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Objective:To investigate the clinicopathological features, immunophenotype, diagnosis and differential diagnosis of mammary analogue secretary carcinoma of salivary gland (MASC) .Methods:From Jan. 2018 to Jan. 2021, 28 cases of salivary gland MASC were collected in Ningbo Diagnostic Pathology Center, and 10 cases of acinar cell carcinoma and 10 cases of adenoid cystic carcinoma were selected as controls. Immunohistochemical envision method was used to detect the expression of S-100,, DOG-1, CD117, SOX-10, Mammaglobin and Vimentin, and fluorescence in situ hybridization was used to detect the fusion gene of ETV6-NTRK3.Results:The S-100 protein, SOX-10 and Vimentin of MASC of salivary gland were diffusingly positive (28/28) , Mammaglobin (22/28) and CD117 (19/28) were partially positive, and DOG-1 was negative. ETV6-NTRK3 fusion transcription was successfully detected in 26 of 28 salivary gland MASC cases, of which 23 were positive and 3 were negative.Conclusions:Salivary gland MASC is a low-grade malignant epithelium tumor. Comprehensive detection of the expression levels of S-100 protein, SOX-10, DOG-1, Mammaglobin and CD117 is of great value for the diagnosis and differential diagnosis of MASC. FISH detection of ETV6-NTRK3 gene fusion has important reference value for definite diagnosis.
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Objective To investigate the clinicopathological features and prognoses of papillary glioneuronal tumor (PGNT), and to improve the understanding of clinical worker on the tumor. Methods Three patients with PGNT got conformation in our center from July 1, 2011 to Apral 20, 2019, were chosen. The clinical features, histopathological characteristics, immunohistochemical findings, and prognoses of these patients were retrospectively analyzed. Results Two patients were female and one was male. Two tumors were located in the lateral ventricle and one in the temporal lobe. All patients presented with headache, and two exhibited dizziness. Imaging showed solid or cystic mass with well-defined boundary. Microscopically, it was characterized by papillary structures covered by single to multiple layers of small cuboidal gliocytes, and neurocyte-like cells scattered between the papilla. Immunohistochemically, the tumor cells covering papilla were positive for glial fibrillary acidic protein and oligodendrocyte transcription factor 2, and neurocyte-like cells were positive for synaptophysin; and the tumor cells were negative for isocitrate dehydrogenase 1 and silk/threonine specific protein kinase gene BRAF V600E. The proliferating cell nuclear antigen Ki-67 labeling index ranged from 1％-2％. Three patients were followed up for 12, 24 and 48 months after tumor resection, respectively, and no recurrence was found. Conclusion PGNT is an uncommon and variant of mixed neuronal-glial neoplasm with low-grade malignancy; the prognosis is good after gross total resection.
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Objective To determine the effect of intramuscular administration of rh-GH on myocardial angiogenesis and the expression of bFGF, VEGF in rats with acute myocardial infarction. Methods Left anterior descending coronary arteries have been ligated in 50 Wistar rats after anesthesia by 10% pentobarbital sodium. 37 rats with AMI survived after the operation. The rats are randomized into rh-GH treatment group (n=19) and the control group (n=18). Treatment group is given rh-GH 0.25 U/kg?d im for three weeks while the control group is given NS im at the same time. All the 37 rats are killed after 3 weeks and the heart specimen were fixed by injecting 10% formalin from the ascending aorta into the coronary arteries. The level of VEGF and bFGF in blood plasma and myocardium as well as the density of newly formed vessels in the peri-infactional area were measured separately. Plasma VEGF and bFGF were measured before experiment and 3 weeks after experiment by enzyme-label method.bFGF and VEGF in myocardiun were semi-quaot fixed by imaging after SP inmuno-histochemical stain. Left ventricle capillary density: 3 angiogenesis sites was identified by 40? or 100? microscopy. F8 labelled microvessels were measured averagely under 200? microscope. Results (1) Plasma VEGF and bFGF in both groups were elevated, and elevation of the treatment group was more remarkable than the control group 69?5 vs 36?4 (P