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Objective To observe the changes of laboratory blood indexes in patients with intrahepatic cholestasis of pregnancy(ICP),and analyze the value of blood inflammation indexes and liver function indexes in the diagnosis of ICP and the prediction of delivery mode.Methods A total of 251 patients diagnosed with ICP in this hospital from January 2021 to December 2022 were selected as the ICP group,and another 200 healthy pregnant women were selected as the control group.The patients with ICP were further divided into the severe ICP group(n=47)and the mild ICP group(n=204),the vaginal delivery group(n=113)and the cesarean section group(n=138)according to the severity of ICP and delivery mode.Mann-Whitney U test was used for comparison of parameters between groups,and Spearman method was used for correlation analy-sis.Receiver operating characteristic(ROC)curves were used to evaluate the efficacy of laboratory indicators in diagnosing ICP and predicting delivery mode.Results Neutrophil/lymphocyte ratio(NLR)[6.01(4.45,8.37)vs.3.36(4.12,3.51)]and aspartate transaminase(AST)level[20.00(16.00,33.00)U/L vs.15.00(13.00,18.00)U/L]in the ICP group were significantly higher than those in the control group(P＜0.05),and NLR in the severe ICP group was significantly higher than that in the mild ICP group[4.93(3.87,7.35)vs.4.14(3.12,5.17),P＜0.05].Correlation analysis showed that NLR was positively correlated with AST level(r=0.279,P＜0.001)and ICP severity(r=0.139,P=0.028)in patients with ICP.The area under ROC curve(AUC)of NLR combined with AST for ICP diagnosis was 0.882(95％CI:0.851-0.913).In ad-dition,cholinesterase(CHE)[6 020.00(5 499.50,6 703.50)U/L vs.5 341.50(4 651.75,6 259.25)U/L]and prealbumin(PA)[199.00(177.71,225.20)mg/Lvs.169.17(139.18,204.40)mg/L]levels in the va-ginal delivery group were significantly higher than those in the cesarean section group(P＜0.05),and the AUC of CHE combined with PA for predicting vaginal delivery in ICP patients was 0.727(95％CI:0.664-0.789).Conclusion NLR and AST have potential value in the diagnosis of ICP,and CHE and PA have poten-tial value in predicting delivery mode of ICP patients.
RESUMO
@#To study the effects of Roudoukou-8 San against hydrogen peroxide-induced cardiomyocyte in neonatal rats and to explore its mechanism. Cardiomyocytes were isolated and cultivated by neonatal rats and the injure models were established by H2O2. Methyl thiazolyl tetrazolium(MTT)assay was used to detect the protective effects of Roudoukou-8 San on H2O2-induced cardiomyocyte. The effects of Roudoukou-8 San on myocardium morphology were observed under inverted microscope. The contents of lactate dehydrogenase(LDH), creatine kinase(CK)and aspartate aminotransferase(AST)in cell culture medium were determined by Automatic biochemical instrument; The levels of malonydialdehyde(MDA), superoxide dismutase(SOD)and NO in the cells were detected by kit method. The apoptotic morphology of cardiomyocytes was observed by Hoechst fluorescence staining. Cell apoptosis were measured by Annexin V and PI double staining and flow cytomety. 100μmol/L H2O2 for 2 h could cause about 50% of myocardial injury. In the inverted optical microscope, H2O2 model group showed increased cell gap, decreased cell count, cell cytoplasmic vacuoles and other obvious damage. Roudoukou-8 San protected cell from H2O2-induced morphlogical improved in different degrees, reduced the release of LDH, CK and AST content, reduced the content of MDA, NO in myocardial cells significantly and increased the activity of SOD. Roudoukou-8 San energy significantly inhibited H2O2 damage myocardial cell apoptosis. Our study suggested that Roudoukou-8 San can protect cardiomyocyte from H2O2-induced injury by improving the cell viability, reducing oxidative stress injury, inhibiting inflammatory reaction and inhibiting cell apoptosis.