Antibacterial activity of 3, 3', 4'-Trihydroxyflavone from Justicia wynaadensis against diabetic wound and urinary tract infection

ABSTRACT The present investigation was designed to study the effect of an active compound isolated from Justicia wynaadensis against multi drug resistant organisms (MDRO's) associated with diabetic patients. The drug resistant pathogens implicated in wound and urinary tract infection of diabetic patients were isolated and identified by molecular sequencing. Solvent-solvent fractionation of crude methanol extract produced hexane, chloroform, ethyl acetate and methanol-water fraction, among which chloroform fraction was found to be potent when compared with other three fractions. Further, chloroform fraction was subjected to preparatory HPLC (High-Performance Liquid Chromatography), that produced four sub-fractions; chloroform HPLC fraction 1 (CHF1) through CHF4. Among the sub-fractions, CHF1 inhibited the pathogens effectively in comparison to other three sub-fractions. The purity of CHF1 was found to be >95%. Therefore, CHF1 was further characterized by NMR and FTIR analysis and based on the structure elucidated, the compound was found to be 3,3',4'-Trihydroxyflavone. The effective dose of this bioactive compound ranged from 32 µg/mL to 1.2 mg/mL. Thus, the present study shows that 3,3',4'-Trihydroxyflavone isolated from J. wynaadensis is an interesting biopharmaceutical agent and could be considered as a source of antimicrobial agent for the treatment of various infections and used as a template molecule for future drug development.

Antidiabetic and Antioxidant potential of Andrographis paniculata Nees. leaf ethanol extract in streptozotocin induced diabetic rats.

This study aimed to elucidate the potential of Andrographis paniculata (leaves) both as an antidiabetic and as an antioxidant in streptozotocin induced diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin. After grouping, diabetic rats were administered the leaf ethanol extract (250 and 500 mg/kg b.w.) for 28 days. Fasting blood glucose, body weight, creatinine and urea levels and histopathological study of pancreas were carried out to evaluate the antidiabetic effects. Enzyme activity of superoxide dismutase, catalase and glutathione peroxidase in the liver homogenate was assayed. After the treatment with A. paniculata leaf extract, fasting blood glucose, creatinine and urea levels were found to be decreased in diabetic rats.The extract was found to be non toxic as seen by the normal creatinine and urea levels in the extract fed normal rats. There was an increase in the activity of liver antioxidant enzymes in diabetic treated rats. Histopathological study of pancreas revealed the islet cell restoring and regenerative ability of A. paniculata extract. According to our present findings, A. paniculata leaves possessed significant antihyperglycemic and antioxidant effect in streptozotocin induced diabetic rat which might be due to its islet cell restoring and regenerative ability as well as the upregulation of antioxidant enzymes.