RÉSUMÉ
The bacterium, Inquilinus limosus, with its remarkable antimicrobial multiresistant profile, has increasingly been isolated in cystic fibrosis patients. We report draft genome sequence of a strain MP06, which is of considerable interest in elucidating the associated mechanisms of antibiotic resistance in this bacterium and for an insight about its persistence in airways of these patients.
Sujet(s)
Antibactériens/effets des médicaments et des substances chimiques , Antibactériens/génétique , Antibactériens/microbiologie , Antibactériens/pharmacologie , Séquence nucléotidique/effets des médicaments et des substances chimiques , Séquence nucléotidique/génétique , Séquence nucléotidique/microbiologie , Séquence nucléotidique/pharmacologie , Multirésistance bactérienne aux médicaments/effets des médicaments et des substances chimiques , Multirésistance bactérienne aux médicaments/génétique , Multirésistance bactérienne aux médicaments/microbiologie , Multirésistance bactérienne aux médicaments/pharmacologie , Génome bactérien/effets des médicaments et des substances chimiques , Génome bactérien/génétique , Génome bactérien/microbiologie , Génome bactérien/pharmacologie , Infections bactériennes à Gram négatif/effets des médicaments et des substances chimiques , Infections bactériennes à Gram négatif/génétique , Infections bactériennes à Gram négatif/microbiologie , Infections bactériennes à Gram négatif/pharmacologie , Humains/effets des médicaments et des substances chimiques , Humains/génétique , Humains/microbiologie , Humains/pharmacologie , Données de séquences moléculaires/effets des médicaments et des substances chimiques , Données de séquences moléculaires/génétique , Données de séquences moléculaires/microbiologie , Données de séquences moléculaires/pharmacologie , Rhodospirillaceae/effets des médicaments et des substances chimiques , Rhodospirillaceae/génétique , Rhodospirillaceae/microbiologie , Rhodospirillaceae/pharmacologieRÉSUMÉ
Abstract Acinetobacter baumannii is a frequently isolated etiologic agent of nosocomial infections, especially in intensive care units. With the increase in multi-drug resistance of A. baumannii isolates, finding appropriate treatment alternatives for infections caused by these bacteria has become more difficult, and available alternate treatments include the use of older antibiotics such as colistin or a combination of antibiotics. The current study aimed to evaluate the in vitro efficacy of various antibiotic combinations against multi-drug resistant A. baumannii strains. Thirty multi-drug and carbapenem resistant A. baumannii strains isolated at the Ankara Training and Research Hospital between June 2011 and June 2012 were used in the study. Antibiotic susceptibility tests and species-level identification were performed using conventional methods and the VITEK 2 system. The effects of meropenem, ciprofloxacin, amikacin, tigecycline, and colistin alone and in combination with sulbactam against the isolates were studied using Etest (bioMérieux) in Mueller-Hinton agar medium. Fractional inhibitory concentration index (FIC) was used to determine the efficacy of the various combinations. While all combinations showed a predominant indifferent effect, a synergistic effect was also observed in 4 of the 5 combinations. Synergy was demonstrated in 43% of the isolates with the meropenem-sulbactam combination, in 27% of the isolates with tigecycline-sulbactam, and in 17% of the isolates with colistin-sulbactam and amikacin-sulbactam. No synergy was detected with the sulbactam-ciprofloxacin combination and antagonism was detected only in the sulbactam-colistin combination (6.66% of the isolates). Antibiotic combinations can be used as an alternative treatment approach in multi-drug resistant A. baumannii infections.