Effects of Banxia Baizhu Tianma Decoction on Expression of miRNA-146a-5p in Hippocampus of Epileptic Rats and Bioinformatic Analysis / 中国中医药信息杂志

Objective To lay the foundation for studying the possible pathogenesis of epilepsy and the anti-epileptic mechanism of Banxia Baizhu Tianma Decoction through the bioinformatic analysis of target gene prediction and signal pathway of miRNA-146a-5p in hippocampus of epileptic rats. Methods Lithium-pilocarpine was used to induce seizures in rat models. The experiment rats were randomly divided into normal control group, model group, Banxia Baizhu Tianma Decoction group, with 20 rats in each group. The method of miRNA expression profiling was used to observe the miRNA differential expression of hippocampus neuron cell of rats. The expression level of miRNA-146a-5p was detected by real-time quantitative PCR. MiRDB was used for target gene prediction of miRNA-146a-5p, and miRTarBase and DAVID were used for enrichment analysis on the GO function and KEGG signaling pathway. Results The attack times and grades of the rats in Banxia Baizhu Tianma Decoction group were significantly lower than those in the model group from behavioral observation. MiRNA microarray analysis showed that the expression level of miRNA-146a-5p in model group was 2.107 times normal control group (P＜0.05), and the expression level decreased to 1.377 times after treatment with Banxia Baizhu Tianma Decoction (P＜0.05). The results of RT-PCR was consistent with that of miRNA microarray, with statistical significance (P＜0.05). MiRNA-146a-5p target gene prediction results had 140 target genes by GO, and there were 14 annotation information of biological process (P＜0.05), 9 annotation information of cellular component (P＜0.05), 11 annotation information of molecular function (P＜0.05). Enrichment analysis of KEGG biological pathway showed that 140 target genes of miRNA-146a-5p were enriched in EB virus infection signal pathway and thyroid hormone signaling pathway (P＜0.05). Conclusion miRNA-146a-5p is closely related to the inflammatory reaction after epilepsy, and Banxia Baizhu Tianma Decoction can control epilepsy possibly by controlling the inflammatory reaction after epilepsy.

Research progress on the effects of epithelial-mesenchymal transition circulating tumor cells in the recurrence and metastasis of ovarian cancer / 解放军医学杂志

Ovarian cancer has become a common gynecological malignant tumor. Most of the patients have caught middle or late stage (stage 3 or 4) disease at presentation with poor prognosis. In despite of experienced optimal cytoreductive surgery and chemotherapy, most patients would be hard to avoid recurrence and metastasis of neoplasm, and recurrence and metastasis is the major cause of mortality in patients. Studies have indicated that circulating tumor cells (CTCs) are related to the recurrence and metastasis of ovarian cancer. Detection of CTCs in the peripheral blood might be helpful to predict prognosis of ovarian cancer, and will be effective indicators for monitoring recurrence and metastasis of ovarian cancer. Thus the detection method of CTCs and its clinical application in ovarian cancer have herewith been briefly summarized in the present paper.

Effects of 45S5 bioglass on surface properties of dental enamel subjected to 35% hydrogen peroxide / 国际口腔科学杂志·英文版

Tooth bleaching agents may weaken the tooth structure. Therefore, it is important to minimize any risks of tooth hard tissue damage caused by bleaching agents. The aim of this study was to evaluate the effects of applying 45S5 bioglass (BG) before, after, and during 35% hydrogen peroxide (HP) bleaching on whitening efficacy, physicochemical properties and microstructures of bovine enamel. Seventy-two bovine enamel blocks were prepared and randomly divided into six groups: distilled deionized water (DDW), BG, HP, BG before HP, BG after HP and BG during HP. Colorimetric and microhardness tests were performed before and after the treatment procedure. Representative specimens from each group were selected for morphology investigation after the final tests. A significant color change was observed in group HP, BG before HP, BG after HP and BG during HP. The microhardness loss was in the following order: group HP>BG before HP, BG after HP>BG during HP>DDW, BG. The most obvious morphological alteration of was observed on enamel surfaces in group HP, and a slight morphological alteration was also detected in group BG before HP and BG after HP. Our findings suggest that the combination use of BG and HP could not impede the tooth whitening efficacy. Using BG during HP brought better protective effect than pre/post-bleaching use of BG, as it could more effectively reduce the mineral loss as well as retain the surface integrity of enamel. BG may serve as a promising biomimetic adjunct for bleaching therapy to prevent/restore the enamel damage induced by bleaching agents.

Effect of acupuncture combined language training on cerebral palsy children with language retardation / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe effects of acupuncture combined speech therapy for cerebral palsy children with linguistic retardation.</p><p><b>METHODS</b>Totally 132 cerebral palsy children were randomly assigned to the speech training group (Group A, 44 cases) and the routine acupuncture combined speech training group (Group B, 44 cases), and the acupuncture combined speech training group (Group C, 44 cases). Patients in Group A received one to one training including game therapy, therapy of communication attitudes, and so on. Those in the other two groups were needed at Baihui (GV20), Sishencong (EX-HN1), the first language zone, the second language zone, and the third language zone. Those in Group B were treated with electric needling and then speech training. Those in Group C were treated with language training, while needling with needle maintaining for 40 min. All patients were treated once daily, 5 times per week, 20 times as one course of treatment, 6 courses in total. The efficacy was assessed using S-S phonetic speech developmental retardation examination (CRRC version). The development quotient (DQ) was observed referring to the Gesell intellectual development scale before treatment, after 3 and 6 treatment courses.</p><p><b>RESULTS</b>Compared with Group A (the total effective rate: 51.3%, DQ value: 58.1 +/- 13.3), better effects were obtained in Group B (the total effective rate: 77.5%, DQ value: 60.4 +/- 13.5) and Group C (the total effective rate: 81.0%, DQ value: 64.0 +/- 11.6) (all P < 0.05). There was no statistical difference in the total effective rate or post-treatment DQ value between Group B and Group C (P > 0.05).</p><p><b>CONCLUSION</b>Acupuncture combined speech therapy showed obvious effects on cerebral palsy children with linguistic retardation.</p>

Advances of studies on acupuncture and moxibustion for treatment of language disorder in children with cerebral palsy / 中国针灸

The literatures about the therapeutic effect of acupuncture and moxibustion on language disorder in children with cerebral palsy were reviewed. The symptoms of language disorder in children with cerebral palsy and the procedures of rehabilitation treatments were analyzed. The acupuncture intervention on language disorder in children with cerebral palsy was investigated. It showed that the acupuncture intervention may markedly improve the condition of language disorder in children with cerebral palsy. However, the effectiveness is compared between comprehensive therapy and single therapy in the current literatures on acupuncture intervention. Therefore, the compa rison between comprehensive therapies was lacked, and the advantage of acupuncture intervention combined with other therapies was not demonstrated. In the future, the studies should focus on the combination of various therapies and the different effectiveness of different therapies. Furthermore, the studies should focus on the mechanism of acupuncture. Therefore, it may contribute to build a comprehensive treatment system in which acupuncture is dominatant for cere bral palsy in children.

Thrombolysis of rabbit's pulmonary embolism with thrombus-targeted urokinase immune liposome / 中华心血管病杂志

<p><b>OBJECTIVE</b>To develop thrombus-targeted urokinase immune liposome through incorporating D-dimer monoclonal antibody (DDmAb) to liposome and observe the thrombolytic efficiency in a rabbit pulmonary thromboembolism (PE) model.</p><p><b>METHODS</b>Reverse-phase evaporation method was used to develop targeted urokinase immune liposome by coupling DDmAb to urokinase liposome (liposomal-encapsulated urokinase) with glutaraldehyde. The PE models were induced by injecting 4 autologous emboli (2 mm x 5 mm) through jugular vein catheter into pulmonary arteries. New Zealand white rabbits (n = 32) were randomized into four groups: A group (TBS), B group (150 000 IU/kg UK), C group (30 000 IU/kg urokinase liposome) and D group (30 000 IU/kg urokinase immune liposome). The right ventricular pressure and the emboli size in pulmonary arteries were determined.</p><p><b>RESULTS</b>The right ventricular pressure increased significantly in PE rabbits (P < 0.01), the average value is (6.75 +/- 6.82) mm Hg (1 mm Hg = 0.133 kPa). Eighty minutes post various treatments, right ventricular pressure remained unchanged as post PE in group A [(40.15 +/- 11.22) mm Hg vs. (41.67 +/- 14.23) mm Hg], decreased to baseline level in group B and D [(34.71 +/- 8.67) mm Hg vs. (33.98 +/- 9.32) mm Hg, (30.65 +/- 6.67) mm Hg vs. (30.77 +/- 6.85) mm Hg, all P > 0.05], decreased but not returned to normal value in group C. Residual emboli size remained unchanged in group A and partly reduced in group C and more significantly reduced in group B and D. Hemorrhage of heart, kidney and liver was evidenced in group A but not in other groups.</p><p><b>CONCLUSION</b>Acute PE could be successfully treated by the thrombus-targeted urokinase immune liposome with D-dimer monoclonal antibody.</p>

In vivo noninvasive detection of vulnerable atherosclerotic plaque by (99)Tc(m)-Annexin V imaging in an atherosclerotic rabbit model / 中华心血管病杂志

<p><b>OBJECTIVE</b>Apoptosis contributes to the instability of the atherosclerotic (AS) lesions. The vulnerable plaque was identified in vivo by detecting the apoptosis with radiolabeled annexin V in an atherosclerotic rabbit model.</p><p><b>METHODS</b>Eight male New Zealand white rabbits on 2% cholesterol diet for 2 weeks had abdominal aortic balloon injury and fed a 2% cholesterol diet for another 15 weeks (AS group), 3 rabbits fed a normal rabbit chow for 17 weeks without balloon injury served as controls. Annexin V labeled with (99)Tc(m) was then intravenously administered and planar whole-body images were captured using a gamma camera in the left lateral position. The entire length of the abdominal aorta was explanted for ex vivo imaging with gamma camera. The aorta then was divided into several segments according to the severity of AS. The segments were separated weighted and counted in an gamma counter for the absorptive dose of annexin per gram of tissue. Histology examinations were made on specimens.</p><p><b>RESULTS</b>At 2 hours post annexin V injection, clear delineation of radiolabel within the abdominal aorta could be evidenced in vivo gamma imaging. After explanation of the aorta, ex vivo imaging showed a robust uptake of radiotracer in the infradiaphragmatic aorta corresponding to the in vivo images and conforming to the macroscopic distribution of atherosclerotic lesions. The uptake of radiolabel was absent in areas without grossly visible atherosclerotic lesions. The in vivo and ex vivo images identified plaque areas were identical and corresponded histological results on the explanted specimen. The aortic specimen was divided into 18 segments on lesions. The magority of the lesions (14/18) manifested as type IV or type V lesions of AHA classification (vulnerable lesions), except segments 1 - 4, which manifested as type I or type II lesions. The thickness of fibrous cap (TFC) and the ratio of cap and lipid nuclear (RCN) were significantly reversely correlated to the unit radioactivity counts, and the correlation between RCN and the unit radioactivity counts was more significant than that between TFC and the unit radioactivity counts (r = -0.904, P < 0.01, and r = -0.8, P < 0.01). Apoptosis detection (TUNEL): annexin V intake in plaques was positively correlated to apoptotic index(r = 0.651, P = 0.012).</p><p><b>CONCLUSION</b>Noninvasive Annexin V imaging could be used to detect vulnerable atherosclerotic plaques in vivo.</p>

Randomized controlled study on AMIE methods for treatment of movement disorder in the child of convulsive cerebral palsy / 中国针灸

<p><b>OBJECTIVE</b>To observe the curative effect of AMIE methods on movement disorder in the child of convulsive cerebral palsy (CP).</p><p><b>METHODS</b>One hundred and twenty cases of CP children were randomly divided into an integration treatment group and a rehabilitation group, 60 cases in each group. The integration treatment group were treated with acupuncture (A), massage (M), injection (I) and five animal exercise (E) therapies for 60 times, and the rehabilitation group were treated with Bobath training therapy for 60 times. Scores for movement function before and after treatment were used for assessment of therapeutic effect. Twelve months later, understand whether or not the children can walk on ones own.</p><p><b>RESULTS</b>The total effective rate was 76.70% in the integration treatment group and 58.4% in the rehabilitation group, with no significant difference between the two groups (P > 0.05); there were significant differences in the two groups in differences of movement function scores before and after treatment (P < 0.05). After one year's follow-up survey, 36 children could walk by themselves in the integration treatment group, which were significantly more than 24 children in the rehabilitation group (P < 0.05).</p><p><b>CONCLUSION</b>AMIE methods is effective for treatment of movement disorder in the child of convulsive cerebral palsy, and the short-term therapeutic effect is same as that of Bobath training method and the long-term therapeutic effect is better than that of Bobath training method.</p>

Regulation of estrogen and phytoestrogen on the dopaminergic systems of amygdala in rats / 生理学报

In vivo fast cyclic voltammetry (FCV) was used to investigate dopamine (DA) release from amygdala (Amy) of female rats in different phases of estrus cycle, ovaricectomized (OVX) rats and male rats. Tyrosine hydroxylase (TH) immunohistochemistry was employed to measure the numbers of immunoreactive neurons in ventral tegmental area (VTA) of midbrain in the rats. We also observed the effect of intracerebroventricular injection of phytoestrogen-soy isoflavones on DA release from the Amy. The results are as follows: DA release from the Amy of proestrus female rats was apparently higher than that in estrus, metaestrus, diestrus female rats and OVX rats. Amy DA release and the numbers of the TH immunoreactive neurons in VTA showed a significant sex difference. DA release from Amy of female and OVX rats increased significantly within 5 min after i.c.v injection of soy isoflavones, which elicited no effects in male rats. The above-mentioned results suggest that endogenous estrogen may play an important role in regulating the activity of DA neurons in mid-limbic systems, and that soy isoflavones exert an estrogen-like effect on the dopaminergic systems in the Amy.