Symptomatic characteristics of chronic rhinosinusitis patients and symptom-specific outcomes after endoscopic sinus surgery / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the symptomatic characteristics of chronic rhinosinusitis patients and the report symptom-based outcomes after endoscopic sinus surgery.@*METHOD@#One hundred and nineteen chronic rhinosinusitis patients underwent endoscopic sinus surgery, including 52 patients of chronic rhinosinusitis without nasal polyps and 67 patients of chronic rhinosinusitis with nasal polyps, were enrolled. Patients were asked to evaluate their symptoms before surgery and 12 months after endoscopic sinus surgery using 10 cm visual analog scale measures.@*RESULT@#The most commonly reported symptoms were nasal obstruction, nasal discharge, headache, facial pressure and altered sense of smell. Compared with patients of chronic rhinosinusitis with nasal polyps, patients of chronic rhinosinusitis without nasal polyps reported significantly higher scores of nasal discharge, whereas lower scores of altered sense of smell (P<0.01 for both). The most disturbing symptom was nasal discharge and altered sense of smell for chronic rhinosinusitis patients without and with nasal polyps, respectively. After endoscopic sinus surgery, the scores for all studied symptoms were improved greatly in both chronic rhinosinusitis with and without nasal polyps groups (P<0.01 for all).@*CONCLUSION@#Chronic rhinosinusitis patients with and without nasal polyps present different symptomatic characteristics. Endoscopic sinus surgery can improve patient-based symptoms significantly. Visual analog scale is a simple and powerful tool to evaluate chronic rhinosinusitis symptoms.

Detection of Segregation of Foreign Genes in Transgenic Wheat Lines by Multiplex Polymerase Chain Reaction / 中国生物工程杂志

The method of multiplex PCR was set up to identify two or three transgenes in one reaction such as uidA and bar; uidA and IDx5 or uidA, bar and 1Dx5 genes. Three sets of primer pairs which was specific to each of these three genes respectively were designed and synthesized. Recombinant plasmids pAHC25 and p1Dx5 harboring uidA + bar and 1Dx5 gene separately were used as template DNA in the process of optimizing an multiplex PCR reaction. The optimal annealing temperature for uidA and bar MPCR is range from 57. 1℃-62. 3℃ , for uidA and 1Dx5 is range from 60℃ to 60. 6℃ , and for uidA、bar and 1Dx5 range from 57. 0℃-58. 4℃. The amount of template for MPCR is twice as much as that for simplex PCR, while the concentration of primers is the same with simplex one. Less than 50bp MPCR products can be separated clearly by 10% non-denaturalized polyacrylamid gel electrophoresis. Fourteen transgenic wheat lines were tested by multiplex and simplex PCR respectively, which shows the same results and hence presents that MPCR is the reliable, rapid and high-effective approach to detect foreign genes from transgenic plant.