Hygiene risks and management advices for "Room Escape" venues / 环境与职业医学

In recent years, the "Room Escape" industry in China is booming, and this real live game has become popular. However, health threatening events such as fire, trauma, coma, and lung infection in the game venues have been reported at home and abroad. If these venues are not supervised in time, there will be great potential public safety and health hazards. In this review, we summarized the hidden dangers of public safety and health in such venues, including fire and safety hazards, persistent stress state, indoor ventilation problems, risk of contagious infection, non-player characters’ health risk, special film effects of 4D cinemas, mosquito problems, light pollution and noise, and food safety. Meanwhile, health management recommendations for the game venues were proposed from the aspects of formulating health management standards, improving health security measures, and strengthening related health education to players, so as to improve the hygiene levels of such venues and protect players and staff from injuries or diseases.

Intervention effect assessment of response to heatwave in communities of four cities, China / 中华预防医学杂志

Objective@#To evaluate the intervention effects of response to heatwave in communities of four cities, China.@*Methods@#Baseline survey on heatwave and climate change related knowledge, attitude and practice (KAP) was conducted in the pilot communities in Harbin, Nanjing, Shenzhen and Chongqing, using face-to-face questionnaire interview in November, 2011 to November, 2013. Finally, 1 604 residents were interviewed. Intervention measures were implemented in summers of 2013 and 2014, including delivering early warning information of heatwave health risk and launching health education and promotion. The second survey was conducted in same communities using the same questionnaire and sampling method as baseline survey in November, 2014, and 1 640 residents were interviewed. The Chi-square test was used to compare the demographic characteristics and KAP of community residents between before and after intervention, and the factors that affected the intervention effect were selected by logistic multiple stepwise regression model.@*Results@#The age of the residents interviewed before and after intervention was (46.4 ± 15.5) years and (45.0 ± 15.9) years, respectively. Overall, the residents' awareness rates of heatwave before and after intervention were 70.5% (1 131/1 604) and 82.9% (1 359/1 640) (χ2=69.40, P<0.001). The rate of residents who had wished to receive early warning information increased 6.3% (χ2=41.11, P<0.001), which reached 94.6% (1 551/1 604) after intervention from 88.3% (1 416/1 604) in baseline survey. Both heatwave health risk early warning and health education had big impacts to residents. There were 92.7% (1 105 residents) among the 1 192 residents who had received the early warning information arrange work and rest time according to the early warning information and 93.0% (1 231 residents) among the 1 323 residents who knew about health education activities being conducted in community thought that the community health education activities had made active role in protecting health from heatwaves. After a series of intervention, male had a effect on attitude about hot wave than female in Nanjing and Chongqing, OR (95%CI) were 1.48(1.02-2.16) and 1.45 (1.18-2.05) , respectively; compared with subjects below primary school education, people with college degree or above had higer KAP in all cities (ORs range from 1.18 to 2.05), P<0.05; regular physical exercise (ORs range from 1.39 to 2.70) also had profound impacts on KAP in all cities (P<0.05).@*Conclusion@#s Early warning and health education were effective measures to enhance residents' response capacity to climate change.

Simultaneous isolation of myocardial cells and cardiac fibroblasts from neonatal rats / 中国组织工程研究

10.3969/j.issn.2095-4344.2013.24.007

Study on the causes of deafness in a consanguineous marriage family using DNA microarray / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To determine the possible gene mutation and its different sites that leads to hearing loss in a consanguineous marriage pedigree.@*METHOD@#Blood samples from a Changchun pedigree were obtained with informed consent. Their genomic DNA were extracted from peripheral blood and PCR was performed. Nine of hot spot mutations in four most common deafness pathologic gene were detected with the DNA microarray, including GJB2, GJB3, PDS and mtDNA 12S RNA gene. At the same time, the results were confirmed with the traditional methods of sequencing.@*RESULT@#GJB2 gene of 235 delC and 299-300 delAT compound heterozygous mutation was found in propositus. His father was 299-300 delAT homozygous mutation and mother was 235 delC homozygous mutation. In the relatives on the paternal side, the affected patients all were 299-300 delAT homozygous mutation and normal hearing member was 299-300 delAT heterozygous carrier. This GJB2 mutation come from grandparents of consanguineous marriage.@*CONCLUSION@#GJB2 gene mutation played on an important role in this deafness family. It is essential approach for genetic diagnosis of non - syndromic sensorineural hearing loss.

Effect of glial cell-derived neurotrophic factor-modified adipose-derived stem cells on survival of co-cultured dopaminergic neurons / 中国组织工程研究

BACKGROUND: Reports regarding adipose-derived stern cells (ADSCs) differentiation into dopaminergic (DN) neurons are few in addition, there is not experimental evidence of the effect of ADSCs on maintaining the survival of DN neurons.OBJECTIVE: To investigate the effect of glial cell-derived neurokophic factor (GDNF)-modified adipose-derived stem cells on survival of DN neurons under co-cultured condition.DESIGN, TIME AND SETrlNG: The in vitro cytology experiment was conducted at the Institute of Otolaryngology-Head and Neck Surgery and Key Laboratory of Zoonoses of Ministry of Education between March and December 2007.MATERIALS: Wistar rats with 3-weeks-old, or 14 days of pregnancy were provided by Norman Bethune College of Medicine, Jilin University.METHODS: The GDNF recombinant adenovirus was constructed by using pAdTrackCMV and pAdEasy-1 system. DN neurons were obtained from the rostral mesencaphalic tegmentum of Wistar rat embryos by using trypsin and collagenase method. ADSCs isolated from rat inguinal fat pads were digested with collagenase Ⅱ, cultured and passaged in vitro. When the cells reached 60% cenfluency at the 3rd passage, cells were transfectad with 1×109vp/mL of Ad-GDNF for 1 hour and then transferred into growth medium for another 24 hours, and GDNF level in cell supematant was detected by ELISA assay. Meanwhile, the co-cultured of ADSCs and DN neurons were carried out for following 7 days. With GFP-modified ADSCs was served as a control group.MAIN OUTCOME MEASURES: The effect of co-cultured condition on the survival of DN neurons, as well as the differentiation of GDNF-modified ADSCs was detected by immunofluorescence staining.RESULTS: GDNF appeared in ADSCs supematant at 24 hours after Ad-GDNF transfection and reached a peak at 72 hours.There was approximately 80% GFP-positive labeled in ADSCs. The tyrosinase hydroxylase staining results demonstrated that the rate of survival DN neurons were significantly increased than in DA neurons cultured alone, co-cultured group of GFP-modified ADSCs and GDNF-modified ADSCs groups (55%, 15%, 25%, P < 0.01). However, there were no co-expressing TH and GFP positive cells appeared at 7 days of co-culture, which indicated that the co-cultured condition was not available to ADSCs differentiation.CONCLUSION: The co-cultured of GDNF modified ADSCs and DN neurons can promote the survival and growth of cultured DN neurons, however, it can not induce ADSCs differentiate into DN neurons.

Trend of Meteorological Variables in Shenzhen from 2000 to 2005 / 环境与健康杂志

0.05). The percents of high to 35 ℃ and of low to 10 ℃ of day average temperatures did not possess significance. There were significant differences among the temperatures in spring, summer and autumn. As for the relative humidity and rainfall amount from 2000 to 2005, those in 2000 were the maximum and the former was significant(P

Quality Analysis of Finished Water of Municipal Centralized Water Supply System in Shenzhen / 环境与健康杂志

Objective To investigate the water quality of finished water of the centralized water supply system in Shenzhen according to 106 indexes of water quality standards of China.Methods The collection and preservation of water samples was according to the standard examination methods for drinking water-collection and preservation of water samples (GB/T 5750.2-2006).The sanitary quality of the finished water samples collected from 35 centralized water supply systems in cities were determined and evaluated according to the Standards for Drinking Water Quality (GB 5749-2006) in May of 2008.Results The average qualified rate of drinking water was 82.9% (29/35) in Shenzhen.Among 106 indexes,4 indexes (such as turbidity,aluminum,manganese and free chlorine residue) exceeded the standard limits in degrees in some centralized water supply system.Conclusion According to the results of the present paper,it is considered that the water quality of the product water from the centralized water supply system in Shenzhen is good.

Genotoxicity of Organic Extracts from Product Water of Water Plants and the Influencing Factors / 环境与健康杂志

A(D、B were negative); As for TA100(+S9), only E was positive. Conclusion The mutagenicity of product water of six water works is relatively high,the type of mutagenicity is mainly code-shifting. Taking river water as the water source,using chlorine dioxide and active carbon filtration and no using pretreatment of chlorination may reduce the mutagenic organics.

Pollution Status of Giardia lamblia and Cryptosporidium parvum in the Centralized Water Supply Enterprises,Shenzhen,2008 / 环境与健康杂志

Objective To investigate water treatment technology and pollution status of Giardia lamblia and Cryptosporidium parvum in resource water and finished water of the centralized water supply enterprises in Shenzhen,and to provide scientific basis for waterborn infectious disease control.Methods Field hygiene assessment were conducted on the resource water and water treatment technology of 19 water supply enterprises during May to July,2008.One water sample was collected from resource water and finished water in each centralized water supply enterprise respectively,and was detected for Giardia lamblia and Cryptosporidium parvum.Results The routine traditional water treatment technology including coagulation deposition,quartz sand filtration and chlorine disinfection was applied in all of the 19 centralized water supply enterprises.Giardia lamblia and Cryptosporidium parvum were not found in samples from the source water and finished water.Conclusion The centralized water supply system in Shenzhen isn't polluted by Giardia lamblia and Cryptosporidium parvum.

Air Pollutants Change in Shenzhen 2002-2007 / 环境与健康杂志

Objective To analyze time-space variation of air pollutants(SO2,NO2,PM10) and the correlation among them in inner region and outer region of Shenzhen from 2002 to 2007.Methods The daily concentration means of SO2,NO2 and PM10 during 2002 to 2007 from the nine environmental monitoring sites in Shenzhen were collected.The determination was conducted according to the Ambient Air Quality Criteria.Results From January to December,variation of air concentration of SO2,NO2 and PM10 met the "V" shape in inner region and outer region of Shenzhen in 2002-2007,which was descending from January to June and ascending from June to December.The level of the air pollutants in inner region and outer region was higher in fall and lower in summer.The air pollution level of the inner region was increasing from 2002 to 2004,then decreased in 2005,but increased again in 2006 and 2007.The tendency of the outer region was similar with the tendency of the inner region from 2002 to 2006,but the level of pollutants decreased in 2007.The air concentration of SO2 in outer region was higher than that in inner region in 2002-2007.The air concentration of NO2 and PM10 in outer region was higher than that in inner region in 2002 to 2006,but lower in 2007.The air concentration of SO2 in inner region and outer region in 2002-2007 was under the limit of grade 2 according to the GB 3095-1996,near to grade 1 in inner region.The air concentration of NO2 and PM10 was under the limit of grade 3 and grade 2 respectively.There was a close correlation among the levels of SO2,NO2,PM10 in Shenzhen.Conclusion The air quality in Shenzhen is better in China.The time-space variation of the air pollutants is obvious and a close correlation exists among the air pollutants.

Study on Mutagenecity of Indoor Decoration Materials / 环境与健康杂志

ve To study the mutagenecity of volatile organic compounds (VOCs) emitted from decoration materials to experimental animals and human subjects so as to provide some technical basis for the management of safety and hygienic quality of indoor decoration materials. Methods The micronucleus test was carried out in mice exposed to the tested toxicants in an experimental cabinet, in which the tested VOCs were prepared at the various concentrations which were 5, 10, 20 and 40 times as high as the concentrations of VOCs in decorated rooms monitored on site respectively. The frequencies of micronucleus of peripheral blood lymphocytes were examined among waitress working in decorated indoor environment. Results The concentrations of formaldehyde, benzene, methylbenzene, ethyl acetate, butyl acetate in decorated rooms were significantly higher than those in un-decorated rooms and the related national standards within half a year after decoration. The frequencies of micronucleus of mice exposed to tested VOCs with concentration being 40 times as high as those in decorated rooms were significantly higher than those of negative control group at the 15 th day after exposure. There were no significant differences in frequencies of mi-cronucleus in peripheral blood lymphocytes between waitress working in decorated rooms and un-decorated rooms. Conclusion VOCs emitted from decoration materials were uneasily diffused in air-conditioned airtight environment. The higher concentrations of VOCs simulated based on their levels in decorated rooms revealed mutagenecity to ex-perimental animals.

Investigation on the Pollution of Cryptosporidiumparvum and Giardia lamblia Stiles in Drinking Water and Wastewater in Shenzhen / 环境与健康杂志

Objective To investigate the pollution of parasitic protozoa in drinking water and wastewater in Shenzhen in or-der to present evidence to establish the national hygienic standard for parasitic protozoa in drinking water.Methods Water samples of resource water,fin ished wa ter from3water plants,and post-treated wastewater samples from3wastewater treatment plants in Shenzhen city were collected.Para sitic pro tozoa Giardia lamblia Stiles and Cryptosporidiumparvum were detected by following steps:filtering,washing,magnetic isolation and staining.Results Giardia lamblia Stiles and Cryptosporidiumparvum were not found in samples from raw water and finished water,while they were found in post-treated wastewater samples from2wastewater treatment plants.Conclusion The results indicated that the prob lem of Giardia lamblia Stiles and Cryptosporidi -umparvum pollu tion in the water supplying systems didnt exist at present,but Giardia lamblia Stiles and Cryptosporidi umparvum in the post-treated wastewater were the potential pollution source to surface water.

Air Pollutants Change in Shenzhen, China in 2002-2005 / 环境与健康杂志

Objective To study the change tendency of air pollutants in Shenzhen, China in 2002-2005, further, to explore the relationship between disease spectrum and air pollution. Methods The daily mean concentration of SO2, NO2 and PM10 in 2002-2005 in eight environmental monitoring sites were determined according to Ambient Air Quality Standard. Results Air concentrations of SO2, NO2 and PM10 increased yearly in 2002-2004, from 0.018 3 mg/m3 to 0.023 4 mg/m3, 0.050 1 mg/m3 to 0.072 5 mg/m3, 0.061 0 mg/m3 to 0.075 7 mg/m3, respectively, however, the concentrations of the three indexes decreased in 2005. In the past four yeas, average levels of SO2 were under the limit of grade 2 of GB3095-1996, as for PM10, under the limit of grade 2, and most of the concentrations of NO2 were under the limit of grade 3, but in the other two monitoring sites, the concentrations of NO2 in 2004 exceeded the limit of grade 3. The year average concentrations of SO2, NO2 and PM10 in 2002-2005 in the outer region were higher compared with those in the inner region of the special economic zone, except the level of NO2 in 2002. As for the quarter average concentrations of SO2, NO2 and PM10, 1st and 4th were higher compared with 2nd and 3rd. Conclusion The air quality in Shenzhen is better in China, however, more attention should be paid to the pollution of nitrogen oxides and inhalable particle matters.

Investigation on Contamination Situation of Centralized Air Condition Systems in Hotels in Shenzhen City / 环境与健康杂志

Objective To know the contamination situation of the centralized air conditioning-ventilating systems in hotels in Shenzhen City, Guangzhou province and to ensure the indoor air quality when the centralized air conditioning-ventilating systems were at working. Methods 22 hotels were selected to determine the total count of bacteria and fungi in internal surface of pipes and air blow, the dust volume of internal surfaces of pipes, inhalable particulate matter(PM10) in air blow, and Legionellae in cooling tower water. Results The eligibility percent of dust volume, the total number of bacteria and fungi in internal surface was 94.76 %, 95.71% and 91.90 % respectively,the eligibility percent of PM10, the total count of bacteria and fungi in air blow was 42.54%, 94.76% and 84.29% respectively, and the detected rate of Legionellae was 57.50%. Conclusion The pollution in some degrees has been observed in the centralized air conditioning-ventilating systems in hotels of Shenzhen City, so it is considered as the important measure to improve indoor air quality and ensure people health to strengthen management of centralized air conditioning-ventilating systems in the usage.

Effects of Meteorological Factors on SO_2 and Other Atmospheric Pollutants in Shenzhen,China / 环境与健康杂志

Objective To explore the effects of the meteorologic factors on air pollutants and to establish a model of relationship between the meteorologic factors and concentration of air pollutants. Method The data of SO2,NO2 and PM10 concentration in the air and the related meteorologic factors (daily average,maximum and minimum atmospheric pressure and daily atmospheric pressure difference,daily average,maximum and minimum air temperature,monthly rainfall,daily average and minimum relative humidity and wind speed) from January 2002 to December 2007 were collected. The correlation between the air pollutant levels and related meteorologic factors was analyzed by the multiple regression models with SPSS 11.5 software and multiple regression equations were established. Results Minimum relative humidity was negatively correlated with the concentration of SO2 and NO2 in atmosphere . Daily minimum air temperature was negatively correlated with the concentration of NO2 in atmosphere. Daily average relative humidity and wind speed were negatively correlated with the concentration of PM10 in atmosphere. Daily atmospheric pressure difference was positively correlated with the concentration of PM10 in atmosphere. Multiple regression equations of the concentration of SO2,NO2 and PM10 in atmosphere and meteorologic factors have statistical significances. Conclusion The meteorologic factors have some effects on the air pollution levels. By using the correlation of meteorologic factors and the concentration of air pollutions,multiple regression equations can be established.

Investigation on Water Source Pollution by Cryptosporidium parvum and Giardia lamblia in the Small Towns and Villages in Shenzhen,China / 环境与健康杂志

Objective To investigate the water source pollution by parasitic protozoa in small towns and villages in Shenzhen,China,in order to present the data to establish the national hygienic standard for controlling parasitic protozoa pollution.Methods The samples of water source were collected from 8 village level water plants in Shenzhen.Giardia lamblia and Cryptosporidium were detected,according to the EPA methods,by filtering,rinsing,magnetic isolation and staining.Results Giardia lamblia was found in water sources of 6 water plants and Cryptosporidium was found in one water plant.Conclusion At present,some water sources of village level water plants where the reservoirs were used as the water source in Shenzhen have been polluted by Giardia lamblia and Cryptosporidium,that indicates the potentiality of the water-born-diseases in that areas.