rapid high performance liquid chromatographic determination of lansoprazole in human serum

A simple and rapid reverse phase high-performance liquid chromatographic [RP-HPLC] method with UV detection has been described for the determination of lansoprazole in human serum. Carbamazepine was used as internal standard. The drug and the internal standard in serum were extracted twice with diethyl ether, followed by evaporation, reconstitution in the mobile phase and injection into the chromatographic system. The method utilized a Nova-Pak CI8 4-micro m column [150x3.9 mm i.d.] together with an isocratic mobile phase consisted of 0.02M sodium dihydrogenphosphate- acetonitrile- methanol [58: 23: 19%, v/v/v]. The mobile phase was adjusted to pH 7.3 with 5M NaOH and pumped at a flow rate of 1.8 mI/min. The UV detector was set at 285 Rm. Running time per single analysis was less than four minutes. The response of the assay was linear with a correlation coefficient, r = 0.9993. The within and between-day coefficients of variation for three different concentrations [50-1500 ng/ml] ranged from 1.14 to 8.26% and from 1.66 to 8.02%, respectively. The average recovery of the concentration range stated was better than 96.5%. Stability testing revealed that lansoprazole was stable in serum at -20°C for two weeks. The method was successfully applied in a bioassay study of two products each in the form of enteric-coated granules in capsules containing 30 mg lansoprazole, administered orally to eighteen healthy male volunteers

Occurrence and some properties of a virus inhibitors extracted from legume seeds

Seed extracts from 18 species of the family Leguminosae were tested for inhibitory activity against tobacco necrosis virus [TNV] onto Phaseolus vulgaris L. Aqueous crude seed extracts of all the species were inhibitory to virus infection but to varying degrees. Twelve out of 18 examined species showed potent inhibition [80 - 98%]. The inhibitory activity of the majority of species was decreased on dilution [1: 1000] suggesting that they act as virus inhibitors and not inactivators. Further dilution of the extracts of a few species revealed the presence of an inhibitor which decreased and an augmenter which increased the number of lesions. The heated seed extracts fall into four categories: Those which are thermolabile inhibitors, those which are thermostable inhibitors, those which are thermostable augementers and those which inhibitory activity increased by heating. Ethanol treatment indicated that the inhibitors of all tested extracts were composed, at least in part, of proteins or glycoproteins

Mode of action of tobacco necrosis virus inhibitors extracted from seeds of enterolobium cyclocarpum and phaseolus vulgaris

Aqueous seed extracts prepared from two species belonging to the family Leguminosae [Enterolobium cyclocarpum Griseb and Phaseolus vulgaris L] were inhibited local lesion production by tobacco necrosis virus [TNV]. Dilution of both extracts confirmed the presence of plant virus inhibitors and not inactivators. Inhibition was slightly decreased by heating E. Cyclocarpum seed extracts, while the inhibitory activity of P. Vulgaris seed extracts was increased by heating. The virus inhibitors from both extracts had no direct effect on the virus. The virus inhibitors from both extracts had no direct effect on the virus. They seem either to affect the attachment of the virus to the infective sites or perhaps allow attachment but prevent entry of virus into the cell

Posterior approach for posterolateral cervical disc prolapse

This study was carried out on 12 cases of posterolateral cervical disc prolapse as proved by CT myelography or MRI of the cervical spine. All had presented with unilateral single level radiculopathy [commonly pain and uncommonly weakness]. All had been operated upon posteriorly via a limited fenestrum and discectomy. Patients had improved and pain [or weakness] resolved completely

Biochemcal studies of hepatocarcinogenesis in rats treated with chemical carcinogens

The present study was conducted on rats receiving nitrosamines precursors in their drinking water, namely 2000 ppm dibutylamine and 4000 ppm nitrite, to follow up biochemical changes that may occur in the growth of liver tumours. Impairment of liver function tests; transaminases and protein pattern occurred 2 months following carcinogenesis.Also, highly significant increase in the activity of several enzyme e.g. glucose -6-phosphate, Y- glutamyltransferase, 5- nucleotidase, acid and alkaline phosphatase were observed in both serum and liver tissue of these treated rats and were shown to parallel with continued carcinogesis moreover, high elevation in DNA content was also observed in liver tissue of rats with hepatocarcinoma and reached 1.6, 1.7 and 1.8 times that of control rats after 2, 4 and 7 months respectively. In contrast, highly significant decrease were observed in hepatic glycogen content and serum glucose level. These observations may be of value for confirming the detection and diagnosis of liver metastasis