RESUMO
Objective:To develop and evaluate a rapid and sensitive point-of-care chemiluminescent assay(POC-CLIA)for β-human chorionic gonadotropin(β-HCG).Methods:POC-CLIA was constructed based on alkaline phosphatase(Alp)-AMPPD lumi-nescence system and magnetic particles(Mps)carrier.Performance of POC-CLIA,including sensitivity,precision,accuracy,linear dilution,specificity,stability,hook effect and clinical application were evaluated.Results:Detection limit of β-HCG was 0.71 mU/ml,linear detection range was 0.710～1.092×104 mU/ml,and was no hook effect up to 1.7×105 mU/ml.Intra and inter batch coefficients of variation were less than 10％,and could be stored stably at 37℃ for 10 days.Accuracy deviation was within±10％,so results were reliable.There was no cross-reactivity between interfering substances and anti-β-HCG antibdies.For detecting β-HCG in 100 clinical serum samples,results were highly correlated with those that were tested by clinical standard methods(R2=0.997 0).Turnaround time for single sample was less than 15 min and throughput could reach 200 T/h.Conclusion:This method is adequate that can be widely used in grassroots communities to help large-scale screening of pregnancy and related diseases.
RESUMO
Prosthesis revision and service life after artificial joint replacement is highly correlated with the biocompatible responses among prosthesis per se, host, and manipulating technique. Artificial prosthesis consists of two parts: metal part and rigid plastic components. Many metals such as stainless steel, cobalt-chrome-molybdenum alloy and titanium alloy can be used as prosthesis materials. The plastic materials mainly include wear-resisting polythene, plastic bone cement that is used to embed the prosthesis into osseous substance. Host defines as the patients with various joint injury, mainly the elderly. Anesthesia is a key step in replacement. Artificial joint replacement is performed in patients under anesthesia. Different anesthesia methods bring variable influences on biocompatibility after surgery. In addition, complications of anesthesia may aggravate tissue lesions around operation sites in the early stage after replacement, leading to joint loosening or inadequate joint range of motion. Appropriate anesthesia, modified artificial joint design and joint replacement technique can prolong the serve life of artificial joint.