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1.
Clinics ; Clinics;67(10): 1171-1179, Oct. 2012. ilus, tab
Article de Anglais | LILACS | ID: lil-653481

RÉSUMÉ

OBJECTIVE: To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS: Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick test) with native and polymerized β-lactoglobulin in adult patients with an IgE-mediated allergy to plactoglobulin. In vitro humoral immunoreactivity was analyzed using immunoblotting. Cell-mediated immunoreactivity was analyzed using ex vivo challenges with native and polymerized β-lactoglobulin and monitored by leukocyte adherence inhibition tests. RESULTS: The skin tests demonstrated that there was a significant reduction in immediate cutaneous reactivity after polymerization. Polymerization did not decrease the immunoblotting detection of s-IgE specific to β-lactoglobulin. Cell-mediated immunoreactivity, as assessed by ex vivo challenges and leukocyte adherence inhibition tests, did not exhibit significant differences between leukocytes challenged with native versus polymerized β-lactoglobulin. CONCLUSIONS: The polymerization of β-lactoglobulin decreased in vivo allergenicity and did not decrease in vitro humoral or ex vivo cell-mediated immunoreactivity. Therefore, we conclude that inducing polymerization using transglutaminase represents a promising technique to produce suitable molecules for the purpose of designing oral/ sublingual tolerance induction protocols for the treatment of allergies.


Sujet(s)
Adolescent , Adulte , Sujet âgé , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Cystéine/immunologie , Tolérance immunitaire/immunologie , Lactoglobulines/immunologie , Hypersensibilité au lait/immunologie , Transglutaminases/immunologie , Allergènes/immunologie , Études cas-témoins , Cystéine/composition chimique , Chauffage , Immunotransfert , Immunoglobuline E/sang , Test d'inhibition de l'adhérence des leucocytes , Hypersensibilité au lait/prévention et contrôle , Polymérisation , Tests cutanés , Statistique non paramétrique , Transglutaminases/composition chimique
2.
Electron. j. biotechnol ; Electron. j. biotechnol;10(4): 618-626, oct. 2007. ilus, graf, tab
Article de Anglais | LILACS | ID: lil-504114

RÉSUMÉ

Finding a new microbial source of transglutaminase (MTGase) and the study of the medium composition for MTGase production were the goals of this work. A total of 200 actinomycete-like strains were isolated from Brazilian soil samples and two of them named T10b and P20 were selected based on their ability to produce 0.15 U.mL-1 and 0.25 U.mL-1 of MTGase, respectively. Strain P20 was chosen to continue the study and was identified as Streptomyces sp. In order to optimize the MTGase activity, modifications of the usual media composition described for enzyme production were tested. The strategy adopted was: (1) screening experiment for the best carbon and nitrogen sources; (2) fractional factorial design (FFD) to elucidate the key ingredients in the media (the results indicated that the soybean flour, peptone, KH2PO4 and MgSO4.7H2O had a significant effect on MTGase) production and (3) central composite design (CCD) to optimize the concentration of the key components. The experimental results were fitted to a second-order polynomial model at the 95 percent level of significance (P < 0.05). Under the proposed optimized conditions, the model predicted a MTGase activity of 1.37 U.mL-1, very closely matching the experimental activity of 1.4 U.mL-1.


Sujet(s)
Streptomyces/enzymologie , Transglutaminases/composition chimique , Analyse de variance , Actinobacteria/isolement et purification , Fractionnement chimique , Milieux de culture , Fermentation , Modèles biologiques , Viscosité
3.
Exp. mol. med ; Exp. mol. med;: 177-185, 1998.
Article de Anglais | WPRIM | ID: wpr-159772

RÉSUMÉ

The relevance of transglutaminases with neural function and several disorders has been emphasized recently. Especially, many polypeptides associated with neurodegenerative diseases are suggested to be putative transglutaminase substrates such as beta amyloid protein of Alzheimer's disease, microtubule-associated proteins and neurofilaments, etc. In addition, the CAG repeated gene products with probable polyglutamine tract, putative transglutaminase substrates, were identified in several neurodegenerative disorders. However, the identity of the brain transglutaminase has not been confirmed, because of enzymic stability and low activity. In the present experiment, we have isolated brain-specific transglutaminases, designated as TGase NI and TGase NII, which are different from other types of transglutaminases in respects of molecular weights (mw. 45 kDa, 29 kDa respectively), substrate affinity, elution profile on ion-exchange chromatography, sensitivity to proteases and ethanol, and immunological properties. The enzymes were localized specifically in the brain tissues but not in the liver tissue. And neural cells such as pheochromocytoma cell, glioma cell, primary neuronal and glial cells were shown to be enriched with TGase NI and TGase NII. The possible biological roles of the enzymes were discussed not only on the aspect of crosslinking activity but also of signal transducing capacity of the enzyme in the brain.


Sujet(s)
Mâle , Rats , Animaux , Astrocytes/enzymologie , Technique de Western , Encéphale/enzymologie , Calcium/métabolisme , Chromatographie d'échange d'ions , Endopeptidases/pharmacologie , Stabilité enzymatique , Éthanol/pharmacologie , Gliome , Immunotransfert , Immunohistochimie , Masse moléculaire , Neurones/enzymologie , Cellules PC12 , Transglutaminases/isolement et purification , Transglutaminases/immunologie , Transglutaminases/composition chimique , Rat Sprague-Dawley , Trypsine/pharmacologie , Cellules cancéreuses en culture
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