Preparation and dissolution rate determination of oridonin solid dispersion / 医学研究生学报

Objective Oridonin is a poorly water soluble drug with low bioavailability.The study aimed to prepared improve the dissolution rate of oridonin by preparation of oridonin solid dispersion with poloxamer. Methods The oridonin solid dispersion was prepared by dissolvent method with poloxamer188 as the carrier and determined by DSC and powder X-ray diffraction.The dissolu-tion rates in different pH dissolution mediums of pure drug, physical mixture and solid dispersion were determined by HPLC method. Results Oridonin exists as an amorphous state in the solid dispersion.Compared with the pure drug and physical mixture, the orido-nin solid dispersion was improved greatly without the pH influence on the drug release.The dissolution rate of oridonin solid dispersion in water, pH 6.8 PBS and pH 1.2 HCl were (92.6 ±4.2)%, (93.1 ±3.5)%and (94.4 ±2.9)% respectively. Conclusion Taking inpoloxamer188 as the carrier, the oridonin solid dispersion has successfully developed its dissolution rate.

Manifestations of contrast-enhanced ultrasound in different stage cervical cancer / 中华超声影像学杂志

Objective To summarize the manifestations of contrast-enhanced ultrasound in different stage cervical cancer.Methods Contrast-enhanced ultrasound using pulse inversion harmonic imaging and on time-intensity curve was undergone in 48 cases with pathologically diagnosed cervical cancer, data were analyzed to summarize the features of contrast enhancement.Results Lesion tissue was enhanced homogeneously or heterogeneously earlier than myometrium.Lesion exhibited earlier washout than that of myomerium in the later phase, while the peripheral area still remained hyper-enhancement.Lesion invasion and borderline could be determined in ultrasound clearly.Conclusions Contrast-enhanced ultrasound could accurately diagnose the stage of cervical cancer and lesion invasion.It may compensate for the shortcomings of conventional ultrasound in the diagnosis of cervical cancers and disease stage.

β-tricalcium Phosphate Seeded with Bone Marrow Mesenchymal Stem Cells as Bone Graft Substitute for Posterolateral Spinal Fusion / 中国康复理论与实践

@# ObjectiveTo evaluate whether β-tricalcium phosphate (β-TCP) combined with bone marrow mesenchymal stem cells (BMSCs) can be used for lumbar posterolateral spine fusion (PLF) instead of autogenous bone graft. Methods6 crab-eating macaques underwent bilateral PLF at L4-5, and divided into 3 groups that implanted β-TCP/BMSCs composite, autogenous bone, and β-TCP. Monkeys were sacrificed 12 weeks after implantation. Manual palpation, micro computed tomography, peripheral quantitative computed tomography (pQCT), and histology were used to assess bone formation. ResultsManual palpation showed that 75% of β-TCP/BMSCs composite group and autogenous group achieved solid spine fusion, whereas none of β-TCP group fused. Histological analysis showed that all of the β-TCP/BMSCs group achieved massive bone formation. Bone mineral density (BMD) evaluated with pQCT in the β-TCP/BMSCs group increased by additional new bone. Conclusionβ-TCP/BMSCs composite can be used for PLF instead of autogenous bone graft.

Application of parvule-impacted bone graft in the posterior lumbar interbody fusion / 中国矫形外科杂志

[Objective] To investigate the feasibility of posterior interbody parvule-impacted bone graft fusion.[Method] A retrospective study was performed in 31 patients admited to our hospital in 2003~2006(M=14,F=17).The ages were 55 to 80,with an average of 66.2 years.There were 9 cases of spondylolisthesis,6 of degenerative unstability and 16 of developmental stenosis.All patients underwent posterior interbody impact bone graft fusion and pedicle screw fixation.The pre-and post-operative JOA score were used to calculate the improvement rate.The postoperative bending-extending plain films were taken to observe whether the fusion was successful.[Result]All patients were followed up for 12~18 months.Three patient had leg symptoms at postoperation and were released 3 months later.Thirteen patients had fusion at 6 month,16 had union at 12 month,and 2 had union at 18 month.The lumbar lordosis angle and intervertebral height got significant restoration.The spinal fusion rate was 100%.No dura tear leakage of cerebrospinal fluid or wound infection was found.No graft absorption,displacement or collapse occurred.There were 16 cases excellent,12 cases good,and 3 cases fair.JOA score was 24~29(average 27.4?1.9)at postoperation.The improvement rate was 89.4%.[Conclusion] Posterior interbody parvule-impacted bone graft is a feasible fusion method.The method is simple and cheap,with advantages of less injury and high fusion rate.

Determination of Berberine in Sanhuang Tablets / 中成药

Objective: To establish a method for the determination of berberine in Sanhuang Tablets. Methods: By means of colorimetry the complex was determined by combining picric acid and berberine hydrochloride. Results: The method is reproducible. The average recovery was 103.3%. Conclusion: The method can be used for content determination of berberine in Sanhuang Tablets.