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Arch. med. res ; 27(1): 67-70, 1996. tab, ilus
Artigo em Inglês | LILACS | ID: lil-200293

RESUMO

The identification of human IgG immunoglobulins on the surface of Vibrio cholerae O1, and Haemophilus influenzae type b microorganisms was assessed via a flow cytometric technique. A group of 31 healthy mother-full term nerwborn duo sera from a non-endemic cholera area was assayed. The sera of mothers and full-term newborns against both microorganisms were compared. The mean fluorescent intensity of the samples was not different at the 0.05 significance level by paired t-test. On the other hand, the immunoglobulins of newborn and mothers for V. cholerae O1 was notably lower when compared with H. influenzae type b microorganisms (p<0.05 by paried t-test, t=-5.570 for mother' sera, and t=-7.496 for the sera of the newborns). These data provide circumstantial evidence that LFC techique would be useful on bacteria-related serology


Assuntos
Humanos , Feminino , Brucella melitensis/isolamento & purificação , Citometria de Fluxo/métodos , Haemophilus influenzae/isolamento & purificação , Imunoglobulina G/imunologia , Imunoglobulinas/isolamento & purificação , Saúde Materno-Infantil , Proteínas da Membrana Bacteriana Externa/isolamento & purificação , Testes Sorológicos , Vibrio cholerae/isolamento & purificação
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