RESUMO
Protinases contained in the mid-gut of the early third instar of Gasterophilus intestinals have been tentatively identified by midgut hydrolysis of synthetic substrates. Trypsin was identified by maximal hydrolysis of benzoyl-DL-arginine-p-nitroanilide [BApNA] at pH 8 and chymotrypsin by maximal hydrolysis of benzoyl-L-tyrosine ethyl ester [BTEE] at pH 9. Carboxypeptidase A and B were identified by their maximal hydrolysis of hippuryl-DL-phenyllactic acid and hippuryl-L-arginine at pH 9 and 8 respectively. Aminopeptidase was identified by maximal hydrolysis of leucine-p-nitroanilide at pH 9. The mid-gut also showed activity of aspartic proteinase and identified it as cathepsin D.A drug [Banmith 12.5% pyrantel tartarate] used for the routine control of helminthes parasites of horses and donkies in Egypt was used in vitro to investigate its effect on the optimal activity of studied enzymes. It was found that the drug has no effect on trypsin and carboxypeptidase A while it decreases the activity of chymotrypsin, aminopeptidase and acidic proteinase and was also found to increase the activity of carboxypeptidase B greatly
Assuntos
Larva/enzimologia , Endopeptidases , Trato Gastrointestinal , Cavalos , Equidae , Quimotripsina , Tripsina , Aminopeptidases , CarboxipeptidasesRESUMO
Mid-gut homogenates from the early third larval instars of Gasterophilus intestinalis contain at least four glycosidases. Enzyme activity was found alpha-galactosidase and beta-galactosidase. Also-alpha-amylase showed activity in midcythomogenates. All enzymes had acidic pH optima of 3.6-6.A drug [Banminth 12.5% pyrantel tartarate] used for the routine control of helminthes parasites of horses and donkies in Egypt was used in vitro to investigate its effect on the optimal activity of studied enzymes
Assuntos
Larva/enzimologia , Glicosídeo Hidrolases , Trato Gastrointestinal , Cavalos , Equidae , Carboidratos , Concentração de Íons de HidrogênioAssuntos
Insetos , Pupa , Larva , Dípteros , Proteínas Alimentares , Necessidades Nutricionais , Ferro da Dieta , Sódio na Dieta , Potássio na DietaRESUMO
The effect of steady magnetic fields of different strength and orientation, on the ionic contents of Mosquito Larvae [Culex Pipiens] was studied. The data indicate that the Na, K, Ca, Mg, Fe and Cl content of the whole insects are under the controlled magnetic field, is highly affected by both the distribution of magnetic lines of force and the strength of the field