Relation between antioxidants and pollution on experimental animals

The aim of the present work is to study the protective effect of some antioxidants and trace elements against the hazardous effects of carbon tetrachloride [CCI[4]] on hamsters for the possibility of further application on humans. One hundred twenty hamsters weighing 104-128g were divided into 13 groups as follows: 1-Negative Control group fed standard diet, 2-positive control group given carbon tetrachloride CCI[4] only, 3-CC1[4] + zinc, 4 - CCI[4] + beta-carotene,9- CCI[4] + alpha- tocopherol, 6- CCI[4] + selenium. 7- CCI[4] + vitamin C,8- CCI[4] + zinc + beta - carotene, 9- CCI[4] + zinc + vitamin C, 10- CCI[4] + zinc + alpha-tocopheral, 11- CCI[4] + selenium + beta-carotene, 12- CCI[4] + selenium + vitamin C and 13- CCI[4] + selenium + alpha-tocopheral. Carbon tetrachloride [CCI[4] has a direct toxic effect on liver and kidney. Depending on biochemical results, the more antioxidants of liver protection against [CCI[4] toxicity are a tocopherol, followed by selenium, selenium + beta - carotene, vitamin C, zinc + beta - carotene and selenium + alpha tocopherol. The more antioxidant for kidney protection against CCI[4] toxicity is beta -carotene followed by selenium, zinc + beta - carotene, zinc + alpha tocopherol, and zinc + vitamin C. Trace elements should not be given individually especially zinc or selenium as deleterious effects, in spite of its protective effect

Influence of dietary mustard and fennel seeds on hypercholesterolemic, hyperlipidemic rats

This work has been planned to study the effect of dietary mustard [Brassica campestris] and Fennel [Foenicum vulgare Mill] seeds on hypercholesterolemic rats. The male Albino rats with average weight [113 +/- 19.1 g] divided into six groups, group [1] as control [healthy] group; group [2] as high fat cholesterol; groups [3, 4] which rats fed on high fat cholesterol plus 5 or 10% mustard seeds; groups [5. 6] which rats fed on high fat cholesterol plus 5 or 10% fennel seeds respectively. Results obtained revealed that there were highly significant decrease in the mean values of total lipids, total cholesterol, triacylglycerols, VLDL-C, HDL-C, LDL-C and risk ratio. There was highly significant increase in concentration of glutathione [GSH] and superoxide dismutase [SOD] activity while highly significant decrease in malondialdehyde [MDA] as lipid peroxidation. Also results showed highly significant decrease in activities of ALT, AST and ALP and concentration of albumin and creatinine in serum as compared with group [2] fed on high fat cholesterol diet alone [P < 0.01]. It could be concluded that the administration of mustard or fennel seeds at levels of 5 and 10% in high fat cholesterol diet can be able to reduce the lipid profile and lipid peroxidation and maintain kidney and liver in rats