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Artigo em Chinês | WPRIM | ID: wpr-263891

RESUMO

<p><b>OBJECTIVE</b>Current study was conducted to investigate and compare the impact of temperature and pH on the activities of amylase, protease and lipase in alimentary tract of Whitmania pigra.</p><p><b>METHOD</b>The responses of amylase, protease, and lipase activities were determined over a wide range of temperatures (7-52 degrees C) and pH gradient (2.2-11.2).</p><p><b>RESULT</b>The highest lipase activity was found under 37 degrees C, pH 8.2, and the highest amylase activity was detected under 37 degrees C, pH 5.2, while protease activity peaked at 42 degrees C, pH 3.2 or pH 9.2.</p><p><b>CONCLUSION</b>The optimal temperature in alimentary tract of Wh. pigra for lipase and amylase was 37 degrees C, and the responding temperature for protease was 42 degrees C. The optimal pH value in alimentary tract of Wh. pigra for lipase and amylase was pH 8.2 and pH 5.2, respectively. While pH 3.2 or 9.2 seems to be both favorable for high protease activity.</p>


Assuntos
Animais , Amilases , Química , Metabolismo , Sistema Digestório , Química , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Sanguessugas , Química , Lipase , Química , Metabolismo , Peptídeo Hidrolases , Química , Metabolismo , Temperatura
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