Exploring the teaching mode about clinical laboratory diagnostic pathway for chronic hepatitis B / 中华预防医学杂志

Exploring a new teaching mode of CHB laboratory diagnostics to improve the teaching quality through establishment a teaching model covered the whole process of CHB disease diagnosis and differential diagnosis, treatment, drug selection, the toxicity and side effects prediction, effect monitoring, and prognosis evaluation. According to the CHB clinical diagnosis and treatment guidelines, formulated the laboratory examination and detection strategies related to different stages of CHB, and established CHB clinical laboratory diagnostic pathway. Compared the classroom teaching effect by the questionnaire between the 2016 and 2017 eight-year undergraduates from the First Clinical College of Wuhan University. In this study,the CHB clinical laboratory diagnostic pathway was established and approved by clinicians, which covered the whole process of CHB disease diagnosis and differential diagnosis, treatment, drug selection, the toxicity and side effects prediction, effect monitoring, and prognosis evaluation. The teaching quality evaluation indicators and the scores on the class test had been greatly improved with the clinical diagnostic pathway teaching mode in the classroom teaching of 2017 clinical medicine undergraduates compared with the traditional teaching mode in the 2016 clinical medicine undergraduates. In summary, the medical students not only could realize the organic integration of laboratory diagnostics and clinical medicine, but also improves overall understanding of various laboratory tests in CHB diagnosis and treatment from the teaching model of laboratory diagnostics based on the CHB clinical laboratory diagnostic pathway,and the quality of teaching for CHB has been significantly improved.

Identification and antibacterial activity of three mangrove-derived Streptomyces / 中国药理学通报

Aim To study and identify the antibacterial activity of three strains of Streptomyces (K57M, K51M, K131M) collected from the roots of mangrove rhizosphere in Maowei Sea, Guangxi. Methods Acti-nobacteria were isolated on four media and purified by ISP2 medium. The 16S rRNA gene sequences of three actinobacteria strains were obtained by PCR amplification and sequencing. Sequences of 16S rRNA gene obtained by PCR amplification were submitted to gene database for BLAST search. Phylogenetic tree was constructed by adjacency method, and homology analysis was carried out to study the diversity and novelty of actinobacteria. Three actinobacteria strains underwent primary screening using Oxford cup method, and the antimicrobial activity of the sample of El-3, AI-3 and Ml-3 received secondary screening using disk diffusion method. Results Sequence alignment analysis of 16S rRNA gene revealed that strain K57M might be a potential new actinobacteria. The results of screening for antimicrobial activity showed that three strains of Streptomyces, K57M, K51M and K131M, had good antimicrobial activities against different drug-sensitive strains. Conclusions Streptomyces from rhizosphere in the roots of mangrove forests in Guangxi can produce strong antibacterial activities, which provides great possibilities to explore natural active antibiotics.

Genetic analysis of the PKHD1 gene with long-rang PCR sequencing / 华中科技大学学报（医学）（英德文版）

PKHD1 gene mutations are found responsible for autosomal recessive polycystic kidney disease (ARPKD). However, it is inconvenient to detect the mutations by common polymerase chain reaction (PCR) because the open reading frame of PKHD1 is very long. Recently, long-range (LR) PCR is demonstrated to be a more sensitive mutation screening method for PKHD1 by directly sequencing. In this study, the entire PKHD1 coding region was amplified by 29 reactions to avoid the specific PCR amplification of individual exons, which generated the size of 1 to 7 kb products by LR PCR. This method was compared to the screening method with standard direct sequencing of each individual exon of the gene by a reference laboratory in 15 patients with ARPKD. The results showed that a total of 37 genetic changes were detected with LR PCR sequencing, which included 33 variations identified by the reference laboratory with standard direct sequencing. LR PCR sequencing had 100% sensitivity, 96% specificity, and 97.0% accuracy, which were higher than those with standard direct sequencing method. In conclusion, LR PCR sequencing is a reliable method with high sensitivity, specificity and accuracy for detecting genetic variations. It also has more intronic coverage and lower cost, and is an applicable clinical method for complex genetic analyses.