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Objective To investigate the cognition level of clinical nurses to peripheral venous indwelling needles, and provide a reference for nursing administrators to carry out targeted intravenous catheter standardized training operation. Methods By convenience sampling, 129 clinical nurses of the certain grade A class three hospital were selected, and their perception about indwelling intravenous catheter were investigated by using self-designed questionnaire in November 2015. Results 125 questionnaires were effectively rewnered. Average score of 125 nurses′cognition to indwelling intravenous catheter was (57.52 ± 10.23) points, pass rate was 65.6%(82/125), and influencing factors were education background, the job tittle, work experience and so on. Conclusions The clinical nurses′assessment, cognition and knowledge master to indwelling intravenous catheter is not ideal, lacking a comprehensive clinical peripheral venous catheter assessment tools. Clinical nurse managers should focus on strengthening vein management, reasonable and better trained clinical nurse intravenous catheter cognitive level, so as to ensure that the effect of catheter using.
RESUMO
Objective To extract phycobiliproteins from Porphyra haitanensis,find out the optimum extraction conditions and investigate the antioxidation activity of phycobiliproteins in vitro and anti-oxygen free radical in vivo.Methods Porphyra haitanensis was extracted with deioned-water at 40 volumes for 6 days and tissue-triturated for 2 minutes.The crude phycobiliprotein(CPP) was purified by precipitating with 45% ammonium sulphate and dialyzing.The scavenging activities of the crude phycobiliprotein on?OH of the system of 1,10-phenanthroline-Fe(Ⅱ)/H2O2 and O-2? of pyrogallol autoxidation were studied.And the mice were treated by gastric incubation with crude phycobiliprotein,at doses of 100,200,300mg?kg-1?d-1.The activities of erythrocyte catalase(CAT) and glutathione peroxidase(GSH-Px),serum superoxide dismutase(SOD) and the content of erythrocyte MDA in mice were determined on the 25th day after treatment.Results CPP could scavenge free radicals,and also can increase the activities of erythrocyte CAT,GSH-Px,serum SOD and decrease the content of MDA significantly.Conclusion The crude phycobiliprotein from Porphyra haitanensis has antioxidation activity in different ways.