RESUMO
We examined the contractile responsiveness of rat thoracic aortas under pressure overload after long-term suprarenal abdominal aortic coarctation (lt-Srac). Endothelium-dependent angiotensin II (ANG II) type 2 receptor (AT2R)-mediated depression of contractions to ANG II has been reported in short-term (1 week) pressure-overloaded rat aortas. Contractility was evaluated in the aortic rings of rats subjected to lt-Srac or sham surgery (Sham) for 8 weeks. ANG I and II levels and AT2R protein expression in the aortas of lt-Srac and Sham rats were also evaluated. lt-Srac attenuated the contractions of ANG II and phenylephrine in the aortas in an endothelium-independent manner. However, lt-Srac did not influence the transient contractions induced in endothelium-denuded aortic rings by ANG II, phenylephrine, or caffeine in Ca2+-free medium or the subsequent tonic constrictions induced by the addition of Ca2+ in the absence of agonists. Thus, the contractions induced by Ca2+ release from intracellular stores and Ca2+ influx through stored-operated channels were not inhibited in the aortas of lt-Srac rats. Potassium-elicited contractions in endothelium-denuded aortic rings of lt-Srac rats remained unaltered compared with control tissues. Consequently, the contractile depression observed in aortic tissues of lt-Srac rats cannot be explained by direct inhibition of voltage-operated Ca2+ channels. Interestingly, 12-O-tetradecanoylphorbol-13-acetate-induced contractions in endothelium-denuded aortic rings of lt-Srac rats were depressed in the presence but not in the absence of extracellular Ca2+. Neither levels of angiotensins nor of AT2R were modified in the aortas after lt-Srac. The results suggest that, in rat thoracic aortas, lt-Srac selectively inhibited protein kinase C-mediated activation of contraction that is dependent on extracellular Ca2+ entry.
Assuntos
Animais , Masculino , Aorta Torácica/fisiopatologia , Coartação Aórtica/fisiopatologia , Cálcio/metabolismo , Endotélio Vascular/fisiologia , Músculo Liso Vascular/fisiopatologia , Proteína Quinase C/antagonistas & inibidores , Vasoconstrição/fisiologia , Angiotensina I/análise , Angiotensina II/análise , Aorta Torácica/lesões , Aorta Torácica/cirurgia , Western Blotting , Pressão Sanguínea/fisiologia , Cromatografia Líquida de Alta Pressão , Endotélio Vascular/lesões , Músculo Liso Vascular/metabolismo , Fármacos Neuromusculares Despolarizantes/farmacologia , Fenilefrina/farmacologia , Potássio/farmacologia , Proteína Quinase C/metabolismo , Radioimunoensaio , Ratos Wistar , /metabolismo , Vasoconstrição/efeitos dos fármacosRESUMO
We report a male patient who after a fall suffered high cervical spinal cord and head (cerebral) injuries. These injuries led to spinal shock, marked sinus bradycardia and asystolic cardiac and respiratory arrests, recalcitrant central traumatic diabetes insipidus, and death within approximately seven weeks. Temporary transvenous cardiac pacing proved useful in the management of this patient