RESUMO
We report an outbreak of abortion due to equine herpesvirus (EHV) in 5 mares between 9 and 11 months of gestation, from a herd of 22 Thoroughbred mares. Equine herpesvirus was isolated from extracts of the liver, spleen and thymus but not from the lungs of a 9-month fetus grown in Rabbit Kidney (RK13) cells. The virus was identified by electron microscopy, where virus particles could be seen in the nucleus of infected cells, and by the fluorescent antibody technique with polyclonal antibodies against the whole virus. Anamnesis, necropsy, histopathology, bacteriology, and virology data suggest that the abortions reported in this paper were due to equine herpesvirus.
Assuntos
Animais , Feminino , Gravidez , Aborto Animal , Doenças dos Cavalos/virologia , Herpesvirus Equídeo 1 , Infecções por Herpesviridae/veterinária , Aborto Animal , Anticorpos Antivirais , Baço/patologia , Baço/virologia , Brasil , Doenças dos Cavalos/epidemiologia , Feto , Fígado/patologia , Fígado/virologia , Herpesvirus Equídeo 1 , Cavalos , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/prevenção & controle , Infecções por Herpesviridae/virologia , Microscopia Eletrônica , TimoRESUMO
We describe an acute outbreak of balanoposthitis in bulls at an artificial insemination station in South Brazil. Bovine herpesvirus was isolated from preputial swabs in the Crandell feline kidney cell line and secondary fetal bovine lung cells and identified using the fluorescent antibody technique and electron microscopy. Polyclonal antibodies against the whole virus were used for identification of the bovine herpesvirus with the fluorescent antibody technique. Herpesvirus particles could be seen in the infected cells by electron microscopy. Eleven bulls had clinical signs resembling balanoposthitis and nine yelded virus