Résumé
ABSTRACT Objective Intrathyroid injection of dexamethasone (IID) was used for decrease the relapse rate of hyperthyroidism in the treatment of Graves' disease (GD), but the mechanism is still unclear. We aimed to explore the effect of IID on T help (Th)1/Th2 cells and their chemokine in patients with GD. Subjects and methods A total of 42 patients with GD who were euthyroidism by methimazole were randomly divided into IID group (n = 20) and control group (n = 22). Thyroid function and associated antibody, Th1/Th2 cells proportion, serum CXCL10 and CCL2 levels, and CXCR3/CCR2 mRNA expression in peripheral blood mononuclear cells before and after 3-month IID treatment were tested by chemiluminescence assay, Flow cytometry, ELISA, and real-time PCR, respectively. Thyroid follicular cells were stimulated by IFN-γ and TNF-α and treated with dexamethasone in vitro. CXCL10 and CCL2 levels in supernatant were determined. Results After 3-month therapy, the proportion of Th2 cells and serum CCL2 levels, as well as TPOAb, TRAb levels and thyroid volume decreased in IID group (p < 0.05). However, the proportion of Th1 and CXCL10 levels had no change in IID group and control (p > 0.05). The CXCR3/CCR2 ratio had no change in both groups (p > 0.05). Conclusion IID therapy could inhibit peripheral Th2 cells via decreasing CCL2 level in peripheral blood, and this result partly explain the effects of IID therapy on prevention of relapse of GD. Arch Endocrinol Metab. 2020;64(3):243-50
Sujets)
Humains , Mâle , Femelle , Adulte , Dexaméthasone/analogues et dérivés , Maladie de Basedow/traitement médicamenteux , Lymphocytes auxiliaires Th2/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Anti-inflammatoires/administration et posologie , Récidive , Résultat thérapeutique , Prévention secondaire , Adulte d'âge moyenRésumé
The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4+ T cells and Th1 differentiation from CD4+ T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing Th1 differentiation. By up-regulating miR-142, artesunate suppressed Sirt1 level and promoted Th1 differentiation. Artesunate enhanced the pro-apoptotic effects of Th1 cells on ovarian cancer via the miR-142/Sirt1 pathway. Artesunate promoted Th1 differentiation from CD4+ T cells by down-regulating Sirt1 through miR-142, thereby enhancing cell apoptosis in ovarian cancer.
Sujets)
Animaux , Femelle , Lapins , Tumeurs de l'ovaire/traitement médicamenteux , Lymphocytes T CD4+/effets des médicaments et des substances chimiques , Apoptose , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , microARN/métabolisme , Artésunate/pharmacologie , Tumeurs de l'ovaire/immunologie , Lymphocytes T CD4+/cytologie , Régulation négative , Différenciation cellulaire , Lymphocytes auxiliaires Th1/cytologie , Cytométrie en flux , Artésunate/usage thérapeutique , Souris de lignée C57BL , Antinéoplasiques/usage thérapeutique , Antinéoplasiques/pharmacologieRésumé
BACKGROUND: CD4+ T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-γ (IFN-γ)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases. RESULTS: In the current study, we found that BJ-1108, a 6-aminopyridin-3-ol analogue, significantly inhibited Th1 and Th17 differentiation in vitro in a concentration-dependent manner, with no effect on proliferation or apoptosis of activated T cells. Moreover, BJ-1108 inhibited differentiation of Th1 and Th17 cells in ovalbumin (OVA)-specific OT II mice. A complete Freund's adjuvant (CFA)/OVA-induced inflammatory model revealed that BJ-1108 can reduce generation of proinflammatory Th1 and Th17 cells. Furthermore, in vivo studies showed that BJ-1108 delayed onset of disease and suppressed experimental autoimmune encephalomyelitis (EAE) disease progression by inhibiting differentiation of Th1 and Th17 cells. CONCLUSIONS: BJ-1108 treatment ameliorates inflammation and EAE by inhibiting Th1 and Th17 cells differentiation. Our findings suggest that BJ-1108 is a promising novel therapeutic agent for the treatment of inflammation and autoimmune disease.
Sujets)
Animaux , Femelle , Souris , Différenciation cellulaire/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Encéphalomyélite auto-immune expérimentale/traitement médicamenteux , Cellules Th17/effets des médicaments et des substances chimiques , Aminopyridines/pharmacologie , Dérivés de l'aniline/pharmacologie , Rate/immunologie , Lymphocytes T CD4+/effets des médicaments et des substances chimiques , Lymphocytes T CD4+/immunologie , Reproductibilité des résultats , Apoptose/effets des médicaments et des substances chimiques , Apoptose/immunologie , Lymphocytes auxiliaires Th1/immunologie , Prolifération cellulaire/effets des médicaments et des substances chimiques , Modèles animaux de maladie humaine , Encéphalomyélite auto-immune expérimentale/immunologie , Cellules Th17/immunologie , Cytométrie en flux , Aminopyridines/immunologie , Dérivés de l'aniline/immunologie , Noeuds lymphatiques/immunologie , Souris de lignée C57BLRésumé
Asthma is a chronic allergic disease characterized by airway inflammation, airway hyper-responsiveness (AHR), and mucus hypersecretion. T-lymphocytes are involved in the pathogenesis of asthma, mediating airway inflammatory reactions by secreting cytokines. The phosphoinositide 3-kinase (PI3K) and Notch signaling pathways are associated with T cell signaling, proliferation, and differentiation, and are important in the progression of asthma. Thus, compounds that can modulate T cell proliferation and function may be of clinical value. Here, we assessed the effects of tangeretin, a plant-derived flavonoid, in experimental asthma. BALB/c mice at postnatal day (P) 12 were challenged with ovalbumin (OVA). Separate groups of mice (n=18/group) were administered tangeretin at 25 or 50 mg/kg body weight by oral gavage. Dexamethasone was used as a positive control. Tangeretin treatment reduced inflammatory cell infiltration in bronchoalveolar lavage fluid (BALF) and also restored the normal histology of lung tissues. OVA-specific IgE levels in serum and BALF were reduced. AHR, as determined by airway resistance and lung compliance, was normalized. Flow cytometry analyses revealed a reduced Th17 cell population. Tangeretin reduced the levels of Th2 and Th17 cytokines and raised IFN-γ levels. PI3K signaling was inhibited. The expressions of the Notch 1 receptor and its ligands Jagged 1 and 2 were downregulated by tangeretin. Our findings support the possible use of tangeretin for treating allergic asthma.
Sujets)
Animaux , Souris , Asthme/traitement médicamenteux , Transduction du signal/effets des médicaments et des substances chimiques , Antiasthmatiques/usage thérapeutique , Flavones/usage thérapeutique , Asthme/immunologie , Cytokines/effets des médicaments et des substances chimiques , Cytokines/immunologie , Lymphocytes auxiliaires Th2/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th2/immunologie , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th1/immunologie , Modèles animaux de maladie humaine , Protéines proto-oncogènes c-akt/effets des médicaments et des substances chimiques , Protéines proto-oncogènes c-akt/immunologie , Cellules Th17/effets des médicaments et des substances chimiques , Cellules Th17/immunologie , Animaux nouveau-nés , Souris de lignée BALB CRésumé
Thymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro, and to evaluate its efficacy as an immunoregulatory factor in patients with gastric carcinoma. We compared the effect of Tα1 on the frequency of CD4+ and CD8+ T cells, especially the CD4+CD25+Foxp3+ Tregs in peripheral blood mononuclear cells (PBMCs) from gastric carcinoma patients (N = 35) and healthy donors (N = 22). We also analyzed the changes in the proliferation of PBMCs in response to treatment with Tα1, and examined the production of Th1, Th2, and Th17 cytokines by PBMCs and tumor-infiltrating lymphocytes. The treatment of PBMCs from gastric cancer patients, with Tα1 (50 µg/mL) alone increased the percentage of CD4+CD25+Foxp3+ (suppressive antitumor-specific Tregs) from 1.68 ± 0.697 to 2.19 ± 0.795 percent (P < 0.05). Our results indicate that Tα1 increases the percentage of Tregs and IL-1β, TNF-α, and IL-6 in vitro.
Sujets)
Adulte , Sujet âgé , Femelle , Humains , Adulte d'âge moyen , Jeune adulte , Antinéoplasiques/pharmacologie , Cytokines/effets des médicaments et des substances chimiques , Tumeurs de l'estomac/immunologie , Lymphocytes T auxiliaires/effets des médicaments et des substances chimiques , Lymphocytes T régulateurs/effets des médicaments et des substances chimiques , Thymosine/analogues et dérivés , Antinéoplasiques/immunologie , Antinéoplasiques/usage thérapeutique , Études cas-témoins , Prolifération cellulaire/effets des médicaments et des substances chimiques , Cytokines/immunologie , Cytométrie en flux , Lymphocytes TIL/effets des médicaments et des substances chimiques , Lymphocytes TIL/immunologie , Tumeurs de l'estomac/traitement médicamenteux , Lymphocytes T auxiliaires/immunologie , Lymphocytes T régulateurs/immunologie , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th1/immunologie , /effets des médicaments et des substances chimiques , /immunologie , /effets des médicaments et des substances chimiques , /immunologie , Thymosine/immunologie , Thymosine/pharmacologie , Thymosine/usage thérapeutiqueRésumé
Collagen-induced arthritis (CIA) is mediated by self-reactive CD4+ T cells that produce inflammatory cytokines. TGF-beta2-treated tolerogenic antigen-presenting cells (Tol-APCs) are known to induce tolerance in various autoimmune diseases. In this study, we investigated whether collagen-specific Tol-APCs could induce suppression of CIA. We observed that Tol-APCs could suppress the development and severity of CIA and delay the onset of CIA. Treatment of Tol-APCs reduced the number of IFN-gamma- and IL-17-producing CD4+ T cells and increased IL-4- and IL-5-producing CD4+ T cells upon collagen antigen stimulation in vitro. The suppression of CIA conferred by Tol-APCs correlated with their ability to selectively induce IL-10 production. We also observed that treatment of Tol-APCs inhibited not only cellular immune responses but also humoral immune responses in the process of CIA. Our results suggest that in vitro-generated Tol-APCs have potential therapeutic value for the treatment of rheumatoid arthritis as well as other autoimmune diseases.
Sujets)
Animaux , Souris , Cellules présentatrices d'antigène/effets des médicaments et des substances chimiques , Arthrite expérimentale/immunologie , Poulets , Collagène de type II/immunologie , Tolérance immunitaire/effets des médicaments et des substances chimiques , Souris de lignée BALB C , Ovalbumine/immunologie , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th2/effets des médicaments et des substances chimiques , Facteur de croissance transformant bêta-2/pharmacologieRésumé
Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-l©¬, IFN-¥ã, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-¥ã) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-¥ã/CCR5 and IL-1©¬/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1.
Sujets)
Femelle , Humains , Mâle , Cytokines/biosynthèse , Oligopeptides/pharmacologie , /effets des médicaments et des substances chimiques , /effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , /effets des médicaments et des substances chimiques , Cellules cultivées , Entamoeba histolytica/immunologie , Cytométrie en flux , Oligopeptides/biosynthèse , /immunologie , /immunologie , 12-Myristate-13-acétate de phorbol/pharmacologie , Lymphocytes auxiliaires Th1/immunologie , /immunologieRésumé
Pregnancy is not as successful as one might think; it can be compromised by several complications such as recurrent spontaneous miscarriage, pre-term delivery, pre-eclampsia etc. Much attention has been paid to the possibility of the maternal immune system mediating deleterious effects on pregnancy. Research conducted during the last two decades has shed much light on cell-mediated immunologic effectors that might underlie these pregnancy complications. Of particular interest are the effects that pro-inflammatory and anti-inflammatory cytokines have on the foetus and placenta, and thus on the success and failure of pregnancy. This review presents evidences that certain cytokine profiles are associated with recurrent miscarriage and pre-term delivery and discusses possible pathways of effector function of cytokines in pregnancy loss and the redirection of cytokine profiles from one that is antagonistic to pregnancy towards one that is conducive to the success of pregnancy. Among the promising agents for the modulation of the Th1/Th2 balance are progestogens like progesterone and dydrogesterone; this review also discusses recent evidence that progestogens are capable of modulating cytokine production patterns in pregnancy loss.
Sujets)
Avortement spontané/immunologie , Cytokines/biosynthèse , Dydrogestérone/pharmacologie , Femelle , Humains , Médiateurs de l'inflammation/pharmacologie , Grossesse , Complications de la grossesse/immunologie , Issue de la grossesse , Naissance prématurée/immunologie , Progestérone/pharmacologie , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th2/effets des médicaments et des substances chimiquesRésumé
The effect of soluble antigenic (bovine serum albumin, BSA) stimulation to induce steroidogenesis in murine lymphoid organs with concomitant changes in proinflammatory or inflammatory cytokine levels and its implication in the alteration of T-cell response was studied in the mice. Male Swiss albino mice (6-8 weeks old) with average body weight (20 +/- 4 g) were randomly assigned to 3 groups and injected with BSA in presence and absence of Freund's complete or incomplete adjuvant, whereas the control group received only saline. After 3 weeks, animals were sacrificed, and serums as well as lymphoid organs were collected. From the lymphoid tissue homogenate, the activities of steroidogenic enzymes and corticosterone and cytokine levels of the serum were estimated. Steroidogenic enzyme activities in murine lymphoid organs, as well as the pro-inflammatory and inflammatory cytokines levels in serum increased after Freund's complete adjuvant-emulsified BSA administration, as compared to control. The serum corticosterone and serum cytokine profile were also elevated. Results suggested that soluble protein antigen (BSA) administration stimulated steroidogenesis in murine lymphoid tissues and rise in the pro-inflammatory or inflammatory cytokine levels might indicate monocyte recruitment as well as TH1 activation.
Sujets)
17-Hydroxysteroid dehydrogenases/métabolisme , Adjuvants immunologiques , Animaux , Corticostérone/sang , Cytokines/sang , Adjuvant Freund/administration et posologie , Noeuds lymphatiques/enzymologie , Système lymphatique/effets des médicaments et des substances chimiques , Activation des lymphocytes/effets des médicaments et des substances chimiques , Mâle , Souris , Sérumalbumine bovine/administration et posologie , Rate/enzymologie , Stéroïdes/biosynthèse , Lymphocytes T/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Thymus (glande)/enzymologieRésumé
Colchicine has been shown to regulate the expression of inflammatory gene, but this compound possesses much weaker anti-inflammatory activity. In this study, we synthesized a new colchicine derivative CT20126 and examined its immunomodulatory property. CT20126 was found to have immunosuppressive effects by inhibiting lymphocyte proliferation without cytotoxicity and effectively inhibit the transcriptional expression of the inflammatory genes, iNOS, TNF-alpha, and IL-1beta, in macrophages stimulated by LPS. This effect was nearly comparable to that of cyclosporine A. This compound also significantly suppressed the production of nitric oxide and Th1-related pro-inflammatory cytokines, IL-1beta, TNF-alpha, and IL-2, with minimal suppression of Th2-related anti-inflammatory cytokines IL-4 and IL-10 in the sponge matrix allograft model. Moreover, administration of CT20126 prolonged the survival of allograft skins from BALB/c mice (H-2d) to the dorsum of C57BL/6 (H-2b) mice. The in vivo immune suppressive effects of CT20126 were similar to that of cyclosporine A. These results indicate that this compound may have potential therapeutic value for transplantation rejection and other inflammatory diseases.
Sujets)
Animaux , Femelle , Souris , Lignée cellulaire , Colchicine/analogues et dérivés , Cytokines/biosynthèse , Régulation de l'expression des gènes/effets des médicaments et des substances chimiques , Survie du greffon/effets des médicaments et des substances chimiques , Immunosuppression thérapeutique , Interleukine-1 bêta/génétique , Lipopolysaccharides/pharmacologie , Test de culture lymphocytaire mixte , Souris de lignée BALB C , Souris de lignée C57BL , Monoxyde d'azote/biosynthèse , Nitric oxide synthase type II/génétique , Transplantation de peau/immunologie , Lymphocytes auxiliaires Th1/effets des médicaments et des substances chimiques , Lymphocytes auxiliaires Th2/effets des médicaments et des substances chimiques , Transplantation homologue , Facteur de nécrose tumorale alpha/génétiqueRésumé
The cytokine pattern on viral antigen recognition is believed to exert a profound influence on the resolution of viral infections and viral clearance. This study was initiated to investigate whether a cytokine imbalance oriented toward Th2 type response plays a role in chronic hepatitis B. Cytokine profiles of peripheral blood mononuclear cells associated with chronic hepatitis B were analysed by RT-PCR. Upon HBsAg stimulation, expression of IFN-gamma, IL-2, IL-4, and IL-10 was detected in 41%, 8%, 41%, and 50% of the patients, respectively. Among these cytokines, the expression of IFN-gamma was associated with high levels of serum AST/ALT. However, we could not prove that Th2 type cytokines had a protective effect on hepatocytes. Upon HBxAg stimulation, there was no recognizable association of cytokine patterns with AST/ALT levels. In conclusion, production of a Th1 cytokine, IFN-gamma, by HBsAg-reactive cells was associated with hepatocyte damage in chronic hepatitis B, while no counteracting effect of Th2 cytokines produced by those cells was observed.