Effects of the expression of miR-375 on the proliferation and invasion of choroidal melanoma cells / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the effect of the expression of miR-375 on the proliferation and invasion of choroidal melanoma(CM)MUM-2B cells.METHODS: MUM-2B cells were cultured and were transfected with miR-375 mimic sequence(mimic group), miR-375 inhibitor sequence(inhibitor group), negative control group and no treatment(blank group). The qRT-PCR, CCK-8, apoptosis and Transwell experiments were used respectively to detect the expression of miR-375, cell proliferation activity, apoptosis, cell migration and invasion.RESULTS: Compared with the negative control group(1.01±0.10)and the blank group(1.03±0.07), the expression level of miR-375 in the cells of the mimic group(2.65±0.15)was increased, while the expression level of miR-375 in the cells of the inhibitor group(0.28±0.06)was decreased(P<0.05). Compared with the blank group and negative control group, the OD values of the cells in the mimic group at 24, 48, 72, and 96h were decreased(P<0.05), while the OD values of the cells in the inhibitor group at 24, 48, 72, and 96h were increased(P<0.05). Compared with the apoptosis rates in the blank group and negative control group, which were(20.54±4.01)% and(22.80±4.28)%, the apoptosis rate in the mimic group(39.11±3.37)% was increased(P<0.05), while it was decreased in the inhibitor group(10.13±2.17)%(P<0.05). Compared with the blank group and negative control group, the number of migration cell and the number of invasion cell in the mimic group were decreased(P<0.05), while the number of migration cell and the number of invasion cell in the inhibitor group were increased(P<0.05). CONCLUSIONS: Up-regulating the expression of miR-375 in MUM-2B cells can reduce cell proliferation activity, accelerate cell apoptosis, and inhibit cell migration and invasion, while down-regulating the expression of miR-375 has the opposite effect. It indicates that miR-375 may play the function of tumor suppressor in the course of CM.

Diagnosis of Bening and Malignant Breast Solid Lesions Using Shear Ware Elastography and Color Image Scoring / 昆明医科大学学报

Objective To investigate the value of color image scoring with shear wave elastography in the diagnosis of benign and malignant breast solid lesions.Methods From February 2016 to February 2017 , 125 patients with breast lesions were given shear wave elastography score, by type of image scoring method, image color method for differential diagnosis between benign and malignant lesions. Results Among all the 125 patients with breast solid lesions, 175 were solid lesions, of which 85 cases (119) were benign lesions, accounting for 68%, and 40 (56) were malignant lesions, accounting for 32%. The difference of type and color score between benign breast lesions and malignant lesions was statistically significant (P＜0.05) . A positive correlation was fourd between color image type score and color score of benign and malignant breast lesion,using shear wave elastography (P＜0.05) . The pathological findings were used as a gold standard, and the difference in the area under ROC curves of benign and malignant lesions of the breast was statistically significant by using type and color scoring method (P＜0.05) . Type scoring method and color scoring method have higher diagnostic efficacy when the diagnostic value is above 2 points. Conclusion The shear wave elastography color image scoring method is of high clinical value in the diagnosis of benign and malignant breast solid lesions, and it is of high accuracy for the diagnosis of benign breast solid lesions.