Establishment of zebrafish calculation model and calculation of relative dose coefficients / 中华放射医学与防护杂志

Objective:To assess impact of radiation from nuclear power on the surrounding environment, a dose assessment model was constructed and relevant dose coefficients were determined through refined animal models.Methods:Zebrafish is one of the most important aquatic model animals in the radiation hazard assessment of nuclear power liquid effluent. A geometric model of zebrafish containing internal bones and visceral organ was established for dose estimation. The internal and external dose coefficients (DC) of the zebrafish model were calculated by Monte Carlo method with seven nuclides as the source term, 3H, 40K, 58Co, 60Co, 110Ag, 134Cs, 137Cs, which are common in nuclear liquid effluents and environmental monitoring. Results:The level of nuclide gamma energy determines dose coefficients for external radiation. The dose coefficients of most nuclides in internal organs was higher than that in whole body, and the internal organ dose of 58Co was 165% higher than that in whole body. The internal radiation dose coefficients of the model established in this study was relatively high, and the internal radiation dose coefficients caused by 60Co was 2.6 times of existing ellipsoid model, which suggested that the different internal materials and the choice of different particle types would affect the energy deposition. Conclusions:Refining of model animals is important. Accurate assessment of the organs dose coefficients of model animals is helpful to assess the radiation effects on non-human species.

Study of arginine in combination with leptin in treatment of pancreatitis / 中国综合临床

Objective To investigate the effects of arginine combined with leptin in treatment of rats with a-cute pancreatitis. Methods Sixty Wister rats were randomly divided into sham operation group (group A), acute pancreatitis group (group B),arginine group (group C),leptin group (group D),arginine plus leptin group (group E),with 12 rats in each group. Acute panereatitis model was developed by retrograde injection of 5% sodium tauro-cholate into cholangiopancreatography in rats. After 6 hours, medicine were given by intraperitoneal injection, ab-dominal aortic blood and pancreatic specimens were taken for measurement of tumor necrosis factor-α(TNF-α), ser-um amylase,nuclear factor-κB (NF-κB) activity of pancreatic tissue, and pathological damage score of pancreatic tissue. Results TNF-α,Amylase,NF-κB activity, pathologic scores were(1.206±0.446)μg/L, (1617.2±18.8) U/L,0.174±0.013,0.6±0. 2 in group A;(3.201±0.321) μg/L, (4643.8±303.4) U/L,0.397±0. 025,7.8±1.3 in group B;(1.845±0.404) μg/L,(3370.9±173.2)U/L,0.255±0.026,3.9±1.1 in group C;(1.996±0.374) μg/L, (3693.7±208.0)U/L,0.274±0.062,4.2±1.2 in group D;(1.440±0.287)μg/L, (2501.0±771.4)U/L,0.211±0.027,2.3±1.0 in group E. Compared with group A, the indicators were significantly in-creased in group B (P < 0.05). Compared with group B, these indicators were reduced in group C and D (P <0.05). There was no significant difference between group C and group D. These indicators in group E were signifi-cantly lower than those in group C and D (P < 0.05). NF-κB in pancreatic tissue were positively correlated with other indicators(P <0.01). Conclusions In treatment of acme pancreatitis in rats, arginine combined with leptin has idenditfied effect, and the effect of combination therapy is better than that of separate medicine.

Treatment of giant cell tumor bone defect near knee joints with allograft bone grains and autograft bone / 中国矫形外科杂志

[Objective] To investigate the treatment in the use of allograft bone grains combined with autograft bone grafting in giant cell tumor.[Method]From March 1996 to March 2006,24 cases of giant cell tumor closed to knee were verified by pathology in this department,including 11 on thighbone and others on tibia.Before operation allografts was sheared to grains after antigen extracted by repeat freeze thawing and mechanical methods.Giant cell tumor of bone was completely erased in surgical fringe and the defect was thoroughly closed-up with allograft grains combined with cancellous bone autografts.The knee joints exercise was made for 12 weeks with external orthosis fixation.[Result]No severe complication occurred in early stage after operation in the 24 patients.All of them were followed up for 1 to 6 years with an average of 2 years and 8 months.None of them recidivated after the operation,and no insert was taken off for severe rejection,one case showed tolerant pathologic fracture.All the transplantations showed ideal appearance in X-ray photograph and the motion of joints had no significant difference than before.Curative effects were evaluated by Mankin criteria.The outcome was excellent in 20 cases,good in 4 cases.[Conclusion]Bone allograft grains combined with autograft is an effective and few rejection in filling of giant bone defect in surgical operation of giant cell tumor near knee joints.

Ultrasonic bone density in normal children aged 6 to 12 years in Shenzhen City / 中国组织工程研究

BACKGROUND: The normal clinical index and the standard error of ultrasonic bone density in the population of normalchildren aged 6-12 years have not yet been established. OBJECTIVE: To investigate the status of ultrasonic bone density in the population of normal children aged 6 to 12 years old in Shenzhen City and establish a normal reference index of ultrasonic bone density of this population. DESIGN: Cross-sectional survey. SETTING: Ultrasound Department of People's Hospital in Longgang District of Shenzhen City. PARTICIPANTS: The volunteers for ultrasonic bone density detection were chosen froma kindergarten, an elementary school and a middle school between March 2002 and April2003. Excluded were those who had bone fracture and received medication of hormone and other drugs within 6months that affected bone metabolism, and menstrual history. Altogether we chose 697 normal children, 367 boys and 330 girls whose age ranged from 6 to 12 years old. METHODS: SAHARA ultrasonic bone density apparatus (the US) was used, and corrected according to the standard body model after the operation began.The error of precision was below 1％ and the error of accuracy was 3％. Bone density of the left heel of the children was detected and body mass (kg) and height (cm) were also measured using the same height and weight scale. MAIN OUTCOME MEASURES: The correlation between bone densityof the heel and sex, age and body mass in the population of normal children aged 6 to 12 years. RESULTS: The normal reference values of bone density in the population from 6 to 12 years old were (0.445±0.166) g/cm2 in 6-year-old group,(0.509±0.151) g/cm2 in 7-year-old group, (0.510±0.133) g/cm2 in 8-year-old group, (0.519±0.132) g/cm2 in 9-year-old group, (0.520±0.153) g/cm2 in 10-year-old group, (0.53±0.175) g/cm2 in 11-year-old, and (0.545±0.206) g/cm2 in 12-year-old group. There were differences in bone density between boys and girls (P ＜ 0.001), and after correction by body mass, the difference disappeared. Bone density of the boys and girls had increasing linear correlation with age (male r=0.722, P ＜ 0.001; female r=0.785, P ＜ 0.001), and had significant relationship with body mass (r=0.984, P ＜ 0.001). CONCLUSION: In normal children aged 6 to 12 years old, bone density of the heel has no association with sex, but has increasing linear correlation with age and significant relationship with body mass.

Analysis of the result from vertebral artery of patients with cervical spondylopathy by Ultrasonic doppler technique / 中国实用内科杂志

Objectives To disscuss the changes of structure and hemodynamics of vertebral artery in patients with cervical spondylopathy.Methods Study the changes of structure,systolic flow rate peak value(MAX),diastolic phase flow rate (MIN),diameter(D) upon vertebral artery by Doppler , s ultrasonic diagnosis apparatus with 7.5MHz in 29 patients with cervical spondylopathy and 28 healthy subjects.Results The arcuation of vertebral artery in patients with cervical spondylopathy distinctly increase.D?MAX?MIN have significantly difference (P