Detection of staphylococcus aureus enterotoxin genes A-E

The main cause of spreading staphylococcal infections among patients is the healthy carriers working in hospitals. With the secretion of different sorts of toxins such as entrotoxin, this bacteria can provide the conditions for attacking on the host. The main objective of this study is identification of the characteristics and differences in the Staphylococcus aureus isolated from healthy carriers and from the patients on the basis of enterotoxin genes [sea-see]. One hundred and twenty of the patients and 80 of healthy carriers worked in health centers of Gorgan, north of Iran, were investigated for S. aureus isolate. The isolates were evaluated by PCR for Enterotoxin Genes A-E [SEA to SEE]. Enterotoxin genes [SEA to SEE] was found in 87.5% of the total isolates and the most frequent one was enterotoxin gene sea [N= 124]. The prevalence of these isolates in healthy carriers was significantly higher than those of the patients. Based on the results, the high percentage of S. aureus isolated from clinical samples contains enterotoxin genes. Therefore, Human as the source and carrier of S. aureus is paramount importance, which is due to significant relationship between being toxigenic strains and the source of isolation

[ antibacterial effects of 20 herbal plants on methicillin resistant and sensitive s.aureus in Golestan provience]

Staphylococcus aurues is on important cause of community and hospital- aquired infections. Caused by methicillin or oxacillin- resistant s.aureus [MRSA] are mainly nosocomial and are increasingly from many countries word wide. Many attempt have been made by the reasearchers to find new compounds as a subsitute for this antibiotics. The aim of this study was to investigate the antimicrobial activity of alcoholic extracts of 20 medical plants species of Golestan provience on clinical and standard strains of MRSA and MSSA and comprative and detect the best medical plant. In this study the compounds of the plant were extracted by percolation method and the effect of ethanolic extract of 20 Iranian medical plants against methicillin resistant and methicillin sensitve strains were assessed by disc diffusion method and each test were repeated 3 times and mean inhibition zone were recorded and then, the minimum inhibitory concentration [MIC] of the extracts, that show good inhibition zone in disc diffusion method, was determined by the micro broth dilution method. The results of antibacterial activity of the ethanolic extracts of 20 plants revealed that, the ethamlic extracts of 8 plants have the best effect on strains and the maximum mean inhibition zone was 22.4 mm and the lowest MIC of plants was 0.01 mg/ml. The result of this study indicate that, ethanolic extract of Eucalyptus. Global, Peganum.hermla, Punica.granatum, Berberis.vulgaris, Ttamarixaphylla, Nnigella.sativa, Hypericum.perforatum AND, Artemisia. Herbaalba have the best antibacterial effect against MRSA andMSSA. The result obtained from these plants might be considered sufficent for further study