Construction of tissue engineering bone with bone marrow stromal cell sheets / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To construct tissue engineering bone with bone marrow stromal cell (BMSC) sheets of dogs.</p><p><b>METHODS</b>BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis. Allogeneic dogs decalcification bone matrixes (DBM) were prepared. Sixteen dogs were divided into 4 groups. The MSC cell sheets-rhBMP-2-BMSC-DBM were implanted under the left latissimus dorsi myofascial as the experimental side; while the rhBMP-2-BMSC-DBM were implanted in the right side as the control. Ectopic bone formation in vivo was evaluated by histological examination 4, 8, 12, 16 weeks after operation.</p><p><b>RESULTS</b>The osteogenesis in the experimental group was better than that in the control group. New bone area in the experimental side was larger than that in the control group, and the difference was significant (P < 0.05). After 16 weeks, lamellar bone was connected into a film in the experimental group. Haversian system and red bone marrow could be seen.</p><p><b>CONCLUSIONS</b>BMSC cell sheets could promote the bone formation of functional tissue-engineered bone.</p>

99Tcm-MIBI SPECT for the differential diagnosis of parotid tumors / 中华核医学杂志

Objective To evaluate the clinical value of 99Tcm-MIBI SPECT for the differential diagnosis of parotid tumors before treatment.Methods 99Tcm-MIBI SPECT imaging of the parotid region was obtained in 32 patients with parotid tumors before surgery.Early and delayed 99Tcm-MIBI imaging were performed in all patients and the diagnosis was confirmed by pathological results after surgery.The ratio of radioactivity between the tumor and opposite side of normal parotid tissue (T/N) was measured.Fisher exact probability test and t test were used for statistical analysis.Results The sensitivity,specificity and accuracy of 99Tcm-MIBI SPECT imaging for the differential diagnosis of parotid tumor were 90.00％ (9/10),86.36％ (19/22) and 87.50％ (28/32),respectively.Among 22 patients who had benign tumors,19(86.36％) showed negative findings,and the other 3 ( 13.64％ ) patients showed false positive results.In 10 patients with malignant tumors,1 ( 10.00％ ) had false negative findings,and all the other 9 (90.00％)patients showed positive results.The difference between the benign and malignant groups was statistically significant (P =0.00018 ).In the early images,the T/N ratios of benign and malignant parotid tumors were 1.45 ±0.38 and 1.65 ±0.63 (t =20.4,P＜0.01),respectively; and in the delayed images,the ratios were 1.43 ± 0.56 and 1.77 ± 0.59 ( t =2.4,P ＜ 0.05 ),respectively.Conclusion 99Tcm-MIBI SPECT imaging might be useful for the differential diagnosis of parotid tumors before surgery.

Measurement of mandible bone mineral density of adults in north China / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To measure the bone mineral density (BMD) of mandible and investigate the relationship between mandible and the whole body skeleton BMD.</p><p><b>METHODS</b>Healthy volunteers were recruited in north China, which were divided into 6 groups by age: > or = 20, > or = 30, > or = 40, > or = 50, > or = 60 and > or = 70 years older, 10 male and 10 female in each group. Dual-energy X-ray absoptiometery (DXA) was used to measure the BMD of the lumbar spine, the mentum of mandible and the mandibular angle. The results were analyzed statistically.</p><p><b>RESULTS</b>The mineral density (MD) of the mentum was (1.310 9 +/- 0.035 5) g/cm2, the left mandibular angle (1.048 9 +/- 0.013 7) g/cm2, the right mandibular angle (1.0547 +/- 0.014 1) g/cm2, the lumbar spine (L2-L4) (1.121 1 +/- 0.017 2) g/cm2. There was a significant difference in mandibular angle and lumbar MD between men and women (P < 0.05). The MD of mandibular angle and lumbar spine decreased significantly after 50 years of age.</p><p><b>CONCLUSIONS</b>The normal BMD of the mandibular mentum, mandibular angles and lumbar spine is obtained. The BMD of the mandibular angles is closely related to that of the lumbar spine. Mandible can be an appropriate measurement site in the evaluation of skeleton BMD status for the forecast of osteoporosis.</p>

Early laser intervention of hemangioma in facial and neck regions of infant / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The study was to evaluate the method of early laser intervention of hemangioma in facial and neck regions of infant.</p><p><b>METHODS</b>Between January 1999 and December 2006, twelve patients, aged 6 days to 3 months, with cutaneous hemangioma in facial and neck regions, were treated with laser, eight cases with Nd:YAG laser therapy and four cases with Venus laser therapy. Four cases with hemangioma in facial and neck regions of infant treated with oral corticosteroid were as control. The outcome was recorded with 1 to 6 years of follow-up.</p><p><b>RESULTS</b>Total resolution was obtained in twelve patients with laser intervention. Atrophic scars occurred in eight patients with Nd:YAG laser therapy, without other complications, such as ulceration, life-threatening hemorrhage and et al. No scar occurred in four patients with Venus laser therapy. Recurrence was not seen in twelve cases with laser therapy with follow-up. Hemangiomas enlarged continuously in four cases with oral corticosteroid therapy.</p><p><b>CONCLUSION</b>Early laser intervention is an excellent management of cutaneous hemangioma in facial and neck regions of infant.</p>

Study of digital visual mandible / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To build a digital visible model of the mandible in order to provide morphological data for image diagnosis and operation of mandible diseases.</p><p><b>METHODS</b>A male adult cadaver specimen was taken for the purpose of study. A digital human databank was got after fixation, perfusion, refrigeration, embedding, section and photograph of the specimen. The thickness of each layer was 0.1 mm. To select mandible image data from chin to condyle and to draw an outline of mandible and teeth image of each layer in 2D Adobe photoshop 8.0. Finally, reconstruct 3D model of mandible was set up in Amira reconstruction software.</p><p><b>RESULTS</b>A 3D model of mandible was formed accurately using of digital human data. The model was fine, visible and displayed cross-section image of mandible. The anatomical shape of nmandible and mandibular teeth were vividly reappeared in three-dimension. The location and trend of mandibular nerve canal were clearly appeared in the transparent model of mandible. The model could be revolved in 3D.</p><p><b>CONCLUSION</b>The study got a complete and exact cross-section data. The visualization of mandible can be realized in Amira reconstruction software. The reconstituted organs can be recovered to the natural state.</p>

Effect of platelet rich plasma on vascularization of tissue-engineered bone / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the effects of platelet rich plasma (PRP) on vascularization of tissue-engineered bone.</p><p><b>METHODS</b>Bone marrow stromal cell (BMSC) were isolated from iliac bone of dogs. PRP was obtained from the same dog and demineralized bone matrix (DBM) was prepared from homologous bone. Twelve dogs were divided into three groups and the back of each dog was divided into four areas. The DBM- BMSC- PRP was implanted in the area A and B; the DBM-BMSC without PRP was implanted in the area C and D. The implants in the areas A and C were wrapped using myo-fascia with blood vessel of latissimus dorsi. The implants in the area B and D were wrapped using superficial fascia of the back without blood vessel. The implants were taken out 4, 8 and 12 weeks later for examination.</p><p><b>RESULTS</b>The degree of calcification and blood vessel formation of the implants was A > B > C > D.</p><p><b>CONCLUSIONS</b>Both PRP and vessels of latissimus dorsi muscle could promote calcification and vascularization in tissue-engineered bone, when used separately or in combination.</p>

Madelung disease: CT and MRI findings / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the CT and MRI manifestations of Madelung disease.</p><p><b>METHODS</b>The CT and MRI manifestations of 12 cases of Madelung disease were analyzed retrospectively.</p><p><b>RESULTS</b>The CT and MRI manifestations: cross-sectional CT showed that abnormally proliferated fat mainly deposited in anterior or posterior subcutaneous tissues of the neck, deep into the sternomastoid and trapezius muscles, situated in posterior cervical triangle, around the salivary glands, and excess fat was also found to be situated in supraclavicular fossa, around the paraspinal muscles and larynx, and so on. The abnormally proliferated fat can compress surrounding organs and tissues. The density of the abnormally proliferated fat was equal to that of normal fat. The MRI manifestations: the distribution of the thickened fat was equal to that seen with CT. The signal intensity of the thickened fat was equal to that of normal fat.</p><p><b>CONCLUSIONS</b>CT and MRI can accurately show distribution of the thickened fat, compression of surrounding organs and tissues and exclude soft tissue tumors. MRI seemed to be superior to CT.</p>

Establishment and identification of biological characteristics of rat monoclone cell line Rca-B / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To establish a monoclone cell line of squamous cell carcinoma (SCC) in rat buccal mucosa and to study its biological characteristics.</p><p><b>METHODS</b>SCC in rat oral mucosa was induced by adding 4-nitroquinoline-1-oxide (4NQO) into the SD rats' drinking water, and the cancer cells were then cultured to obtain mixed cells in vitro. The mixed tumor cells were purified by mono cell cloning method. The biological characteristics of the cells were studied by microscope and electronic microscope observation, chromosome analysis, Methyl thiazolyl tetrazolium (MTT) test, flow cytometry assay and immunohistochemistry staining. Hypodermic inoculations of the cells in nude mice and injection of the cells by nude mice tail veins were performed to observe the tumor formation and long distance metastasis.</p><p><b>RESULTS</b>The morphology proved that the cell line was squamous cell carcinoma cells, which were cultured from one cell. The population doubling time for passage 65 cells was 25.44 hours. The cells in S-phase accounted for 20.13% of the cell cycle. The chromosome modal number was 84. All the cells expressed the proteins of cytokeratin and vimentin. The xenograft rate and the tumor metastatic rate to the lung were 100% in nu/nu BALB/C mice, but the homograft rate was zero in SD Rats.</p><p><b>CONCLUSIONS</b>Rca-B was a typical oral squamous cell carcinoma cell line derived from Sprague-Dawley rat buccal mucosa carcinoma, and the cell line has high metastatic potential and its biological characteristics were well ascertained.</p>

Early treatment of middle ear disease in cleft palate infants / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To explore the effect of myringotomy with insertion of tube and tympanocentesis on alleviating secretory otitis media (SOM) and hearing loss in cleft palate infants.</p><p><b>METHODS</b>Nineteen cleft lip and palate infants with SOM and hearing loss (38 ears) were treated with myringotomy with insertion of ventilation tube at the same time of repair of the cleft lip, who were performed averagely at 6.9 months of age. Fifteen cleft lip and palate infants with SOM (30 ears) were treated with tympanocentesis at the same time of repair of the cleft lip averagely at 6.6 months of age. All cases were followed up from 1 week to 12 months and estimated by auditory brainstem response (ABR) and acoustic immitance audiometry.</p><p><b>RESULTS</b>The average wave V reacting thresholds of ABR were separately 55.41 dBnHL and 28.48 dBnHL, and 79.17% tympanogram B changed to tympanogram A in cleft palate infants with insertion of tube before and after operation. The average wave V reacting thresholds of ABR were separately 40.63 dBnHL and 26.50 dBnHL, and 40.91% tympanogram B changed to tympanogram A in cleft palate infants with tympanocentesis preoperatively and in 1 week postoperatively, in whom the average hearing thresholds and tympanograms had no significant difference preoperatively and in 1 or 3 months postoperatively (P>0.05).</p><p><b>CONCLUSION</b>The early myringotomy with insertion of tube is effective to restore the function of the middle ear in cleft palate infants with SOM, so to suggest to be performed at the same time of the repair of cleft lip within the first 1-year of life. The tympanocentesis should not be used as a regular management in the cleft palate infants with SOM.</p>

Effect of platelet-rich plasma and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of platelet-rich plasma (PRP) and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs.</p><p><b>METHODS</b>Bone marrow stromal cells (BMSCs) were isolated from iliac bone of dogs. PRP was obtained from the same dog. And demineralized bone matrix (DBM) were prepared from homologuous bone. ABCD 4 areas were divided on the back of dog. PRP/BMSCs/DBM was implanted around the vessels of lattisimus dorsi muscle in the A. PRP/BMSCs/DBM wrapped by superficial fascia in the B. BMSCs/DBM was implanted around vessels of lattisimus dorsi muscle in the C. BMSCs/DBM wrapped by superficial fascia in the D area of the same dog. 4, 8, 12 weeks after implantation, gross specimen and histology examination were made.</p><p><b>RESULTS</b>Osteogenesis and blood vessel formation results were A>B>C>D area.</p><p><b>CONCLUSION</b>The results suggested that the PRP and latissimus dorsi myofascia with blood vessels could promote calcification and vascularization in tissue-engineered bone.</p>

Applied anatomy of adult mandibular ramus / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study applied anatomy of adult mandibular ramus and condyle.</p><p><b>METHODS</b>Observation and measurement were made on 16 adult mandibular rami and condyles.</p><p><b>RESULTS</b>Thickness became gradually greater within 15 mm from the back edge of ramus. Some adult mandibular rami had no spongy bone at the level of 10 mm above mandibular foramen.</p><p><b>CONCLUSIONS</b>These date are very important for application of lag screw osteosynthesis in condylar fracture.</p>

Maxillary sinus lifting and simultaneously placement of implants from the top of alveoli / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of maxillary sinus floor elevation by the Frialit-2 Bone Condenser for implantation.</p><p><b>METHODS</b>11 patients underwent sinus floor lift by The Frialit-2 Bone Condenser and were inserted 14 implants. The time of following up was 10 - 21 months.</p><p><b>RESULTS</b>There were no implant loose or lost, no clinical complaint of maxillary sinus area, and X-ray exam showed well osseointegration.</p><p><b>CONCLUSIONS</b>The Frialit-2 bone condenser can be used for lifting sinus floor, and the sinus elevation without lateral access allows the insertion of implants with no additional surgical stress for the patients.</p>

Comparative study on computed orthopantomography and film radiographic techniques in the radiography of temporomandibular joint / 中华放射学杂志

Objective To compare the computed orthopantomography(COPT)with Sh?ller radiography(SR),film orthopantomography(FOPT)and other traditional radiographic techniques in the radiography of temporomandibular joint(TMJ).Methods Ninty-eight cases were randomly divided into 3 groups,and the open and close positions of TMJs of both sides were examined with SR,FOPT,and COPT, respectively.The satisfactory rates of the X-ray pictures were statistically analyzed with Pearson chi-square in SPSS10.0,and the satisfactory rates were analyzed with q test between the groups.Results One hundred and forty-four of the open and close positions of 144 TMJ pictures of the COPT group,128 of 128 of the FOPT group,and 6 of 120 of the SR group were satisfactory in the mandible ramus of the TMJ,with satisfactory rate being 100%,100%,and 5%,respectively(P0.01),respectively between FOPT and COPT groups.The difference was not statistically significant.The exposure was as follows:COPT,99—113 mAs;FOPT,210—225 mAs;and SR,48—75 mAs.Therefore,COPT and FOPT were superior to SR in the pictures of the mandible ramus,coronoid process,and incisure,but inferior in the joint space pictures.The satisfactory rates of the condylar process and articular tubercle were same in the 3 groups.The exposure of the FOPT group was greater than that of the COPT and SR groups.Conclusion COPT is superior to SR and FOPT in TMJ radiography,and should be applied widely in the clinic.