Bax inhibitor 1 inhibits vascular calcification in mice by activating optic atrophy 1 expression / 南方医科大学学报

OBJECTIVE@#To investigate the effects of Bax inhibitor 1 (BI- 1) and optic atrophy protein 1 (OPA1) on vascular calcification (VC).@*METHODS@#Mouse models of VC were established in ApoE-deficient (ApoE-/-) diabetic mice by high-fat diet feeding for 12 weeks followed by intraperitoneal injections with Nε-carboxymethyl-lysine for 16 weeks. ApoE-/- mice (control group), ApoE-/- diabetic mice (VC group), ApoE-/- diabetic mice with BI-1 overexpression (VC + BI-1TG group), and ApoE-/- diabetic mice with BI-1 overexpression and OPA1 knockout (VC+BI-1TG+OPA1-/- group) were obtained for examination of the degree of aortic calcification using von Kossa staining. The changes in calcium content in the aorta were analyzed using ELISA. The expressions of Runt-related transcription factor 2 (RUNX2) and bone morphogenetic protein 2 (BMP-2) were detected using immunohistochemistry, and the expression of cleaved caspase-3 was determined using Western blotting. Cultured mouse aortic smooth muscle cells were treated with 10 mmol/L β-glycerophosphate for 14 days to induce calcification, and the changes in BI-1 and OPA1 protein expressions were examined using Western blotting and cell apoptosis was detected using TUNEL staining.@*RESULTS@#ApoE-/- mice with VC showed significantly decreased expressions of BI-1 and OPA1 proteins in the aorta (P=0.0044) with obviously increased calcium deposition and expressions of RUNX2, BMP-2 and cleaved caspase-3 (P= 0.0041). Overexpression of BI-1 significantly promoted OPA1 protein expression and reduced calcium deposition and expressions of RUNX2, BMP-2 and cleaved caspase-3 (P=0.0006). OPA1 knockdown significantly increased calcium deposition and expressions of RUNX2, BMP-2 and cleaved caspase-3 in the aorta (P=0.0007).@*CONCLUSION@#BI-1 inhibits VC possibly by promoting the expression of OPA1, reducing calcium deposition and inhibiting osteogenic differentiation and apoptosis of the vascular smooth muscle cells.

Analysis of early objective visual quality and vision related quality of life after ICL V4c implantation for high myopia / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To evaluate the early objective visual quality and vision related quality of life after implantation of posterior chamber phakic implantable collamer lens with a central hole(ICL V4c)for high myopia by applying the double-pass optical quality analysis system Ⅱ(OQAS Ⅱ)and life quality questionnaire.<p>METHODS: Totally 26 patients(44 eyes)with high myopia were enrolled in this research. The patients were all recieved ICL V4c implantation by the same surgeon in our hospital. The evaluation items included uncorrected visual acuity(UCVA), best corrected visual acuity(BCVA), intraocular pressure, corneal endothelial cell density(ECD), vault, objective scattering index(OSI), modulation transfer function cut off frequency(MTF cut off), Strehl ratio(SR), predicted visual acuity values(OV 100%, OV 20%, OV 9%)at contrasts of 100%, 20%and 9% and vision related quality of life questionnaire. All measurements were performed preoperative and 1wk, 1mo, 3mo postoperatively.<p>RESULTS: Compared with preoperative BCVA, for the high myopia patients, the 1wk, 1mo and 3mo UCVA postoperative were better at all time points, and the differences were statistically significant(<i>P</i><0.001). Postoperative 1wk intraocular pressure was higher than that in preoperative and postoperative 1mo and 3mo(<i>P</i><0.05). Postoperative corneal endothelial cell counts at all time points were lower than that in preoperative(<i>P</i><0.05), but all within the normal range. No significant difference was found in vault(<i>P</i>=0.790). Compared with preoperative OSI, MTF cut off, SR, OV 100%, OV 20% and OV 9%, the situation improved at postoperative 1wk, 1mo and 3mo, with statistical significance(<i>P</i><0.001). The vision related quality of life questionnaire showed that all patients had high satisfaction in the good subjective visual acuity after operation.<p>CONCLUSION: ICL V4c implantation is safe and effective in correcting high myopia. The objective visual quality and vision related quality of life of patients with high myopia was significantly improved after ICL V4c implantation in the early stage. The research laid a foundation for the establishment of “the comprehensive evaluation system of subjective and objective combination” of ICL in the application of high myopia.

Analysis of early objective visual quality and vision related quality of life after ICL V4c implantation for high myopia / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To evaluate the early objective visual quality and vision related quality of life after implantation of posterior chamber phakic implantable collamer lens with a central hole(ICL V4c)for high myopia by applying the double-pass optical quality analysis system Ⅱ(OQAS Ⅱ)and life quality questionnaire.<p>METHODS: Totally 26 patients(44 eyes)with high myopia were enrolled in this research. The patients were all recieved ICL V4c implantation by the same surgeon in our hospital. The evaluation items included uncorrected visual acuity(UCVA), best corrected visual acuity(BCVA), intraocular pressure, corneal endothelial cell density(ECD), vault, objective scattering index(OSI), modulation transfer function cut off frequency(MTF cut off), Strehl ratio(SR), predicted visual acuity values(OV 100%, OV 20%, OV 9%)at contrasts of 100%, 20%and 9% and vision related quality of life questionnaire. All measurements were performed preoperative and 1wk, 1mo, 3mo postoperatively.<p>RESULTS: Compared with preoperative BCVA, for the high myopia patients, the 1wk, 1mo and 3mo UCVA postoperative were better at all time points, and the differences were statistically significant(<i>P</i><0.001). Postoperative 1wk intraocular pressure was higher than that in preoperative and postoperative 1mo and 3mo(<i>P</i><0.05). Postoperative corneal endothelial cell counts at all time points were lower than that in preoperative(<i>P</i><0.05), but all within the normal range. No significant difference was found in vault(<i>P</i>=0.790). Compared with preoperative OSI, MTF cut off, SR, OV 100%, OV 20% and OV 9%, the situation improved at postoperative 1wk, 1mo and 3mo, with statistical significance(<i>P</i><0.001). The vision related quality of life questionnaire showed that all patients had high satisfaction in the good subjective visual acuity after operation.<p>CONCLUSION: ICL V4c implantation is safe and effective in correcting high myopia. The objective visual quality and vision related quality of life of patients with high myopia was significantly improved after ICL V4c implantation in the early stage. The research laid a foundation for the establishment of “the comprehensive evaluation system of subjective and objective combination” of ICL in the application of high myopia.

Cell-penetrating Ability and Protective Effect of Recombinant PTD-HSP27 on RGC Against Hypoxia-induced Damage / 中山大学学报(医学科学版)

@#【Objective】To detect the ability and the efficiency of recombinant PTD-HSP27 transport across RGC-5 cells and measure the role of recombinant PTD- HSP27 against oxidative stress damage induced by cobalt chloride on RGC- 5 cells，and explored its potential mechanism tentatively. 【Methods】 RGC- 5 cells were incubated with PTD- HSP27 labeled with FITC，followed by the observation of fluorescence using fluorescence microscope. We also lysed the cells by radio immunoprecipitation assay and measure the transport efficiency of PTD- HSP27- FITC through ultraviolet spectrophotometer subsequently. We established the RGC- 5 cell damage model. Cellular experiments were divided into three groups：normal control group，cobalt chloride damage group，PTD-HSP27 + cobalt chloride treatment group. Those three groups of cells were experimented using Annexin V- FITC/PI staining kit：to detect apoptotic cell ratio；Western Blotting：to detect the expression level of apoptosis related protein，including Bcl-2，Bax and Caspase-3.【Results】The fluorescence of PTD- HSP27- FITC was visualized inside the RGC- 5 cells using the fluorescence microscope，while the transport efficiency of PTD-HSP27-FITC was（47.29 ± 2.33）%. Cobalt chloride inhibited the survival vitality of RGC- 5 cells in a concentration- dependent manner. The comparison among normal control group，cobalt chloride damage group and PTD-HSP27+CoCl2 treatment group showed：PTD-HSP27 could effectively suppressed early apoptosis induced by cobalt chloride（P<0.01）. Western Blotting results showed that PTD-HSP27 could effectively enhance the expression ratio of Bcl-2/Bax，and suppressed the activation of Caspase- 3（P<0.01）.【Conclusions】Recombinant protein PTD- HSP27 could protect RGC- 5 cells against oxidative stress injury induced by cobalt chloride. Iit mainly regulated the expression ratio of Bcl-2/Bax，suppressed early apoptosis and improved cell viability in RGC-5 cells.