Laparoscopically assisted neovaginaplasty using peritoneum as vaginal mucous substitute / 中华整形外科杂志

<p><b>OBJECTIVE</b>To practice a more atraumatic, physiological and aesthetically valued approach of construction for neovagina.</p><p><b>METHODS</b>Laparoscopically using peritoneum as neovagina lining.</p><p><b>RESULTS</b>From March 2005 to September 2006, this technique was adopted to treat 10 patients whose diagnosis was congenital absence of vagina. The ages of the patients were from 19 to 32. The operation lasted average 2.34 hours. And hospitalization was about 20.5 days. Follow-up ranged from 3 - 12 months. No complication occurred. All of the patients was satisfied with their sexual life.</p><p><b>CONCLUSIONS</b>Laparoscopically assisted neovaginaplasty, in which peritoneum was substituted for vaginal mucous membrane, was a kind of ideal approach of vaginal creation.</p>

Effects of the site and method of liposuction on differentiation of human preadipocytes / 中华整形外科杂志

<p><b>OBJECTIVE</b>To illustrate the effects of the site and method of liposuction on differentiation of human preadipocytes.</p><p><b>METHODS</b>Forty-two fatty samples were obtained with liposuction, which were then divided into four groups according to operation sites (abdomen, hip or extremity) and the methods (conventional negative-pressure or syringe method). Each sample was treated with collagenase I to release preadipocytes for in vitro culture. Affected by the differentiation-induced agents for 9 days or 28 days, the cultured adipocytes were stained with Nile red and observed under a fluorescent microscope. The differentiation rates were examined with flow cytometric analysis and the quantity of intracytoplasmic lipids was determined with oil red O staining. The results were analyzed with independent samples t-test (Mann-Whitney) using SPSS 10.0.</p><p><b>RESULTS</b>There was no statistical difference in differentiation at the 9th day or 28th day among the preadipocytes obtained from the abdomen, hip or extremity with the negative-pressure method. The preadipocytes from the abdomen differentiated more at the 28th day than the 9th day (P < 0.05), which was not observed in the hip or the extremity. The preadipocytes obtained from the abdomen with the negative-pressure method differentiated more than those with the syringe method (P < 0.05).</p><p><b>CONCLUSION</b>No essential difference was found in preadipocyte differentiation among the liposuction sites, while the abdomen might have some superiority. The negative-pressure method of liposuction is the first option in future research of tissue engineering. The flow cytometric analysis is a convenient way to study preadipocyte differentiation.</p>

Mandibular contour sculpture by osteotomy / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the method for mandible osteotomy in order to make the mandible of various square face appear harmony.</p><p><b>METHODS</b>According to different types of the mandible, mandible angle osteotomy was performed in combination with mandible edge, mandible half ring osteotomy or chin augmentation.</p><p><b>RESULTS</b>A total of 312 cases have been treated since 1996. In this series, mandible angle and mandible edge osteotomy was performed in 200 cases; only mandible edge osteotomy in 23; mandible half ring osteotomy in 15, chin sharpening in 9, chin augmentation with autogenous bone implantation, in 32. Postoperative follow-up of 150 cases for 1-12 months showed that the satisfactory rate was 97%.</p><p><b>CONCLUSION</b>Integrated mandible osteotomy can make the square face look natural and nice-looking.</p>

Leg re-contouring by using a new technique of partially removing gastrocnemius / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate a method to partially remove gastrocnemius for improvement of the contour of the leg.</p><p><b>METHODS</b>Thirteen patients with bulked calf were undergoing the treatment. With a self-designed apparatus, the gastrocnemius was partially removed by placing the device in the muscle through an incision in the popliteal fossa. The follow-ups were carried out for 1-6 months.</p><p><b>RESULTS</b>Thirteen patients were successfully treated by above mentioned technique. The removed amount of muscle was weighted between 11 g and 201 g, averaged 77 g. All of the patients could be able to walk three days after the operation. But, the normal walk had to take 1 month after of the surgery for recovering. The function of the ankle joint was not obviously influenced.</p><p><b>CONCLUSION</b>The above mentioned technique is a safe and effective method for re-contouring the leg.</p>