effects of dietary Egyptian propolis and bee pollen supplementation against toxicity of sodium fluoride in rats

Propolis and bee pollen are substances produced by honey bees. Its components are strong antioxidants and free radical scavengers. The present study aimed to study the protective effects of propolis and bee pollen supplementation against toxicity of sodium fluoride [F] in rats. Rats were divided into six groups each 10 rats and treated for 6 weeks: group 1 as control group; group 2 fed on standard diet with F; groups [3-6] fed on standard diet with F and supplemented with different concentration of propolis or bee pollen at 0.1%, 0.2% and 1%, 2% respectively. After the end of experimental period, the rats were sacrified and biochemical analyses were carried out. The results showed that the administration of fluoride alone caused a significant increase of malondialdehyde [MDA] level and a significant decrease of antioxidant system as measured by erythrocyte superoxide dismutase [SOD] activity and reduced glutathione [GSH] levels in blood and brain. Also F caused a significant increase in alkaline phosphatase [ALP] activity, urea, creatinine, sodium and potassium levels.Moreover, a significant decrease in total protein, calcium, magnesium and phosphorus levels as compared to control group [P < 0.05] was recorded. The administration of propolis or bee pollen with F led to a significant decrease in MDA level and a significant increase in SOD activity, GSH levels in blood and brain.As well as significant decrease in ALP activity, urea, creatinine, sodium and potassium levels in serum was also observed. The propolis or bee pollen supplementation enhanced total protein, calcium, magnesium and phosphorus levels in serum as compared to F group alone. supplementation of natural antioxidants [propolis or beepollen] during Fluoride administration, facilitates reduction of the toxic effects and enhanced both the antioxidant system, as well as the levels of minerals in the serum

Ameliorative effect of wheat germ and/or grape seed oils on hematological, kidney functions and lipid profiles in rats co-administreted chlorpyrifos

Organophosphorous pesticides such as chlorpyrifos [CPF] are substances used worldwide for agricultural purposes. CPF induce oxidative stress leading to the generation of free radicals. The purpose of this study was to assess the biochemical role of wheat germ and grape seed oils on the kidney function tests and the oxidative stress alteration induced by chlorpyrifos in rats, moreover the heamatological, lipid profile. Throughout this study a total number of 70 rats were subjected to experimentation for 4 weeks and divided into 7 groups. The results demonstrated that there were significant decrease in the total counts of RBC's, WBC's, erythrocyte indices, hemoglobin concentration and hematocrit level in experimental rats fed diets containing low and high levels of CPF. CPF caused a significant increase in serum creatinine and urea the increase reached 211.39% and 104.29% respectively for high dose of CPF received rats as compared to control rats. The treatments with, wheat germ oil, grape seed oil at the tested doses significantly reduced serum creatinine by 27.84% and 15.19% for G6 and G4 respectively. Concerning the values of lipid profile measurement, it was clear that low and high dose CPF received rats induced a significant increase in the level of serum total lipid, total cholesterol and triacylglycerols, also this reflects an elevation in the level LDL-C, VLDL-C and LDL/HDL ratio. Meanwhile, wheat germ oil supplementation reduced the serum LDL-C, VLDL-C and LDL/HDL ratio and increased serum HDL-C. It can be concluded that wheat germ oil and grape seed oils, used are valuable natural antioxidants for protecting against oxidative toxic effects of CPF and cell damage caused by toxic chemicals

Impact of administration mulberry juice on blood glucose, lipid profile and oxidative stress in normal and diabetic rats

The aim of the present study was designed to study the effects of black and white mulberry juice on blood glucose, lipid profile and oxidative stress in normal and diabetic rats. Sprague Dawley male rats weighing [125 +/- 12.2 g] were divided into eight groups [each of 12 rats]. Four healthy control group include normal control, normal black mulberry, normal white mulberry and normal mixture of black and white mulberry, while the remaining four groups were induced diabetic rats by injected intraperitonelly with 100 mg/kg body weight of alloxan [diabetic control, diabetic received black mulberry, diabetic received white mulberry and diabetic received mixture of black and white mulberry]. Results show significant elevation in serum glucose [280%] in diabetic control group compared to normal control group. while administration of mulberry juice decreased serum glucose concentration by 45%, 20% and 37% in diabetic black mulberry, diabetic white mulberry and diabetic mixture mulberry, respectively as compared to diabetic control group compared to diabetic control group. However, the mulberry juice significantly increase the concentration of reduced glutathione [GSH] and erythrocyte SOD activity. Also mulberry juice significantly decrease oxidative stress which manifested by decrease the level of MDA and NO. The alloxan produced significant increase in ALT and AST, urea and creatinine in diabetic control group as compared to normal control group. While, the administration of mulberry juice decreased the levels of ALT, AST, urea and creatinine in diabetic group. However mulberry juice is the highly effective on lipid profile and atherogenic index which were manifested by decrease [TAG], TC, LDL, and atherogenic index and increase [HDL-C] as compared to diabetic control group [P<0.05]. It was concluded that the mulberry juice has considerable hypoglycemic and hypocholestronic effect and strengthening antioxidant, it able to reduce diabetic complications especially black mulberry, to rich in anthocyanin

Influence of dietary mustard and fennel seeds on hypercholesterolemic, hyperlipidemic rats

This work has been planned to study the effect of dietary mustard [Brassica campestris] and Fennel [Foenicum vulgare Mill] seeds on hypercholesterolemic rats. The male Albino rats with average weight [113 +/- 19.1 g] divided into six groups, group [1] as control [healthy] group; group [2] as high fat cholesterol; groups [3, 4] which rats fed on high fat cholesterol plus 5 or 10% mustard seeds; groups [5. 6] which rats fed on high fat cholesterol plus 5 or 10% fennel seeds respectively. Results obtained revealed that there were highly significant decrease in the mean values of total lipids, total cholesterol, triacylglycerols, VLDL-C, HDL-C, LDL-C and risk ratio. There was highly significant increase in concentration of glutathione [GSH] and superoxide dismutase [SOD] activity while highly significant decrease in malondialdehyde [MDA] as lipid peroxidation. Also results showed highly significant decrease in activities of ALT, AST and ALP and concentration of albumin and creatinine in serum as compared with group [2] fed on high fat cholesterol diet alone [P < 0.01]. It could be concluded that the administration of mustard or fennel seeds at levels of 5 and 10% in high fat cholesterol diet can be able to reduce the lipid profile and lipid peroxidation and maintain kidney and liver in rats

potential effect of lactobacillus plantarum on health

The potential effect of Lactobacillus plantarum as a probiotic bacteria in controlling hypercholesterolaemia was studied. Lactobacillus plantarum was supplied in a single form or in mixture with Lactobacillus paracasei or other strains of bacteria. Rats consumed these microorganisms daily for 42 days. Eighty eight rats were divided into eleven homogeneous groups, each group consists of eight male albino rats, Sprague Dawley strain, weight ranged from [105-117.5 g]. All groups were fed on basal diet supplemented with high fat, high cholesterol [15% saturated fat + 1% cholesterol + 0.2% bile salt] except group [1] which fed on high fat only [15% saturated fat]. Results obtained revealed that the mixed groups had a significant decreasing effect on gain in body weight and feed intake/day higher than single groups. While they had a higher significant decreasing effect on total lipid, total cholesterol, triacylglycerol of serum and liver, also had a higher significant increasing effect on serum HDL-C than the single groups. These results indicate that Lactobacillus plantarum had a high significant decreasing effect and when mixed with the other strains to produce mixed groups [G8-G11]; increased their hypocholesterolaemia effect more than these strains in a single form [G4-G7]. It could be concluded that the supplementation with different probiotic bacteria as Lactobacillus plantarum, Lactobacillus paracasei and other organisms to control hypercholesterolaemia, consequently, to avoid the risk of coronary heart disease

Effect of some dietary nutritive sweeteners on healthy and diabetic rats

The present work was performed to study the effect of feeding three different sweeteners on healthy and alloxan-diabetic rats. Thirty-six female albino rats [mean weight was 147.9 +/- 16.83 g] were divided into six homogenous groups, of which three control groups and three alloxan diabetic groups were included. All groups were fed on basal diet in combinations with 10% of either sucrose, fructose or sucrol. Food and tap water were provided ad libitum for 28 days. It was concluded that sucrose, a high glycemic index, should be provided in most of the non-insulin-dependent diabetic patients. The availability of sucrol may enable the patient to substationally liberalize the diabetic diet