 mL L-1, promoveu maior desenvolvimento das mudas. O efeito das diferentes doses não variou em função dos dias após aplicação do produto. O uso de tal técnica permite a obtenção de mudas de maior qualidade e vigor.
Sujet(s)
Ficus , Cytokinine , Tocophérols , GibbérellinesRÉSUMÉ
Introducción Los liposarcomas son lesiones que se originan en el tejido mesodérmico, su localización paratesticular es muy rara. Se divide en 4 subtipos histológicos, siendo el mixoide el más infrecuente. Tiene una recurrencia del 70%, Por lo que su tratamiento inicial debe ser agresivo. Objetivo resaltar su gran tamaño y que sea considerado como diagnóstico diferencial de masa inguinoescrotal. Presentación del caso Paciente masculino de 87 años con masa dolorosa inguinoescrotal derecha con diagnóstico de hernia inguinal unilateral, se realiza orquiectomía inguinal derecha. En patología se recibe pieza quirúrgica de 2440 g; al estudio histológico se observa lesión tumoral mesenquimal maligna, rodeado por estroma nodular mixoide prominente. Se diagnostica liposarcoma mixoide de cordón espermático. (Figura 2). Conclusiones Son neoplasias raras mal diagnosticadas como hernia inguinal, escrotal o lipoma de la médula espermática. Tiene un alto riesgo de metástasis, siendo la orquiectomía inguinal con resección del cordón espermático el manejo quirúrgico ideal.
Introduction Liposarcomas are lesions originated in the mesodermal tissue, paratesticular location is very rare. It is divided into 4 histological subtypes, being the most uncommon the myxoid. It has a recurrence of 70%, so the treatment should be aggressive. Objective to emphasize the large size and the differential diagnosis of inguinoescrotal mass. Case Presentation 87-year-old male patient with right inguinoescrotal pain with diagnosis of unilateral inguinal hernia, right inguinal orchiectomy is performed. A surgical specimen of 2440 g is received in pathology. Histological study shows malignant mesenchymal tumor lesion, surrounded by prominent myxoid nodular stroma. Myxoid liposarcoma of the spermatic cord is diagnosed. (Fig. 2). Conclusions they are rare neoplasms and are poorly diagnosed as inguinal hernia, scrotal or lipoma of the spermal cord. It has a high risk of metastasis, the ideal surgical management is an inguinal orchiectomy with resection of the spermatic cord.
Sujet(s)
Humains , Mâle , Sujet âgé de 80 ans ou plus , Cordon spermatique , Liposarcome myxoïde , Liposarcome , Thérapeutique , Orchidectomie , Ficus , Diagnostic différentiel , Métastase tumoraleRÉSUMÉ
Abstract Haemonchus contortus is a gastrointestinal nematode that is responsible for high mortality rates in ruminant herds. The resistance of nematodes to synthetic anthelmintics is widespread and requires a continuous search for new bioactive molecules, such as proteins. The objective of this study was to evaluate the anthelmintic potential of a protease purified from the latex of Ficus benjamina against H. contortus . Fresh latex was collected from plants via small incisions in the green stems, the rubber was removed by centrifugation, and the latex protein extract (LPE) was obtained. After LPE fractionation with ammonium sulfate and chromatography of the fraction containing the highest proteolytic activity on CM-cellulose, a cysteine protease (FbP) was purified. FbP has a molecular mass of approximately 23.97 kDa, and its proteolytic activity was stable between pH 6.0 and pH 10 and over a broad temperature range, with optimum activity at 60 °C. FbP inhibited both the development and exsheathment of H. contortus larvae, with 50% effective concentrations of 0.26 and 0.79 mg/mL, respectively. We conclude that this cysteine protease from F. benjamina latex with anthelmintic activity against H. contortus could be a promising alternative for the development of products for use in parasite control programmes.
Resumo Haemonchus contortus é um nematoide gastrintestinal, responsável por altas taxas de mortalidade em rebanhos de pequenos ruminantes. A resistência dos nematoides aos anti-helmínticos sintéticos está generalizada e requer uma busca contínua por novos compostos bioativos, como as proteínas. O objetivo deste trabalho foi avaliar o potencial anti-helmíntico da protease purificada do látex de Ficus benjamina contra H. contortus . O látex fresco foi coletado das plantas por pequenas incisões nas hastes verdes e o extrato proteico de látex (EPL) foi obtido. Após o fracionamento do EPL com sulfato de amônio e cromatografia da fração contendo a maior atividade proteolítica da CM-Celulose, uma protease cisteínica (FbP) foi purificada. A FbP tem massa molecular de cerca de 23,97 kDa, a atividade proteolítica foi estável entre pH 6,0 e pH 10 e ao longo de uma ampla faixa de temperatura, com atividade ótima a 60 °C. A FbP inibiu tanto o desenvolvimento quanto o desembainhamento das larvas de H. contortus, com 50% de inibição nas concentrações de 0,26 e 0,79 mg/mL, respectivamente. Concluímos que esta protease cisteínica do látex de F. benjamina, com ação anti-helmíntica contra H. contortus, pode ser uma alternativa promissora para o desenvolvimento de produtos a serem utilizados em programas de controle de parasitos.
Sujet(s)
Animaux , Extraits de plantes/pharmacologie , Ficus/composition chimique , Cysteine proteases/pharmacologie , Haemonchus/effets des médicaments et des substances chimiques , Latex/composition chimique , Anthelminthiques/pharmacologie , Ovis/parasitologie , Tests de sensibilité parasitaire , Électrophorèse sur gel de polyacrylamide , Cysteine proteases/isolement et purificationRÉSUMÉ
BACKGROUND: Carissa bispinosa, Ficus sycomorus, and Grewia bicolar are edible fruit plants that grow in the wild. The plants produce yellow-, red-, and purple-colored fruits and thus can be good sources of flavonoids for fighting oxidative reactions in humans, food, and the pharmaceutical industry. The present study aimed at isolating flavonoids from C.bispinosa, F. sycomorus, and G. bicolar fruits and determining their antioxidant activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2-azino-bis 3-ethylbenz-thiaz-oline-6-sulfonic acid (ABTS) model radical assays. METHODS: Analytical and preparative thin-layer chromatography was used to isolate flavonoids from the fruits using methanol/chloroform/hexane (7:2:1,v/v/v) as a mobile phase system. The ABTS and DPPH radical scavenging methods were used to test for the antioxidant activity of the samples, using quercetin and catechin as reference standards. RESULTS:Thin-layer chromatographic profiling revealed two different types of flavonoids from each plant.C. bispinosa yielded two flavonoid bands, Rfvalues 0.11 and 0.38;G. bicolaryielded two flavonoid bands,Rfvalues 0.63 and 0.81; andF.sycomorus also yielded two types of flavonoids, Rfvalues 0.094 and 0.81. All the extracted flavonoids exhibited significant antioxidant activity of over 80% at a concentration of 200 mg/L. The order of radical scavenging activity for the 200-mg/Lsamples is G. bicolar Rf(0.81) >C. bispinosa Rf(0.113) >F. sycomorus Rf(0.094) >F. sycomorus Rf(0.047) >C. bispinosa Rf(0.38) >G. bicolar Rf(0.63).G. bicolar(Rf= 0.81) exhibited antioxidant activitythat was superior to that of catechin. CONCLUSION:The present study results show that C. bispinosa,F. sycomorus,and G. bicolar contain different flavonoid types with significant antioxidant activity of over 80% at a concentration of 200 mg/L. Therefore, the fruits can be used as a source of natural antioxidants which can be used as nutraceuticals to promote health, as preservatives to delay peroxidation of foods, and as flavoring for packed foods.
Sujet(s)
Antioxydants/usage thérapeutique , Apocynaceae/composition chimique , Ficus/composition chimique , Flavonoïdes/analyse , Grewia/composition chimiqueRÉSUMÉ
Ficus religiosa L. is one of the most valued cultivated ornamental fig trees. Native to Asia, it is known as Bodhi tree. Since fig trees are involved in a highly species-specific pollination mutualism with agaonid wasps, exotic fig species usually fail to produce seeds due to the lack of the specific pollinating wasps. Since 2005 we have been observing plantlets of F. religiosa growing on buildings in Rio de Janeiro city, Brazil, showing that this species was hosting pollinating wasps and, consequently, undertaking sexual reproduction outside its native range. We observed that Platyscapa quadraticeps Mayr, 1885, the original pollinator in Asia, was occurring in figs of F. religiosa in Brazil. Considering that several non-pollinating fig wasp species are associated with F. religiosa in its native areas, novel occurrences of wasps may be reported in the future in Brazil. The presence of the native pollinator provides conditions for this exotic tree to become naturalized and/or invasive in Brazil, potentially causing considerable damage to buildings and urban trees.
Ficus religiosa L. é uma das figueiras ornamentais mais valorizadas. Nativa da Ásia, é conhecida popularmente como figueira-dos-pagodes. Como as figueiras apresentam um mutualismo especializado com vespas Agaonidae as espécies exóticas geralmente não se reproduzem sexualmente devido a falta dos polinizadores específicos. Desde 2005 nós observamos plantas jovens de F. religiosa crescendo sobre construções na cidade do Rio de Janeiro, Brasil, indicando que esta espécie está interagindo com vespas polinizadoras e, consequentemente, se reproduzindo sexualmente fora de sua área nativa. Observamos que Platyscapa quadraticeps Mayr, 1885, o polinizador original na Ásia, estava ocorrendo em figos dessa espécie no Brasil. Considerando que várias espécies não-polinizadoras de vespas de figo são associadas a F. religiosa em suas áreas nativas, novas ocorrências de vespas podem ser relatadas no futuro no Brasil. A presença do polinizador nativo provem condições para que esta espécie exótica se naturalize e/ou se torne invasiva no Brasil, com grande potencial de causar danos em prédios e árvores urbanos.
Sujet(s)
Symbiose , Guêpes , Ficus , PollinisationRÉSUMÉ
Background: Renal disorders including renal failure are common complications which affect the whole body system. The treatment methods in modern medicine seem to be inadequate in a large number of cases. Regarding the increasing interest of the community towards traditional and complementary medicine, the potential of Persian medicine [PM] in management of renal diseases, could be put to test in research programs in order to find possible effective treatment. Objective: The aim of this study is to introduce materia medica which are commonly used in PM for the purpose of protection and functional empowerment of the kidneys against destructive factors
Methods: This research is a literature-based study on effective material medica used in the treatment of two significant renal disorders; renal weakness and renal atrophy [Hozal]. Six pharmaceutical references of Persian medicine from different historical periods were searched by certain keywords related to renal weakness and renal atrophy
Results: Fourteen materia medica related to the prevention or treatment of renal weakness and fifteen for renal atrophy were found. Poppy [Papaver somniferum]. Amber and shellac are the most repeated medicines in the treatment of renal weakness; whereas fig [ficus carica], banana [musa sapientum] and hazelnut [corynus avelana] are mostly_repeated for either prevention or treatment of renal atrophy
Conclusion: Medicinal plants represented by Persian medicine references could be a basis for experimental and clinical researches for drug development in the field of prevention or even treatment of acute or chronic renal failure
Sujet(s)
Maladies du rein , Plantes médicinales , Phytothérapie , Extraits de plantes , Défaillance rénale chronique/prévention et contrôle , Papaver , Ambre , Ficus , Musa , CorylusRÉSUMÉ
Ficus carica L. (common fig), one of the first plants cultivated by humans, originated in the Mediterranean basin and currently grows worldwide, including southwest Asia and South Korea. It has been used as a traditional medicine for treatment of metabolic, cardiovascular, and respiratory diseases as well as hemorrhoids and skin infections. Its pharmacological properties have recently been studied in detail, but research on the anti-cancer effect of its latex has been only been studied on a limited basis on several cell lines, such prostate cancer, breast cancer, and leukemia. In this study, we investigated the anti-cancer activity of the latex of Ficus carica L.and its underlying mechanism in FaDu human hypopharynx squamous carcinoma cells. (See Ed. note above) We confirmed through SDS-PAGE analysis and gelatinolytic activity analysis that the latex of Ficus carica contains cysteine protease ficin. Our data showed that the latex inhibited cell growth in a dose-dependent manner. In addition, the latex treatment markedly induced apoptosis in FaDu cells as determined by FACS analysis, elevated expression level of cleaved caspase-9, -3 and PARP (poly (ADP-ribose) polymerase), and. increased the expression of Bax (pro-apoptotic factor) while decreasing the expression of Bcl-2 (anti-apoptotic factor). Taken together, these results suggested that latex containing the ficin inhibited cell growth and induced apoptosis by caspase and the Bcl-2 family signaling pathway in FaDu human hypopharynx squamous carcinoma cells. These findings point to the potential of latex of Ficus carica to provide a novel chemotherapeutic drug due to its growth inhibition effects and induction of apoptosis in human oral cancer cells.
Sujet(s)
Humains , Humains , Apoptose , Asie , Tumeurs du sein , Carcinome épidermoïde , Carica , Caspase-9 , Lignée cellulaire , Cysteine proteases , Électrophorèse sur gel de polyacrylamide , Ficine , Ficus , Hémorroïdes , Partie laryngée du pharynx , Corée , Latex , Leucémies , Médecine traditionnelle , Tumeurs de la bouche , Tumeurs de la prostate , PeauRÉSUMÉ
Phytophotodermatitis is a condition caused by sequential exposure to photosensitizing substances present in plants followed by ultraviolet light. Several plants (e.g., limes, celery, fig, and wild parsnip) contain furocoumarin compounds (psoralens). It is important for dermatologists to be aware of phytophotodermatitis because it may be misdiagnosed as cellulitis, tinea, or allergic contact dermatitis. We present five patients with a sharply defined erythematous swollen patch with bullae on both feet. They described soaking their feet in a fig leaves decoction to treat their underlying dermatologic diseases. Within 24 hours, all patients had a burning sensation in their feet, and erythema and edema had developed on the feet dorsa with exception of the portion of the skin covered by the sandals. Histopathologic examinations revealed sub-epithelial blisters with intensive epidermal necrosis. Phytophotodermatitis was ultimately diagnosed and, after several days, the patients' skin lesions began to recover upon treatment with systemic and topical corticosteroids. Unfortunately, since there are no studies providing sufficient evidence on the benefits of fig leaves, they should be used with caution.
Sujet(s)
Humains , Hormones corticosurrénaliennes , Apium , Cloque , Brûlures , Cellulite sous-cutanée , Citrus aurantiifolia , Eczéma de contact allergique , Oedème , Érythème , Ficus , Pied , Nécrose , Photodermatoses , Sensation , Peau , Teigne , Rayons ultravioletsRÉSUMÉ
Phytophotodermatitis is a condition caused by sequential exposure to photosensitizing substances present in plants followed by ultraviolet light. Several plants (e.g., limes, celery, fig, and wild parsnip) contain furocoumarin compounds (psoralens). It is important for dermatologists to be aware of phytophotodermatitis because it may be misdiagnosed as cellulitis, tinea, or allergic contact dermatitis. We present five patients with a sharply defined erythematous swollen patch with bullae on both feet. They described soaking their feet in a fig leaves decoction to treat their underlying dermatologic diseases. Within 24 hours, all patients had a burning sensation in their feet, and erythema and edema had developed on the feet dorsa with exception of the portion of the skin covered by the sandals. Histopathologic examinations revealed sub-epithelial blisters with intensive epidermal necrosis. Phytophotodermatitis was ultimately diagnosed and, after several days, the patients' skin lesions began to recover upon treatment with systemic and topical corticosteroids. Unfortunately, since there are no studies providing sufficient evidence on the benefits of fig leaves, they should be used with caution.
Sujet(s)
Humains , Hormones corticosurrénaliennes , Apium , Cloque , Brûlures , Cellulite sous-cutanée , Citrus aurantiifolia , Eczéma de contact allergique , Oedème , Érythème , Ficus , Pied , Nécrose , Photodermatoses , Sensation , Peau , Teigne , Rayons ultravioletsRÉSUMÉ
Abstract:It has been assumed that Tetrapus female wasps (Agaonidae s.s.), the pollinators of the figs of the New World, section Pharmacosycea, are mainly characterized by the presence of one mandibular appendange only, and that it is the most ancestral clade of extant Agaonidae s.s., and the males are tetrapodous. The main objective of this work was to study five Tetrapus morphotypes, whose females have two mandibular appendages and the males are hexapodous, their fig host association and phylogenetic position to the family Agaonidae. The question of which group of fig pollinating wasps and associated figs are the sister to the rest of the pollinating agaonids, and figs respectively remain open. I report a group of New World extant Tetrapus morphotypes (Agaonidae: Agaoninae) provisionally assigned to Hexapus subg. nov. in preparation. Currently, Tetrapus appears as the sister taxon to all other fig pollinating taxa. Howeveer, morphologically, ecologically, geographically and historically Hexapus seems to be the ancestral clade of the extant Agaoninae. Hexapus morphotypes develop in fig species of subsection Petenenses (section Pharmacosycea). In the known extant Tetrapus, the females have one mandibular appendage and the males have reduced one or two short-lobe atrophied non-functional midlegs (tetrapodous). Hexapus females have two free mandibular appendages, and the males have five segmented functional mid-legs (hexapodous). Molecularly Hexapus seems to be the ancestral clade of extant Agaoninae; e.g., a Tetrapus sp. of Ficus crassivenosa was placed by other author as the ancestral clade of 101 wasp species, representing 19 worldwide Agaoninae genera, including four Tetrapus species. In Tetrapus sp. of Ficus crassivenosa, the female has two mandibular appendages and the male is hexapodous. The females of T. apopnus and T. delclosi, preserved in Early to mid-Miocene amber from the Dominican Republic, also have two mandibular appendages and the general morphology of extant Hexapus. I suggest that Hexapus stands up as a living fossil and the sister clade of Tetrapus s.s. The presence of extant Hexapus; as well as extant Tetrapus and their fig host species, especially in South America supports a Southern Gondwanaland origin for both of them, but not a trans-Pacific migrating connection with tropical America for section Pharmacosycea (the host of Tetrapus and Hexapus), a long oceanic dispersal, high levels of stem extinction of Tetrapus or Atlantic land connections, as proposed by other authors. However, lastly it has been assumed that figs and their pollinators arose simultaneously in Eurasia during early Tertiary and spread southwards from it. Most of the morphological and molecular studies of the fig biology of Agaoninae and Ficus, did not include Hexapus morphotypes and their fig hosts, and assumed that Tetrapus is the most ancestral clade of the extant fig pollinating wasps. Rev. Biol. Trop. 64 (4): 1721-1735. Epub 2016 December 01.
Resumen:Se ha asumido que las hembras de las avispas Tetrapus (Agaonidae s.s.), las polinizadoras de los higos de la sección Pharmacosycea del Nuevo Mundo, se caracterizan principalmente por la presencia de un solo apéndice mandibular, que es el clado más ancestral de los Agaonidae s.s. existentes y los machos son tetrápodos. El principal objetivo de este trabajo fue estudiar algunos morfotipos de avispas Tetrapus, cuyas hembras poseen dos apéndices mandibulares y los machos tres pares de patas funcionales (hexápodos); su asociación con sus higos hospederos y la posición filogenética en la familia Agaonidae. Cuestiono cuál grupo de avispas polinizadoras de los higos, constituye el grupo hermano de otras avispas polinizadoras de los higos (Agaonidae). Informo sobre un grupo de avispas (Tetrapus: Agaoninae) existentes, provisionalmente asignado a Hexapus subg. nov. en preparación. Morfológica, ecológica, geográfica e históricamente, Hexapus parece ser el clado ancestral de los polinizadores de higos Agaoninae existentes. Los morfotipos de Hexapus se desarrollan en especies de higos de la subsección Petenenses (sección Pharmacosycea). Las hembras de Tetrapus existentes tienen únicamente un apéndice mandibular y los machos tienen uno o dos lóbulos cortos, en lugar de las patas medias y por lo tanto no son funcionales (tetrápodos). Las hembras de Hexapus tienen dos apéndices mandibulares libres y los machos tienen patas medias funcionales con cinco segmentos (hexápodos). Molecularmente, Hexapus parece ser el clado ancestral de los Agaoninae existentes; por ejemplo, una especie de Tetrapus de Ficus crassivenosa fue colocado por otros autores como el clado ancestral de 101 especies de avispas que representan 19 géneros de Agaoninae mundiales, incluidas cuatro especies de Tetrapus. En Tetrapus sp. de Ficus crassivenosa, la hembra tiene dos apéndices mandibulares y el macho es hexápodo. Las hembras de T. apopnus y T. delclosi, descritas del Mioceno Temprano (Burdigaliense), conservadas en ámbar de la República Dominicana, también tienen dos apéndices mandibulares y la morfología general de Hexapus existente. El autor sugiere que Hexapus representa un fósil viviente y es un clado hermano de Tetrapus s.s. La presencia de Hexapus y Tetrapus actuales, y sus higos hospederos, especialmente en Suramérica, sugieren que tienen un origen en la Gondwana meridional del Cretácico. La presencia de Hexapus y Tetrapus en el Nuevo Mundo, no parece apoyar una conexión migratoria a través del Pacífico con América tropical, para los higos de la sección Pharmacosycea (los hospederos de Tetrapus y Hexapus), una amplia dispersión transoceánica, altos niveles de extinción básica de Tetrapus y conexiones terrestres a través del Atlántico, propuesto por otros autores. Sin embargo, últimamente se ha aceptado que los higos y sus polinizadores se originaron simultáneamente en Eurasia, durante el Terciario Primario y se diseminaron hacia el sur. La mayoría de estudios morfológicos y moleculares de biólogos de los Agaoninae y Ficus, no incluyeron morfotipos de Hexapus, o sus especies hospederas de Ficus y aceptadaron que Tetrapus es el clado más ancestral de las avispas polinizadoras de los higos existentes.
Sujet(s)
Animaux , Mâle , Femelle , Guêpes/anatomie et histologie , Ficus/physiologie , Pollinisation/physiologie , Phylogenèse , Pollen/anatomie et histologie , Spécificité d'espèce , Facteurs sexuels , Mandibule/anatomie et histologieRÉSUMÉ
The aim of this study was to determine the lifespan in making jam by higueron and carrot mixture, motivated by the lack of a number of species of tubers and vegetables, as evidenced by the low consumption of these foods and the lack of appropriate technology for processing the same. The two raw materials are high in vitamins and minerals, higueron suitable for their characteristics and chemical composition is considered a fruit-vegetable, it is possible it industrialization similar to those made with fruits, like the carrot is a food products excellent from the nutritional point of view, contributing to improve the quality of the jam. The treatments were kept under ambient conditions for 40 days when pH were recorded - soluble solids (° Brix) - Heartburn - Microbiological and organoleptic analysis in order to identify the best experimental treatment tests. According to the results obtained and microbiological analysis establishes a lifetime about 6 months.
El objetivo de este estudio fue conocer el tiempo de vida útil en la elaboración de mermelada mediante una mezcla higuerón y zanahoria, motivado por el desconocimiento de un sinnúmero de especies de tubérculos y hortalizas, evidenciado por el bajo consumo de estos alimentos y la carencia de tecnología adecuada para el procesamiento del mismo y aprovechamiento de sus nutrientes. Las dos materias primas de alto contenido de vitaminas y minerales; el higuerón se considera como fruta-hortaliza por sus características y composición química, siendo posible la industrialización en productos semejantes a los elaborados con las frutas. La zanahoria es un alimento excelente desde el punto de vista nutricional por lo que aporta al mejoramiento de la calidad de la mermelada. Los tratamientos se mantuvieron bajo condiciones ambientales durante 40 días en los que se registró pH - sólidos solubles (°Brix) - acidez - análisis microbiológicos y pruebas organolépticas con la finalidad de identificar el mejor tratamiento experimental. De acuerdo a los resultados obtenidos y al análisis microbiológico se establece un tiempo de vida útil alrededor de 6 meses.
Sujet(s)
Aliments , Daucus carota , Ficus , Confiture de Fruits , Manipulation des aliments , Légumes , Stockage des alimentsRÉSUMÉ
Resumo O farmacêutico Theodoro Peckolt é uma das mais importantes figuras da história da química de produtos naturais brasileira. Como outros farmacêuticos do século XIX que atuavam no Brasil, desenvolveu formulações que comercializava em sua farmácia, localizada no Rio de Janeiro, e que tiveram grande prestígio junto à população e à classe médica. O texto apresenta a relação entre a doença identificada inicialmente como opilação e a terapêutica utilizada no século XIX, destacando uma das formulações da Farmácia Peckolt – “Pós de doliarina e ferro”. O produto tem sua origem no látex da espécie Ficus gomelleira(figueira-branca ou gameleira). O artigo tem entre seus objetivos revelar a composição química, feita por métodos modernos de análise do látex deFicus gomelleira.
Abstract The pharmacist Theodoro Peckolt was one of the most important figures in the history of the chemistry of natural Brazilian products. Like other nineteenth-century pharmacists in Brazil, he developed formulations and sold them at his pharmacy in Rio de Janeiro, and these enjoyed great prestige in the eyes both of the public and the medical community. The article discusses the relation between the illness originally called “opilação” (ancylostomiasis, or hookworm) and nineteenth-century treatment. It focuses especially on Peckolt Pharmacy’s “Doliarina and iron powder,” a formulation extracted from the Ficus gomelleira rubber plant. One of the article’s goals is to use modern methods to analyze Ficus gomelleira and identify the chemical composition of the drug.
Sujet(s)
Humains , Animaux , Histoire du 19ème siècle , Pharmacies/histoire , Ficus/composition chimique , Infections à ankylostomes/histoire , Antihelminthiques antinématodes/histoire , Pharmaciens/histoire , Brésil , Infections à ankylostomes/traitement médicamenteux , Fer/histoire , Fer/usage thérapeutique , Antihelminthiques antinématodes/usage thérapeutique , Antihelminthiques antinématodes/composition chimiqueRÉSUMÉ
Abstract We analyzed the phenology of Ficus adhatodifolia Schott ex Spreng. (23 fig tree) and F. eximia Schott (12 fig tree) for 74 months in a remnant of seasonal semi-deciduous forest (23°27’S and 51°15’W), Southern Brazil and discussed their importance to frugivorous. Leaf drop, leaf flush, syconia production and dispersal were recorded. These phenophases occurred year-round, but seasonal peaks were recorded in both leaf phenophases for F. eximia and leaf flushing for F. adhatodifolia. Climatic variables analyzed were positively correlated with reproductive phenophases of F. adhatodifolia and negatively correlated with the vegetative phenophases of F. eximia. In despite of environmental seasonality, little seasonality in the phenology of two species was observed, especially in the reproductive phenology. Both species were important to frugivorous, but F. adhatodifolia can play a relevant role in the remnant.
Resumo Foi analisada a fenologia de Ficus adhatodifolia Schott ex Spreng. (23 indivíduos) e F. eximia Schott (12 indivíduos), por 74 meses em um remanescente de floresta estacional semidecidual (23°27’S e 51°15’W, centro de visitantes) no sul do Brasil e discutido sua importância para os frugívoros. Foram registradas as fenofases: queda e brotamento de folhas, produção e dispersão de siconios. Estas fenofases ocorreram ao longo do ano, porém picos sazonais foram registrados em abscisão e brotamento foliares para F. eximia e brotamento foliar para F. adhatodifolia. As variáveis climáticas analisadas foram positivamente correlacionadas com as fenofases reprodutivas de F. adhatodifolia e negativamente correlacionadas com as fenofases vegetativas de F. eximia. Apesar da sazonalidade ambiental, foi observada baixa variação sazonal na fenologia das duas espécies, especialmente na fenologia reprodutiva. Ambas as espécies foram consideradas importantes para os frugivoros, porém F. adhatodifolia pode desempenhar papel de destaque no fragmento florestal.
Sujet(s)
Ficus/croissance et développement , Arbres/croissance et développement , Brésil , Forêts , Feuilles de plante/croissance et développement , Reproduction , Saisons , Spécificité d'espèceRÉSUMÉ
An understanding of the complex ecological interaction between fig wasps and their host plants in Amazonia requires previous knowledge of their distribution and diversity. The objective of this study was to describe the composition and structure of the wasp community associated with four species of Ficus in the municipal area of Manaus, Amazonas, Brazil. A total of 600 syconia from four species were collected. The study species were: Ficus obtusifolia Kunth; Ficus citrifolia Mill; F. americana subspecies guianensis Desv. form mathewsii; and F. americana subspecies guianensis Desv. form parkeriana. Statistical analyses were used to examine the relationship between fig wasp diversity and syconium diameter, and the effect of non-pollinating wasps on numbers of pollinators and seeds. Forty three species of fig wasp were identified, distributed across seven genera (Pegoscapus, Idarnes, Aepocerus, Physothorax, Anidarnes, Heterandrium , Eurytoma). Idarnes (carme group) was the wasps genus non-pollinator with greatest number of individuals with the greatest number of infested syconia (7409 wasps in 376 syconia). Analysing non-pollinating wasp diversity in relation to fig diameter, a significant difference was observed between the four fig species. Ficus citrifolia and F. americana subspecies guianensis form mathewsii had the smallest diameter but the greatest diversity of fig wasp. Ficus obtusifolia was the only species in which the non-pollinating wasps had a significant negative effect on the number of Pegoscapus sp. and on seed production.
A compreensão sobre as complexas interações ecológicas envolvendo vespas-de-figo e suas plantas hospedeiras na Amazônia depende do conhecimento prévio sobre a distribuição e diversidade dessas vespas. O objetivo deste estudo foi descrever a composição e estrutura da comunidade de vespas associadas a quatro espécies de Ficus no município de Manaus, Amazonas. Foram coletados e analisados 600 sicônios de quatro espécies: Ficus obtusifolia Kunth; Ficus citrifolia Mill; F. americana subspecies guianensis Desv. forma mathewsii; and F. americana subspecies guianensis Desv. forma parkeriana. Análises estatísticas foram usadas para analisar a relação entre a diversidade de vespas-de-figo e o diâmetro do sicônio, e o efeito das vespas não-polnizadoras em relação ao número de polinizadoras e sementes. Foram encontradas 43 espécies de vespas-de-figo distribuídas em sete gêneros (Pegoscapus, Idarnes, Aepocerus, Physothorax, Anidarnes, Heterandrium , Eurytoma). Idarnes grupo carme foi o gênero de vespas não-polinizadora com maior numero de individuos com maior numero de sicônios infestado (7.409 vespas em 376 sicônios). Ao analisar a diversidade de vespas não-polinizadoras em relação ao diâmetro do figo observou-se diferença significativa entre as quatro espécies de figueiras. Ficus citrifolia e F. americana, subespécie guianensis, forma mathewsii apresentaram o diâmetro menor, porém uma maior diversidade de vespas-de-figo. Ficus obtusifolia foi a única espécie em que as vespas não-polinizadoras exerceram efeito negativo significativo tanto no número de Pegoscapus sp. quanto na produção de sementes.
Sujet(s)
Animaux , Biodiversité , Ficus , Guêpes , Écosystème Amazonien/analyseRÉSUMÉ
O gênero Cryptococcus caracteriza-se por ser uma levedura responsável por infecção sistêmica, causada pelas espécies Cryptococcus neoformans e Cryptococcus gattii. O fungo é encontrado em substratos de origem animal e vegetal, e a infecção ocorre com a inalação de basidiósporos ou leveduras desidratadas infectantes presentes no ambiente. O presente trabalho teve por objetivo pesquisar a existência de microfocos de Cryptococcussp.em amostras ambientais da cidade de Araçatuba, São Paulo, com a finalidade de minimizar os riscos de contaminação do homem e dos animais, buscando o conhecimento da ecoepidemiologia do Cryptococcus. Foram colhidas 50 amostras oriundas de ocos e troncos de árvores (Cassiasp., Ficussp., Caesalpinea peltophorides) de 10 locais representativos do perímetro urbano, as quais foram encaminhadas ao Laboratório de Bacteriologia e Micologia da Faculdade de Medicina Veterinária de Araçatuba-Unesp, onde foram processadas e semeadas em placas de Petri contendo ágar semente de Níger e Sabouraud dextrose com clorafenicol e incubadas à temperatura de 30ºC, por um período não inferior a cinco dias. Posteriormente, foram submetidas às provas bioquímicas: produção de urease, termotolerância a 37ºC e quimiotipagem em ágar CGB (L-canavanina-glicina-azul de bromotimol). A análise dos resultados revelaram que 17 (34%) dos cultivos foram positivos para o gênero Cryptococcus, sendo nove (18%) para Cryptococcus gattiie oito (16%) para Cryptococcus neoformans. Outras leveduras correlacionadas, como Rhodotorula sp. e Candida sp., também foram isoladas. Conclui-se que os basidiósporos de Cryptococcusencontram-se dispersos na natureza, constituindo microfocos ambientais, não vinculados necessariamente a um único hospedeiro.
Cryptococcosis is an opportunistic fungal infection caused by Cryptococcusyeasts, especially C. neoformans and Cryptococcus gattii. The fungus is found in substrates of animal and vegetable origin, and infection occurs through inhalation and seedlings present in the environment. The present study aimed to investigate the existence of microfocus Cryptococcus sp. from the environmental samples of Araçatuba city, São Paulo, featuring new niches, by decoupling the direct relationship between fungus and host in order to minimize the risk of contamination of man and animals, understanding the ecoepidemiology of Cryptococcus. Fifty samples from hollows and tree trunks were harvested (Cassia sp., Ficus sp., Caesalpinea peltophorides) from ten representatives in the urban perimeter. The samples were immediately sent to the Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine Araçatuba - Unesp where they were processed and plated on Petri dishes containing agar seed Niger and Sabouraud dextrose agar with chloramphenicol, incubated at 30ºC for a period of no less than 5 days. Afterwards they were subimitted to biochemical tests: urease production, thermotolerance at 37°C and quimiotipagem in CGB agar (L- Canavanine-Glycine-Bromothymol blue). The results showed that 17 (34%) cultures were positive for Cryptococcus, 9 (18%) for Cryptococcus gattii and 8 (16%) for Cryptococcus neoformans. Other yeast correlated as Rhodotorula sp. and Candida sp. were isolated. We conclude that the infectious propagules of Cryptococcus are dispersed in nature and constitute an environmental microfocus, not necessarily being bound to a single host.
Sujet(s)
Cryptococcus neoformans , Champignons , Infections , Cassia , Entomophthorales , Ficus , LevuresRÉSUMÉ
An antibacterial benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, isolated from Ficus foveolata stems was used as a standard marker for establishment of quantitative HPLC analysis for the stem extracts of F. foveolata . The method utilized a TSK-gel ODS-80Ts column (5 microm, 4.6 x 250 mm) with the mixture of methanol and 5% acetic acid in water (24:76, v/v) as the mobile phase at a flow rate of 1 mL/min, and quantitative detection at 289 nm. The parameters i.e. linearity, intraday and interday precision, accuracy, specificity and sensitivity of the method were evaluated for method validation. The recoveries of the method were 99.5 - 103.6% and good linearity (R2 > or = 0.9999) was obtained. A high degree of specificity, sensitivity as well as repeatability and reproducibility (RSD less than 2 and 5%, respectively) were also achieved. Chloroform was served as the most suitable solvent for extraction of 2,6-dimethoxy-1,4-benzoquinone. The optimised sample preparation and HPLC method can be practically used in the routine quality control process of F. foveolata stem extracts.
Sujet(s)
Acide acétique , Chloroforme , Chromatographie en phase liquide à haute performance , Ficus , Méthanol , Contrôle de qualité , Sensibilité et spécificité , EauRÉSUMÉ
Farmacopea Tacana. Se realizó el estudio de 2 especies vegetales antiparasitarias de la Farmacopea Tacana: Galipea longiflora (Evanta) y Ficus spp. (Ojé). El estudio de la Evanta comprende a su vez: el estudio de plantines de diferentes tamaños, este se centra en el análisis de variaciones de la mezcla de alcaloides quinolínicos y la actividad antiparasitaria frente a la variación del tamaño del plantin; y la validación del uso tradicional como antiparasitario, mediante un estudio de campo, que compara la efectividad de la decocción de alcaloides de Evanta en jugo de naranja, frente a un fármaco de actividad reconocida (Mebendazol). En el caso del Ojé, se realizaron estudios comparativos de las proteasas contenidas en su látex, colecta do en diferentes zonas [Iquitos-Perú; Provincia Sud Yungas y Provincia Abel Iturralde, La Paz-Bolivia] Materiales y métodos. En el estudio de plantines se obtuvieron los alcaloides totales de plantin (PAT), los cuales fueron analizados por cromatografía de gases - masas (GC/MS) y evaluados frente a promastigotes de Leishmania. Para la validación de Evanta como antiparasitario, se realizó un examen coproparasitológico seriado a niños de la escuela Charcas II, aplicando el método de Ritchie para la evaluación microscópica. En el estudio del Ojé, se emplearon métodos de cuantificación de proteínas (Biuret), determinación de actividad proteolítica sobre azocaseína (Cristen-Marshall) y leche (Balls y Hoover), además de caracterización por SDS-PAGE. Resultados Se identificaron hasta 14 alcaloides quinolínicos, 5 de ellos comunes entre adultos y plantines, otros aparecen cuando el plantin tiene mayor edad. Se obtuvo una mejor actividad biológica para los plantines con respecto al árbol adulto. La validación de Evanta como antiparasitario, en general, muestra un control en los niveles de parasitosis y la disminución de parásitos por muestra. A nivel de tratamientos tanto la decocción de Evanta como el Mebendazol presentan alta tasa de curación para helmintos, en protozoarios los niveles de eliminación son mínimos. La administración de placebo (2013) confirma la eficacia de los tratamientos al existir un incremento en la prevalencia de parásitos; reapareciendo parásitos que se habían eliminado y otros que no se reportaron al inicio del estudio. El estudio del látex de Ojé muestra concentraciones de proteínas, actividad proteolítica y especies proteínicas por SDS-PAGE variables entre zonas de colecta. Además de la conservación. (AU)
Sujet(s)
Humains , Ficus , Rutaceae , Antiparasitaires , Plantes médicinales , Bolivie , Médecine traditionnelleRÉSUMÉ
Avaliou-se a evapotranspiração (ETc) e a eficiência do uso da água (EUA) pela figueira 'Roxo de Valinhos' submetida à irrigação e cobertura morta (bagacilho de cana-de-açúcar triturado) no primeiro ciclo produtivo, nas condições edafoclimáticas de Botucatu, São Paulo. Empregou-se o método do balanço hídrico do solo e para a obtenção da evapotranspiração de referência foi utilizado o método Penman Montheit FAO 56. Para a avaliação dos coeficientes de cultivo (kc) adotou-se a seguinte distribuição fenológica: fase 1 - entre o transplantio e 20% do desenvolvimento vegetativo (DV); ii) fase 2 - de 20 a 80% DV; e iii) fase 3 frutificação. Observam-se ETc acumulados de 409,4 e 465,8 mm em 254 dias após o transplantio (DAT) e médias de 1,47 e 1,67 mm dia-1, com e sem cobertura morta (CC e SC). Os coeficientes de cultivo (kc) médios foram de 0,16; 0,43 e 0,49 para SC e de 0,18; 0,44 e 0,50 para CC, entre as fases 1 e 3, respectivamente. Os valores de EUA diminuem com o aumento do volume de água recebido e variaram entre 1,65 e 3,32 kg de figos verdes por m3 de água irrigada para SC e CC.
Evaluated the evapotranspiration (ETc) and the efficiency of water use (USA) by the fig tree 'Purple Valinhos' submitted to irrigation and mulching (bagacilho of sugar cane crushed) in the first production cycle, at conditions of Botucatu , St. Paul. We used the method of soil water balance and to obtain the reference evapotranspiration method was used Montheit FAO Penman 56. For the assessment of crop coefficients (kc) we adopted the following phenological distribution: phase 1 - between transplanting and 20% of the vegetative (DV), ii) phase 2 - 20 to 80% DV, and iii) phase 3 - fruiting. Observe the cumulative ETc 409.4 and 465.8 mm in 254 days after transplanting (DAT) and averages of 1.47 and 1.67 mm day-1, with and without mulching (CC and SC). The crop coefficients (kc) mediums were 0.16, 0.43 and 0.49 for SC and 0.18, 0.44 and 0.50 for CC, in phases 1 and 3, respectively. The EUA values decrease with increasing the volume of water received and ranged between 1.65 and 3.32 kg of green figs per m3 of water for irrigated SC and CC.