RÉSUMÉ
Abstract A cross-sectional study was carried out on a sample of 379 horses to determine the seroprevalence of Neospora spp. in Jordan using the indirect fluorescent antibody test. Five variables, namely locality (n=10), climatic zone (n=4), age group (n=3), gender, and breed were tested as risk factors for Neospora-immunoglobulin (Ig)G seropositivity at four cutoff titers (1:50, 1:200, 1:400, and 1:800) using univariate and multivariate logistic regression analyses. A total of 122 (32%; 95% CI: 28, 37) sera samples had anti-Neospora-IgG at a cutoff titer of 1:50. Increased Neospora-IgG seropositivity was found in horses in three localities (Madaba, Zarka, and Petra) and was associated with the following variables: cool temperate climate; age >14 years; and female gender. Seropositivity was found among horses from Madaba at all cutoff titers, Zarka at titers >1:200, and Petra at titers <1:200. Cool temperate climate was associated with titers <1:400. Horses aged >14 years were found to be associated with seropositivity at titers ≥1:200. Female gender was associated with high seropositivity at >1:800.
Resumo Um estudo transversal foi realizado, na Jordânia, em uma amostra de 379 cavalos, para determinar a soroprevalência de Neospora spp., usando-se o teste de anticorpos fluorescentes indiretos. Cinco variáveis: localidade (n=10), zona climática (n=4), grupo etário (n=3), sexo e raça, foram testadas como fatores de risco para soropositividade para Neospora-imunoglobulina (Ig)G, considerando-se quatro pontos de corte (1:50, 1:200, 1:400 e 1:800) por meio de análises de regressão logística univariada e multivariada. Um total de 122 (32%; 95% CI: 28, 37) amostras de soros apresentaram anti-Neospora-IgG, utilizando-se como ponto de corte o título de 1:50. Cavalos de três localidades apresentaram aumento da soropositividade para Neospora-IgG (Madaba, Zarka e Petra) o que foi associado às seguintes variáveis: clima temperado fresco; idade >14 anos; e sexo feminino. Os cavalos de Madaba apresentaram soropositividade em todos os títulos utilizados como ponto de corte; os cavalos de Zarka em títulos >1:200; e os cavalos de Petra em títulos <1:200. O clima temperado fresco foi associado aos títulos <1:400. Cavalos com idade >14 anos estiveram associados à soropositividade nos títulos ≥1:200. O sexo feminino esteve associado à alta soropositividade nos títulos >1:800.
Sujet(s)
Animaux , Mâle , Femelle , Anticorps antiprotozoaires/sang , Coccidiose/médecine vétérinaire , Neospora/immunologie , Maladies des chevaux/épidémiologie , Études séroépidémiologiques , Facteurs sexuels , Études transversales , Facteurs de risque , Facteurs âges , Coccidiose/diagnostic , Coccidiose/épidémiologie , Maladies des chevaux/diagnostic , Equus caballus , Jordanie/épidémiologieRÉSUMÉ
Abstract Objective: The aim of this study was to evaluate the incidence of postoperative nausea and vomiting (PONV) after fast-track cardiac anesthesia (FTCA) in the first 24-48 hours in the cardiac intensive care unit (CICU) after open-heart surgery, risk factors for PONV and its influence on CICU length of stay. Methods: A prospective observational study from January 1, 2013 to the end of December 2015 was performed in the CICU of a university hospital in the north of Jordan and Queen Alia Heart Institute, Amman, Jordan. Three hundred consecutive patients undergoing fast-track cardiac anesthesia in elective cardiac surgery were enrolled in the study. Nausea and vomiting were assessed after tracheal extubation, which was performed within 6-10 hours after surgery and during the first 24-48 hours in the CICU. Metoclopramide 10 mg intravenously was used as the initial antiemetic drug, but ondansetron 4 mg intravenously was also used as second line of management. Results: Nausea was reported in 46 (15.3%) patients, and vomiting in 31 (10.3%). Among females, 38 (33.9%) patients developed nausea and 20 (17.9%) developed vomiting. Among males, 8 (4.3%) patients developed nausea and 11 (5.9%) developed vomiting. Conclusion: PONV are relatively low after FTCA and the prophylactic administration of antiemetic drug before anesthesia or after extubation is not necessary.
Sujet(s)
Humains , Mâle , Femelle , Adulte , Adulte d'âge moyen , Sujet âgé , Sujet âgé de 80 ans ou plus , Jeune adulte , Vomissements et nausées postopératoires/étiologie , Vomissements et nausées postopératoires/épidémiologie , Procédures d'anesthésie cardiaque/effets indésirables , Procédures de chirurgie cardiaque , Facteurs temps , Incidence , Études prospectives , Facteurs de risque , Répartition par sexe , Vomissements et nausées postopératoires/traitement médicamenteux , Unités de soins intensifs/statistiques et données numériques , Jordanie/épidémiologie , Durée du séjour , Antiémétiques/usage thérapeutiqueRÉSUMÉ
This study aimed to determine the seroprevalence and to identify risk factors associated with Neospora spp. infection in horses in Jordan. Management related data were collected from each farm and individual horses. Sera from 227 horses from 5 of 6 climatic regions in Jordan were analyzed for the presence of antibodies to Neospora spp. by ELISA kit. The study was performed during spring of 2010. The association between seropositivity and risk factors was analyzed. A total of 7 (3%) of 227 sera had antibodies for Neospora spp. There was a significant regional difference (P=0.018) between the 5 climatic regions. Positive cases were located in Amman and Irbid, while the other regions (Zarqa, Jordan Valley, and Wadi Mousa) had zero prevalence. The use of anthelmintics at least once a year resulted in a significant reduction of the seroprevalence to Neospora spp. (1.6% vs 9.8%). However, this might be a phenomenon by chance and a better hygiene since owners can invest in anthelmintics. Other risk factors such as age, gender, breed, usage, body condition score, grazing, presence of other animals mixed with the horses in the same property, and a history of previous diseases were not significantly associated with the seroprevalence to Neospora spp. infection. This is the first study to report on the presence of Neospora seropositive horses in Jordan. Further studies are warranted to better understand the role of certain risk factors in the transmission of Neospora spp. among horse population and to determine which Neospora spp. are responsible for the infection.
Sujet(s)
Animaux , Femelle , Mâle , Anticorps antiprotozoaires/sang , Maladies asymptomatiques/épidémiologie , Coccidiose/sang , Maladies des chevaux/sang , Equus caballus , Jordanie/épidémiologie , Neospora/immunologie , Études séroépidémiologiquesRÉSUMÉ
The bacterium Simkania negevensis is a germ associated with respiratory diseases. This study aims at estimating the prevalence of Simkania in the Jordanian population. Serum samples from 664 Jordanian males and females, aged 2 to 86 years were collected. IgG and IgM Simkania-specific antibodies were detected using an indirect immunofluorescence test. Seropositivity titers for IgG and IgM were defined as 1:8 and 1:10, respectively. The overall prevalence of IgG antibody in all examined Jordanian nationals was 58.4%. IgG seropositivity was low in children under the age of 10 years (34.2%), and increased rapidly with age and ranged between 49.4% and 72%. Simkania-specific IgM was detected in 24.8% of subjects. IgM prevalence in children under 10 years was lowest (10.5%) and increased in older ages and remained above 20%. Overall detection rates of both IgG and IgM were significantly higher in females than males (60.7% vs. 54.5% for IgG and 26.7% vs. 21.7% for IgM). These data indicate that Simkania infection is highly prevalent in Jordan. The high level of seropositivity is most likely maintained by re-infections or chronic infections. Our data may serve as a basis to elucidate the pathogenesis of Simkania in Jordan.
Sujet(s)
Adolescent , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Enfant , Enfant d'âge préscolaire , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Anticorps antibactériens/sang , Chlamydiales/immunologie , Infections bactériennes à Gram négatif/épidémiologie , Infections bactériennes à Gram négatif/microbiologie , Facteurs âges , Immunoglobuline G/sang , Immunoglobuline M/sang , Jordanie/épidémiologie , Études séroépidémiologiquesRÉSUMÉ
Aims: To determine the prevalence of Legionella spp. in domestic hot water systems and evaluate the molecular diversity among these Legionella spp. Isolates. Place and Duration of Study: Sample collection area was the city of Aqaba, Jordan, between May and December 2012. Sample analysis was done in Ben-Hayyan international laboratories, Aqaba city, and the molecular microbiology laboratories, Taibah University, Saudi Arabia. Methodology: Two hundred (200) water samples were collected randomly from hot water tanks of private apartments, and were tested for the occurrence of Legionella spp. using direct membrane filtration method followed by species identification using Gram stain, the API 20NE biochemical system and the Legionella species latex agglutination test. Genotype characterizations of the Legionella isolates was carried out using DNA extraction followed by RAPD-PCR amplification with OP-A3 primer and analysis of the resulting patterns. Results: Of the 200 samples, 17 (8.5%) were positive for the presence of Legionella spp. A total of 15 (88.2%) out the 17 positive samples were confirmed as Legionella pneumophila, 10 of them were of serogroup 1 and 5 isolates were of serogroup 2-14, the remaining two isolate were Legionella species other than L. pneumophila. RAPD-PCR analysis classified all 17 Legionella isolates into three groups. Serogroup 1 isolates were classified into group A, serogroup 2-14 isolates in group B and Legionella spp. isolates in group C. Group A was further sub-clustered into two subgroups, genotype A1 containing isolates collected from hot water tanks of a temperature set at 25-30°C and A2 containing isolates collected from hot water tanks of a temperature set at 55-80°C. Conclusion: This study showed the colonization of the plumbing systems of private houses by Legionella spp. and demonstrated that the temperature of the water tanks maybe one of the most important factors that affect the genotypic behavior of Legionella pneumophila.
Sujet(s)
Techniques de génotypage , Chauffage/méthodes , Logement , Humains , Jordanie/épidémiologie , Legionella pneumophila/analyse , Legionella pneumophila/génétique , Legionella pneumophila/isolement et purification , Réaction de polymérisation en chaîne/méthodes , Prévalence , Eau/microbiologieRÉSUMÉ
BACKGROUND: The human methionine synthase gene (MTR) is located on chromosome 1q43; it is of 105.24 kb and is made up of 33 exons. Methionine synthase is a cytoplasmic enzyme that requires methylcobalamin for activity and catalyzes the remethylation of homocysteine to methionine. In this reaction, the methyl group of 5-methyltetrahydrofolate is transferred to the enzyme bond cob(I) alamin to generate methylcobalamin, followed by the transfer of the methyl group to homocysteine to reform methionine. MATERIALS AND METHODS: The frequencies of the polymorphisms of MTR 2756A>G and MTR 2758C>G have been determined in this study in a sample of 491 individuals collected from all regions of Jordan and representing the Jordanian population. The different alleles and genotypes at the two polymorphic sites were identified using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. RESULTS: Showed that the percentages of the polymorphic alleles at the MTR 2756 position in the north, middle and south regions were 90.38, 92.65 and 83.69%, respectively, for the MTR 2756A allele, and were 9.61, 7.34 and 16.30%, respectively, for the MTR 2756G allele, with overall percentages in the whole Jordanian population of 90.73 and 9.27% for the MTR 2756A and MTR 2756G alleles, respectively. The percentages of the genotype MTR 2756AA were 82.90% in the northern region, 86.72% in the middle region and 71.73% in the southern region, and an overall percentage of MTR 2756AA in the whole Jordanian population was 83.50%. The frequencies of MTR 2756AG genotype in the northern, middle and southern regions were 14.95, 11.84 and 23.91%, respectively, with an overall percentage of 14.46% in the whole Jordanian population. The percentages of the genotype MTR 2756GG in the northern, middle and southern regions were 2.13, 1.42 and 4.34%, respectively, with an overall percentage of 2.04% in the whole Jordanian population. Only the wild type allele (C) of the MTR 2758C>G polymorphism was detected in this study. In addition, the association of MTR 2756A>G and MTR 2758C>G polymorphisms with the development of neural tube defects (NTDs) was examined using 17 cases of mothers from the northern part of Jordan, who gave birth to NTD affected children during the period of this study. Results showed no association between these two examined polymorphisms and the increase in maternal risk for giving birth to NTD children. CONCLUSION: results of this study recommend that examination should be done on larger populations to arrive at better conclusions. Also, more studies on gene–gene interaction should be done to examine the associations with NTDs.
Sujet(s)
Methyltransferases/génétique , Adulte , Femelle , Humains , Mâle , Jordanie/épidémiologie , Anomalies du tube neural/génétique , Groupes de population , Jeune adulteRÉSUMÉ
Objective: This study was designed to investigate the subjective ratings of dental anxiety levels among university students enrolled at Jordan University of Science and Technology. In addition, the present study aimed to explore the sources of dental anxiety and the impact of gender on the perceived dental anxiety and the correlation between field of study and dental anxiety level.Material and Methods: The Modified Corah Dental Anxiety Scale was used to measure dental anxiety among the study population. Six hundred subjects were recruited into the study from Jordanian undergraduate students from the faculties of Medicine, Engineering, and Dentistry. Results: Five hundred and thirty five complete questionnaires were returned, which accounts for a response rate of 89.2%. The totals of the mean anxiety scores were the following: Medical students, 13.58%; Engineering students, 13.27% and dental students, 11.22%. About 32% of the study population has scored 15 or more. Dental students had the lowest percentage of those who scored 15 or more. Surprisingly, the medical students were responsible for the highest percentage of those who scored 15or above. Although women demonstrated statistically higher total dental anxiety scores than men (p= 0.03), the difference between both genders was small and could be clinically insignificant. The students were anxious mostly about tooth drilling and local anesthetic injection. Conclusion: Lack of adequate dental health education may result in a high level of dental anxiety among nondentaluniversity students in Jordan. Further studies are required to identify the correlates of dental anxiety among university students.
Sujet(s)
Femelle , Humains , Mâle , Jeune adulte , Phobie des soins dentaires/épidémiologie , Étudiants/statistiques et données numériques , Phobie des soins dentaires/étiologie , Techniques dentaires à grande vitesse/effets indésirables , Détartrage dentaire/effets indésirables , Ingénierie/enseignement et éducation , Injections/effets indésirables , Jordanie/épidémiologie , Manifest anxiety scale , Facteurs sexuels , Enquêtes et questionnaires , Étudiant dentisterie/statistiques et données numériques , Étudiant médecine/statistiques et données numériques , Universités , Jeune adulteRÉSUMÉ
We investigated the seroprevalence and risk factors for Brucella seropositivity in cattle in Jordan. The sera from 671 cows were randomly collected from 62 herds. The antibodies against Brucella were detected using a Rose Bengal plate test and indirect ELISA. A structured questionnaire was used to collect information on the cattle herds' health and management. A multiple logistic regression model was constructed to identify the risk factors for Brucella seropositivity. The true prevalence of antibodies against Brucella in individual cows and cattle herds was 6.5% and 23%, respectively. The seroprevalence of brucellosis in cows older than 4 years of age was significantly higher than that in the younger cows. The seroprevalence of brucellosis in cows located in the Mafraq, Zarqa and Ma'an governorates was significantly higher than that of the other studied governorates. The multiple logistic regression model revealed that a larger herd size (odd ratio = 1.3; 95% CI: 1.1, 2.6) and mixed farming (OR = 2.0; 95% CI: 1.7, 3.7) were risk factors for cattle seropositivity to Brucella antigens. On the other hand, the use of disinfectants (OR = 1.9; 95% CI: 1.1, 2.1) and the presence of adequate veterinary services (OR = 1.6; 95% CI: 1.2, 3.2) were identified as protective factors.
Sujet(s)
Animaux , Bovins , Anticorps antibactériens/sang , Brucellose bovine/sang , Jordanie/épidémiologie , Facteurs de risque , Études séroépidémiologiquesRÉSUMÉ
OBJECTIVE: To assess the prevalence of dyspeptic symptoms in the general population of Jordan. METHODS: We selected families of final year students at the two medical schools in Jordan to represent the general population. The students were instructed to complete questionnaires for all their family members and all members of the close family above the age of 15 years living in the area. The questionnaire enquired about dyspeptic symptoms in the previous 12 months. Information about any chronic disease, medications used frequently, previous operations and cigarette smoking was obtained. RESULTS: Of 2254 completed questionnaires, dyspeptic symptoms were reported in 1354 (60.1%) during the previous 12 months. Heartburn was present in 1111 (49.3%), abdominal distension in 1224 (54.3%), recurrent abdominal pain with ulcer-like characters in 450 (20%), discomfort following meals in 316 (14%), symptoms related to fatty foods in 359 (15.9%) and symptoms related to ingestion of lactose-containing foods in 96 (4.3%). CONCLUSION: Dyspeptic symptoms are highly prevalent in Jordan. This may have an impact on the economy and health resources.
Sujet(s)
Adolescent , Adulte , Loi du khi-deux , Dyspepsie/épidémiologie , Femelle , Humains , Jordanie/épidémiologie , Mâle , Prévalence , Enquêtes et questionnaires , Facteurs de risqueRÉSUMÉ
To study the prevalence of Helicobacter pylori infection in dyspeptic Jordanian patients. PATIENTS AND METHODS: Two hundred and twenty seven consecutive dyspeptic Jordanian patients were studied with endoscopy, endoscopic biopsies, culture, and CLO urease testing for the detection of H. pylori. RESULTS: Helicobacter pylori positivity in both culture and CLO urease testing was 86%, being 78% in culture and 80% in CLO test separately. The majority of our patients were in the age range 21-60 years and H. pylori positivity was more than 90% in them. CONCLUSION: Helicobacter pylori is a common infection in dyspeptic Jordanian patients regardless of the underlying cause. Males were affected more than females.