Spectrophotometric determination of sertraline hydrochloride by ion pair complexation

Simple, sensitive and accurate Spectrophotometric method for the determination of Sertraline hydrochloride in pure samples and tablets is presented. An ion pair complexation with bromocresol purple acidic dye was carried out. Sertraline hydrochloride was treated with reagent bromocresol purple in the presence of Clark and Lubes buffer at pH 2.0. The ion pair complex was extracted with chloroform and the absorbance was measured at 412 nm. Molar absorptivity was about 2.50 x 10[4] L cm[-1] mol[-1]. The variables that affect development of color were investigated and the conditions were optimized. The proposed procedure was applicable to determine 2-14 microg / ml of Sertraline hydrochloride according to Beers law and the visual detection limit was 0.10 microg / ml. Correlation coefficient was 0.9993 which indicate good linearity. The stoichiometry of Sertraline hydrochloride - bromocresol purple complex was 1:1 as studied by Job's method of continuous variation. The mean percentage recoveries +/- SD were found to be 99.52 +/- 0.59 and 100.45 +/- 0.79 in pure form and tablets preparation respectively which indicate accurate and precise results. The validity of the method was checked by applying standard addition method; the mean percentage recovery was 99.76 +/- 0.60, this means there is no interferences from Excipients in tablets at 412 nm

Stability-indicating spectrophotometric method for determination of Moexipril-HCL in bulk and pharmaceutical formulation

Three simple, sensitive and accurate spectrophotometric procedures have been established for the assay of Moexipril-HCI in bulk form, in pharmaceutical formulations, and in the presence of its degradation products. The procedures are based on the reaction between the examined drug and bromocresol purple [BCP], bromophenol blue [BPB], and bromothymol blue [BTB] in aqueous acidic medium producing an ion-pair complexes extracted in chloroform and measured at the optimum wavelengths. Reaction conditions were studied and optimized to obtain the maximum color intensity. The reactions were extremely rapid at room temperature and the absorbance values remains unchanged for 48 h. Beer's law was obeyed in the concentration ranges 4-32, 4-24, and 4-40 microg ml[-1] with molar absorptivities of 1.7x10[4], 2.1x10[4], and 1.5x10[4] mol[-1] cm[-1] and detection limit of 0.064, 0.065, and 0.077 microg ml[-1] for BCP, BPB, and BTB methods, respectively. The proposed methods have been applied successfully for the analysis of the drug in pure form and in its dosage forms with percentage recoveries range from 99.29 - 100.11. Statistical comparison of the results with those obtained by second derivatives spectrophotometric method shows excellent agreement and indicates no significant difference in accuracy and precision

New Selective Medium for Rapid Identification of Vibrio vulnificus from Patients with V. vulnificus Sepsis / 대한피부과학회지

BACKGROUND: Vibrio(V.) vulnificus is a halophilic, gram-negative bacillus that causes a fatal sepsis in patients with underlying chronic disease such as liver cirrhosis and alcoholic abuse. Because V. vulnificus infection has a fulminant course and high mortality rate, early recognition and rapid diagnosis with prompt therapy are necessary to improve survival rate. OBJECTIVE: The purpose of this study was to develop a new selective medium for rapid identification of V. vulnificus through color change of medium according to pH from patients suspected of having V. vulnificus sepsis. METHODS: Rapid isolation and identification of V. vulnificus can be possible by modifying the component of PNC(5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) broth medium. From this PNC broth, a basal broth(5% peptone+1% NaCl+cellobiose) was prepared and used to evaluate additional medium supplements(cellobiose concentration [0.08, 0.2, 0.1%], pH [6.8, 7.5, 8.0] and pH indicator dye [bromthymol blue, thymol blue, phenol red, bromcresol purple, crystal violet, cresol red, and neutral red]). To examine the rapid identification and selectivity of this basal medium according to various conditions, V. vulnificus was tested by using saline and normal human blood containing these bacteria(1, 000 bacteria/ml), respectively at 37degrees C. A positive reaction(V. vulnificus growth) appeared as color change. The selectivity and identification capacity of this new broth was tested by using other 6 Vibrio species and 14 strains of other bacteria. RESULTS: Color change appeared only in the medium including bromthymol blue and thymol blue as a pH indicator dye. It was called the basal medium containing blue dyes as PNCB(peptone, NaCl, cellobiose and blue dye) medium. It took an average time of 4.8hr for becoming aware of yellow color change in PNCB broth after cultivating with saline mixed with V. vulnificus and 6hr in PNCB broth after cultivating with blood mixed with V. vulnificus. One Vibrio species and another 3 bacteria produced color change. So we confirmed that the final composition and pH of PNCB broth medium was 5% peptone, 1% NaCl, 0.2% cellobiose, 0.0004% bromthymol blue and 0.0004% thymol blue [pH 7.5] CONCLUSIONS: PNCB broth could be used as a selective and differential medium for rapid isolation and identification of V. vulnificus in patients with V. vulnificus sepsis.

Correlation of the suicide phenomenon in Aeromonas species with virulence and enteropathogenicity.

Certain strains of mesophilic Aeromonads like A. hydrophila, A. veronii biotype sobria and A. caviae when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. As these strains become nonviable after 24 h, this phenomenon is termed suicide. We investigated suicidal strains of Aeromonas species as means of understanding animal virulence and enteropathogenicity. Non suicidal strains of A. Hydrophila showed and overall 88.8% lethality rate and non suicidal strains of A. veronii biotype sobria showed 83.3% lethality rate and was nil for its suicidal part. Of the two suicidal A. caviae strains tested, none were lethal. The present data suggest that the suicide phenomenon may explain strain specific [A. veronii biotype sobria, A. hydrophila] and species specific [A. caviae] virulence and enteropathogenicity.

Determinaçäo de albumina humana em soros normais: comparaçäo de três métodos / Determinantion of human albumin in normal sera: comparison of 3 methods

A concentraçäo de albumina em soros de indivíduos normais foi determinada através dos métodos verde de bromocresol (VBC), púrpura de bromocresol (PBC) e imunoturbidimetria (I). A precisäo (Cv) destes métodos, investigada em "pool" de soros normais, foi de 3,6% para o imunoensaio e de 2,8% e 2,5% para o VBC e PBC, respectivamente. Estudos comparativos mostraram que os valores de albumina obtidos pelos métodos colorimétricos foram sistemática e significativamente mais elevados que os determinados por imunoturbidimetria. A PBC proporcionou valores de albumina mais próximos daqueles conferidos pela imunoturbidimetria, corroborando as evidências já apontadas na literatura a cerca da maior especificidade da reaçäo PBC-albumina