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BACKGROUND:Abnormal Notch1 signaling pathway is mostly found in the brain of Alzheimer's disease patients,but the role of these signaling pathways in the pathogenesis of Alzheimer's disease has not been fully clarified.Long-term aerobic exercise can alter the expression of Notch1 by affecting the methylation rate of factors related to the Notch1 signaling pathway.However,it is not clear whether aerobic exercise affects hippocampal nerve cell proliferation and histopathological features of Alzheimer's disease mice through the Notch1 signaling pathway. OBJECTIVE:To observe the effects of aerobic exercise on the proliferation and histopathological features of hippocampal nerve cells in Alzheimer's disease mice after DAPT inhibited the Notch1 signaling pathway. METHODS:APP/PS1 double transgenic Alzheimer's disease mice aged 3 months were randomly divided into four groups:control group,exercise control group,inhibitor group,and exercise inhibitor group,with 20 mice in each group.The control group was fed naturally,and the exercise group received aerobic exercise intervention.Both natural feeding and exercise intervention lasted for 20 weeks.The mice were injected with solvent or Notch1 inhibitor at week 18.After 20 weeks,the brain tissue was collected,and Aβ1-42,Tau,Ki67,and Notch1 expression levels were detected by real-time PCR,immunofluorescence,and western blot assay. RESULTS AND CONCLUSION:Compared with the control group,the expressions of Ki67 and Notch1 in the dentate gyrus region of the hippocampus were significantly decreased in the inhibitor group(P<0.05),but there were no significant differences in Aβ1-42 and Tau.The expression of Ki67 in the dentate gyrus region of the hippocampus in the exercise control group was significantly higher than that in the control group,while the expressions of Aβ1-42,Tau,and Notch1 were significantly lower than those in the control group(P<0.05).The expressions of Aβ1-42,Tau,Ki67,and Notch1 in the dentate gyrus region of the hippocampus of the exercise inhibitor group were not significantly different from those of the inhibitor group.In conclusion,the Notch1 signaling pathway may mediate exercise to improve the proliferation and histopathological features of hippocampal nerve cells in Alzheimer's disease mice.
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Objective:To investigate the effect of applying narrative therapy theory into game on improving the quality of bowel preparation in children, and to provide a basis for selecting nursing intervention methods before bowel preparation.Methods:This study was a quasi experimental study. Totally 62 children who took polyethylene glycol electrolyte dispersion for bowel preparation in Gansu Maternal and Child Health Hospital from January to December 2021 were included in this retrospective study. They were divided into control group with 28 cases and experimental group with 34 cases according to random number table method. The control group was given conventional bowel preparation and medication guidance, and the experimental group was given the intervention during bowel preparation by applying narrative therapy theory implanted games. The bowel preparation adequacy rate, complete medication taking rate and parental satisfaction of the two groups were observed and compared.Results:The bowel preparation adequacy rate and complete medication taking rate in the experimental group were 94.12% (32/34) and 52.94% (18/34), respectively, which were higher than 46.43% (13/28) and 10.71% (3/28) in the control group, and the differences were statistically significant ( χ2 = 15.23, 10.41, both P<0.01). According to the average BBPS score, the experimental group had better intestinal cleanliness compared to the control group (7.65 ± 1.07 vs 6.07 ± 1.41, t = -4.87, P<0.01), the difference was statistically significant. 97.06% (33/34) of the parents in the experimental group expressed satisfaction, which was higher than 64.29% (18/28) in the control group, and the difference was statistically significant ( χ2 = 12.74, P<0.05). Conclusions:By applying the narrative therapy theory implanted games, the complete taking of high-dose bowel cleasing agent can be promoted and the quality of bowel preparation can be improved, which is worth suggesting as a nursing intervention method for bowel preparation.
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Objective:To investigate the relationship between the expression level of thymocyte selection-associated high mobility group box protein ( TOX) gene and the radiosensitivity of lower-grade glioma (LGG) patients. Methods:Using bioinformatics research methods, 474 LGG patients from The Cancer Genome Atlas (TCGA) database were selected as the test set (TCGA-474 set), and two different genetic data sets ( n=412 and n=171) from the Chinese Glioma Genome Atlas (CGGA) database were selected as the validation set (CGGA-412 set and CGGA-171 set). Patients were stratified based on whether received radiotherapy, and divided into the high and low TOX expression group according to the expression level of TOX gene in LGG. Survival curves of all patients were plotted. The overall survival (OS) and progression-free survival (PFS) of patients in the high and low TOX expression groups were compared and analyzed using log-rank test. Results:Multivariate analysis of OS in the TCGA-474 set showed that high expression of TOX was a protective factor for OS ( HR=0.061, 95% CI: 0.005-0.791, P=0.044). After stratification analysis based on radiotherapy and adjustment for confounding factors, the HR (95% CI) of patients with high TOX expression in the TCGA-474, CGGA-412, and CGGA-171 sets were 0.405 (0.261-0.629), 0.581 (0.418-0.806), and 0.464 (0.269-0.800), respectively, with P values of <0.001, 0.001, and 0.008, respectively. Among patients receiving radiotherapy in the TCGA-474 set, the OS and PFS of patients with high TOX expression were significantly longer than those in the low TOX expression group, and the differences were statistically significant (both P<0.001). The OS benefit of patients with high expression of TOX was significantly prolonged in both the CGGA-412 and CGGA-171 sets compared to those with low TOX expression, and the differences were statistically significant (both P<0.001). Conclusion:The high expression of TOX may be related to the radiosensitivity of LGG, which may be a gene marker of the radiosensitivity of LGG.
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Poria is an important medicine for inducing diuresis to drain dampness from the middle energizer. However, the specific effective components and the potential mechanism of Poria remain largely unknown. To identify the effective components and the mechanism of Poria water extract (PWE) to treat dampness stagnancy due to spleen deficiency syndrome (DSSD), a rat model of DSSD was established through weight-loaded forced swimming, intragastric ice-water stimulation, humid living environment, and alternate-day fasting for 21 days. After 14 days of treatment with PWE, the results indicated that PWE increased fecal moisture percentage, urine output, D-xylose level and weight; amylase, albumin, and total protein levels; and the swimming time of rats with DSSD to different extents. Eleven highly related components were screened out using the spectrum-effect relationship and LC-MS. Mechanistic studies revealed that PWE significantly increased the expression of serum motilin (MTL), gastrin (GAS), ADCY5/6, p-PKAα/β/γ cat, and phosphorylated cAMP-response element binding protein in the stomach, and AQP3 expression in the colon. Moreover, it decreased the levels of serum ADH, the expression of AQP3 and AQP4 in the stomach, AQP1 and AQP3 in the duodenum, and AQP4 in the colon. PWE induced diuresis to drain dampness in rats with DSSD. Eleven main effective components were identified in PWE. They exerted therapeutic effect by regulating the AC-cAMP-AQP signaling pathway in the stomach, MTL and GAS levels in the serum, AQP1 and AQP3 expression in the duodenum, and AQP3 and AQP4 expression in the colon.
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Animais , Ratos , Poria , Baço , Albuminas , Cromatografia Líquida , Proteína de Ligação ao Elemento de Resposta ao AMP CíclicoRESUMO
Objective:To investigate the efficacy and safety of SIMPLE regimen in the treatment of extranodal NK/T-cell lymphoma (ENKTCL).Methods:The clinical data of 11 patients with ENKTCL who were admitted to the University of Hong Kong-Shenzhen Hospital from January 2012 to January 2022 were retrospectively analyzed. The patients received 4-6 courses of SIMPLE (cisplatin, gemcitabine, ifosfamide, etoposide, dexamethasone, and pegasparaginase) regimen chemotherapy, and stage Ⅰ and Ⅱ patients who also received local radiotherapy after 2 or 3 courses of chemotherapy. Patients were evaluated for mid-treatment and end-of-treatment outcomes, and the adverse effects of patients were evaluated in each treatment cycle. The Kaplan-Meier method was used to analyze the progression-free survival (PFS) and overall survival (OS) of the 11 patients.Results:All 11 patients were nasal type, with the median age of 41 years old (26-67 years old), including 5 males and 6 females, 3 relapsed cases and 8 newly treated cases. Of the 10 patients evaluated for efficacy, 9 achieved complete remission and 1 achieved at least partial remission (efficacy was assessed based on follow-up). All 11 patients were followed up for a median time of 50 months (15-72 months) and 2 relapsed patients died due to disease progression. The expected 5-year PFS rate and OS rate of 11 patients were both 90.0%, and the expected 5-year OS rate was 100.0% and 66.6% in newly treated and relapsed patients, respectively. Common adverse effects were hematologic adverse reactions, infections, gastrointestinal symptoms, elevated transaminases, and hypofibrinogenemia, all of which were curable. There is no treatment-related death.Conclusions:The SIMPLE regimen for the treatment of ENKTCL has a high remission rate, the patients have long survival time, and the regimen is moderately well tolerated.
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OBJECTIVE@#To explore the genetic etiology and related factors in 1 065 women with spontaneous abortions.@*METHODS@#All patients have presented at the Center of Prenatal Diagnosis of Nanjing Drum Tower Hospital from January 2018 to December 2021. Chorionic villi and fetal skin samples were collected, and the genomic DNA was assayed by chromosomal microarray analysis (CMA). For 10 couples with recurrent spontaneous abortions but normal CMA results for abortive tissues, non-in vitro fertilization-embryo transfer (IVF-ET) pregnancies and no previous history of live births and no structural abnormalities of the uterus, peripheral venous blood samples were collected. Genomic DNA was subjected to trio-whole exome sequencing (trio-WES). Candidate variants were verified by Sanger sequencing and bioinformatics analysis. Multifactorial unconditional logistic regression analysis was carried out to analyze the factors that may affect chromosomal abnormality in spontaneous abortions, such as the age of the couple, number of previous spontaneous abortions, IVF-ET pregnancy and history of live birth. The incidence of chromosomal aneuploidies in spontaneous abortions during the first trimester was compared in young or advanced-aged patients by chi-square test for liner trend.@*RESULTS@#Among the 1 065 spontaneous abortion patients, 570 cases (53.5%) of chromosomal abnormalities were detected in spontaneous abortion tissues, which included 489 cases (45.9%) of chromosomal aneuploidies and 36 cases (3.4%) of pathogenic/likely pathogenic copy number variations (CNVs). Trio-WES results have revealed one homozygote variant and one compound heterozygote variants in two pedigrees, both of which were inherited from the parents. One likely pathogenic variant was detected in the patient from two pedigrees. Multifactorial unconditional Logistic regression analysis suggested that age of patient was an independent risk factor of chromosome abnormalities (OR = 1.122, 95%CI: 1.069-1.177, P < 0.001), the number of previous abortions and IVF-ET pregnancy were independent protective factors for chromosomal abnormalities (OR = 0.791, 0.648; 95%CI: 0.682-0.916, 0.500-0.840; P = 0.002, 0.001), whilst the age of husband and history of live birth were not (P > 0.05). The incidence of aneuploidies in the abortive tissues has decreased with the number of previous spontaneous abortions in young patients (χ² = 18.051, P < 0.001), but was not significantly correlated with the number of previous spontaneous abortions in advanced-aged patients with spontaneous abortions (P > 0.05).@*CONCLUSION@#Chromosomal aneuploidy is the main genetic factor for spontaneous abortion, though CNVs and genetic variants may also underlie its genetic etiology. The age of patients, number of previous abortions and IVF-ET pregnancy are closely associated with chromosome abnormalities in abortive tissues.
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Gravidez , Humanos , Feminino , Idoso , Aborto Espontâneo/genética , Variações do Número de Cópias de DNA , Aberrações Cromossômicas , Transtornos Cromossômicos/genética , Aneuploidia , Aborto Habitual/genéticaRESUMO
OBJECTIVE@#To assess the value of chromosomal microarray analysis (CMA) and trio-whole exome sequencing (trio-WES) for fetuses with increased nuchal translucency (NT) thickness.@*METHODS@#Sixty two pregnant women who had visited Urumqi Maternal and Child Care Health Hospital between June 2018 and June 2020 for NT ≥ 3.0 mm at 11 ~ 13+6 gestational weeks were selected as study subjects. Relevant clinical data were collected. The patients were divided into 3.0 ~ <3.5 mm (n = 33) and ≥3.5 mm groups (n = 29). Chromosome karyotyping analysis and chromosomal microarray analysis were carried out. And trio-WES analysis was performed on 15 samples with NT thickening but negative CMA results. The distribution and incidence of chromosomal abnormalities in the two groups were compared by using chi-square test.@*RESULTS@#The median age of the pregnant women was 29 years old (22 ~ 41 years old), the median thickness of NT was 3.4 mm (3.0 ~ 9.1 mm), and the median gestational age at the detection was 13+4 weeks (11+5 ~ 13+6 weeks). Chromosome karyotyping analysis has detected 12 cases of aneuploidies and 1 case of derivative chromosome. The detection rate was 20.97% (13/62). CMA has detected 12 cases of aneuploidies, 1 case of pathogenic CNV and 5 cases of variant of uncertain significance (VUS), with a detection rate of 29.03% (18/62). The aneuploidy rate for the NT ≥ 3.5 mm group was higher than that for the 3.0 ≤ NT < 3.5 mm group [3.03% (1/33) vs. 41.38% (12/29), χ² = 13.698, P < 0.001]. There was no statistically significant difference between the two groups in the detection rate of fetal pathogenic CNV and VUS (χ² = 0.028, P > 0.05). Trio-WES analysis of 15 samples with negative CMA result and no structural abnormality has identified 6 heterozygous variants, including SOS1: c.3542C>T (p.A1181V) and c.3817C>G (p.L1273V), COL2A1: c.436C>T (p.P146S) and c.3700G>A (p.D1234N), LZTR1: c.1496T>C (p.V499A), and BRAF: c.64G>A (p.D22N), respectively. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG), all of the variants were rated as VUS.@*CONCLUSION@#NT thickening can indicate chromosome abnormality, and CMA and trio-WES may be used for the prenatal diagnosis.
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Gravidez , Humanos , Feminino , Adulto , Lactente , Medição da Translucência Nucal/métodos , Diagnóstico Pré-Natal/métodos , Aberrações Cromossômicas , Aneuploidia , Feto/diagnóstico por imagem , Ultrassonografia Pré-Natal , Variações do Número de Cópias de DNA , Fatores de TranscriçãoRESUMO
OBJECTIVE:To establis h the UPLC fingerprint of Poria co cos aqueous extract ,and to investigate its relationship with sedative and hypnotic effect. METHODS :Ten batches of P. cocos from different areas were extracted with water to obtain the aqueous extract. UPLC method was adopted. The determination was performed on Waters HSS-C 18 column with mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile-methanol (gradient elution ) at the flow rate of 0.4-0.2 mL/min. The detection wavelengths were set at 210 and 242 nm. The column temperature was 40 ℃,and sample size was 2 μL. The fingerprints of 10 batches of P. cocos aqueous extracts were established by using the Similarity Evaluation System of TCM Fingerprint (2012A version),and the common peaks were identified. The sedative and hypnotic effects of 10 batches of P. cocos aqueous extracts from different areas under the synergistic action of pentobarbital sodium were investigated by taking the sleeping rate ,sleep latency and sleep duration of mice as the single efficacy index. After data transformation of single efficacy index and total efficacy (single indexes calculated by analytic hierarchy process ),grey correlation analysis was used to analyze the correlation between the common peaks in fingerprint of P. cocos aqueous extract and the single efficacy index and total efficacy. RESULTS :There were 24 common peaks in 10 batches of aqueous extract of P. cocos , and 11 components were identified , i.e. 16 α-hydroxydehydrotrametenolic acid (peak 6),16α-hydroxytrametendic acid (peak 7),poricoic acid B (peak 9),dehydrotumulosic acid(peak 10),poricoic acid A (peak 12),polyporenic acid C (peak 15),3-O-acetyl-16α-hydroxydehydrotrametenolic acid (peak 17),dehydropachymic acid (peak 20),pachymic acid (peak 21),dehydrotrametenolic acid (peak 22),dehydroeburicoic acid (peak 24). Grey correlation analysis showed ,the correlation between 24 peaks and sleep duration was greater than 0.6(0.611 5- 0.811 8);the correlation between 24 peaks and sleep latency was greater than 0.6(0.605 9-0.790 4),except for peaks 14,24 and 2;the correlation of 24 peaks between sleeping rate was greater than 0.6(0.606 4-0.721 6),except for peaks 23,19,17 and 5; the correlation of 24 peaks between total efficacy was greater than 0.6(0.619 0-0.781 2),except for peaks 2,5,19. The top 10 chromatographic peaks related to the total efficacy were peak 15(polyporenic acid C ),peak 16,peak 8,peak 11,peak 12 (poricoic acid A ), peak 1, peak 7 (16 α-hydroxytrametendicacid), peak 3, peak 9 (poricoic acid B ) and peak 20 (dehydropachymic acid ). CONCLUSIONS :UPLC fingerprint of P. cocos aqueous extract was established and 11 components were identified. Ten components such as polyporus acid C are closely related to the total efficacy of sedation and hypnosis ,which preliminarily reveal the material basis of the sedative and hypnotic effect of P. cocos .
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OBJECTIVE:To study the repa ir,anti-inflammatory and analgesic effects of Compound crocodile oil burn ointment on superficial second-degree burned skin. METHODS :The heated weight was attached to the right depilated skin of guinea pigs for 4 s to induce the model of superficial second-degree burn. After modeling ,guinea pigs were randomly divided into model group , Jingwanhong ointment group (positive control ),formula Ⅰ,Ⅱ and Ⅲ groups of Compound crocodile oil burn ointment (volume fraction 1.5%,3%,4.5%,hereinafter),with 8 guinea pigs in each group. Except for model group ,other groups were smeared with 0.7 g/guinea pigs twice a day for 14 consecutive days. The wound healing was recorded every day ,the healing rate of wound was calculated. HE staining was used to observe the histopathological changes of the wound. The serum levels of EGF ,VEGF, SOD,MDA,TNF-α and IL-1 were detected by ELISA. Eighty Kunming mice were divided into 2 groups,and then sub-grouped into model group ,Jingwanhong ointment group (positive control ),formula Ⅰ and Ⅲ groups of Compound crocodile oil burn ointment,with 10 mice in each group. Then xylene auricle swelling method and acetic acid writhing method were used to investigate the anti-inflammatory and analgesic effects of Compound crocodile oil burn ointment. RESULTS :In the burn repair experiment,after intervention of Compound crocodile oil burn ointment ,the wound area of guinea pigs gradually decreased ,and on the 14th day ,the wound had healed greatly ,and the wound healing rate increased significantly (P<0.01);serum levels of EGF and SOD were increased significantly (P<0.01),while the levels of VEGF ,MDA,TNF-α and IL-1 were decreased significantly(P<0.05 or P<0.01). The thick new epidermal layer was found in wound tissue ,and the connective tissue and neovascularization in the dermis increased significantly. In the anti-inflammatory and analgesic experiment ,after intervention of Compound crocodile oil burn ointment ,the degree of ear swelling and the times of writhing decreased significantly (P<0.05 or P<0.01). CONCLUSIONS :Compound crocodile oil burn ointment shows good skin repair ,anti-inflammatory and analgesic efficacy;the mechanism may be associated with increasing the serum levels of EGF and SOD and reducing the levels of VEGF , MDA,TNF-α,IL-1.
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OBJECTIVE:To study “Qi-invigorating”effect and its possible mechanism of total saponins of Astragalus membranaceus on rats with Qi-deficiency ,and to provide reference for elucidating the material basis of “Qi-invigorating”effect of A. membranaceus . METHODS :Forty male Wistar rats were randomly divided into normal group ,model group ,positive control group [Buzhong yiqi pills ,4.5 g/(kg·d)],A. membranaceus total saponins high-dose and low-dose groups [ 252,28 g/(kg·d),by the amount of total saponins] according to body weight ,with 8 rats in each group. Except for normal group ,the model of Qi-deficiency was made in other groups by the method of “diet disorder+fatigue ”. At the same time ,administration groups were given relevant medicine intragastrically ,and normal group and model group were given constant volume of water,once a day ,for consecutive 21 days. After last administration ,the general situation of rats was observed ;the body weight ,spleen index and thymus index of rats were detected ;weight-bearing swimming time was recorded ;the levels of spleen T lymphocyte subsets CD 3 and CD 4,the levels of ATP and ADP in liver tissue ,serum levels of ALB ,RBC and HBG in blood as well as the serum levels of SOD,MDA,lactate,LDH,CK,IL-2,IL-12 and TNF-α were all detected. RESULTS:Compared with normal group ,body weight,thymus index ,spleen index ,weight-bearing swimming time ,the level of spleen T lymphocyte subsets CD 3,ATP,ADP, ALB,IL-2 and IL- 12 were decreased or shortened significantly in model group (P<0.05 or P<0.01). The levels of MDA , lactate,CK and TNF-α were increased significantly (P< 0.05). Compared with model group ,body weight ,spleen index,weight-bearing swimming time ,the level of spleen T lymphocyte subsets CD 3 and the levels of ATP ,ADP,ALB, RBC and IL- 2 were increased significantly or prolonged(P<0.05);while the levels of MDA ,lactate,CK and TNF-α were decreased significantly in A. membranaceus total saponins high-dose group(P<0.05 or P<0.01).Weight-bearing swimming time ,the levels of ATP ,ADP and IL- 2 in A. membranaceus total saponins low-dose group were increased significantly or prolonged (P<0.05 or P<0.01),while the levels of MDA ,lactate,CK and TNF-α were decreased significantly (P<0.05 or P<0.01). Compared with positive control group ,spleen index ,spleen T lymphocyte subsets CD 3,weight-bearing swimming time and ATP level of A. membranaceus total saponins high-dose group were increased significantly or prolonged (P<0.05 or P<0.01),while MDA levels of A. membranaceus total saponins high-dose and low-dose groups were decreased significantly (P<0.05 or P<0.01). CONCLUSIONS :A. membranaceus total saponins can reduce the body ’s accumulation of blood lactic acid ,the activity of CK ,the level of lipid peroxide and regulate immunity to tonify Qi ,delay fatigue and improve exercise ability.
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OBJECTIVE:Compare the difference of total protein content of Poria cocos from different producing areas. METHODs:Using bovine serum albumin (BSA) as control ,0.4 mol/L sodium hydroxide solution as exctraction solution , Coomassie brilliant blue G- 250 as chromogenic reagent ,visible spectrophotometry at 595 nm was used to determine the contents of total protein of P. cocos ;cluster analysis was used to classify 34 batches(S1-S34)of P. cocos from different producing areas. RESULTS:The linear range of BSA was 1.45-17.40 μ g/mL (r=0.999 6). RSDs of precision ,stability (20 min) and reproducibility tests were all lower than 3%;recoveries were 100.14%-104.26%(RSD=1.43%,n=9). The contents of total protein in 34 batches of P. cocos from different producing areas were 0.388 4%-1.129 7%. The results of cluster analysis showed that among 34 batches of P. cocos ,the total protein content of P. cocos produced in Yingshan county of Hubei province (S2,S3) was higher than 1%,clustered into one category ;the total protein contents of P. cocos produced in Hubei ,Yunnan,Anhui and Hunan(S1,S5-S10,S12,S13,S16,S17,S19-S21,S23-S25,S28,S30,S31)were 0.653 5%-0.946 1%,clustered into one categony,and the remaining batch content were 0.388 4%-0.601 2%,clustered into one category. CONCLUSIONS :Established method is suitable for the content determination of total protein content of P. cocos . The protein content of P. cocos from Yingshan county of Hubei province is the highest ,followed by Yunnan and Anhui in 34 batches of P. cocos from different producing areas.
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Objective: To evaluate periodontal status of 380 pregnant women and its related factors. Methods: 380 healthy pregnant women aged 20 to 43 at 10-35 weeks of gestation were enrolled and received a questionnaire and a periodontal examination, in order to compare and analyse the effects of different ages, gestational weeks and educational levels on periodontal condition. Results: The prevalence of periodontal disease of the subjects was 87. 1%. In first (10-15 weeks), second (16-27 weeks) and third (28-35 weeks) trimester PD (mm) was 2. 48 ± 0. 54, 2. 60 ± 0. 54 and 2. 71 ± 0. 48 respectively (P < 0. 05) ; the percentage of PD≥4 mm in 2 or more sites were 76. 5%, 84. 1% and 93. 5% respectively (P < 0. 05) ; GI and BI did not show significant differences among of the 3 groups trimester stages (P> 0. 05) ; PD in maternal age (year) 20-27, 28-35 and 36-43 years was2. 39 ± 0. 51, 2. 61 ± 0. 53 and 2. 61 ± 0. 56 respectively (P < 0. 05) . A significantly positive linear trend was observed between age and GI (P < 0. 05) or PD (P < 0. 05), but without significant correlation between age and BI. Significant correlation was found between pregnant weeks and PD (P < 0. 05), but without correlation between pregnant weeks and GI (P> 0. 05) or BI (P> 0. 05) .Conclusion: The prevalence of periodontal disease during pregnancy is high. Meanwhile, the periodontal inflammation is increasing worse with the increase of gestational weeks and maternal age.
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Objective To assess the brain development of newborns with cardiac septal defects by MRI.Methods The brain MR images of 150 newborns with cardiac septal defects and 50 normal newborns were analyzed retrospectively.We evaluated the brain development by measuring the four indices of lateral ventricle:anterior horn index (F/F'),body index (D/D'),caudate nucleus index (C/C')and Evans index.Independent samples t test was used to compare the differences between the two groups,and the possible positive diagnostic cut-off points were calculated by using the nonparametric ROC analysis.Results There were no significant differences between the congenital heart disease group and the control group in the two indices:F/F'[(0.301±0.035)vs (0.296±0.031);t=1.035,P>0.05]and Evans index [(0.239±0.052)vs (0.233±0.025);t=0.778,P>0.05].The values of D/D'[(0.261±0.039)vs (0.234±0.032);t=3.873,P<0.05)] and C/C'[(0.138±0.018)vs (0.124±0.015);t=4.479,P<0.05]were significantly higher in the congenital heart disease group than in the control group.In the congenial heart disease group,the area under the ROC curve obtained by D/D'and C/C'were 0.698 and 0.750,respectively.The maximum Yuedeng index corresponding to the D/D'value and the C/C'value were 0.28 and 0.12,respectively. Conclusion The body index(D/D')and the caudate nucleus index(C/C')are sensitive to evaluate the differences of the brain volume between the newborns with cardiac septal defects and the normal newborns.It is helpful to find the abnormal brain volume when the value of D/D'is greater than 0.28 and the value of C/C'is greater than 0.12.
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Objective To investigate the incidence of prozone phenomenon of rapid plasma reagin (RPR) test in syphilis serolog-ic testing and its correlation with the intensity of chemiluminescent microparticle immunoassay (CMIA) results.Methods A total of 101493 patients in our hospital from January 2014 to December 2015 were performed syphilis serologic testing by CMIA ,RPR and treponema pallidum particle agglutination (TPPA).The incidence rate of prozone phenomenon in RPR testing was evaluated.Its in-fluencing factors were investigated by using the Logistic regression.Results Among 101493 serum samples ,2180 cases were posi-tive by CMIA and 767 cases were positive by RPR ,the incidence rate of prozone phenomenon was 3.3% (26/767)in RPR.The Lo-gistic regression results indicated that the incidence of prozone phenomenon was significantly correlated with CMIA S /CO values and RPR titer ,but had no correlation with sex ,age and seasonality.Conclusion Although the incidence of prozone phenomenon is low in syphilis serologic testing ,but it is enough important.The patients with higher S/CO value in CMIA test have a higher inci-dence rate of RPR prozone phenomenon.
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<p><b>OBJECTIVE</b>To explore the application of combined techniques for the prenatal diagnosis of a case with 7q11.23 duplication.</p><p><b>METHODS</b>Amniocentesis was performed in the second trimester for a mother with a high risk suggested by serological prenatal screening. G-banded chromosomal analysis was performed on cultured amniocytes and peripheral blood samples from both parents. DNA from amniotic fluid sample was isolated for a BACs-on-Beads (BoBs) assay. To define the range of duplication, copy number variation was determined with single nucleotide polymorphism array (SNP array, Affymetrix CytoScan 750K) and fluorescence in situ hybridization (FISH) analysis.</p><p><b>RESULTS</b>Chromosomal analysis suggested that the fetus and both parents all had a normal karyotype, while a duplication of 7q11.23 was detected by the BoBs assay. SNP array revealed a 1.5 Mb duplication in chromosome 7q11.23, which was confirmed by FISH.</p><p><b>CONCLUSION</b>Combined prenatal BoBs, SNP array and FISH has enabled effective diagnose of a case with 7q11.23 syndrome.</p>
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Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Gravidez , Bandeamento Cromossômico , Transtornos Cromossômicos , Diagnóstico , Embriologia , Genética , Cromossomos Humanos Par 7 , Genética , Doenças Fetais , Diagnóstico , Genética , Diagnóstico Pré-Natal , Trissomia , GenéticaRESUMO
Objective To investigate the relationship between K+ concentration in rabbit vitreous humor and postmortem interval (PMI) under different ambient temperature. Methods Rabbit corpses were stored at 5℃ , 15℃ , 25℃ , and 35℃ after execution, and 80~100μL vitreous humor was extracted from each eye of the rabbit in turn every 12 hours. The concentrations of K+ were examined by Modular DPPI automatic biochemistry analyzer. The Interpolation Functions were used to analyze the statistical relationship between PMI and K+ concentration under different temperature. Results In each animal group, K+ concentration increased with PMI. Equation was obtained after interpolation analysis on range of temperature 5℃ ~30℃ . The three-variable quintic surface equation was f(x,y)=-1.998e14+1.345e12x+5.902e13y+0.005585x2-4.509e11xy-3.876e12y2-0.0002868x3+0.003545x2y+4.406e10xy2-1.746e10y3+2.669e-6x4-1.568e-5x3y-0.0001771x2y2-1.64e9xy3+6.669e9y4-8.672e-9x5+4.467e-8x4y+2.354e-7x3y2+2.459e-6x2y3+2.05e7xy4-1.214e8y5(R2=0.9956), x stands for temperature, y stands for K+ concentration, f(x,y) stands for PMI. Conclusion The rule of K+ concentration changes at ambient temperature complied with three-variable quintic surface equation distribution. Measurement of interpolation function may be used for PMI estimation at different ambient temperature.
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Testacean traditional Chinese medicine (TTCM), derived from the outer shell of sea or freshwater mollusks, is a special and important category of Chinese medicinal materials. To ensure the effective use of TTCM, a comparative identification study was performed on five commonly-used testacean drugs, including Haliotidis Concha, Arcae Concha, Meretricis Concha, Ostreae Concha and Margaritifera Concha (Shijueming, Walengzi, Geqiao, Muli and Zhenzhumu in Chinese, respectively). Typical morphological photographs of the crude drugs were acquired, and the key microscopic characteristics of the derived powders under normal light microscope and polarized light microscope were summarized. The major results can be concluded as follows: (1) the original species involved in the five TTCMs could be distinguished by their respective interspecies morphological characteristics; (2) the key identification characteristics of the five powdered crude drugs were mainly crystal fragments, with the fragment features under both normal light and polarized light microscope providing powerful points for differentiating the five commonly-used testacean drugs. This study demonstrated that it is feasible to provide authentication for these five kinds of TTCMs by the combination of morphology with microscopy.
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Objective To explore changes of levels of interferon‐γ(IFN‐γ) ,interleukin‐32(IL‐32) and interleukin‐6(IL‐6) in pe‐ripheral blood and the correlation between peripheral IFN‐γ,IL‐32 ,IL‐6 and liver function level and hepatitis B virus(HBV) DNA load in HBV carriers .Methods Sixty HBV carriers ,including 39 cases of chronic HBV carriers and 21 cases of inactive hepatitis B surface antigen(HBsAg) carriers ,and 50 healthy individuals were collected .Serum levels of IFN‐γ,IL‐32 and IL‐6 ,the amount of HBV DNA and liver function were detected ,and clinical correlations were analysed .Results Compared with the control group ,ser‐um levels of IFN‐γ,IL‐32 and IL‐6 of chronic HBV carriers and inactive HBsAg carriers were significantly increased (P0 .05) .There was positive correlation between IL‐32 level and ALT level (r=0 .32 ,P<0 .05) ,and negative correla‐tion between IL‐32 level and ALB level(r= -0 .27 ,P<0 .05) .Conclusion IFN‐γ,IL‐32 and IL‐6 may play important roles in chro‐nic HBV infection ,the levels of IFN‐γ,IL‐32 and IL‐6 could be used as important indicators to assess the severity of inflammation in HBV carriers .
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Objective To investigate the correlation between the liver fibrosis markers laminin(LN),hyaluronan acid(HA),col-lagen Ⅳ(CⅣ)and procollagen type Ⅲ N-terminal peptide(PⅢNP)with the replication of hepatitis B virus(HBV)DNA in the pa-tients with chronic HBV in Lanzhou.Methods Real-time PCR and chemiluminescent immunoassay(CLIA)were used to quantita-tively measure serum HBV-DNA and liver fibrosis markers in 724 cases of HBV infection in Lanzhou with the different clinical classifications and the natural history stage as the background.Results The values of the liver fibrosis indicators and HBV-DNA level in all clinical types were higher than normal levels,and reached to maximum in the liver cirrhosis goup;CⅣ and PⅢNp were highest in the HBeAg (+)CHB group;according to the hepatitis B natural history stage,the PⅢNP and CⅣ values reached the peak value in the immune clearance phase,moreover the CⅣmean value in the HBeAg and HBV-DNA (+)group was higher than that in the HBV-DNA and HBeAg (-)group.4 indicators in the HBeAg (+)CHB group were higher than those in the HBeAg (-)CHB group,but only HA had statistical difference(P <0.01 ).Conclusion There is certain correlation between CⅣ with HBV-DNA and HBV-M,indicating that which can indirectly reflect the degree of liver damage and viral replication level.
RESUMO
Quality control and standardization of traditional Chinese medicines (TCMs) is the key to its globalization. Generally, there is a common practice to select one or more compounds as chemical markers for the purpose of qual-ity assessment. However, how to select chemical markers and how to set the range of effective concentrations are still the bottleneck. The authors proposed a bioactive equivalent combinatorial components (BECCs) as defined labeled amount of active constituents oriented quality control strategy of TCMs based on years of research work. Focusing on the relationship between chemical components efficacy and quality of TCMs, this strategy provides a new perspective and methods to improve quality control of TCMs.