RESUMO
The aim of the present work is to study the protective effect of some antioxidants and trace elements against the hazardous effects of carbon tetrachloride [CCI[4]] on hamsters for the possibility of further application on humans. One hundred twenty hamsters weighing 104-128g were divided into 13 groups as follows: 1-Negative Control group fed standard diet, 2-positive control group given carbon tetrachloride CCI[4] only, 3-CC1[4] + zinc, 4 - CCI[4] + beta-carotene,9- CCI[4] + alpha- tocopherol, 6- CCI[4] + selenium. 7- CCI[4] + vitamin C,8- CCI[4] + zinc + beta - carotene, 9- CCI[4] + zinc + vitamin C, 10- CCI[4] + zinc + alpha-tocopheral, 11- CCI[4] + selenium + beta-carotene, 12- CCI[4] + selenium + vitamin C and 13- CCI[4] + selenium + alpha-tocopheral. Carbon tetrachloride [CCI[4] has a direct toxic effect on liver and kidney. Depending on biochemical results, the more antioxidants of liver protection against [CCI[4] toxicity are a tocopherol, followed by selenium, selenium + beta - carotene, vitamin C, zinc + beta - carotene and selenium + alpha tocopherol. The more antioxidant for kidney protection against CCI[4] toxicity is beta -carotene followed by selenium, zinc + beta - carotene, zinc + alpha tocopherol, and zinc + vitamin C. Trace elements should not be given individually especially zinc or selenium as deleterious effects, in spite of its protective effect
Assuntos
Masculino , Animais de Laboratório , Tetracloreto de Carbono/toxicidade , Fígado/patologia , Cricetinae/sangue , Substâncias Protetoras , Ácido Ascórbico , Selênio , Antioxidantes , Carotenoides , Tocoferóis , ZincoRESUMO
This work has been planned to study the effect of dietary mustard [Brassica campestris] and Fennel [Foenicum vulgare Mill] seeds on hypercholesterolemic rats. The male Albino rats with average weight [113 +/- 19.1 g] divided into six groups, group [1] as control [healthy] group; group [2] as high fat cholesterol; groups [3, 4] which rats fed on high fat cholesterol plus 5 or 10% mustard seeds; groups [5. 6] which rats fed on high fat cholesterol plus 5 or 10% fennel seeds respectively. Results obtained revealed that there were highly significant decrease in the mean values of total lipids, total cholesterol, triacylglycerols, VLDL-C, HDL-C, LDL-C and risk ratio. There was highly significant increase in concentration of glutathione [GSH] and superoxide dismutase [SOD] activity while highly significant decrease in malondialdehyde [MDA] as lipid peroxidation. Also results showed highly significant decrease in activities of ALT, AST and ALP and concentration of albumin and creatinine in serum as compared with group [2] fed on high fat cholesterol diet alone [P < 0.01]. It could be concluded that the administration of mustard or fennel seeds at levels of 5 and 10% in high fat cholesterol diet can be able to reduce the lipid profile and lipid peroxidation and maintain kidney and liver in rats