RESUMO
BACKGROUND:The temporal and spatial expression of fibroblast growth factor receptors 1 and 2 remains a controversial issue during kidney development,so the relationship between them and kidney development remains unclear. OBJECTIVE:To observe the dynamic expression of fibroblast growth factor receptors 1 and 2 during kidney development of mice,and to investigate the relationship between them and kidney development. METHODS:The kidneys of fetal mice[embryotic days(E)12,14,16,and 18]and neonatal mice[neonatal days(N)1,3,7,14,24,and 40]were selected to examine the temporal and spatial expression of fibroblast growth factor receptors 1 and 2 by immunohistochemistry method in kidney tissues,and quantitative analysis was performed using western blot assay. RESULTS AND CONCLUSION:(1)Immunohistochemistry showed that fibroblast growth factor receptor 1 was mainly localized in metanephric tissue surrounding the tip of the ureteral bud at E12.Subsequently,fibroblast growth factor receptor 1 was expressed in immature renal corpuscles at various stages,some distal convoluted tubules and capillary loops.The positive site was mainly concentrated in the generative region.Fibroblast growth factor receptor 2 was initially expressed in both ureteral buds and metanephric tissue.Fibroblast growth factor receptor 2 was localized in immature renal corpuscles,distal tubules,collecting ducts and thin segments of medullary loops with kidney development.However,the expression of renal corpuscles was weak.(2)Stereology and western blot assay showed that the expression of fibroblast growth factor receptor 1 was high before birth and gradually decreased after birth,while the expression was very low after N7 day.The expression level of fibroblast growth factor receptor 2 increased gradually with the kidney development and tended to be stable after N7 day.(3)The results exhibit that fibroblast growth factor receptors 1 and 2 are expressed spatially and temporally during kidney development.It is speculated that fibroblast growth factor receptors 1 and 2 may influence nephron development and maturation,and fibroblast growth factor receptor 2 is critical during the formation of ureteral buds and morphology.
RESUMO
This article is a part of a systemical study of the Chinese sternum. A total of 191 cases (male 111 cases, female 80 cases) of Chinese adult sternum werc measured. The chief results obtained are as follows:1. The sternal length is 147.6?1.24 mm in male and 137.54?1.30 mm in fcmale.2. The manubrium length of the sternum is 49.42?0.46 mm in male and 48.20?0.66 mm in female. The maximum width of the sternum is 62.96?0.65 mm in male and 59.61?0.71 mm in female. The minimum width of the sternum is 33.71?0.48 mm in male and 31.97?0.34 mm infemale. The thickness of the sternum is 10.19?0.09 mm in male and 9.97?0.16 mm in female.3. Thc length of the body of the sternum is 100.18?1.15 mm in male and 88.20?1.14 mm in female. The maximum width of the body of the sternum is 39.20?0.51 mm in male and 36.98?0.49 mm in female. The maximum thickness of the body of sternum is 12.43?0.22 mm in male and 11.50?0.21 mm in female.4. The length width index of the sternum is 26.66.5. The length width index of the sternal Body is 39.86.
RESUMO
The authors have measured the humerus in 200 pair (115 males and 85females) of the Chinese adults. The results indicate that fhe differencebetween the male and the female humeruses is significant. The maximumlength averages 31. 01 ? 0. 01cm in male and 29. 29 ? 0. 15cm in female. Theaverage length is 30. 50 ? 0. 09cm in male and 28.75 ? 0.14cm in female.The difference in the diameters of the average length of the upper. middle and lower extremities of both sexes is significant. The averagevalue of Shaft index is 77. 23 ? 0. 44 in male and 76. 62 ? 0. 51 in female. Theaverage value of the caliber index is 20. 37 ? 0. 10 in male and 19. 68 ? 0. 12in female. The average value of the head index is 93. 21 ? 0. 18 in male and93. 55 ? 0. 37 in female. The results of the measurement have been comparedwith data published both home and abroad.
RESUMO
Femurs of 206 Chinese adults (122 males and 84 females) were used for the present study. Their lengths, and torsional and inclinational angles were measured after Martin's method. The results are as follows:1. The Lengths of FemurThe average length of the femur in males is 43.68?2.02 cm. (43.77?2.02 cm. on the left side, and 43.61?2.04 cm. on the right), while that in females is 40.96?2.84 cm. (41.05?2.84 cm. on the left side and 40.93?2.84 cm. on the right). No significant difference has been found between the femoral lengths of both sides; however, there is a significant difference between both sexes.2. The inclinational angles of femoral neckTheir average value in the male is 128.46??6.58?(128.87??6.76?on the left side, and 127.46??6.60? on the right), but that in the female is 130.52??6.44?(130.88??6.10? on the left side, and 130.17??6.80? on the right). No significant difference exists between both sides; however, there is a significant difference between both sexes.3. The Torsional Angles of FemurTheir average value in the male is 11.95??8.89? (14.51??8.08? on the left side, and 9.20??7.75? on the right), whereas that in the female is 12.80??9.07? (15.95??9.63? on the left side, and 9.63??7.31? on the right). There is a significant difference between two sides, but no significant difference between both sexes.4. The relationship between the inclinational and torsional angles on one hand and the changes in the femoral lengths on the other has been studied by dividing the femurs into groups according to their lengths. It has been shown that the changes in the femoral length exert no effect upon the inclinational and torsional angles of the femoral necks, neither in the male nor in the female.Besides, a comparison has also been made between the femurs of both sides and both sexes, between the femoral lengths and the inclinational and torsional angles, and between the variant ranges of the torsional angles of femurs. A discussion on these problems has been made with reference to the racial differences.
RESUMO
This article is a part of a systematical study of the Chinese sternum. A total of 191 cases (111 males, 80 females) of Chinese adult sternum were measured. The chief results obtained are as follows:1. The sternal length is 147.6?1.24mm in the male and 137.54?1.30mm in the female.2. The manubrium length of the sternum is 49.42?0.46 mm in the male and 48.20?0.66mm in the female. The maximum width of the sternum is 62.96?0.65mm in the male and 59.61?0.71mm in the female. The minimum width of the sternum is 33.71?0.48mm in the male and 31.97?0.34mm in the female. The thickness of the sternum is 10.19?0.09mm in the male and 9.97?0.16mm in the female.3. The length of the body of the sternum is 100.18?1.15 mm in the male and 88.20?1.14 mm in the female. The maximum width of the body of the sternum is 39.20?0.51 mm in the male and 36.98?0.49 mm in the female. The maximum thickness of the body of sternum is 12.43?0.22mm in the male and 11.50?0.21 mm in the female.4. The length-width index of the sternum is 26.66.5. The length-width index of the sternal body is 39.86.6. The width-thickness index of the sternum is 31.00.7. The index of the length of the sternal body and the manubrium is 50.00.8. The index of the length of the sternum and the manubrium is 34.38.9. The index of the width and the length of the manubrium is 80.00.